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The map label for this gene is polA [H]

Identifier: 28378222

GI number: 28378222

Start: 1378501

End: 1381149

Strand: Direct

Name: polA [H]

Synonym: lp_1508

Alternate gene names: 28378222

Gene position: 1378501-1381149 (Clockwise)

Preceding gene: 28378221

Following gene: 28378223

Centisome position: 41.67

GC content: 45.19

Gene sequence:

>2649_bases
ATGGCAAAAAAATTATTACTGATTGATGGTAACAGTGTGGCATTTCGGGCGTTCTTTGCGTTGCATAGTCAACTGGAACG
GTTCGTAAATCCAGACGGCCTGCATACCAACGCCATTTATGGCTTCAATACAATGCTTGACCACATGATCAAGGCAGTCG
AACCCACGGATATGTTAGTGGCTTTTGATGCGGGTAAGACCACGTTTCGAACAGCCATGTACGATAATTACAAGGGTGGC
CGGGCTAAGACGCCTAGTGAGTTTTCTGAACAAATGCCGTATATCAAACAGCTATTAGACGCGTATGGTATCAAACATTA
TGAATTAAAAGACTACGAAGCCGATGACATTATTGGCACACTGGCCGCTCAAGCGGATCAAACCGGCTACACCACGACGG
TCGTCACTGGTGACCGTGATTTGACGCAGTTGACGACCGAGCATACCACGGTGGCCGTGACCGTTAAAGGTGTTTCTGAA
GTGGAACACTATACGCCGGCGCACGTTGAAGAAAAGCTGGGCATCACACCGGCACAAATTATTGACATGAAGGGGTTGAC
AGGTGATACCTCGGATAACTATCCGGGTGTCACCAAGGTTGGTGAGAAGACCGCCCTTAAATTACTCAAACAGTATGGTA
CGATGGAAGGCATTTATGAAAACATCGATGCCATGAAACAGAGCAAGATGAAGGAACATCTGATTGAGGATCGCGAACAA
GCGGCGCAGGCTAAGGTGCTAGCAACAATCAAGCGGGATGCGCCGATTGATGTTCAATTGGCTGATTTATCCTATGATGG
CCCGGATCTTGACCAGCTGGCCGCGTTTTATCAGAAGATGGACTTTCAATCATTTTTGAAGAAATTACACCTACAGTCGG
ACGAACCAGCAACGCCCATTGATTATACCGTCTTGACGAAAGCTAACCTGACGGAATTGCAACAATTTACCGATCACGTG
GACTTTTATTTGGAGATGCCAACGCCGAACTACCACACGTCGGCCTTTGCTGGCTTTGCCATCGGAGCGGATGATCACTG
GTACATCAGTCGGGATGTGGAATTATTAATGCAACCGACTGTGGCTGATCTCTTGAGTAGCACAACGATTACCAAGGACG
TTTTTGATATGAAACGCACGACGGTCGGGCTTAATCGTTTGGGTATTCAGCTAGGTAAAGTCGATTTTGATCTTTTACTC
GTTTCATACTTATTAGATACAAATGATAACAGTAACGACTTGGGAAGTTTAGCTGAGCAACACGGGTACACTGACTTGCC
GAGTGACGAGACCGTTTATGGTAAGGGCGTTAAGCGCGCTATTCCTGAAGATGATGACGTCTTCTTTAGTCATCTCGCCC
GGAAATTGGCCGCAATCGCTAGTTTGAAGCCACAGTTATTAAAGGAATTGGCAGCCAATGAGCAGGATCAACTTTACGAT
GATATCGAAAAACCGATGGCTAATGTCTTAGCTCAGATGGAAATCGCCGGCATTACGGTCGACAGCAGTCGACTTAAAGC
CATGGGCAGTCAGTTTACAGAACGGTTGAGTGAAATTGAACAGACGATTTATCAAGAAGCCGGTGAAGAGTTCAATATTA
ATTCACCTAAGCAACTAGGTCACATCTTGTTTGAAGAAATGAAATTACCCGTAATCAAGAAGACCAAGACAGGGTATTCT
ACCGCCGTAGACGTTCTAGAAAAGTTAGCACCGGAAGCACCAATCGTCGCTAATATTTTACAATATCGTCAGATCTCGAA
GATTCAATCGACCTACGTTGTCGGTTTGTTAGATGCCATTCATTCTGCTGATCAAAAGGTGCATACGCGCTACTTGCAAA
CGTTGACCCAAACCGGCCGGTTGTCGTCCGTTGATCCAAACTTACAAAACATTCCGGTGCGCTTGGAAGAAGGACGTAAG
ATTCGACAAGCCTTTGTTCCTAGTCATGAAGGTTGGCAGATTTTCTCGTCTGATTATTCGCAAATCGAATTACGGGTTCT
CGCGCATATTACTGATGATGAAAATATGCAGGCCGCTTTTAAAGCGGGTGATGATATTCATGCCGCAACGGCCATGCGAA
TCTTTCATTTGAACTCAGCTGAAGAGGTGACGCCAAATATTCGACGGCAAGCTAAGGCAGTTAACTTTGGAATCGTCTAT
GGAATTAGTGATTACGGCTTGTCACAAAATATCGGCATTACGCGTAAACAGGCTCGCAGCTTTATTGACGCTTACTTCAA
AGAATATCCGGGTGTCAAAAAGTATATGGATGAAATTGTCCAAACGGCGAAAAAACAAGGTTATGTGGAAACCATTTCAC
ATCGACGCCGGTACTTGCCAGATATTAATTCCAAGAGCTTCAACCAACGCTCATTCGCTGAACGGACAGCCATGAATACC
CCCATCCAAGGGAGTGCGGCTGATATTATCAAGATTGCGATGATTAAGATGCAAGATAAGTTACAGGCTGCGGGTTTGAA
AGCAACCATGCTGTTACAAGTTCATGATGAATTGATTTTTGAAGCGCCAAAAGAAGAAATTCCAGTTTTAGAAAAATTAG
TGCCAAGCGTGATGGATTCCGCCGTACAGTTAGCCGTACCGTTAAAGGTTGAGAGCCACTACGGTGATACTTGGTACGAT
GCAAAATAG

