Click here to switch to the map view.

The map label for this gene is pheT

Identifier: 28378266

GI number: 28378266

Start: 1424272

End: 1426689

Strand: Direct

Name: pheT

Synonym: lp_1559

Alternate gene names: 28378266

Gene position: 1424272-1426689 (Clockwise)

Preceding gene: 28378265

Following gene: 28378267

Centisome position: 43.05

GC content: 47.06

Gene sequence:

>2418_bases
ATGAGAATTTCTACGACATGGTTACGCGATTATTTGAAGCTGGACATTCCGGCCGATGAATTAGCAGAAAAGATTGAACG
GACGGCCGTTGAAGTTGACGGTGTTATTCGTCCCAGTGAAGGATTGAAAAAAGTTGTAGTGGGGCACGTCTTAACGTGTG
AGCCACATCCAGATTCTGATCATCTCCACGTTTGTCAAGTGGACGTAGGCGAATCAGAACCCTTGCAAATCGTTTGTGGG
GCACCTAACGTTGCGGCTGGCGAAAAGGTGATCGTGGCGTTACCGAATTCATGGATTGGTGGTCACACTAAGATCAAGAA
GAGTAAGATGCGGGGAGTTCCATCAAATGGGATGCTGTGCGCCTTAGACGAACTTGGTTTTGATGAAAAGTTAGTTCCAA
AAGAAGTTGCGGATGGTATTTTCATTCTGCCTGACGATGCCACGCCAGGTGATCCGGTCTTTTCATACCTAGGGATGGAT
GATGAAATTATTGACATGTCCGTTACCCCTAACCGTGGTGACATGCTTAGTATGAATGGTACGGCGCATGAACTTGCTGC
CATTTACGACCAACAACCAACGATGCCGAAAGTTGATTTGTATGAAGATGCTACGACGGTGGCTGCGGATGATCTACATG
TGGCTGTCGCTGCCGACGAACATGATGTCCCAATGTACAAAATGCGTCTAATTAAAAACGTTACGATTAAGCCAAGTCCG
TTATGGCTCCAAATTCGAATTTGGAATGCTGGTATGCGGCCAATTAATAACGTCGTTGACGCAACAAACTACATTTTGAT
GCAATATGGACAACCGCTACATGCTTTTGACTTTGATCAATTGAATGACGGTCAAATTAACGTCCGGTTGGCGAAAGCTG
GCGAGCATTTAACGACGTTAGATGGTGAAGATCGCGAATTACTAACGAGTGATCTGTTGATTTGTAGTGGCGATCAACCA
ATTTGTTTGGCTGGGACGATGGGTGGCCTAGCGACCGAAGTCACTGATCAGACTACGACCATTGCGTTGGAAGGTGCGGT
TTTTGATGCAGTTAAGATTCGTAAAACTGCCCATAATCATGACTTACACAGCGAAGCTTCAATGCGCTACGAACGGGGCA
TTGACCACGGCATGACCGCAACGGCCTTAGACGCTGCCGCAGCCATGATTGCAGAACTTGGTGATGGTCAAGTTGCTAGT
GGTATGGTCATCGGTCGTGACGAGGATGTGCAACCAACGACAGTAACCATTGACTTAGCACGGATCAACCACGTCTTAGG
AACTGAGTTGTCATTGGACACGGTCAGTGATATTTTTAAACGATTGGACTTTCCAACGGTCGTTGCTAATGAAACGTTCA
CGGTTACGGTGCCATCACGGCGTTGGGATATCCATATTCCGGCTGACCTTATCGAAGAAATCGCCCGGCTATACGGTTAT
GATAACTTACCAGCGACCCTACCAACGGGCCAACCAACAATCGGTAAGCTTAATGAGACCCAACAGGTGATTCGTGACTC
ACGTAAGTTAATGGAAAGTGCTGGCCTCACGCAAGCCATCAGTTACTCATTGACCACGGAGACTAAGTCGAAGGCCTTTG
CGCTTCATGCTAGTGACGTCACTAAATTAGACTTTCCAATGAGTTCAGAACGGACCACGCTACGCTTGAGTCTAGTTTCT
GGTTTGCTGGATGATTTAGCTTATAACAATGCTCGTAAGGAACACAATGTGGCATTGTATGAAGAAGGACGGGTCTTTTA
CAGTCAACCTGAACAAGTCCGGCCGAAGGAAATTGAACACATTGCCGGAGCTATTACGGGTTCCATGGTTCCCAAGAGCT
GGGGCGTTGCCGAACAACCCGTTGATTTCTTCCAGATTAAGGGTATCGTGGCTGGTTATCTGAAGAGTCTGGCGTTGCAA
GATGCCGTTAGTTACGTTGCCACGGCCGAACATCCAGAGATGCATCCTGGTCGGACAGCTGATATTTACGTTGGTGACCA
GTTAGTCGGTTTCGTCGGTGAAGTTCATCCAACGACTGCCAAAGCCTACAAGATTCGGGAAACTTATGTCTTTGAACTGG
ATTTAACGGCGTTGATTGCCTTACCAAAAGCGCGTCAACAGTATCAACCGATTTCCAAGTTCCCAAGTATCACCCGGGAC
GTGGCGATGTTGATTGATGACGATGTGACCAACGCAACCGTGGTTGCCTTGATCAACAAGAAGGGCGGCGCGCATTTACG
GCACGTTCAACTCTTTGATGTTTATAATGGGAGTCACGTGCCAGCGGGTAAAAAGTCGCTAGCTTATACGTTAACGTATC
AAGACCAAAACGCAACGTTAGTCGATGATGATGTTACGACAGCATTTGAAAAAGTACTGACAGCCTTAACGGATGAATTA
GGTGCTGAAATTCGTTAG

