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The map label for this gene is pheS

Identifier: 28378265

GI number: 28378265

Start: 1423217

End: 1424263

Strand: Direct

Name: pheS

Synonym: lp_1558

Alternate gene names: 28378265

Gene position: 1423217-1424263 (Clockwise)

Preceding gene: 28378264

Following gene: 28378266

Centisome position: 43.02

GC content: 46.51

Gene sequence:

>1047_bases
ATGAGTTTACAAGATCGATTAACCGAATTACGCGATCAAGGCTTGGCCGATATTAAATCCGCCGATGTTTTGAAAAAGGT
TAACCAAGTCAAAGTTGATTTGCTTGGTAAAAAGGGTCCGATTACAGAAGTATTGCGCGGAATGCGCGACTTAAGCCCGG
AAGAACGGCCAAAGGTGGGCGCTTATGCCAACGAAGTTCGTGACCGGATTCAGGCTGCGATTGATGAACGCCGTGAAGAA
CTGGAACAAGCAGCCGTTAATGAGCAATTGGCTGCCGAAAAACTGGACGTGACGTTACCGGGTCGGGAAGTTCCACAAGG
TCAGCCTCACGTGATTACCCAGATTATTACTGAATTGGAAGATCTATTTATGGGAATGGGCTATCAAATTGTTGATGGTG
ATGAAGTTGAAGAAGATTACTACAACTTTGAACGGTTGAACTTACCGAAGGACCATCCCGCCCGTGACATGCAAGACACG
TTCTATATTACCAAAGACGTGCTACTACGCACGCAGACGTCTGCTGATCAGCCGCGGTCACTTGAAAATCACGATTTTTC
TAAAGGACCGCTGAAGGTCTTGTCACCTGGCCGCGTTTATCGGCGTGATACGGATGATGCAACCCATTCCCATCAATTTC
ATCAAATTGAAGGGTTAGTCGTGGACAAGCATATTACGATGGCTGATTTGAAGGGCACCTTAATTCTGGTTGCCAAGACT
TTGTTTGGCGATCAATTCGATGTTCGGCTACGGCCAAGCTTCTTTCCATTCACGGAACCATCCGTAGAAGCTGATGTAAC
TTGCTTTAATTGCAATGGCAAGGGCTGTGCAATCTGTAAGCAAACGGGTTGGATCGAAGTACTGGGTGCCGGCATGGTTC
ACCCCCACGTGTTAGAAATGTCTGGCATTGATCCAGAAGAATATGGTGGTTTTGCCTTTGGGTTAGGACCAGACCGCTTT
GCAATGTTGAAATACGGTGTTGACGATATCCGCAACTTCTACTTGAATGACGTGCGGTTCTTGTCACAGTTCTATAAGAA
AGGTTAG

Upstream 100 bases:

>100_bases
GACTACTATGCAAACAAGGGTGGTACCGCGATGACCTCGTCCCTTTTCAGGGATGGGGTCTTTTTTTGTCCGCAACGCAA
CTAAAAATTGGAGGCAGCAT

Downstream 100 bases:

>100_bases
GCGTCCACATGAGAATTTCTACGACATGGTTACGCGATTATTTGAAGCTGGACATTCCGGCCGATGAATTAGCAGAAAAG
ATTGAACGGACGGCCGTTGA

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MSLQDRLTELRDQGLADIKSADVLKKVNQVKVDLLGKKGPITEVLRGMRDLSPEERPKVGAYANEVRDRIQAAIDERREE
LEQAAVNEQLAAEKLDVTLPGREVPQGQPHVITQIITELEDLFMGMGYQIVDGDEVEEDYYNFERLNLPKDHPARDMQDT
FYITKDVLLRTQTSADQPRSLENHDFSKGPLKVLSPGRVYRRDTDDATHSHQFHQIEGLVVDKHITMADLKGTLILVAKT
LFGDQFDVRLRPSFFPFTEPSVEADVTCFNCNGKGCAICKQTGWIEVLGAGMVHPHVLEMSGIDPEEYGGFAFGLGPDRF
AMLKYGVDDIRNFYLNDVRFLSQFYKKG

Sequences:

>Translated_348_residues
MSLQDRLTELRDQGLADIKSADVLKKVNQVKVDLLGKKGPITEVLRGMRDLSPEERPKVGAYANEVRDRIQAAIDERREE
LEQAAVNEQLAAEKLDVTLPGREVPQGQPHVITQIITELEDLFMGMGYQIVDGDEVEEDYYNFERLNLPKDHPARDMQDT
FYITKDVLLRTQTSADQPRSLENHDFSKGPLKVLSPGRVYRRDTDDATHSHQFHQIEGLVVDKHITMADLKGTLILVAKT
LFGDQFDVRLRPSFFPFTEPSVEADVTCFNCNGKGCAICKQTGWIEVLGAGMVHPHVLEMSGIDPEEYGGFAFGLGPDRF
AMLKYGVDDIRNFYLNDVRFLSQFYKKG
>Mature_347_residues
SLQDRLTELRDQGLADIKSADVLKKVNQVKVDLLGKKGPITEVLRGMRDLSPEERPKVGAYANEVRDRIQAAIDERREEL
EQAAVNEQLAAEKLDVTLPGREVPQGQPHVITQIITELEDLFMGMGYQIVDGDEVEEDYYNFERLNLPKDHPARDMQDTF
YITKDVLLRTQTSADQPRSLENHDFSKGPLKVLSPGRVYRRDTDDATHSHQFHQIEGLVVDKHITMADLKGTLILVAKTL
FGDQFDVRLRPSFFPFTEPSVEADVTCFNCNGKGCAICKQTGWIEVLGAGMVHPHVLEMSGIDPEEYGGFAFGLGPDRFA
MLKYGVDDIRNFYLNDVRFLSQFYKKG

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=240, Percent_Identity=33.3333333333333, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI4758340, Length=271, Percent_Identity=29.8892988929889, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1788007, Length=338, Percent_Identity=47.3372781065089, Blast_Score=321, Evalue=5e-89,
Organism=Caenorhabditis elegans, GI32563657, Length=271, Percent_Identity=28.4132841328413, Blast_Score=117, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17508957, Length=271, Percent_Identity=28.4132841328413, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI32566635, Length=250, Percent_Identity=34, Blast_Score=111, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6321087, Length=281, Percent_Identity=29.1814946619217, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6325304, Length=161, Percent_Identity=32.9192546583851, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI18858079, Length=271, Percent_Identity=30.6273062730627, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI17137424, Length=254, Percent_Identity=31.496062992126, Blast_Score=108, Evalue=8e-24,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_LACPL (Q88WR3)

Other databases:

- EMBL:   AL935256
- RefSeq:   NP_785157.1
- ProteinModelPortal:   Q88WR3
- SMR:   Q88WR3
- GeneID:   1064071
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_1558
- NMPDR:   fig|220668.1.peg.1291
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- BioCyc:   LPLA220668:LP_1558-MONOMER
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 39364; Mature: 39233

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLQDRLTELRDQGLADIKSADVLKKVNQVKVDLLGKKGPITEVLRGMRDLSPEERPKVG
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCC
AYANEVRDRIQAAIDERREELEQAAVNEQLAAEKLDVTLPGREVPQGQPHVITQIITELE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCHHHHHHHHHHH
DLFMGMGYQIVDGDEVEEDYYNFERLNLPKDHPARDMQDTFYITKDVLLRTQTSADQPRS
HHHHCCCCEEECCCCHHHHHHCHHHCCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCCC
LENHDFSKGPLKVLSPGRVYRRDTDDATHSHQFHQIEGLVVDKHITMADLKGTLILVAKT
CCCCCCCCCCEEEECCCCEEECCCCCCHHCCHHHHHCCEEEECCEEHHHCCCHHEEEEHH
LFGDQFDVRLRPSFFPFTEPSVEADVTCFNCNGKGCAICKQTGWIEVLGAGMVHPHVLEM
HCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCEEECCCCCCCEEEEE
SGIDPEEYGGFAFGLGPDRFAMLKYGVDDIRNFYLNDVRFLSQFYKKG
CCCCHHHHCCEEECCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLQDRLTELRDQGLADIKSADVLKKVNQVKVDLLGKKGPITEVLRGMRDLSPEERPKVG
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCC
AYANEVRDRIQAAIDERREELEQAAVNEQLAAEKLDVTLPGREVPQGQPHVITQIITELE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCHHHHHHHHHHH
DLFMGMGYQIVDGDEVEEDYYNFERLNLPKDHPARDMQDTFYITKDVLLRTQTSADQPRS
HHHHCCCCEEECCCCHHHHHHCHHHCCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCCC
LENHDFSKGPLKVLSPGRVYRRDTDDATHSHQFHQIEGLVVDKHITMADLKGTLILVAKT
CCCCCCCCCCEEEECCCCEEECCCCCCHHCCHHHHHCCEEEECCEEHHHCCCHHEEEEHH
LFGDQFDVRLRPSFFPFTEPSVEADVTCFNCNGKGCAICKQTGWIEVLGAGMVHPHVLEM
HCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCEEECCCCCCCEEEEE
SGIDPEEYGGFAFGLGPDRFAMLKYGVDDIRNFYLNDVRFLSQFYKKG
CCCCHHHHCCEEECCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566