Upstream 100 bases:

>100_bases
AGGCGCTGCGAATCAGACCGCCAATCGAGTGGTATTAATGGTCTGAGTGACGGATTTTCATAAAAATGATGATTTAAATT
AAGAGATGGGGAGCAAACTA

Downstream 100 bases:

>100_bases
GGGGAACCACCATGCCTGAATTACCTGAAGTTGAAACTGTTCGTCGCGGCTTAAATCGTCTGGTTAGCGGGGCAACGATT
GCTAGTATCGAGGTTTTTTG

Product: DNA-directed DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 882; Mature: 881

Protein sequence:

>882_residues
MAKKLLLIDGNSVAFRAFFALHSQLERFVNPDGLHTNAIYGFNTMLDHMIKAVEPTDMLVAFDAGKTTFRTAMYDNYKGG
RAKTPSEFSEQMPYIKQLLDAYGIKHYELKDYEADDIIGTLAAQADQTGYTTTVVTGDRDLTQLTTEHTTVAVTVKGVSE
VEHYTPAHVEEKLGITPAQIIDMKGLTGDTSDNYPGVTKVGEKTALKLLKQYGTMEGIYENIDAMKQSKMKEHLIEDREQ
AAQAKVLATIKRDAPIDVQLADLSYDGPDLDQLAAFYQKMDFQSFLKKLHLQSDEPATPIDYTVLTKANLTELQQFTDHV
DFYLEMPTPNYHTSAFAGFAIGADDHWYISRDVELLMQPTVADLLSSTTITKDVFDMKRTTVGLNRLGIQLGKVDFDLLL
VSYLLDTNDNSNDLGSLAEQHGYTDLPSDETVYGKGVKRAIPEDDDVFFSHLARKLAAIASLKPQLLKELAANEQDQLYD
DIEKPMANVLAQMEIAGITVDSSRLKAMGSQFTERLSEIEQTIYQEAGEEFNINSPKQLGHILFEEMKLPVIKKTKTGYS
TAVDVLEKLAPEAPIVANILQYRQISKIQSTYVVGLLDAIHSADQKVHTRYLQTLTQTGRLSSVDPNLQNIPVRLEEGRK
IRQAFVPSHEGWQIFSSDYSQIELRVLAHITDDENMQAAFKAGDDIHAATAMRIFHLNSAEEVTPNIRRQAKAVNFGIVY
GISDYGLSQNIGITRKQARSFIDAYFKEYPGVKKYMDEIVQTAKKQGYVETISHRRRYLPDINSKSFNQRSFAERTAMNT
PIQGSAADIIKIAMIKMQDKLQAAGLKATMLLQVHDELIFEAPKEEIPVLEKLVPSVMDSAVQLAVPLKVESHYGDTWYD
AK

