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The map label for this gene is fmt

Identifier: 28378316

GI number: 28378316

Start: 1470265

End: 1471218

Strand: Direct

Name: fmt

Synonym: lp_1616

Alternate gene names: 28378316

Gene position: 1470265-1471218 (Clockwise)

Preceding gene: 28378315

Following gene: 28378317

Centisome position: 44.44

GC content: 46.54

Gene sequence:

>954_bases
ATGACATCAATCGTTTTTATGGGAACGCCCGCGTTTGCGGCACCGATTCTCGAAAGTTTAATTAAAGAACAATACCAGGT
ACTAGGCGTTGTGACCCAGCCAGACCGAAAAGTTGGTCGCAAACACGTCTTAACAGCTTCGCCAGTCAAAGAAGTCGCGG
TTGCCCATGATATTCCCGTCTTCCAACCGGAAAAGATTAGTGGTAGTCCTGAAATGCAACAGATTATTGACTTGCAGCCC
GATTTAATTGTGACGGCCGCCTTTGGACAGTTTTTACCAACAAAATTGTTGAAAGCTGCCAAAATTGGTGCCGTTAATGT
CCATGGCTCGTTGTTGCCTAAGTATCGTGGTGGTGCGCCTGTTCAATATTCAATTATCAACGGTGAGTCTGAAACTGGAA
TTACTATCATTTACATGGTTAAAAAGATGGATGCGGGCGATATGCTGGCGCAGCGTGCGATTCCAATTGAAAAAAACGAT
GACACCGGGACGATGTTTGATAAGTTGAGCTTAGTTGGTCGCGACTTGTTACTGGAAACATTGCCCAAGTTGATTGCTGG
CGAAATTACGGCGGTGCCACAGGATGAATCCCAAGTGACCTTTGCACCGAACATTCAGCCAGAACAAGAGGTTTTAGACT
TCTTTACAAAAACGGCCGTTGAAATTGATAATCAAGTTCGGGGTATGCGGCCAGCACCAATCGCCTATGCGGTCTTGGAT
GGGAAGCGCACGAAGTTCTGGGATGTAACCCCGTTAGCTGACGAAGTGGTCACGCAAGCTCCTGGACAAGTGGTTCGCAA
GACGAAACATGAGCTTGTGATTGCAGCTGCTGACCACACAGCGATTGCAATCAATCAGTTGCAGCCAGCTGGTAAACCGA
AGATGACAATTACAGACTATTTAAATGGGGCGGCAGATAAGTTCGCTACCGGAGAGCAGGTTATTAATAAATGA

Upstream 100 bases:

>100_bases
TACTGCATGAGACGCAGGCGCAACAACGACAAGGGCTACAGATTGGCATTGACGTGGAACCCTTACATTTCATGTAATGG
GATTAGGAGGAAGTTTGACA

Downstream 100 bases:

>100_bases
GTACAGTTGGTAATACCCCCCGCTGGTTAGCGGTCGCCGCCTTAGCGAAGATTAAGAACGGTGCATATTCAAATTTGCAA
TTAAACCAGTTAATCAATGA

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MTSIVFMGTPAFAAPILESLIKEQYQVLGVVTQPDRKVGRKHVLTASPVKEVAVAHDIPVFQPEKISGSPEMQQIIDLQP
DLIVTAAFGQFLPTKLLKAAKIGAVNVHGSLLPKYRGGAPVQYSIINGESETGITIIYMVKKMDAGDMLAQRAIPIEKND
DTGTMFDKLSLVGRDLLLETLPKLIAGEITAVPQDESQVTFAPNIQPEQEVLDFFTKTAVEIDNQVRGMRPAPIAYAVLD
GKRTKFWDVTPLADEVVTQAPGQVVRKTKHELVIAAADHTAIAINQLQPAGKPKMTITDYLNGAADKFATGEQVINK

Sequences:

>Translated_317_residues
MTSIVFMGTPAFAAPILESLIKEQYQVLGVVTQPDRKVGRKHVLTASPVKEVAVAHDIPVFQPEKISGSPEMQQIIDLQP
DLIVTAAFGQFLPTKLLKAAKIGAVNVHGSLLPKYRGGAPVQYSIINGESETGITIIYMVKKMDAGDMLAQRAIPIEKND
DTGTMFDKLSLVGRDLLLETLPKLIAGEITAVPQDESQVTFAPNIQPEQEVLDFFTKTAVEIDNQVRGMRPAPIAYAVLD
GKRTKFWDVTPLADEVVTQAPGQVVRKTKHELVIAAADHTAIAINQLQPAGKPKMTITDYLNGAADKFATGEQVINK
>Mature_316_residues
TSIVFMGTPAFAAPILESLIKEQYQVLGVVTQPDRKVGRKHVLTASPVKEVAVAHDIPVFQPEKISGSPEMQQIIDLQPD
LIVTAAFGQFLPTKLLKAAKIGAVNVHGSLLPKYRGGAPVQYSIINGESETGITIIYMVKKMDAGDMLAQRAIPIEKNDD
TGTMFDKLSLVGRDLLLETLPKLIAGEITAVPQDESQVTFAPNIQPEQEVLDFFTKTAVEIDNQVRGMRPAPIAYAVLDG
KRTKFWDVTPLADEVVTQAPGQVVRKTKHELVIAAADHTAIAINQLQPAGKPKMTITDYLNGAADKFATGEQVINK

