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The map label for this gene is oppA [H]

Identifier: 28378027

GI number: 28378027

Start: 1146477

End: 1148120

Strand: Direct

Name: oppA [H]

Synonym: lp_1261

Alternate gene names: 28378027

Gene position: 1146477-1148120 (Clockwise)

Preceding gene: 28378026

Following gene: 28378028

Centisome position: 34.65

GC content: 43.43

Gene sequence:

>1644_bases
ATGAATTTCAAAACAGCTGCAAAAGTAACCGTCGTTGCCGGCGCATCCGTACTATTCTTAGCTGCTTGTGGCTCAAGCAA
GAGTTCTTCAAGTTCTAAGCAAACGGCCAATTGGACCGAATCAGCCGAATTACCAACGATGGACATTTCTAAGTCCACCG
ATGTGGTTAGTTCTAACGCGTTGAATAACACCAATGAAGGGTTATACCGTCTCGGTAAGAACGGGAAGGTCACACCCGGA
ATTGCGAAATCCACTAAAGTTACTAATGGTGGGAAGACATATACGTTCACTTTACGGAAGAACGCAAAATGGAGTAACGG
GGATAAAGTTACTGCCAAAGACTTTGTTTATGGTTGGCAACGGACCGTTAATCCAAAGACTGCGTCACAATACGCATACT
TATATTCAGGGATTAAGAACGCTGACGCTATCGTCAATGGCAAGAAGCCCGTTTCTAGTTTAGGGATCAAGGCTGATGGC
GATTACAAGTTGACGGTCACTTTGGATAAGCCAATTGCTTACTTTGACAAGCTGATGGGCTTTGTCAACTTCTTCCCACA
AAATGAAAAAGTGGTTAAGAAGTACGGTTCTAAGTATGGGACGAGCAGTAAGGCCATGGTTTATAACGGCCCTTACAAGA
TGACTGGTTGGACTGGGACAAACTTGTCATGGACCTTGAAGAAGAATAACGATTATTGGGATAAGAAAGCTGTTAAGATG
GACAGCATCAAGTACCAAGTCGTTAAGGATCCTTCAACTGGCTTGAACTTATTTAATTCCAATAAGCTCGACTTTACGAC
ACTTTCCAACGAACAAGTTAAGAATTATAAGAATGATAAGAACTACTTAGTTCGGAAGACGGCTTCAACTTTCTACGTTG
AATTTAATCGTAAGGATAGCGATAAGACTTTAGCCAAAGCTTTCCAGAACAAGAAGATTCGTCAGGCCTTGTCATTAGCA
ATTAACCGGAAGTCCTACGTTAATAATACGTTAGGTAACGGTTCGACCGTTTCTAAAGGTTACGTTTCTGAAGACTTAGC
TAAAAACCCGACAACTGGTAAGGACTTTACGACTGACGCTGCCGTTCCTTCTGCAGTTGACTACAACTTAACGAAAGCTA
AGAAGTTGTGGAAAGAAGGTTTGAAGGAAATCGGTGAAACTAAGTTGAACATCAAGTTACTTTCTGATGATACGGATGCT
GGTAAGAAAGCCACTGAGTTCATTCAAGGTGGTTGGGAGAAATTACCTGGTCTGACCGTTACTAACCAGAACGTCCCATT
CAAGACCCGTCTTCAACGGTCACAAAACGGGAACTTCGATACGGTTATCTCTGCCTGGAATGCTGACTTTACTGACCCAA
TCTCCTTCTTGTCACTCTTCACGTCCGACAACTCATACAACAATGGGAAGTATGACAGTGCCGCATACGACGATGCTGTG
AAGAAGGCGGAAGGTGCCGATGCCAATAACAAGACTGCACGTTGGGCTGACATGGTCGCTGCTGAAAAGCAATTGATGAA
TGATCAAGGGGTCATCCCAATCTATCAACAAGCCTCAGCAACGTTGACTCGGAGCAATCTGAAGGGCATCATCTACAACA
CTGCGGGCTCAAACTACAACTTCAAGTACATGTCCGTAAAATAA

Upstream 100 bases:

>100_bases
GAGTTTAGGATTGATTTTAATTTATTTATAATATACAATTACTTTAACTTTTCGAGGGAAAAGCACACACAAACTATTTT
AAAGTTAGGGGTTGGGTTAT

Downstream 100 bases:

>100_bases
TGATGTAAATCCACAGAAAAAAGTGTGTGTGCTTTAACTGAATGAATGTGAAGCAAAGGGGTAAGGATTGACACATTGTT
AATCCTTACCATTTTGTTTT

Product: oligopeptide ABC transporter, substrate binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 547; Mature: 547