Upstream 100 bases:

>100_bases
GCTTTGCAATGTTGAAATACGGTGTTGACGATATCCGCAACTTCTACTTGAATGACGTGCGGTTCTTGTCACAGTTCTAT
AAGAAAGGTTAGGCGTCCAC

Downstream 100 bases:

>100_bases
TTTGTTGATAGTCTTGTTAAACGCCGAATGATTTGAAGTCATTCGGCGTTTAATGTGTGTCCAAGATCCTAAAAAACCAT
AAAACTAATGGCGAACTAGT

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS

Number of amino acids: Translated: 805; Mature: 805

Protein sequence:

>805_residues
MRISTTWLRDYLKLDIPADELAEKIERTAVEVDGVIRPSEGLKKVVVGHVLTCEPHPDSDHLHVCQVDVGESEPLQIVCG
APNVAAGEKVIVALPNSWIGGHTKIKKSKMRGVPSNGMLCALDELGFDEKLVPKEVADGIFILPDDATPGDPVFSYLGMD
DEIIDMSVTPNRGDMLSMNGTAHELAAIYDQQPTMPKVDLYEDATTVAADDLHVAVAADEHDVPMYKMRLIKNVTIKPSP
LWLQIRIWNAGMRPINNVVDATNYILMQYGQPLHAFDFDQLNDGQINVRLAKAGEHLTTLDGEDRELLTSDLLICSGDQP
ICLAGTMGGLATEVTDQTTTIALEGAVFDAVKIRKTAHNHDLHSEASMRYERGIDHGMTATALDAAAAMIAELGDGQVAS
GMVIGRDEDVQPTTVTIDLARINHVLGTELSLDTVSDIFKRLDFPTVVANETFTVTVPSRRWDIHIPADLIEEIARLYGY
DNLPATLPTGQPTIGKLNETQQVIRDSRKLMESAGLTQAISYSLTTETKSKAFALHASDVTKLDFPMSSERTTLRLSLVS
GLLDDLAYNNARKEHNVALYEEGRVFYSQPEQVRPKEIEHIAGAITGSMVPKSWGVAEQPVDFFQIKGIVAGYLKSLALQ
DAVSYVATAEHPEMHPGRTADIYVGDQLVGFVGEVHPTTAKAYKIRETYVFELDLTALIALPKARQQYQPISKFPSITRD
VAMLIDDDVTNATVVALINKKGGAHLRHVQLFDVYNGSHVPAGKKSLAYTLTYQDQNATLVDDDVTTAFEKVLTALTDEL
GAEIR

