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The map label for this gene is oppB [H]

Identifier: 28378028

GI number: 28378028

Start: 1148278

End: 1149207

Strand: Direct

Name: oppB [H]

Synonym: lp_1262

Alternate gene names: 28378028

Gene position: 1148278-1149207 (Clockwise)

Preceding gene: 28378027

Following gene: 28378029

Centisome position: 34.71

GC content: 47.2

Gene sequence:

>930_bases
ATGAGAAAGTATCTTTTAAAGCGGATATTTTACTTGTTCCTGACATTATTCATTGTGGCGACCGCCACGTTCTTCTTAAT
GAAGTTAATGCCCGGAACACCGCTGCAAAATGAAGCTAAATTGACCCCTAGCGAAAAGGCGTCGATCTTGGCCCAATACG
GGTTGGACAAACCTGTTTGGTTGCAATACTTCATTTACATTGGCGGGATCTTCAAGGGGAGCTTAGGGCTATCCTTCCAG
TTCTCTGATCAAGCCGTCAGTTACCTGATTGGTAGCCGGATGGGACCATCAATGCAGTTAGGTGGCCAAGCGATGGTCGT
CGGAATCGTCTTTGGGATTATCCTAGGGGCCGTTGGTGCCATCAAGAAGGATACTTGGGCCGACCGTTTAGCAACCATCT
TATCGATCTTAGGGATCGCAGTTCCAAGTTTCGTGTTAGCCATCCTGTTACAATACTACCTCGGCTTAAAGGCTGGTTGG
TTCCCAGTTGCTGGCTGGGAAGGCTTCGCTTACACGATCTTACCAACGTTAGCTTTGGCCGCCAGTCCGCTGGCCGAAAC
GGCTCGGTTTATCCGAACCGACATGGTGGACGTGCTGAGTTCGGATTATATTGAGCTAGCTAAAGCCAAAGGGTTGTCGA
AGTTTGGTATCGTGTATCACCATGCGTTGCGGAACAGTTTGATTCCAATGGTTACGCTGATTGGACCATTGGCCGCCGCC
TTGATGACGGGTTCAATGGTTGTTGAAAATATCTTCTCAGTACCTGGGATTGGGGAACAATTCGTTAAATCAATTCTGGT
TAACGACTACCCAACCATCATGGGTGTGACGATTTTCTATTCAGCACTCCTTTGCTTACTATTATTGTTGACCGATATTG
TCTACGGAATTATTGATCCGCGGATTCGTTTAAATGGAAATGGGGACTAG

Upstream 100 bases:

>100_bases
GGGGTAAGGATTGACACATTGTTAATCCTTACCATTTTGTTTTATAATGAGGGATGTCATTATTTTTTAAGTTGATTTTA
ATAGAATGCGAGGAATTCTC

Downstream 100 bases:

>100_bases
TTAATGGCAGAAAATCCAAGTAGTATCGATATTAAGCCCGGCGACTTCGAGCCGTTAGATAAACAAGCGGGTCCTGATAA
TGAACGGATCGCAGCACCTT

Product: oligopeptide ABC transporter, permease protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: Stage 0 sporulation protein KB [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MRKYLLKRIFYLFLTLFIVATATFFLMKLMPGTPLQNEAKLTPSEKASILAQYGLDKPVWLQYFIYIGGIFKGSLGLSFQ
FSDQAVSYLIGSRMGPSMQLGGQAMVVGIVFGIILGAVGAIKKDTWADRLATILSILGIAVPSFVLAILLQYYLGLKAGW
FPVAGWEGFAYTILPTLALAASPLAETARFIRTDMVDVLSSDYIELAKAKGLSKFGIVYHHALRNSLIPMVTLIGPLAAA
LMTGSMVVENIFSVPGIGEQFVKSILVNDYPTIMGVTIFYSALLCLLLLLTDIVYGIIDPRIRLNGNGD

Sequences:

>Translated_309_residues
MRKYLLKRIFYLFLTLFIVATATFFLMKLMPGTPLQNEAKLTPSEKASILAQYGLDKPVWLQYFIYIGGIFKGSLGLSFQ
FSDQAVSYLIGSRMGPSMQLGGQAMVVGIVFGIILGAVGAIKKDTWADRLATILSILGIAVPSFVLAILLQYYLGLKAGW
FPVAGWEGFAYTILPTLALAASPLAETARFIRTDMVDVLSSDYIELAKAKGLSKFGIVYHHALRNSLIPMVTLIGPLAAA
LMTGSMVVENIFSVPGIGEQFVKSILVNDYPTIMGVTIFYSALLCLLLLLTDIVYGIIDPRIRLNGNGD
>Mature_309_residues
MRKYLLKRIFYLFLTLFIVATATFFLMKLMPGTPLQNEAKLTPSEKASILAQYGLDKPVWLQYFIYIGGIFKGSLGLSFQ
FSDQAVSYLIGSRMGPSMQLGGQAMVVGIVFGIILGAVGAIKKDTWADRLATILSILGIAVPSFVLAILLQYYLGLKAGW
FPVAGWEGFAYTILPTLALAASPLAETARFIRTDMVDVLSSDYIELAKAKGLSKFGIVYHHALRNSLIPMVTLIGPLAAA
LMTGSMVVENIFSVPGIGEQFVKSILVNDYPTIMGVTIFYSALLCLLLLLTDIVYGIIDPRIRLNGNGD

Specific function: Part of the binding-protein-dependent transport system for oligopeptides; probably responsible for the translocation of the substrate across the membrane. Also required for sporulation and competence [H]

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787497, Length=305, Percent_Identity=38.3606557377049, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1787053, Length=307, Percent_Identity=37.4592833876222, Blast_Score=211, Evalue=5e-56,
Organism=Escherichia coli, GI1789965, Length=337, Percent_Identity=31.4540059347181, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1789888, Length=309, Percent_Identity=31.3915857605178, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1787761, Length=338, Percent_Identity=31.9526627218935, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1787550, Length=273, Percent_Identity=26.007326007326, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1788504, Length=365, Percent_Identity=24.1095890410959, Blast_Score=105, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33684; Mature: 33684

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKYLLKRIFYLFLTLFIVATATFFLMKLMPGTPLQNEAKLTPSEKASILAQYGLDKPVW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHH
LQYFIYIGGIFKGSLGLSFQFSDQAVSYLIGSRMGPSMQLGGQAMVVGIVFGIILGAVGA
HHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IKKDTWADRLATILSILGIAVPSFVLAILLQYYLGLKAGWFPVAGWEGFAYTILPTLALA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
ASPLAETARFIRTDMVDVLSSDYIELAKAKGLSKFGIVYHHALRNSLIPMVTLIGPLAAA
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMTGSMVVENIFSVPGIGEQFVKSILVNDYPTIMGVTIFYSALLCLLLLLTDIVYGIIDP
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RIRLNGNGD
CEEECCCCC
>Mature Secondary Structure
MRKYLLKRIFYLFLTLFIVATATFFLMKLMPGTPLQNEAKLTPSEKASILAQYGLDKPVW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHH
LQYFIYIGGIFKGSLGLSFQFSDQAVSYLIGSRMGPSMQLGGQAMVVGIVFGIILGAVGA
HHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IKKDTWADRLATILSILGIAVPSFVLAILLQYYLGLKAGWFPVAGWEGFAYTILPTLALA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
ASPLAETARFIRTDMVDVLSSDYIELAKAKGLSKFGIVYHHALRNSLIPMVTLIGPLAAA
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMTGSMVVENIFSVPGIGEQFVKSILVNDYPTIMGVTIFYSALLCLLLLLTDIVYGIIDP
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RIRLNGNGD
CEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1901616; 1899858; 9384377 [H]