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The map label for this gene is rho [H]

Identifier: 28377399

GI number: 28377399

Start: 457163

End: 458455

Strand: Direct

Name: rho [H]

Synonym: lp_0511

Alternate gene names: 28377399

Gene position: 457163-458455 (Clockwise)

Preceding gene: 28377398

Following gene: 28377400

Centisome position: 13.82

GC content: 46.4

Gene sequence:

>1293_bases
ATGGAAAAAATTTTAACAATGCACGATCTCGAGCAGAAAACGTTAAAAGAAATTTATAACTATGCTCGTGAGTATAAAAT
TTCTTATTATTCGCAAATGAATAAAAAGGAACTCTCATTAGCCGTTCTCCGTGAGCAGGATAAAAAGCGGGGCTTTGTAC
AAATGGAAGGCGTCTTGGAAATTATTAGCCAAGAGGGCTATGGCTTTTTGCGGCCAATTAATTATGGGCCTTCGCAGGAA
GATATTTATATTTCTCAGTCGCAGATTCATCGCTTTGGTCTTCGTAACGGAGACTATGTGGCTGGTCAAGCCCGGCCACC
GCGACCTAACGAACGGTATTACGGTTTAATGCGGGTAGGGACGGTTAACGGTAAGGATCCTAATGAGGCCAAGCAACGGC
CCCATTTTCCAGCGTTAACACCACTTTATCCACAAAAACAAATTAAGTTATCGACAACGGCCGCCGTTTTAGCGACCCGT
TTGATTGATACTTTTGTCCCGATTGGCTTCGGTCAACGGGGCTTAATTGTCGCACCTCCTAAAGCCGGTAAGACGACGCT
GTTAAAGAATATTGCAAATGGCATCGTCGCCAATTATCCAGACGCTAAGCTGATTATGTTACTGATTGATGAACGGCCCG
AAGAAGTGACTGATTTAGAGCGGTCAATCAAGGGAGAAGTGGTGAGTTCTACTTTTGATCAACAACCACAAAATCACGTG
CGTGTTGCGGAATTGGTGCTTGAACGTGCGCGGCGCCTAGTTGAAGACAAACAGGATGTGATTATCTTATTGGATTCGAT
TACCCGGCTTACGCGGGCATATAATTTGGTCATCCCGTCTAGCGGGCGGACGTTGTCTGGTGGGGTCGATCCCACGGCGT
TTTACAAGCCCAAGCGATTCTTCGGTTCCGCGCGCAATATCGAGGAAGGTGGTAGCCTAACGATTTTAGGCACCGCGTTA
GTCGATACGGGTAGCCGGATGGACGACATGATTTATGAAGAATTCAAAGGAACTGGCAACTCAGAATTGCAGCTATCCCG
CGAGTTAGCTGAGCGGCGGGTCTTCCCGGCGCTAGACTTGAAGCAATCCAGTACGCGTAAAGAGGAATTGTTGGTTAGTC
GTAAGAATTTGGAGCCAGTCTGGCAGATTCGTCGATCAATGACTGGCAACGCGTTAGAGTATACCGAACAGCTTTTACAA
TTTTTAAAACACACGAAGACCAACACTGAGTTTGTTCGTGATTTAGCTAGCTTAAAGTTCACTGGTCAATCAACCCGGCG
GTCGCGTCGTTAA

Upstream 100 bases:

>100_bases
TCATCGATAAATTGCATACACTTGGTGCGGATGTGGAAATTGCAGCCGACGAAGAAGTTCCCGAAAACTAACGATGTACA
GCTAAGAAAGAACAGTGGTT

Downstream 100 bases:

>100_bases
TACTGGCAGGCAGGCTACTTTGTAGGTTGACGGGAAGCTGTGATTCGTGATAACATGTTTATCGTTGGTTAAAACTATGA
TAATCTCTGTTTCAGCAAAT

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MEKILTMHDLEQKTLKEIYNYAREYKISYYSQMNKKELSLAVLREQDKKRGFVQMEGVLEIISQEGYGFLRPINYGPSQE
DIYISQSQIHRFGLRNGDYVAGQARPPRPNERYYGLMRVGTVNGKDPNEAKQRPHFPALTPLYPQKQIKLSTTAAVLATR
LIDTFVPIGFGQRGLIVAPPKAGKTTLLKNIANGIVANYPDAKLIMLLIDERPEEVTDLERSIKGEVVSSTFDQQPQNHV
RVAELVLERARRLVEDKQDVIILLDSITRLTRAYNLVIPSSGRTLSGGVDPTAFYKPKRFFGSARNIEEGGSLTILGTAL
VDTGSRMDDMIYEEFKGTGNSELQLSRELAERRVFPALDLKQSSTRKEELLVSRKNLEPVWQIRRSMTGNALEYTEQLLQ
FLKHTKTNTEFVRDLASLKFTGQSTRRSRR

Sequences:

>Translated_430_residues
MEKILTMHDLEQKTLKEIYNYAREYKISYYSQMNKKELSLAVLREQDKKRGFVQMEGVLEIISQEGYGFLRPINYGPSQE
DIYISQSQIHRFGLRNGDYVAGQARPPRPNERYYGLMRVGTVNGKDPNEAKQRPHFPALTPLYPQKQIKLSTTAAVLATR
LIDTFVPIGFGQRGLIVAPPKAGKTTLLKNIANGIVANYPDAKLIMLLIDERPEEVTDLERSIKGEVVSSTFDQQPQNHV
RVAELVLERARRLVEDKQDVIILLDSITRLTRAYNLVIPSSGRTLSGGVDPTAFYKPKRFFGSARNIEEGGSLTILGTAL
VDTGSRMDDMIYEEFKGTGNSELQLSRELAERRVFPALDLKQSSTRKEELLVSRKNLEPVWQIRRSMTGNALEYTEQLLQ
FLKHTKTNTEFVRDLASLKFTGQSTRRSRR
>Mature_430_residues
MEKILTMHDLEQKTLKEIYNYAREYKISYYSQMNKKELSLAVLREQDKKRGFVQMEGVLEIISQEGYGFLRPINYGPSQE
DIYISQSQIHRFGLRNGDYVAGQARPPRPNERYYGLMRVGTVNGKDPNEAKQRPHFPALTPLYPQKQIKLSTTAAVLATR
LIDTFVPIGFGQRGLIVAPPKAGKTTLLKNIANGIVANYPDAKLIMLLIDERPEEVTDLERSIKGEVVSSTFDQQPQNHV
RVAELVLERARRLVEDKQDVIILLDSITRLTRAYNLVIPSSGRTLSGGVDPTAFYKPKRFFGSARNIEEGGSLTILGTAL
VDTGSRMDDMIYEEFKGTGNSELQLSRELAERRVFPALDLKQSSTRKEELLVSRKNLEPVWQIRRSMTGNALEYTEQLLQ
FLKHTKTNTEFVRDLASLKFTGQSTRRSRR

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=413, Percent_Identity=50.6053268765133, Blast_Score=422, Evalue=1e-119,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 48948; Mature: 48948

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKILTMHDLEQKTLKEIYNYAREYKISYYSQMNKKELSLAVLREQDKKRGFVQMEGVLE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHH
IISQEGYGFLRPINYGPSQEDIYISQSQIHRFGLRNGDYVAGQARPPRPNERYYGLMRVG
HHHCCCCCEEEECCCCCCCCCEEEEHHHHHHHCCCCCCEEECCCCCCCCCCCEEEEEEEE
TVNGKDPNEAKQRPHFPALTPLYPQKQIKLSTTAAVLATRLIDTFVPIGFGQRGLIVAPP
ECCCCCCCHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEECC
KAGKTTLLKNIANGIVANYPDAKLIMLLIDERPEEVTDLERSIKGEVVSSTFDQQPQNHV
CCCHHHHHHHHHHHHEECCCCCEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHCCCHHHH
RVAELVLERARRLVEDKQDVIILLDSITRLTRAYNLVIPSSGRTLSGGVDPTAFYKPKRF
HHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHEEEECCCCCEECCCCCCHHHCCCHHH
FGSARNIEEGGSLTILGTALVDTGSRMDDMIYEEFKGTGNSELQLSRELAERRVFPALDL
HCCCCCCCCCCCEEEEEEHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCC
KQSSTRKEELLVSRKNLEPVWQIRRSMTGNALEYTEQLLQFLKHTKTNTEFVRDLASLKF
CCCCCHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
TGQSTRRSRR
CCCHHHCCCC
>Mature Secondary Structure
MEKILTMHDLEQKTLKEIYNYAREYKISYYSQMNKKELSLAVLREQDKKRGFVQMEGVLE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHH
IISQEGYGFLRPINYGPSQEDIYISQSQIHRFGLRNGDYVAGQARPPRPNERYYGLMRVG
HHHCCCCCEEEECCCCCCCCCEEEEHHHHHHHCCCCCCEEECCCCCCCCCCCEEEEEEEE
TVNGKDPNEAKQRPHFPALTPLYPQKQIKLSTTAAVLATRLIDTFVPIGFGQRGLIVAPP
ECCCCCCCHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEECC
KAGKTTLLKNIANGIVANYPDAKLIMLLIDERPEEVTDLERSIKGEVVSSTFDQQPQNHV
CCCHHHHHHHHHHHHEECCCCCEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHCCCHHHH
RVAELVLERARRLVEDKQDVIILLDSITRLTRAYNLVIPSSGRTLSGGVDPTAFYKPKRF
HHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHEEEECCCCCEECCCCCCHHHCCCHHH
FGSARNIEEGGSLTILGTALVDTGSRMDDMIYEEFKGTGNSELQLSRELAERRVFPALDL
HCCCCCCCCCCCEEEEEEHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCC
KQSSTRKEELLVSRKNLEPVWQIRRSMTGNALEYTEQLLQFLKHTKTNTEFVRDLASLKF
CCCCCHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
TGQSTRRSRR
CCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423140; 9353933; 9384377 [H]