Sequences:

>Translated_805_residues
MRISTTWLRDYLKLDIPADELAEKIERTAVEVDGVIRPSEGLKKVVVGHVLTCEPHPDSDHLHVCQVDVGESEPLQIVCG
APNVAAGEKVIVALPNSWIGGHTKIKKSKMRGVPSNGMLCALDELGFDEKLVPKEVADGIFILPDDATPGDPVFSYLGMD
DEIIDMSVTPNRGDMLSMNGTAHELAAIYDQQPTMPKVDLYEDATTVAADDLHVAVAADEHDVPMYKMRLIKNVTIKPSP
LWLQIRIWNAGMRPINNVVDATNYILMQYGQPLHAFDFDQLNDGQINVRLAKAGEHLTTLDGEDRELLTSDLLICSGDQP
ICLAGTMGGLATEVTDQTTTIALEGAVFDAVKIRKTAHNHDLHSEASMRYERGIDHGMTATALDAAAAMIAELGDGQVAS
GMVIGRDEDVQPTTVTIDLARINHVLGTELSLDTVSDIFKRLDFPTVVANETFTVTVPSRRWDIHIPADLIEEIARLYGY
DNLPATLPTGQPTIGKLNETQQVIRDSRKLMESAGLTQAISYSLTTETKSKAFALHASDVTKLDFPMSSERTTLRLSLVS
GLLDDLAYNNARKEHNVALYEEGRVFYSQPEQVRPKEIEHIAGAITGSMVPKSWGVAEQPVDFFQIKGIVAGYLKSLALQ
DAVSYVATAEHPEMHPGRTADIYVGDQLVGFVGEVHPTTAKAYKIRETYVFELDLTALIALPKARQQYQPISKFPSITRD
VAMLIDDDVTNATVVALINKKGGAHLRHVQLFDVYNGSHVPAGKKSLAYTLTYQDQNATLVDDDVTTAFEKVLTALTDEL
GAEIR
>Mature_805_residues
MRISTTWLRDYLKLDIPADELAEKIERTAVEVDGVIRPSEGLKKVVVGHVLTCEPHPDSDHLHVCQVDVGESEPLQIVCG
APNVAAGEKVIVALPNSWIGGHTKIKKSKMRGVPSNGMLCALDELGFDEKLVPKEVADGIFILPDDATPGDPVFSYLGMD
DEIIDMSVTPNRGDMLSMNGTAHELAAIYDQQPTMPKVDLYEDATTVAADDLHVAVAADEHDVPMYKMRLIKNVTIKPSP
LWLQIRIWNAGMRPINNVVDATNYILMQYGQPLHAFDFDQLNDGQINVRLAKAGEHLTTLDGEDRELLTSDLLICSGDQP
ICLAGTMGGLATEVTDQTTTIALEGAVFDAVKIRKTAHNHDLHSEASMRYERGIDHGMTATALDAAAAMIAELGDGQVAS
GMVIGRDEDVQPTTVTIDLARINHVLGTELSLDTVSDIFKRLDFPTVVANETFTVTVPSRRWDIHIPADLIEEIARLYGY
DNLPATLPTGQPTIGKLNETQQVIRDSRKLMESAGLTQAISYSLTTETKSKAFALHASDVTKLDFPMSSERTTLRLSLVS
GLLDDLAYNNARKEHNVALYEEGRVFYSQPEQVRPKEIEHIAGAITGSMVPKSWGVAEQPVDFFQIKGIVAGYLKSLALQ
DAVSYVATAEHPEMHPGRTADIYVGDQLVGFVGEVHPTTAKAYKIRETYVFELDLTALIALPKARQQYQPISKFPSITRD
VAMLIDDDVTNATVVALINKKGGAHLRHVQLFDVYNGSHVPAGKKSLAYTLTYQDQNATLVDDDVTTAFEKVLTALTDEL
GAEIR