Sequences:

>Translated_882_residues
MAKKLLLIDGNSVAFRAFFALHSQLERFVNPDGLHTNAIYGFNTMLDHMIKAVEPTDMLVAFDAGKTTFRTAMYDNYKGG
RAKTPSEFSEQMPYIKQLLDAYGIKHYELKDYEADDIIGTLAAQADQTGYTTTVVTGDRDLTQLTTEHTTVAVTVKGVSE
VEHYTPAHVEEKLGITPAQIIDMKGLTGDTSDNYPGVTKVGEKTALKLLKQYGTMEGIYENIDAMKQSKMKEHLIEDREQ
AAQAKVLATIKRDAPIDVQLADLSYDGPDLDQLAAFYQKMDFQSFLKKLHLQSDEPATPIDYTVLTKANLTELQQFTDHV
DFYLEMPTPNYHTSAFAGFAIGADDHWYISRDVELLMQPTVADLLSSTTITKDVFDMKRTTVGLNRLGIQLGKVDFDLLL
VSYLLDTNDNSNDLGSLAEQHGYTDLPSDETVYGKGVKRAIPEDDDVFFSHLARKLAAIASLKPQLLKELAANEQDQLYD
DIEKPMANVLAQMEIAGITVDSSRLKAMGSQFTERLSEIEQTIYQEAGEEFNINSPKQLGHILFEEMKLPVIKKTKTGYS
TAVDVLEKLAPEAPIVANILQYRQISKIQSTYVVGLLDAIHSADQKVHTRYLQTLTQTGRLSSVDPNLQNIPVRLEEGRK
IRQAFVPSHEGWQIFSSDYSQIELRVLAHITDDENMQAAFKAGDDIHAATAMRIFHLNSAEEVTPNIRRQAKAVNFGIVY
GISDYGLSQNIGITRKQARSFIDAYFKEYPGVKKYMDEIVQTAKKQGYVETISHRRRYLPDINSKSFNQRSFAERTAMNT
PIQGSAADIIKIAMIKMQDKLQAAGLKATMLLQVHDELIFEAPKEEIPVLEKLVPSVMDSAVQLAVPLKVESHYGDTWYD
AK
>Mature_881_residues
AKKLLLIDGNSVAFRAFFALHSQLERFVNPDGLHTNAIYGFNTMLDHMIKAVEPTDMLVAFDAGKTTFRTAMYDNYKGGR
AKTPSEFSEQMPYIKQLLDAYGIKHYELKDYEADDIIGTLAAQADQTGYTTTVVTGDRDLTQLTTEHTTVAVTVKGVSEV
EHYTPAHVEEKLGITPAQIIDMKGLTGDTSDNYPGVTKVGEKTALKLLKQYGTMEGIYENIDAMKQSKMKEHLIEDREQA
AQAKVLATIKRDAPIDVQLADLSYDGPDLDQLAAFYQKMDFQSFLKKLHLQSDEPATPIDYTVLTKANLTELQQFTDHVD
FYLEMPTPNYHTSAFAGFAIGADDHWYISRDVELLMQPTVADLLSSTTITKDVFDMKRTTVGLNRLGIQLGKVDFDLLLV
SYLLDTNDNSNDLGSLAEQHGYTDLPSDETVYGKGVKRAIPEDDDVFFSHLARKLAAIASLKPQLLKELAANEQDQLYDD
IEKPMANVLAQMEIAGITVDSSRLKAMGSQFTERLSEIEQTIYQEAGEEFNINSPKQLGHILFEEMKLPVIKKTKTGYST
AVDVLEKLAPEAPIVANILQYRQISKIQSTYVVGLLDAIHSADQKVHTRYLQTLTQTGRLSSVDPNLQNIPVRLEEGRKI
RQAFVPSHEGWQIFSSDYSQIELRVLAHITDDENMQAAFKAGDDIHAATAMRIFHLNSAEEVTPNIRRQAKAVNFGIVYG
ISDYGLSQNIGITRKQARSFIDAYFKEYPGVKKYMDEIVQTAKKQGYVETISHRRRYLPDINSKSFNQRSFAERTAMNTP
IQGSAADIIKIAMIKMQDKLQAAGLKATMLLQVHDELIFEAPKEEIPVLEKLVPSVMDSAVQLAVPLKVESHYGDTWYDA
K

