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The map label for this gene is mae [C]

Identifier: 28377893

GI number: 28377893

Start: 1003206

End: 1004366

Strand: Direct

Name: mae [C]

Synonym: lp_1105

Alternate gene names: 28377893

Gene position: 1003206-1004366 (Clockwise)

Preceding gene: 28377891

Following gene: 28377894

Centisome position: 30.32

GC content: 51.16

Gene sequence:

>1161_bases
ATGGTAGAACAAGATGAGATTTTAAAGTTGCACGCCGCCCATACGGGGGTTTTGGACATTCATTCAGACCTTGCAGTGAC
CGATAAGGCTGATTTAGGTAAAGCATATACACCTGGTGTTGCGGTGATTTCCAAGCTGATTGCGGCGCATCCGGAATTAA
AGGCAAAATACACGATGAGTGGTAAATTAGTAGCGTTGATCACGGATGGGTCAGCAGTACTGGGCCTCGGCAATATTGGT
CCCGCGGGTGGTTTGCCGGTCGTAGAAGGTAAGGCACTCCTATACAAGAACCTCGCTGGGATTAATGCGATTCCGCTGGC
GTTAAATCAGGTCAGCGTGCCAGCAATGGTGGCGACAATCAAAAACATGTCGCTCTCATTTGCGGGCATTCATCTGGAAG
ATATTACGGCGCCTAAGTGTTTTGAACTAGAAGCGGCCTTGAAAGATGAGCTTGATATTCCGGTTTATCATGACGATCAG
GAAGGCACCGCAATTGTCGTGTTGGCGGGACTGATCAATGCCGCTCACGTAGTCGGTAAGTCCTTACGGCAGATGCGCAT
CGTTGTCAACGGCGTCGGCGCATCCGGGTTAGCTACAGCTCGGTTATTAGCAGCGGTCGGTGTCAAACAACTGACCTTGG
TCGATGTTTACGGGGTCGTTACAGCGGATGATCAGCGGTACAACCAGTATCAACGGAATCTTGCCAAACAGTTGGGAACA
CCTGCACAACTAAAGGGGCAACCACTGGCGAAGGTCATGGCCGACCAGGACGTCTTCATTGGCTTGTCAGACGCCAACGT
CTTATCAGCAGGGGCCGTCCACTTGATGAATCATGATCCAATCGTCTTTGCACTCGCTAATCCAGTGCCAGAAATCAATC
CAGCAGTAGCTGAGAAGGCTGGGGCCACAGTCATTGCAACAGGTTCTAGCCAGTATCCAAACCAGGTCAATAATATTCTG
GCTTTCCCGGGGCTCTTCAAGGGCTTACTCACAAATGGGGTCAAGCAAGTTGATAATGCGTTGCAGATCCAAGTGGCTCA
GGCTTTGGCTAAGACCGTGTCGAACCCAACTGCGACGACGATTGTGCCGAGCGTCTTTGATTCACAAGTGGTACCGAACG
TGACGGCGGCCGTTGCAGCGTACGCGCAAGCACAGCATTAA

Upstream 100 bases:

>100_bases
TTATAAAAATCCAATCTAAATTTGATATTTTTAAAAAATGGTCGTACACTGGGGATAACAAAGTGAACGGTATTATTTTG
AGAAGAGAAGGCAGATATTG

Downstream 100 bases:

>100_bases
TCATAGGAGGTTGTCTGATGGCAGCGCAAGTTGTTGAAATGCAATTAAATAATCCGCACGAGCATGCCCAGTGGCAACAA
TTCTTAACGTCATTAGGGAT

Product: malic enzyme, NAD-dependent

Products: NA

Alternate protein names: NAD-ME [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MVEQDEILKLHAAHTGVLDIHSDLAVTDKADLGKAYTPGVAVISKLIAAHPELKAKYTMSGKLVALITDGSAVLGLGNIG
PAGGLPVVEGKALLYKNLAGINAIPLALNQVSVPAMVATIKNMSLSFAGIHLEDITAPKCFELEAALKDELDIPVYHDDQ
EGTAIVVLAGLINAAHVVGKSLRQMRIVVNGVGASGLATARLLAAVGVKQLTLVDVYGVVTADDQRYNQYQRNLAKQLGT
PAQLKGQPLAKVMADQDVFIGLSDANVLSAGAVHLMNHDPIVFALANPVPEINPAVAEKAGATVIATGSSQYPNQVNNIL
AFPGLFKGLLTNGVKQVDNALQIQVAQALAKTVSNPTATTIVPSVFDSQVVPNVTAAVAAYAQAQH

Sequences:

>Translated_386_residues
MVEQDEILKLHAAHTGVLDIHSDLAVTDKADLGKAYTPGVAVISKLIAAHPELKAKYTMSGKLVALITDGSAVLGLGNIG
PAGGLPVVEGKALLYKNLAGINAIPLALNQVSVPAMVATIKNMSLSFAGIHLEDITAPKCFELEAALKDELDIPVYHDDQ
EGTAIVVLAGLINAAHVVGKSLRQMRIVVNGVGASGLATARLLAAVGVKQLTLVDVYGVVTADDQRYNQYQRNLAKQLGT
PAQLKGQPLAKVMADQDVFIGLSDANVLSAGAVHLMNHDPIVFALANPVPEINPAVAEKAGATVIATGSSQYPNQVNNIL
AFPGLFKGLLTNGVKQVDNALQIQVAQALAKTVSNPTATTIVPSVFDSQVVPNVTAAVAAYAQAQH
>Mature_386_residues
MVEQDEILKLHAAHTGVLDIHSDLAVTDKADLGKAYTPGVAVISKLIAAHPELKAKYTMSGKLVALITDGSAVLGLGNIG
PAGGLPVVEGKALLYKNLAGINAIPLALNQVSVPAMVATIKNMSLSFAGIHLEDITAPKCFELEAALKDELDIPVYHDDQ
EGTAIVVLAGLINAAHVVGKSLRQMRIVVNGVGASGLATARLLAAVGVKQLTLVDVYGVVTADDQRYNQYQRNLAKQLGT
PAQLKGQPLAKVMADQDVFIGLSDANVLSAGAVHLMNHDPIVFALANPVPEINPAVAEKAGATVIATGSSQYPNQVNNIL
AFPGLFKGLLTNGVKQVDNALQIQVAQALAKTVSNPTATTIVPSVFDSQVVPNVTAAVAAYAQAQH

Specific function: In addition to the NAD-dependent oxidative decarboxylation of L-malate, the enzyme catalyzes the decarboxylation of oxaloacetate [H]

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=354, Percent_Identity=24.2937853107345, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1788806, Length=375, Percent_Identity=36, Blast_Score=236, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI17537199, Length=376, Percent_Identity=26.5957446808511, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24646388, Length=352, Percent_Identity=28.4090909090909, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24646386, Length=352, Percent_Identity=28.4090909090909, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 40102; Mature: 40102

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEQDEILKLHAAHTGVLDIHSDLAVTDKADLGKAYTPGVAVISKLIAAHPELKAKYTMS
CCCHHHHHEEECCCCCEEEECCCCEECCCHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEC
GKLVALITDGSAVLGLGNIGPAGGLPVVEGKALLYKNLAGINAIPLALNQVSVPAMVATI
CEEEEEEECCCEEEEECCCCCCCCCCEECCCHHHHHHHCCCCEEEHHHCCCCHHHHHHHH
KNMSLSFAGIHLEDITAPKCFELEAALKDELDIPVYHDDQEGTAIVVLAGLINAAHVVGK
HHCCEEEEEEEEECCCCCCCEEHHHHHHCCCCCCEEECCCCCCEEEEEHHHHHHHHHHHH
SLRQMRIVVNGVGASGLATARLLAAVGVKQLTLVDVYGVVTADDQRYNQYQRNLAKQLGT
HHHHEEEEEEECCCCHHHHHHHHHHHCHHHEEEEEEEEEEECCHHHHHHHHHHHHHHCCC
PAQLKGQPLAKVMADQDVFIGLSDANVLSAGAVHLMNHDPIVFALANPVPEINPAVAEKA
CCCCCCCCHHHHHCCCCEEEEECCCCCCCCCEEEEECCCCEEEEECCCCCCCCHHHHHHC
GATVIATGSSQYPNQVNNILAFPGLFKGLLTNGVKQVDNALQIQVAQALAKTVSNPTATT
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHE
IVPSVFDSQVVPNVTAAVAAYAQAQH
EEHHHHCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MVEQDEILKLHAAHTGVLDIHSDLAVTDKADLGKAYTPGVAVISKLIAAHPELKAKYTMS
CCCHHHHHEEECCCCCEEEECCCCEECCCHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEC
GKLVALITDGSAVLGLGNIGPAGGLPVVEGKALLYKNLAGINAIPLALNQVSVPAMVATI
CEEEEEEECCCEEEEECCCCCCCCCCEECCCHHHHHHHCCCCEEEHHHCCCCHHHHHHHH
KNMSLSFAGIHLEDITAPKCFELEAALKDELDIPVYHDDQEGTAIVVLAGLINAAHVVGK
HHCCEEEEEEEEECCCCCCCEEHHHHHHCCCCCCEEECCCCCCEEEEEHHHHHHHHHHHH
SLRQMRIVVNGVGASGLATARLLAAVGVKQLTLVDVYGVVTADDQRYNQYQRNLAKQLGT
HHHHEEEEEEECCCCHHHHHHHHHHHCHHHEEEEEEEEEEECCHHHHHHHHHHHHHHCCC
PAQLKGQPLAKVMADQDVFIGLSDANVLSAGAVHLMNHDPIVFALANPVPEINPAVAEKA
CCCCCCCCHHHHHCCCCEEEEECCCCCCCCCEEEEECCCCEEEEECCCCCCCCHHHHHHC
GATVIATGSSQYPNQVNNILAFPGLFKGLLTNGVKQVDNALQIQVAQALAKTVSNPTATT
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHE
IVPSVFDSQVVPNVTAAVAAYAQAQH
EEHHHHCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644282 [H]