Protein sequence:

>547_residues
MNFKTAAKVTVVAGASVLFLAACGSSKSSSSSKQTANWTESAELPTMDISKSTDVVSSNALNNTNEGLYRLGKNGKVTPG
IAKSTKVTNGGKTYTFTLRKNAKWSNGDKVTAKDFVYGWQRTVNPKTASQYAYLYSGIKNADAIVNGKKPVSSLGIKADG
DYKLTVTLDKPIAYFDKLMGFVNFFPQNEKVVKKYGSKYGTSSKAMVYNGPYKMTGWTGTNLSWTLKKNNDYWDKKAVKM
DSIKYQVVKDPSTGLNLFNSNKLDFTTLSNEQVKNYKNDKNYLVRKTASTFYVEFNRKDSDKTLAKAFQNKKIRQALSLA
INRKSYVNNTLGNGSTVSKGYVSEDLAKNPTTGKDFTTDAAVPSAVDYNLTKAKKLWKEGLKEIGETKLNIKLLSDDTDA
GKKATEFIQGGWEKLPGLTVTNQNVPFKTRLQRSQNGNFDTVISAWNADFTDPISFLSLFTSDNSYNNGKYDSAAYDDAV
KKAEGADANNKTARWADMVAAEKQLMNDQGVIPIYQQASATLTRSNLKGIIYNTAGSNYNFKYMSVK

Sequences:

>Translated_547_residues
MNFKTAAKVTVVAGASVLFLAACGSSKSSSSSKQTANWTESAELPTMDISKSTDVVSSNALNNTNEGLYRLGKNGKVTPG
IAKSTKVTNGGKTYTFTLRKNAKWSNGDKVTAKDFVYGWQRTVNPKTASQYAYLYSGIKNADAIVNGKKPVSSLGIKADG
DYKLTVTLDKPIAYFDKLMGFVNFFPQNEKVVKKYGSKYGTSSKAMVYNGPYKMTGWTGTNLSWTLKKNNDYWDKKAVKM
DSIKYQVVKDPSTGLNLFNSNKLDFTTLSNEQVKNYKNDKNYLVRKTASTFYVEFNRKDSDKTLAKAFQNKKIRQALSLA
INRKSYVNNTLGNGSTVSKGYVSEDLAKNPTTGKDFTTDAAVPSAVDYNLTKAKKLWKEGLKEIGETKLNIKLLSDDTDA
GKKATEFIQGGWEKLPGLTVTNQNVPFKTRLQRSQNGNFDTVISAWNADFTDPISFLSLFTSDNSYNNGKYDSAAYDDAV
KKAEGADANNKTARWADMVAAEKQLMNDQGVIPIYQQASATLTRSNLKGIIYNTAGSNYNFKYMSVK
>Mature_547_residues
MNFKTAAKVTVVAGASVLFLAACGSSKSSSSSKQTANWTESAELPTMDISKSTDVVSSNALNNTNEGLYRLGKNGKVTPG
IAKSTKVTNGGKTYTFTLRKNAKWSNGDKVTAKDFVYGWQRTVNPKTASQYAYLYSGIKNADAIVNGKKPVSSLGIKADG
DYKLTVTLDKPIAYFDKLMGFVNFFPQNEKVVKKYGSKYGTSSKAMVYNGPYKMTGWTGTNLSWTLKKNNDYWDKKAVKM
DSIKYQVVKDPSTGLNLFNSNKLDFTTLSNEQVKNYKNDKNYLVRKTASTFYVEFNRKDSDKTLAKAFQNKKIRQALSLA
INRKSYVNNTLGNGSTVSKGYVSEDLAKNPTTGKDFTTDAAVPSAVDYNLTKAKKLWKEGLKEIGETKLNIKLLSDDTDA
GKKATEFIQGGWEKLPGLTVTNQNVPFKTRLQRSQNGNFDTVISAWNADFTDPISFLSLFTSDNSYNNGKYDSAAYDDAV
KKAEGADANNKTARWADMVAAEKQLMNDQGVIPIYQQASATLTRSNLKGIIYNTAGSNYNFKYMSVK

Specific function: Part of the binding-protein-dependent transport system for dipeptides; probably responsible for the binding of dipeptides with high affinity. Is expressed to facilitate adaptation to nutrient deficiency conditions, which also induce sporulation [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=534, Percent_Identity=31.8352059925094, Blast_Score=226, Evalue=4e-60,
Organism=Escherichia coli, GI1789397, Length=530, Percent_Identity=28.3018867924528, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI87081878, Length=505, Percent_Identity=29.3069306930693, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI1787052, Length=424, Percent_Identity=23.5849056603774, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789966, Length=505, Percent_Identity=22.7722772277228, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787762, Length=463, Percent_Identity=22.4622030237581, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60334; Mature: 60334