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=518, Percent_Identity=30.3088803088803, Blast_Score=214, Evalue=3e-55,
Organism=Homo sapiens, GI139394648, Length=528, Percent_Identity=28.7878787878788, Blast_Score=213, Evalue=8e-55,
Organism=Escherichia coli, GI1790294, Length=929, Percent_Identity=36.7061356297094, Blast_Score=505, Evalue=1e-144,
Organism=Escherichia coli, GI226510970, Length=220, Percent_Identity=29.5454545454545, Blast_Score=115, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI86563272, Length=541, Percent_Identity=25.8780036968577, Blast_Score=180, Evalue=3e-45,
Organism=Drosophila melanogaster, GI17933644, Length=422, Percent_Identity=33.175355450237, Blast_Score=239, Evalue=5e-63,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 98803; Mature: 98672

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKLLLIDGNSVAFRAFFALHSQLERFVNPDGLHTNAIYGFNTMLDHMIKAVEPTDMLV
CCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHCCCCCEEE
AFDAGKTTFRTAMYDNYKGGRAKTPSEFSEQMPYIKQLLDAYGIKHYELKDYEADDIIGT
EEECCCCHHHHHHHCCCCCCCCCCCHHHHHCCHHHHHHHHHHCCCEEEECCCCHHHHHHH
LAAQADQTGYTTTVVTGDRDLTQLTTEHTTVAVTVKGVSEVEHYTPAHVEEKLGITPAQI
HHHHCCCCCCEEEEEECCCHHHHHHHCCEEEEEEECCHHHHHHCCCCHHHHHCCCCHHHH
IDMKGLTGDTSDNYPGVTKVGEKTALKLLKQYGTMEGIYENIDAMKQSKMKEHLIEDREQ
EECCCCCCCCCCCCCCCHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AAQAKVLATIKRDAPIDVQLADLSYDGPDLDQLAAFYQKMDFQSFLKKLHLQSDEPATPI
HHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCC
DYTVLTKANLTELQQFTDHVDFYLEMPTPNYHTSAFAGFAIGADDHWYISRDVELLMQPT
CEEEEECCCHHHHHHHHCCCEEEEECCCCCCCCHHHCCEEECCCCCEEEECCHHHHHCCH
VADLLSSTTITKDVFDMKRTTVGLNRLGIQLGKVDFDLLLVSYLLDTNDNSNDLGSLAEQ
HHHHHHCCHHHHHHHHHHHHHHCHHHHHHEECCCHHHHHHHHHHHCCCCCCHHHHHHHHH
HGYTDLPSDETVYGKGVKRAIPEDDDVFFSHLARKLAAIASLKPQLLKELAANEQDQLYD
CCCCCCCCCCCEECCCHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHH
DIEKPMANVLAQMEIAGITVDSSRLKAMGSQFTERLSEIEQTIYQEAGEEFNINSPKQLG
HHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
HILFEEMKLPVIKKTKTGYSTAVDVLEKLAPEAPIVANILQYRQISKIQSTYVVGLLDAI
HHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
HSADQKVHTRYLQTLTQTGRLSSVDPNLQNIPVRLEEGRKIRQAFVPSHEGWQIFSSDYS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHHCCCCCCCEEECCCCH
QIELRVLAHITDDENMQAAFKAGDDIHAATAMRIFHLNSAEEVTPNIRRQAKAVNFGIVY
