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The map label for this gene is pyrG

Identifier: 28377375

GI number: 28377375

Start: 429204

End: 430817

Strand: Direct

Name: pyrG

Synonym: lp_0481

Alternate gene names: 28377375

Gene position: 429204-430817 (Clockwise)

Preceding gene: 28377374

Following gene: 28377377

Centisome position: 12.97

GC content: 45.17

Gene sequence:

>1614_bases
ATGACCAAATATATTTTTGTAACTGGTGGCGTTGTGTCATCCATTGGTAAAGGTATCGTCGCTGCTTCGCTAGGGCGTTT
ATTGAAGAACCGGGGCTTAAAGGTCACGATTCAAAAGTTTGATCCCTATATCAACGTTGATCCTGGTACGATGAGTCCTT
ATCAACACGGTGAAGTCTTCGTGACCGATGATGGGACCGAAACTGACTTAGACCTTGGACATTATGAACGGTTTATCGAC
ATTAACCTTAATAAATATTCAAATGTTACCACCGGTAAGATTTATTCAGAAGTTCTGCAAAAGGAACGGCGGGGCGATTA
TTTAGGCGCCACGGTGCAAGTGATTCCGCATATCACGAACGCTATCAAGGAAAAAATCATGCGTGCGGGTACGACGACGG
ATTCCGATATCGTGATTACTGAAATCGGTGGGACGGTCGGTGATATCGAATCCTTGCCATTTATTGAAGCGCTACGGCAA
ATGAAGAGTGATTTAGGTTCCGACAATGTTTTCTATATCCATACCACATTGATCCCATATTTACGGGCAGCTGGTGAAAT
GAAGACGAAGCCAACGCAACATTCTGTTAAGGAATTGCGGAGTTATGGGATTCAGCCGAACATGTTAGTTGTCCGGACTG
AACAACCAATTACGCGGGAAATGCGGAATAAGATTGCGTCCTTCTGTGACGTGGAACCAGAAGCAGTCATTGAATCCTTA
GACGTTAAGACGATTTATTCAATTCCGTTGAATGTTCAGAAACAAAACATGGACCAAATCGTCCTTGACCATTTTGATGT
ACAGGCACCTAAGGCCGACATGAGTGAATGGATTGACTTAGAACATCATGTTCAGAACTTATCACGGACCATCAAGATTG
CGCTAGTCGGAAAATACGTCGCTTTACAGGATGCTTATATCTCAGTGACGGAAGCATTGAAGCATGCTGGCTATACGGAT
GATGCCGACATTGATTTGAAGAAGATTTCTGCTGAAGATGTTACGCCAGAAAATGTCGAAGAACTACTCGGCGATGCTGA
CGGAATCTTAGTTCCTGGTGGCTTTGGTGATCGGGGAATTGAAGGTAAGATTACGGCAATCAAGTATGCCCGTGAAAACG
ACGTGCCATTCTTAGGTATCTGCTTGGGAATGCAAATGGCCAGTGTCGAATTTGCACGTAACGTACTTGGATTGAAGGAT
GCTAACTCTGCTGAAATCGATCCGAAGACGCCGGACAATATTATTGATTTGATGGCCGACCAAGAAGACGTTGAAGACAT
GGGTGGAACGCAACGTTTAGGCGCTTACCCTTGCAAGCTGAAGCCGGGAACTGTGGCGGCTAAAGCCTATCACAATGAAG
AAGTTGTGATGGAACGTCATCGCCACCGTTATGAATTCAATAATAAGTATCGTGAAGCAATGGCTGCTAAGGGCATGGTC
TTCTCCGGAACTTCGCCTGACAACCGGCTCGTCGAAGTGATTGAATTACCAAAGAAGCGCTTCTTCGTGGCCTCACAATA
CCATCCAGAATTCTTATCACGGCCTAACCGTCCAGAAGGGTTATTCAAGGCATTCATCGATGCCGCTAACCAGACTGGTA
AGGTGAAGGCATAA

Upstream 100 bases:

>100_bases
ACCTGAGACAAACGATCGTATTAGCTCCCTATTTCTGCTGAAATAGGGAGCTTTCTTTATTTATACCACCCCACGAAATA
AATCTTTAAGGAGTTATCAC

Downstream 100 bases:

>100_bases
ATTCGAAATATAAGTGCTGTCAAAAAAACGCGTACTAGCTTGAATGGCTGGTACGCGTTTTTTGCGTGGTTATGGGGTGG
CTTTGAGCGACAATTTTCCG

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYINVDPGTMSPYQHGEVFVTDDGTETDLDLGHYERFID
INLNKYSNVTTGKIYSEVLQKERRGDYLGATVQVIPHITNAIKEKIMRAGTTTDSDIVITEIGGTVGDIESLPFIEALRQ
MKSDLGSDNVFYIHTTLIPYLRAAGEMKTKPTQHSVKELRSYGIQPNMLVVRTEQPITREMRNKIASFCDVEPEAVIESL
DVKTIYSIPLNVQKQNMDQIVLDHFDVQAPKADMSEWIDLEHHVQNLSRTIKIALVGKYVALQDAYISVTEALKHAGYTD
DADIDLKKISAEDVTPENVEELLGDADGILVPGGFGDRGIEGKITAIKYARENDVPFLGICLGMQMASVEFARNVLGLKD
ANSAEIDPKTPDNIIDLMADQEDVEDMGGTQRLGAYPCKLKPGTVAAKAYHNEEVVMERHRHRYEFNNKYREAMAAKGMV
FSGTSPDNRLVEVIELPKKRFFVASQYHPEFLSRPNRPEGLFKAFIDAANQTGKVKA

Sequences:

>Translated_537_residues
MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYINVDPGTMSPYQHGEVFVTDDGTETDLDLGHYERFID
INLNKYSNVTTGKIYSEVLQKERRGDYLGATVQVIPHITNAIKEKIMRAGTTTDSDIVITEIGGTVGDIESLPFIEALRQ
MKSDLGSDNVFYIHTTLIPYLRAAGEMKTKPTQHSVKELRSYGIQPNMLVVRTEQPITREMRNKIASFCDVEPEAVIESL
DVKTIYSIPLNVQKQNMDQIVLDHFDVQAPKADMSEWIDLEHHVQNLSRTIKIALVGKYVALQDAYISVTEALKHAGYTD
DADIDLKKISAEDVTPENVEELLGDADGILVPGGFGDRGIEGKITAIKYARENDVPFLGICLGMQMASVEFARNVLGLKD
ANSAEIDPKTPDNIIDLMADQEDVEDMGGTQRLGAYPCKLKPGTVAAKAYHNEEVVMERHRHRYEFNNKYREAMAAKGMV
FSGTSPDNRLVEVIELPKKRFFVASQYHPEFLSRPNRPEGLFKAFIDAANQTGKVKA
>Mature_536_residues
TKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYINVDPGTMSPYQHGEVFVTDDGTETDLDLGHYERFIDI
NLNKYSNVTTGKIYSEVLQKERRGDYLGATVQVIPHITNAIKEKIMRAGTTTDSDIVITEIGGTVGDIESLPFIEALRQM
KSDLGSDNVFYIHTTLIPYLRAAGEMKTKPTQHSVKELRSYGIQPNMLVVRTEQPITREMRNKIASFCDVEPEAVIESLD
VKTIYSIPLNVQKQNMDQIVLDHFDVQAPKADMSEWIDLEHHVQNLSRTIKIALVGKYVALQDAYISVTEALKHAGYTDD
ADIDLKKISAEDVTPENVEELLGDADGILVPGGFGDRGIEGKITAIKYARENDVPFLGICLGMQMASVEFARNVLGLKDA
NSAEIDPKTPDNIIDLMADQEDVEDMGGTQRLGAYPCKLKPGTVAAKAYHNEEVVMERHRHRYEFNNKYREAMAAKGMVF
SGTSPDNRLVEVIELPKKRFFVASQYHPEFLSRPNRPEGLFKAFIDAANQTGKVKA