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI238814322, Length=327, Percent_Identity=24.4648318042813, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI21614513, Length=318, Percent_Identity=23.2704402515723, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI164663775, Length=316, Percent_Identity=27.2151898734177, Blast_Score=89, Evalue=8e-18,
Organism=Escherichia coli, GI1789683, Length=312, Percent_Identity=37.1794871794872, Blast_Score=222, Evalue=2e-59,
Organism=Escherichia coli, GI1788589, Length=265, Percent_Identity=32.8301886792453, Blast_Score=136, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI133930964, Length=324, Percent_Identity=25.6172839506173, Blast_Score=107, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6319458, Length=280, Percent_Identity=25.3571428571429, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI45550868, Length=303, Percent_Identity=30.03300330033, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI28571984, Length=228, Percent_Identity=31.140350877193, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24585660, Length=332, Percent_Identity=26.5060240963855, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_LACPL (Q88WL3)

Other databases:

- EMBL:   AL935256
- RefSeq:   NP_785208.1
- ProteinModelPortal:   Q88WL3
- SMR:   Q88WL3
- GeneID:   1062180
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_1616
- NMPDR:   fig|220668.1.peg.1342
- HOGENOM:   HBG571560
- OMA:   VVAYGAI
- ProtClustDB:   CLSK581455
- BioCyc:   LPLA220668:LP_1616-MONOMER
- BRENDA:   2.1.2.9
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 34402; Mature: 34271

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSIVFMGTPAFAAPILESLIKEQYQVLGVVTQPDRKVGRKHVLTASPVKEVAVAHDIPV
CCEEEEECCCHHHHHHHHHHHHHHHHHEEEEECCHHHHCHHHEECCCCHHHHHHHHCCCC
FQPEKISGSPEMQQIIDLQPDLIVTAAFGQFLPTKLLKAAKIGAVNVHGSLLPKYRGGAP
CCCCCCCCCHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCC
VQYSIINGESETGITIIYMVKKMDAGDMLAQRAIPIEKNDDTGTMFDKLSLVGRDLLLET
EEEEEECCCCCCCEEEEEEEEECCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LPKLIAGEITAVPQDESQVTFAPNIQPEQEVLDFFTKTAVEIDNQVRGMRPAPIAYAVLD
HHHHHHCCEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
GKRTKFWDVTPLADEVVTQAPGQVVRKTKHELVIAAADHTAIAINQLQPAGKPKMTITDY
CCCCEEEECCCCHHHHHHHCCHHHHHHCCCEEEEEECCCCEEEEECCCCCCCCCEEHHHH
LNGAADKFATGEQVINK
HCCHHHHHCCHHHHHCC
>Mature Secondary Structure 
TSIVFMGTPAFAAPILESLIKEQYQVLGVVTQPDRKVGRKHVLTASPVKEVAVAHDIPV
CEEEEECCCHHHHHHHHHHHHHHHHHEEEEECCHHHHCHHHEECCCCHHHHHHHHCCCC
FQPEKISGSPEMQQIIDLQPDLIVTAAFGQFLPTKLLKAAKIGAVNVHGSLLPKYRGGAP
CCCCCCCCCHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCC
VQYSIINGESETGITIIYMVKKMDAGDMLAQRAIPIEKNDDTGTMFDKLSLVGRDLLLET
EEEEEECCCCCCCEEEEEEEEECCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LPKLIAGEITAVPQDESQVTFAPNIQPEQEVLDFFTKTAVEIDNQVRGMRPAPIAYAVLD
HHHHHHCCEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
GKRTKFWDVTPLADEVVTQAPGQVVRKTKHELVIAAADHTAIAINQLQPAGKPKMTITDY
CCCCEEEECCCCHHHHHHHCCHHHHHHCCCEEEEEECCCCEEEEECCCCCCCCCEEHHHH
LNGAADKFATGEQVINK
HCCHHHHHCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566