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The map label for this gene is rpoE

Identifier: 28377374

GI number: 28377374

Start: 428432

End: 429031

Strand: Direct

Name: rpoE

Synonym: lp_0480

Alternate gene names: 28377374

Gene position: 428432-429031 (Clockwise)

Preceding gene: 28377373

Following gene: 28377375

Centisome position: 12.95

GC content: 44.5

Gene sequence:

>600_bases
TTGGAACTAAAACAATTTGATGGACAAAAAAAGTCCGAATTATCTTTAATCGAGGTTGCTCACGCGATCTTATCACAACA
TGGTGATGTGATGGCTTTTGCCGACTTGACCAACGCTGTGCAATCATATTTAGGTAAAAGCGACGAAGAAATTCGGGAGC
GTTTATCACAATTTTATACCGATCTTAATATCGATGGTAGTTTCATTTCGCTCGGTGACAATATGTGGGGCCTGCGTGCA
TGGTACCCATTTGAATCCATTGACGAAGCCGTTATTCATACGGACGATGACGAAGATGAGGATCGGCCAAAGCGTAAGAA
GGTTAACGCCTTCTTGGCCGATGCCGGGGATGATGACGACGTGATCGATTACGATGACGATGATCCCGAAGATGACGATA
ACTACGATGACGATGATGATCAGGACGACGATACTGATGATTCAGCAAGCAGCAAGTACGATGAACTTGCGGGTGTTGAT
GACACAGACGACGATGTTGCTGATGAAACGTTGCCAGACGGTATCGAGGGTCAATTATCAGAGTTAAATGATGACGATGA
TGATGACGACTACGATGACGAAGACGACGAATCGAAATAA

Upstream 100 bases:

>100_bases
ACGCGGGTGAGCAGCTACTTGGTAACTACAAGTTGCGATTGCAATTCACGGCCTAATTTGGTATTATGTACTATAAACTG
TGGAAAGGACGTGCACCAGT

Downstream 100 bases:

>100_bases
GCTTTTCAAGATTTTTCCTTGACGAAAAGGGAATCTCCTTGTATTATCTTATTTGGGCTCTCTGGTAGAAATACCTGAGA
CAAACGATCGTATTAGCTCC

Product: DNA-directed RNA polymerase subunit delta

Products: diphosphate; RNAn+1

Alternate protein names: RNAP delta factor

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MELKQFDGQKKSELSLIEVAHAILSQHGDVMAFADLTNAVQSYLGKSDEEIRERLSQFYTDLNIDGSFISLGDNMWGLRA
WYPFESIDEAVIHTDDDEDEDRPKRKKVNAFLADAGDDDDVIDYDDDDPEDDDNYDDDDDQDDDTDDSASSKYDELAGVD
DTDDDVADETLPDGIEGQLSELNDDDDDDDYDDEDDESK

Sequences:

>Translated_199_residues
MELKQFDGQKKSELSLIEVAHAILSQHGDVMAFADLTNAVQSYLGKSDEEIRERLSQFYTDLNIDGSFISLGDNMWGLRA
WYPFESIDEAVIHTDDDEDEDRPKRKKVNAFLADAGDDDDVIDYDDDDPEDDDNYDDDDDQDDDTDDSASSKYDELAGVD
DTDDDVADETLPDGIEGQLSELNDDDDDDDYDDEDDESK
>Mature_199_residues
MELKQFDGQKKSELSLIEVAHAILSQHGDVMAFADLTNAVQSYLGKSDEEIRERLSQFYTDLNIDGSFISLGDNMWGLRA
WYPFESIDEAVIHTDDDEDEDRPKRKKVNAFLADAGDDDDVIDYDDDDPEDDDNYDDDDDQDDDTDDSASSKYDELAGVD
DTDDDVADETLPDGIEGQLSELNDDDDDDDYDDEDDESK

Specific function: Participates in both the initiation and recycling phases of transcription. In the presence of the delta subunit, RNAP displays an increased specificity of transcription, a decreased affinity for nucleic acids, and an increased efficiency of RNA synthesis

COG id: COG3343

COG function: function code K; DNA-directed RNA polymerase, delta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rpoE family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RPOE_LACPL (Q88Z77)

Other databases:

- EMBL:   AL935253
- RefSeq:   NP_784266.1
- GeneID:   1064139
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0480
- NMPDR:   fig|220668.1.peg.400
- HOGENOM:   HBG535252
- OMA:   TDMNTDG
- ProtClustDB:   PRK02363
- BioCyc:   LPLA220668:LP_0480-MONOMER
- HAMAP:   MF_00357
- InterPro:   IPR007759
- PANTHER:   PTHR23405:SF1

Pfam domain/function: PF05066 RNA_pol_delta

EC number: 2.7.7.6

Molecular weight: Translated: 22479; Mature: 22479

Theoretical pI: Translated: 3.45; Mature: 3.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKQFDGQKKSELSLIEVAHAILSQHGDVMAFADLTNAVQSYLGKSDEEIRERLSQFYT
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
DLNIDGSFISLGDNMWGLRAWYPFESIDEAVIHTDDDEDEDRPKRKKVNAFLADAGDDDD
CCCCCCCEEECCCCCCCCEEECCHHHHHHHHEECCCCCCCCHHHHHHHHHHHCCCCCCCC
VIDYDDDDPEDDDNYDDDDDQDDDTDDSASSKYDELAGVDDTDDDVADETLPDGIEGQLS
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCCCHHHH
ELNDDDDDDDYDDEDDESK
HCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MELKQFDGQKKSELSLIEVAHAILSQHGDVMAFADLTNAVQSYLGKSDEEIRERLSQFYT
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
DLNIDGSFISLGDNMWGLRAWYPFESIDEAVIHTDDDEDEDRPKRKKVNAFLADAGDDDD
CCCCCCCEEECCCCCCCCEEECCHHHHHHHHEECCCCCCCCHHHHHHHHHHHCCCCCCCC
VIDYDDDDPEDDDNYDDDDDQDDDTDDSASSKYDELAGVDDTDDDVADETLPDGIEGQLS
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCCCHHHH
ELNDDDDDDDYDDEDDESK
HCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nucleoside triphosphate; RNAn

Specific reaction: nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566