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=547, Percent_Identity=47.1663619744059, Blast_Score=500, Evalue=1e-141,
Organism=Homo sapiens, GI28559085, Length=559, Percent_Identity=45.6171735241503, Blast_Score=476, Evalue=1e-134,
Organism=Homo sapiens, GI28559083, Length=559, Percent_Identity=45.6171735241503, Blast_Score=476, Evalue=1e-134,
Organism=Homo sapiens, GI221316689, Length=559, Percent_Identity=45.6171735241503, Blast_Score=476, Evalue=1e-134,
Organism=Escherichia coli, GI1789142, Length=546, Percent_Identity=52.5641025641026, Blast_Score=560, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI25148299, Length=603, Percent_Identity=38.4742951907131, Blast_Score=423, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6322563, Length=559, Percent_Identity=44.9016100178891, Blast_Score=483, Evalue=1e-137,
Organism=Saccharomyces cerevisiae, GI6319432, Length=557, Percent_Identity=44.524236983842, Blast_Score=476, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24664469, Length=553, Percent_Identity=46.6546112115732, Blast_Score=506, Evalue=1e-143,
Organism=Drosophila melanogaster, GI21357815, Length=498, Percent_Identity=44.578313253012, Blast_Score=428, Evalue=1e-120,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_LACPL (Q88Z76)

Other databases:

- EMBL:   AL935253
- RefSeq:   NP_784267.1
- ProteinModelPortal:   Q88Z76
- SMR:   Q88Z76
- GeneID:   1061431
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0481
- NMPDR:   fig|220668.1.peg.401
- HOGENOM:   HBG597806
- OMA:   IIHAGAN
- ProtClustDB:   PRK05380
- BioCyc:   LPLA220668:LP_0481-MONOMER
- BRENDA:   6.3.4.2
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 59737; Mature: 59606

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 381-381 ACT_SITE 508-508 ACT_SITE 510-510

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYINVDPGTMSPYQHGEVF
CCEEEEEECHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCCCCCEEE
VTDDGTETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLQKERRGDYLGATVQVIPHITN
EECCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AIKEKIMRAGTTTDSDIVITEIGGTVGDIESLPFIEALRQMKSDLGSDNVFYIHTTLIPY
HHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEHHHHHHH
LRAAGEMKTKPTQHSVKELRSYGIQPNMLVVRTEQPITREMRNKIASFCDVEPEAVIESL
HHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHC
DVKTIYSIPLNVQKQNMDQIVLDHFDVQAPKADMSEWIDLEHHVQNLSRTIKIALVGKYV
CCEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEEEEHHH
ALQDAYISVTEALKHAGYTDDADIDLKKISAEDVTPENVEELLGDADGILVPGGFGDRGI
HHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCHHHHHHHHCCCCCEEECCCCCCCCC
EGKITAIKYARENDVPFLGICLGMQMASVEFARNVLGLKDANSAEIDPKTPDNIIDLMAD
CCEEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCC
QEDVEDMGGTQRLGAYPCKLKPGTVAAKAYHNEEVVMERHRHRYEFNNKYREAMAAKGMV
HHHHHHCCCCCCCCCCCCCCCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCEE
FSGTSPDNRLVEVIELPKKRFFVASQYHPEFLSRPNRPEGLFKAFIDAANQTGKVKA
EECCCCCHHHHHHHHCCHHHEEEECCCCHHHHCCCCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYINVDPGTMSPYQHGEVF
CEEEEEECHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCCCCCEEE
VTDDGTETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLQKERRGDYLGATVQVIPHITN
EECCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AIKEKIMRAGTTTDSDIVITEIGGTVGDIESLPFIEALRQMKSDLGSDNVFYIHTTLIPY
HHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEHHHHHHH
LRAAGEMKTKPTQHSVKELRSYGIQPNMLVVRTEQPITREMRNKIASFCDVEPEAVIESL
HHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHC
DVKTIYSIPLNVQKQNMDQIVLDHFDVQAPKADMSEWIDLEHHVQNLSRTIKIALVGKYV
CCEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEEEEHHH
ALQDAYISVTEALKHAGYTDDADIDLKKISAEDVTPENVEELLGDADGILVPGGFGDRGI
HHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCHHHHHHHHCCCCCEEECCCCCCCCC
EGKITAIKYARENDVPFLGICLGMQMASVEFARNVLGLKDANSAEIDPKTPDNIIDLMAD
CCEEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCC
QEDVEDMGGTQRLGAYPCKLKPGTVAAKAYHNEEVVMERHRHRYEFNNKYREAMAAKGMV
HHHHHHCCCCCCCCCCCCCCCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCEE
FSGTSPDNRLVEVIELPKKRFFVASQYHPEFLSRPNRPEGLFKAFIDAANQTGKVKA
EECCCCCHHHHHHHHCCHHHEEEECCCCHHHHCCCCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566