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Escherichia coli, GI1788006, Length=813, Percent_Identity=34.8093480934809, Blast_Score=455, Evalue=1e-129,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFB_LACPL (Q88WR2)

Other databases:

- EMBL:   AL935256
- RefSeq:   NP_785158.1
- ProteinModelPortal:   Q88WR2
- SMR:   Q88WR2
- GeneID:   1064070
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_1559
- NMPDR:   fig|220668.1.peg.1292
- HOGENOM:   HBG399638
- OMA:   ADKLRVC
- ProtClustDB:   PRK00629
- BioCyc:   LPLA220668:LP_1559-MONOMER
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00283
- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547
- Gene3D:   G3DSA:3.50.40.10
- Gene3D:   G3DSA:3.30.56.20
- Gene3D:   G3DSA:3.30.70.380
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00873
- SMART:   SM00874
- SMART:   SM00896
- TIGRFAMs:   TIGR00472

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind; SSF56037 B3_4; SSF54991 Fdx_AntiC_bd; SSF50249 Nucleic_acid_OB; SSF46955 Putativ_DNA_bind

EC number: =6.1.1.20

Molecular weight: Translated: 88120; Mature: 88120

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS51483 B5; PS51447 FDX_ACB; PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRISTTWLRDYLKLDIPADELAEKIERTAVEVDGVIRPSEGLKKVVVGHVLTCEPHPDSD
CCCCHHHHHHHHEECCCHHHHHHHHHHHHHHCCCEECCHHHHHHHHHCEEEEECCCCCCC
HLHVCQVDVGESEPLQIVCGAPNVAAGEKVIVALPNSWIGGHTKIKKSKMRGVPSNGMLC
CEEEEEEECCCCCCEEEEECCCCCCCCCEEEEECCCCCCCCCCEEEHHHHCCCCCCCCEE
ALDELGFDEKLVPKEVADGIFILPDDATPGDPVFSYLGMDDEIIDMSVTPNRGDMLSMNG
EEHHCCCCCCCCCHHHCCCEEEEECCCCCCCHHHHHCCCCCCEEEEEECCCCCCEEEECC
TAHELAAIYDQQPTMPKVDLYEDATTVAADDLHVAVAADEHDVPMYKMRLIKNVTIKPSP
CHHHHHHHHCCCCCCCCCEECCCCCEEEECCEEEEEECCCCCCCHHHHHHHHCCEECCCC
LWLQIRIWNAGMRPINNVVDATNYILMQYGQPLHAFDFDQLNDGQINVRLAKAGEHLTTL
EEEEEEEECCCCCHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCEEEC
DGEDRELLTSDLLICSGDQPICLAGTMGGLATEVTDQTTTIALEGAVFDAVKIRKTAHNH
CCCCHHHHHCCEEEECCCCCEEEEECCCCCEEECCCCEEEEEECCCHHHHHHHHHHHCCC
DLHSEASMRYERGIDHGMTATALDAAAAMIAELGDGQVASGMVIGRDEDVQPTTVTIDLA
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEECCEEECCCCCCCCEEEEEEHH
RINHVLGTELSLDTVSDIFKRLDFPTVVANETFTVTVPSRRWDIHIPADLIEEIARLYGY
HHHHHHCCCCCHHHHHHHHHHCCCCEEEECCEEEEEECCCEEEEECCHHHHHHHHHHCCC
DNLPATLPTGQPTIGKLNETQQVIRDSRKLMESAGLTQAISYSLTTETKSKAFALHASDV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCCCEEEEECCCC