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFKTAAKVTVVAGASVLFLAACGSSKSSSSSKQTANWTESAELPTMDISKSTDVVSSNA
CCCCCCEEEEEEECCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHCCC
LNNTNEGLYRLGKNGKVTPGIAKSTKVTNGGKTYTFTLRKNAKWSNGDKVTAKDFVYGWQ
CCCCCCHHHEECCCCCCCCCCCCCCEECCCCCEEEEEEECCCCCCCCCEEEHHHHHHHHH
RTVNPKTASQYAYLYSGIKNADAIVNGKKPVSSLGIKADGDYKLTVTLDKPIAYFDKLMG
HCCCCCHHHHHHHHHHCCCCCCCEECCCCCHHHCCEECCCCEEEEEEECCCHHHHHHHHH
FVNFFPQNEKVVKKYGSKYGTSSKAMVYNGPYKMTGWTGTNLSWTLKKNNDYWDKKAVKM
HHHHCCCCHHHHHHHHHHCCCCCCEEEECCCEEECCCCCCCEEEEEECCCCCCHHHHEEE
DSIKYQVVKDPSTGLNLFNSNKLDFTTLSNEQVKNYKNDKNYLVRKTASTFYVEFNRKDS
CCEEEEEEECCCCCCEEECCCCEEEEECCCHHHHHCCCCCCEEEEEECEEEEEEECCCCC
DKTLAKAFQNKKIRQALSLAINRKSYVNNTLGNGSTVSKGYVSEDLAKNPTTGKDFTTDA
HHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCC
AVPSAVDYNLTKAKKLWKEGLKEIGETKLNIKLLSDDTDAGKKATEFIQGGWEKLPGLTV
CCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHCHHHHCCCEEE
TNQNVPFKTRLQRSQNGNFDTVISAWNADFTDPISFLSLFTSDNSYNNGKYDSAAYDDAV
ECCCCCHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHH
KKAEGADANNKTARWADMVAAEKQLMNDQGVIPIYQQASATLTRSNLKGIIYNTAGSNYN
HHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCEEECCCCCEEEEECCCCCEE
FKYMSVK
EEEEEEC
>Mature Secondary Structure
MNFKTAAKVTVVAGASVLFLAACGSSKSSSSSKQTANWTESAELPTMDISKSTDVVSSNA
CCCCCCEEEEEEECCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHCCC
LNNTNEGLYRLGKNGKVTPGIAKSTKVTNGGKTYTFTLRKNAKWSNGDKVTAKDFVYGWQ
CCCCCCHHHEECCCCCCCCCCCCCCEECCCCCEEEEEEECCCCCCCCCEEEHHHHHHHHH
RTVNPKTASQYAYLYSGIKNADAIVNGKKPVSSLGIKADGDYKLTVTLDKPIAYFDKLMG
HCCCCCHHHHHHHHHHCCCCCCCEECCCCCHHHCCEECCCCEEEEEEECCCHHHHHHHHH
FVNFFPQNEKVVKKYGSKYGTSSKAMVYNGPYKMTGWTGTNLSWTLKKNNDYWDKKAVKM
HHHHCCCCHHHHHHHHHHCCCCCCEEEECCCEEECCCCCCCEEEEEECCCCCCHHHHEEE
DSIKYQVVKDPSTGLNLFNSNKLDFTTLSNEQVKNYKNDKNYLVRKTASTFYVEFNRKDS
CCEEEEEEECCCCCCEEECCCCEEEEECCCHHHHHCCCCCCEEEEEECEEEEEEECCCCC
DKTLAKAFQNKKIRQALSLAINRKSYVNNTLGNGSTVSKGYVSEDLAKNPTTGKDFTTDA
HHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCC
AVPSAVDYNLTKAKKLWKEGLKEIGETKLNIKLLSDDTDAGKKATEFIQGGWEKLPGLTV
CCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHCHHHHCCCEEE
TNQNVPFKTRLQRSQNGNFDTVISAWNADFTDPISFLSLFTSDNSYNNGKYDSAAYDDAV
ECCCCCHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHH
KKAEGADANNKTARWADMVAAEKQLMNDQGVIPIYQQASATLTRSNLKGIIYNTAGSNYN
HHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCEEECCCCCEEEEECCCCCEE
FKYMSVK
EEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1766370; 9384377 [H]