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The map label for this gene is yflS [H]

Identifier: 28377891

GI number: 28377891

Start: 1000553

End: 1001971

Strand: Direct

Name: yflS [H]

Synonym: lp_1102

Alternate gene names: 28377891

Gene position: 1000553-1001971 (Clockwise)

Preceding gene: 28377890

Following gene: 28377893

Centisome position: 30.24

GC content: 46.86

Gene sequence:

>1419_bases
ATGACACTAAATAAGGTCAAGTATCGTGATTTTATTGCACCGTTATTGGTGGGCCTGATCATTTGGTTAAGTAGTCCTAT
CAAACCAGCCAGTGTTTCGATGAACGCATGGCATATTCTGGCAATTTTTGTGGCAACCATTATTGGTTGTATTACGCGGC
CGTTACCAATCGCCGGGGTCGCAATCATTGGGTTGACACTAACCGTGTTATTGGGCATCGTGCCTATGGATACAGCGGTT
GCCGGATTTGGGAGCAGTACGGTATGGATGATTGCGATGGCGTATTTTATGTCACGTGGTTTCATCAATACTGGTTTAGG
TCGACGTGTCGCGTTGGTGTTTGTGCGGCTGTTTGGGAAACGAACGTTGGGTTTGGCATACGCACTGATTGGCGTCGATT
TAGTAACAGCACCCGCAACGCCAAGCAATACGGCCCGTGCTGGTGGGATTGTTTATCCAATCATTCAGTCGTTAGCTGAA
ACGTTTGGTTCTGATCCCAAGGATGGCACACAAAGTAAAATCGGTTCATTCTTAGTGTTCGCAGAATTTCATGGTGATTT
AATCACCAGTACGATGTTCATGACGGCGATGGCGCCTAACTTAGTTGCTGTCACGTTAGCCAAGAGTTTACACATCACGT
TATCGTGGATGCAATGGTTCTTAGCCGGGATTGTCCCAGGACTTATTTGTTTATTGGTTGTGCCATATTTAATTTATAAA
ATGTATCCACCAGAAATTAAAGATACGCCCAATGCCAAGCAATGGGCAGATGATGAGCTGGCAAAGATGGGTAAGATGAG
TCTTCCAGAAAAAATCATGGGCGGCGTCTTCTTGCTAGCCTTAGTCTTATGGATGTTATCCAGCTTTATCGGTCTCGAAG
CTTCATTAGTTGCCTTTATGGCCGTCTCCTTGCTACTCGTTGCTGGAGTTCTATCGGTAGATGACGTGCTACACGAAACT
GGTGCTTGGAATACCTTGGTGTGGTTCTCAATTTTGATCTTCATGGCTAATGAGTTGAACAAACTCGGCTTTATTCCATG
GTTGTCTGGCGCGATTGGTGGTGCCTTGAAAGGCTTCAGTTGGGGCATTGTTTTAGCGGCACTCGTCTTGTTCTACTTCT
ACAGTCACTACTTGTTTGCTAGTGGGACTGCCCACGTTTCCGCAATGTACGGTGCCTTACTTGGGGTCGCGGTTTCCGCG
GGAGCGCCAGCAACCTTTGCTGCACTGATACTTGGCTTTACTGGTTCGATCTTCGCTTCGACGACGCATTATGCGAACGG
CCCGGCGAGTGTTCTCTTCGGTTCTGGGTATGTTACTCAGGGAAAATGGTGGCAGTTAAACTTTATTTTAGGTCTCTTCT
ACCTAGTCGTTTGGGGTGGCATTGGCGCCCTCTGGATGAAAGTGATTGGTATTTGGTAG

Upstream 100 bases:

>100_bases
GATGATGAGCGTGCCCGGATGCAACATTTCGCAGCCAAGATGCTGACCATGATGAATGTGGCATCATAAAAATGATGAGT
AAGTATGAGGAGGAATTGAG

Downstream 100 bases:

>100_bases
TCTGATTTTAAGCATAAAAACAGGTACCAAGCTGCCGGCAACGGTCAGCGTTGGTACCTGTTTTCGAAGAAGCGGATTAT
TTGGATAGTGGTGTGGCTTG

Product: cation transport protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MTLNKVKYRDFIAPLLVGLIIWLSSPIKPASVSMNAWHILAIFVATIIGCITRPLPIAGVAIIGLTLTVLLGIVPMDTAV
AGFGSSTVWMIAMAYFMSRGFINTGLGRRVALVFVRLFGKRTLGLAYALIGVDLVTAPATPSNTARAGGIVYPIIQSLAE
TFGSDPKDGTQSKIGSFLVFAEFHGDLITSTMFMTAMAPNLVAVTLAKSLHITLSWMQWFLAGIVPGLICLLVVPYLIYK
MYPPEIKDTPNAKQWADDELAKMGKMSLPEKIMGGVFLLALVLWMLSSFIGLEASLVAFMAVSLLLVAGVLSVDDVLHET
GAWNTLVWFSILIFMANELNKLGFIPWLSGAIGGALKGFSWGIVLAALVLFYFYSHYLFASGTAHVSAMYGALLGVAVSA
GAPATFAALILGFTGSIFASTTHYANGPASVLFGSGYVTQGKWWQLNFILGLFYLVVWGGIGALWMKVIGIW