TKLDFPMSSERTTLRLSLVSGLLDDLAYNNARKEHNVALYEEGRVFYSQPEQVRPKEIEH
EEECCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCEEEEECCEEEECCCCCCCHHHHHH
IAGAITGSMVPKSWGVAEQPVDFFQIKGIVAGYLKSLALQDAVSYVATAEHPEMHPGRTA
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
DIYVGDQLVGFVGEVHPTTAKAYKIRETYVFELDLTALIALPKARQQYQPISKFPSITRD
EEEECCHHHHHHCCCCCCCHHEEEEEEEEEEEEEHHHHHCCCHHHHHCCCHHHCCCHHHH
VAMLIDDDVTNATVVALINKKGGAHLRHVQLFDVYNGSHVPAGKKSLAYTLTYQDQNATL
HHHEECCCCCCEEEEEEEECCCCCEEEEEEEEEEECCCCCCCCCCCEEEEEEEECCCCEE
VDDDVTTAFEKVLTALTDELGAEIR
ECCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRISTTWLRDYLKLDIPADELAEKIERTAVEVDGVIRPSEGLKKVVVGHVLTCEPHPDSD
CCCCHHHHHHHHEECCCHHHHHHHHHHHHHHCCCEECCHHHHHHHHHCEEEEECCCCCCC
HLHVCQVDVGESEPLQIVCGAPNVAAGEKVIVALPNSWIGGHTKIKKSKMRGVPSNGMLC
CEEEEEEECCCCCCEEEEECCCCCCCCCEEEEECCCCCCCCCCEEEHHHHCCCCCCCCEE
ALDELGFDEKLVPKEVADGIFILPDDATPGDPVFSYLGMDDEIIDMSVTPNRGDMLSMNG
EEHHCCCCCCCCCHHHCCCEEEEECCCCCCCHHHHHCCCCCCEEEEEECCCCCCEEEECC
TAHELAAIYDQQPTMPKVDLYEDATTVAADDLHVAVAADEHDVPMYKMRLIKNVTIKPSP
CHHHHHHHHCCCCCCCCCEECCCCCEEEECCEEEEEECCCCCCCHHHHHHHHCCEECCCC
LWLQIRIWNAGMRPINNVVDATNYILMQYGQPLHAFDFDQLNDGQINVRLAKAGEHLTTL
EEEEEEEECCCCCHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCEEEC
DGEDRELLTSDLLICSGDQPICLAGTMGGLATEVTDQTTTIALEGAVFDAVKIRKTAHNH
CCCCHHHHHCCEEEECCCCCEEEEECCCCCEEECCCCEEEEEECCCHHHHHHHHHHHCCC
DLHSEASMRYERGIDHGMTATALDAAAAMIAELGDGQVASGMVIGRDEDVQPTTVTIDLA
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEECCEEECCCCCCCCEEEEEEHH
RINHVLGTELSLDTVSDIFKRLDFPTVVANETFTVTVPSRRWDIHIPADLIEEIARLYGY
HHHHHHCCCCCHHHHHHHHHHCCCCEEEECCEEEEEECCCEEEEECCHHHHHHHHHHCCC
DNLPATLPTGQPTIGKLNETQQVIRDSRKLMESAGLTQAISYSLTTETKSKAFALHASDV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCCCEEEEECCCC
TKLDFPMSSERTTLRLSLVSGLLDDLAYNNARKEHNVALYEEGRVFYSQPEQVRPKEIEH
EEECCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCEEEEECCEEEECCCCCCCHHHHHH
IAGAITGSMVPKSWGVAEQPVDFFQIKGIVAGYLKSLALQDAVSYVATAEHPEMHPGRTA
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
DIYVGDQLVGFVGEVHPTTAKAYKIRETYVFELDLTALIALPKARQQYQPISKFPSITRD
EEEECCHHHHHHCCCCCCCHHEEEEEEEEEEEEEHHHHHCCCHHHHHCCCHHHCCCHHHH
VAMLIDDDVTNATVVALINKKGGAHLRHVQLFDVYNGSHVPAGKKSLAYTLTYQDQNATL
HHHEECCCCCCEEEEEEEECCCCCEEEEEEEEEEECCCCCCCCCCCEEEEEEEECCCCEE
VDDDVTTAFEKVLTALTDELGAEIR
ECCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566