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The map label for this gene is pbg1 [H]

Identifier: 28377342

GI number: 28377342

Start: 393593

End: 395029

Strand: Direct

Name: pbg1 [H]

Synonym: lp_0440

Alternate gene names: 28377342

Gene position: 393593-395029 (Clockwise)

Preceding gene: 28377341

Following gene: 28377343

Centisome position: 11.9

GC content: 41.54

Gene sequence:

>1437_bases
ATGACGATTAAAGGACGAGCGTTTCCAGAAGGGTTCTTATGGGGTGGCGCAGTGGCTGCACATCAACTCGAAGGTGGTTA
TAAAGAGGGCGGCAAGGGTCTCAGTACGGCAGATATTATGACATTAGGGACGAATGAGCGCCCTCGTGAAATTACGGACG
GAGTAGTGGCTGGCAAGTATTATCCTAATCATCAGGCAATTGATTTTTATCATCGTTATCCCGAAGATATTGAATTATTT
GCTGAAATGGGGTTTAAGTGTTTTCGAACATCGATTGCCTGGACGCGGATCTTCCCCAATGGTGATGAAAGCGAACCGAA
TGAAGCTGGATTACAATTTTATGATGATTTATTTGATGAATGCCTTAAAAATGGGATTCAACCAGTAGTGACACTAGCAC
ACTTTGAAATGCCATATCACTTAGTTAAGCAATACGGTGGGTGGCGTAATCGTAAGCTTATTCAATTCTATTTAAACTTT
GCAAAAGTATGTTTTGAACGTTATCGAGATAAAGTAACGTACTGGATGACTTTTAATGAAATCAACAATCAAACTAATTT
TGAATCAGACGGTGCCATGTTAACAGATTCTGGAATCATTCATCAACCAGGAGAAAACCGTGAACGCTGGATGTATCAGG
CGGCTCACTATGAACTGGTGGCGAGTGCCGCGGCGGTACAGTTAGGCCATCAGATTAATCCAGATTTTCAAATCGGTTGT
ATGATTGCGATGTGTCCGATCTATCCGCTTACAGCAGCACCAGCAGACGTCTTATTTGCGCAACGAGCGATGCAAACCCG
ATTTTATTTTGCCGATGTTCATTGCAATGGCACTTACCCACAATGGTTGCGTAATCGTTTTGAGTCGGAGCATTTTAACT
TGGACATTACGGCAGAAGATTTGAAAATCTTGCAAGCTGGAACGGTAGATTATATTGGATTTAGCTATTACATGTCGTTT
ACTGTGAAGGACACAGGAAAGCTAGCTTATAATGAAGAACATGATTTAGTTAAAAATCCATATGTTAAGGCTAGTGATTG
GGGCTGGCAAGTTGATCCCGTTGGGTTACGCTATGCTATGAATTGGTTCACGGATCGTTACCATTTGCCACTGTTTATCG
TGGAAAACGGTTTAGGCGCAATTGATAAGAAGACGGCAGATAACCAGATACATGACGATTATCGGATTGACTATTTAACG
GATCATTTACGACAAATTAAGCTGGCAGTTCTGGAAGATGGGGTTGATTTAATTGGGTATACGCCATGGGGGTGCATTGA
TTTAGTTGCGGCTAGTACTGGTCAAATGTCCAAACGCTATGGGTTTATCTATGTTGATGAGAACGATGATGGTTCAGGTA
GTCTAAAACGTTATAAAAAGGATTCATTTACTTGGTTTCAACATGTGATTGCGACAAATGGTGCCGAAATTGAGTAG

Upstream 100 bases:

>100_bases
TACTCGCTGCCAATAAGATGATGGCTGCAGATAGTCAATCTAAACAAGATACGGATACGACGACAGAAACGGTTAAAGCA
TAGTTTAAAAGGAGTTTAAC

Downstream 100 bases:

>100_bases
TGCGATTTGGGTATGACGATGGTCATTAATTATTGACTAGTGCTTAATTAATGATGTTTGAAATAGTTGTCAATGGCCAG
CTTTGATAGCTGGCCATTGG

Product: 6-phospho-beta-glucosidase

Products: NA

Alternate protein names: 6-phospho-beta-glucosidase [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MTIKGRAFPEGFLWGGAVAAHQLEGGYKEGGKGLSTADIMTLGTNERPREITDGVVAGKYYPNHQAIDFYHRYPEDIELF
AEMGFKCFRTSIAWTRIFPNGDESEPNEAGLQFYDDLFDECLKNGIQPVVTLAHFEMPYHLVKQYGGWRNRKLIQFYLNF
AKVCFERYRDKVTYWMTFNEINNQTNFESDGAMLTDSGIIHQPGENRERWMYQAAHYELVASAAAVQLGHQINPDFQIGC
MIAMCPIYPLTAAPADVLFAQRAMQTRFYFADVHCNGTYPQWLRNRFESEHFNLDITAEDLKILQAGTVDYIGFSYYMSF
TVKDTGKLAYNEEHDLVKNPYVKASDWGWQVDPVGLRYAMNWFTDRYHLPLFIVENGLGAIDKKTADNQIHDDYRIDYLT
DHLRQIKLAVLEDGVDLIGYTPWGCIDLVAASTGQMSKRYGFIYVDENDDGSGSLKRYKKDSFTWFQHVIATNGAEIE

Sequences:

>Translated_478_residues
MTIKGRAFPEGFLWGGAVAAHQLEGGYKEGGKGLSTADIMTLGTNERPREITDGVVAGKYYPNHQAIDFYHRYPEDIELF
AEMGFKCFRTSIAWTRIFPNGDESEPNEAGLQFYDDLFDECLKNGIQPVVTLAHFEMPYHLVKQYGGWRNRKLIQFYLNF
AKVCFERYRDKVTYWMTFNEINNQTNFESDGAMLTDSGIIHQPGENRERWMYQAAHYELVASAAAVQLGHQINPDFQIGC
MIAMCPIYPLTAAPADVLFAQRAMQTRFYFADVHCNGTYPQWLRNRFESEHFNLDITAEDLKILQAGTVDYIGFSYYMSF
TVKDTGKLAYNEEHDLVKNPYVKASDWGWQVDPVGLRYAMNWFTDRYHLPLFIVENGLGAIDKKTADNQIHDDYRIDYLT
DHLRQIKLAVLEDGVDLIGYTPWGCIDLVAASTGQMSKRYGFIYVDENDDGSGSLKRYKKDSFTWFQHVIATNGAEIE
>Mature_477_residues
TIKGRAFPEGFLWGGAVAAHQLEGGYKEGGKGLSTADIMTLGTNERPREITDGVVAGKYYPNHQAIDFYHRYPEDIELFA
EMGFKCFRTSIAWTRIFPNGDESEPNEAGLQFYDDLFDECLKNGIQPVVTLAHFEMPYHLVKQYGGWRNRKLIQFYLNFA
KVCFERYRDKVTYWMTFNEINNQTNFESDGAMLTDSGIIHQPGENRERWMYQAAHYELVASAAAVQLGHQINPDFQIGCM
IAMCPIYPLTAAPADVLFAQRAMQTRFYFADVHCNGTYPQWLRNRFESEHFNLDITAEDLKILQAGTVDYIGFSYYMSFT
VKDTGKLAYNEEHDLVKNPYVKASDWGWQVDPVGLRYAMNWFTDRYHLPLFIVENGLGAIDKKTADNQIHDDYRIDYLTD
HLRQIKLAVLEDGVDLIGYTPWGCIDLVAASTGQMSKRYGFIYVDENDDGSGSLKRYKKDSFTWFQHVIATNGAEIE

Specific function: Catalyzes the hydrolysis of aryl-phospho-beta-D- glucosides such as 4-methylumbelliferyl-phospho-beta-D- glucopyranoside (MUG-P), phosphoarbutin and phosphosalicin. Plays a major role in the utilization of arbutin or salicin as the sole carbon source. Bgl

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI110681710, Length=498, Percent_Identity=29.9196787148594, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI32481206, Length=495, Percent_Identity=29.2929292929293, Blast_Score=185, Evalue=7e-47,
Organism=Homo sapiens, GI13273313, Length=490, Percent_Identity=28.3673469387755, Blast_Score=168, Evalue=8e-42,
Organism=Homo sapiens, GI24497614, Length=485, Percent_Identity=29.6907216494845, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI28376633, Length=482, Percent_Identity=27.1784232365145, Blast_Score=148, Evalue=1e-35,
Organism=Escherichia coli, GI2367174, Length=479, Percent_Identity=57.8288100208768, Blast_Score=593, Evalue=1e-170,
Organism=Escherichia coli, GI1789070, Length=482, Percent_Identity=48.5477178423236, Blast_Score=482, Evalue=1e-137,
Organism=Escherichia coli, GI2367270, Length=478, Percent_Identity=50.836820083682, Blast_Score=473, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI17552856, Length=473, Percent_Identity=31.2896405919662, Blast_Score=188, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI17539390, Length=473, Percent_Identity=29.1754756871036, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI21356577, Length=500, Percent_Identity=28.8, Blast_Score=155, Evalue=5e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 54865; Mature: 54734