HEEEEEEEEECCCCCHHHHHHCCCCHHHHHHHHEEECCCHHHHCHHHHHHHHHCCEEEEE
GISDYGLSQNIGITRKQARSFIDAYFKEYPGVKKYMDEIVQTAKKQGYVETISHRRRYLP
EECCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCC
DINSKSFNQRSFAERTAMNTPIQGSAADIIKIAMIKMQDKLQAAGLKATMLLQVHDELIF
CCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
EAPKEEIPVLEKLVPSVMDSAVQLAVPLKVESHYGDTWYDAK
CCCHHHCCHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
AKKLLLIDGNSVAFRAFFALHSQLERFVNPDGLHTNAIYGFNTMLDHMIKAVEPTDMLV
CCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHCCCCCEEE
AFDAGKTTFRTAMYDNYKGGRAKTPSEFSEQMPYIKQLLDAYGIKHYELKDYEADDIIGT
EEECCCCHHHHHHHCCCCCCCCCCCHHHHHCCHHHHHHHHHHCCCEEEECCCCHHHHHHH
LAAQADQTGYTTTVVTGDRDLTQLTTEHTTVAVTVKGVSEVEHYTPAHVEEKLGITPAQI
HHHHCCCCCCEEEEEECCCHHHHHHHCCEEEEEEECCHHHHHHCCCCHHHHHCCCCHHHH
IDMKGLTGDTSDNYPGVTKVGEKTALKLLKQYGTMEGIYENIDAMKQSKMKEHLIEDREQ
EECCCCCCCCCCCCCCCHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AAQAKVLATIKRDAPIDVQLADLSYDGPDLDQLAAFYQKMDFQSFLKKLHLQSDEPATPI
HHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCC
DYTVLTKANLTELQQFTDHVDFYLEMPTPNYHTSAFAGFAIGADDHWYISRDVELLMQPT
CEEEEECCCHHHHHHHHCCCEEEEECCCCCCCCHHHCCEEECCCCCEEEECCHHHHHCCH
VADLLSSTTITKDVFDMKRTTVGLNRLGIQLGKVDFDLLLVSYLLDTNDNSNDLGSLAEQ
HHHHHHCCHHHHHHHHHHHHHHCHHHHHHEECCCHHHHHHHHHHHCCCCCCHHHHHHHHH
HGYTDLPSDETVYGKGVKRAIPEDDDVFFSHLARKLAAIASLKPQLLKELAANEQDQLYD
CCCCCCCCCCCEECCCHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHH
DIEKPMANVLAQMEIAGITVDSSRLKAMGSQFTERLSEIEQTIYQEAGEEFNINSPKQLG
HHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
HILFEEMKLPVIKKTKTGYSTAVDVLEKLAPEAPIVANILQYRQISKIQSTYVVGLLDAI
HHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
HSADQKVHTRYLQTLTQTGRLSSVDPNLQNIPVRLEEGRKIRQAFVPSHEGWQIFSSDYS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHHCCCCCCCEEECCCCH
QIELRVLAHITDDENMQAAFKAGDDIHAATAMRIFHLNSAEEVTPNIRRQAKAVNFGIVY
HEEEEEEEEECCCCCHHHHHHCCCCHHHHHHHHEEECCCHHHHCHHHHHHHHHCCEEEEE
GISDYGLSQNIGITRKQARSFIDAYFKEYPGVKKYMDEIVQTAKKQGYVETISHRRRYLP
EECCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCC
DINSKSFNQRSFAERTAMNTPIQGSAADIIKIAMIKMQDKLQAAGLKATMLLQVHDELIF
CCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
EAPKEEIPVLEKLVPSVMDSAVQLAVPLKVESHYGDTWYDAK
CCCHHHCCHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]