Sequences:

>Translated_472_residues
MTLNKVKYRDFIAPLLVGLIIWLSSPIKPASVSMNAWHILAIFVATIIGCITRPLPIAGVAIIGLTLTVLLGIVPMDTAV
AGFGSSTVWMIAMAYFMSRGFINTGLGRRVALVFVRLFGKRTLGLAYALIGVDLVTAPATPSNTARAGGIVYPIIQSLAE
TFGSDPKDGTQSKIGSFLVFAEFHGDLITSTMFMTAMAPNLVAVTLAKSLHITLSWMQWFLAGIVPGLICLLVVPYLIYK
MYPPEIKDTPNAKQWADDELAKMGKMSLPEKIMGGVFLLALVLWMLSSFIGLEASLVAFMAVSLLLVAGVLSVDDVLHET
GAWNTLVWFSILIFMANELNKLGFIPWLSGAIGGALKGFSWGIVLAALVLFYFYSHYLFASGTAHVSAMYGALLGVAVSA
GAPATFAALILGFTGSIFASTTHYANGPASVLFGSGYVTQGKWWQLNFILGLFYLVVWGGIGALWMKVIGIW
>Mature_471_residues
TLNKVKYRDFIAPLLVGLIIWLSSPIKPASVSMNAWHILAIFVATIIGCITRPLPIAGVAIIGLTLTVLLGIVPMDTAVA
GFGSSTVWMIAMAYFMSRGFINTGLGRRVALVFVRLFGKRTLGLAYALIGVDLVTAPATPSNTARAGGIVYPIIQSLAET
FGSDPKDGTQSKIGSFLVFAEFHGDLITSTMFMTAMAPNLVAVTLAKSLHITLSWMQWFLAGIVPGLICLLVVPYLIYKM
YPPEIKDTPNAKQWADDELAKMGKMSLPEKIMGGVFLLALVLWMLSSFIGLEASLVAFMAVSLLLVAGVLSVDDVLHETG
AWNTLVWFSILIFMANELNKLGFIPWLSGAIGGALKGFSWGIVLAALVLFYFYSHYLFASGTAHVSAMYGALLGVAVSAG
APATFAALILGFTGSIFASTTHYANGPASVLFGSGYVTQGKWWQLNFILGLFYLVVWGGIGALWMKVIGIW

Specific function: Might be a malate transporter [H]

COG id: COG0471

COG function: function code P; Di- and tricarboxylate transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SLC13A transporter (TC 2.A.47) family [H]

Homologues:

Organism=Escherichia coli, GI1786829, Length=475, Percent_Identity=36.6315789473684, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI1789444, Length=485, Percent_Identity=36.4948453608247, Blast_Score=289, Evalue=3e-79,
Organism=Escherichia coli, GI1786986, Length=445, Percent_Identity=37.3033707865169, Blast_Score=275, Evalue=3e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001898 [H]

Pfam domain/function: PF00939 Na_sulph_symp [H]

EC number: NA

Molecular weight: Translated: 50647; Mature: 50516

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCEEECCCEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLNKVKYRDFIAPLLVGLIIWLSSPIKPASVSMNAWHILAIFVATIIGCITRPLPIAGV
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHH
AIIGLTLTVLLGIVPMDTAVAGFGSSTVWMIAMAYFMSRGFINTGLGRRVALVFVRLFGK
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHCC
RTLGLAYALIGVDLVTAPATPSNTARAGGIVYPIIQSLAETFGSDPKDGTQSKIGSFLVF
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHEEEE
AEFHGDLITSTMFMTAMAPNLVAVTLAKSLHITLSWMQWFLAGIVPGLICLLVVPYLIYK
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MYPPEIKDTPNAKQWADDELAKMGKMSLPEKIMGGVFLLALVLWMLSSFIGLEASLVAFM
CCCCCCCCCCCCHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
AVSLLLVAGVLSVDDVLHETGAWNTLVWFSILIFMANELNKLGFIPWLSGAIGGALKGFS
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCH
WGIVLAALVLFYFYSHYLFASGTAHVSAMYGALLGVAVSAGAPATFAALILGFTGSIFAS
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHC
TTHYANGPASVLFGSGYVTQGKWWQLNFILGLFYLVVWGGIGALWMKVIGIW
CCCCCCCCCCEEECCCEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377; 12949159 [H]