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1 ; PS00653 GLYCOSYL_HYDROL_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKGRAFPEGFLWGGAVAAHQLEGGYKEGGKGLSTADIMTLGTNERPREITDGVVAGKY
CCCCCCCCCCCCEECCHHHHHHHCCCHHCCCCCCCCEEEEEECCCCCCCHHHCCEEECCC
YPNHQAIDFYHRYPEDIELFAEMGFKCFRTSIAWTRIFPNGDESEPNEAGLQFYDDLFDE
CCCCCEEHHHHCCCHHHHHHHHHCHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHHHHHH
CLKNGIQPVVTLAHFEMPYHLVKQYGGWRNRKLIQFYLNFAKVCFERYRDKVTYWMTFNE
HHHCCCHHHEEHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEC
INNQTNFESDGAMLTDSGIIHQPGENRERWMYQAAHYELVASAAAVQLGHQINPDFQIGC
CCCCCCCCCCCCEEECCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
MIAMCPIYPLTAAPADVLFAQRAMQTRFYFADVHCNGTYPQWLRNRFESEHFNLDITAED
EEEECCCCCCCCCCHHHHHHHHHHHCEEEEEEEEECCCCHHHHHHHHCCCCEEEEEEHHH
LKILQAGTVDYIGFSYYMSFTVKDTGKLAYNEEHDLVKNPYVKASDWGWQVDPVGLRYAM
HHHHCCCCEEEEEEEEEEEEEEECCCCEEECCCCCHHCCCCEEECCCCEEECCCHHHHHH
NWFTDRYHLPLFIVENGLGAIDKKTADNQIHDDYRIDYLTDHLRQIKLAVLEDGVDLIGY
HHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEECC
TPWGCIDLVAASTGQMSKRYGFIYVDENDDGSGSLKRYKKDSFTWFQHVIATNGAEIE
CCCHHHHHHHCCCCCHHHCEEEEEEECCCCCCCHHHHHHCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TIKGRAFPEGFLWGGAVAAHQLEGGYKEGGKGLSTADIMTLGTNERPREITDGVVAGKY
CCCCCCCCCCCEECCHHHHHHHCCCHHCCCCCCCCEEEEEECCCCCCCHHHCCEEECCC
YPNHQAIDFYHRYPEDIELFAEMGFKCFRTSIAWTRIFPNGDESEPNEAGLQFYDDLFDE
CCCCCEEHHHHCCCHHHHHHHHHCHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHHHHHH
CLKNGIQPVVTLAHFEMPYHLVKQYGGWRNRKLIQFYLNFAKVCFERYRDKVTYWMTFNE
HHHCCCHHHEEHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEC
INNQTNFESDGAMLTDSGIIHQPGENRERWMYQAAHYELVASAAAVQLGHQINPDFQIGC
CCCCCCCCCCCCEEECCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
MIAMCPIYPLTAAPADVLFAQRAMQTRFYFADVHCNGTYPQWLRNRFESEHFNLDITAED
EEEECCCCCCCCCCHHHHHHHHHHHCEEEEEEEEECCCCHHHHHHHHCCCCEEEEEEHHH
LKILQAGTVDYIGFSYYMSFTVKDTGKLAYNEEHDLVKNPYVKASDWGWQVDPVGLRYAM
HHHHCCCCEEEEEEEEEEEEEEECCCCEEECCCCCHHCCCCEEECCCCEEECCCHHHHHH
NWFTDRYHLPLFIVENGLGAIDKKTADNQIHDDYRIDYLTDHLRQIKLAVLEDGVDLIGY
HHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEECC
TPWGCIDLVAASTGQMSKRYGFIYVDENDDGSGSLKRYKKDSFTWFQHVIATNGAEIE
CCCHHHHHHHCCCCCHHHCEEEEEEECCCCCCCHHHHHHCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8125345; 9384377 [H]