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The map label for this gene is pts8C [H]

Identifier: 28377341

GI number: 28377341

Start: 392220

End: 393575

Strand: Direct

Name: pts8C [H]

Synonym: lp_0439

Alternate gene names: 28377341

Gene position: 392220-393575 (Clockwise)

Preceding gene: 28377340

Following gene: 28377342

Centisome position: 11.86

GC content: 39.6

Gene sequence:

>1356_bases
ATGAACGGTTTTAACGTGTTTATGGAAAAACACATTGTACCATTTGCAGTCAAGTTGAATGAACAGCGACATGTCGCGGC
AATCCGTGATGCTTTTATGTACACATTCCCAATTACAATGGCTGCATCGTTAGTTATTTTAATTAATAACTTAGTTTTTT
CAAAAACGGGGTTTATTGCACAAATCTTATTTTTACCCAAATTTTTTCCACACTTGGAAAATGCCCAAAAGTTATTAACC
TCAGTAACTAACGGAACGATGAATATTTTATCGATTTTTATTGCGTATCTGGTGGCACGGAATTTAGCCAAATATTTTAA
AGCTGATGACATGTTAGTAGGGATGACGGGGATTGCCTGCTTCATGATTTTATACTCACCATCAATTATCAAAGACGGTG
TAACCTATTTGCCAACGACTTATTTAGGTGCACAAGGGCTGTTTGTTGCAATGATAGTAGGTGGCTTAGTTGGAGAATTT
TTACCACGGCTAACGCGCATTAAGAAAATGCAAATCAAAATGCCTGAGATGGTTCCGCCGGCAGTTGCACGGTCTTTCAA
TGGCTTAATTCCTATCGTTATTATGATTATGCTGTTTTCTATGTTGAACTATGGGTTATCACTAATTTCACCACAAGGAA
TCAATGATATTATTTATAAATCAATTCAAACACCATTAACCCACATTGGGGTCAACTTATTTGGCGTTATCATTATTGCT
ATTGTTCAAAATCTTTTGTGGCTTGTCGGTATCCATGGTCCAAACACGTTGAACGCGTTACGATCAATTATTTTTACTGA
AGCTGATTTGAAAAACCAAACGTTTATTAACGCTCATGGTACAGCTTGGGGTGTGCCCAACCGTGCAACCTGGAGTGTGT
TGAATGATGTCTTCGCCAATATGGGTGGTAGTGGGATGACACTTGGCTTGATTATTGCTATCTTCCTCGTCTCAAGGCGT
AAAGATTATCGTGAGATTGCTAAATTGGCATTAGTGCCCGGCCTCTTCAATATTAACGAACCGCTAATGTTCGGTTTACC
AATTGTTTTGAATCCAATTATGGCGATTCCATTCGTTCTAACACCAGTGATCAATATCTTGGTTGGCTATACAGTGACAG
TTATTTTTAACTGGATACCGACGCCGGCTTTTGGATTGACATGGACAACACCGGGACCACTGATGCCGTTCTTGGGGACT
GGTGGCAATTGGCTTGGCCTGATTATTGGATTTGTTTGTTTGGGAATTTCCGTGTTGACGTACATGCCATTTGTACTCGC
TGCCAATAAGATGATGGCTGCAGATAGTCAATCTAAACAAGATACGGATACGACGACAGAAACGGTTAAAGCATAG

Upstream 100 bases:

>100_bases
ATGGAACAAGTGAGCAACTGCATTGTAAGCGCTTTTAAAACGCATGTAGTTCACTTATACTAGCTTTTGTAAGTTAAATC
AAAGGGAGGAACAACTTATG

Downstream 100 bases:

>100_bases
TTTAAAAGGAGTTTAACATGACGATTAAAGGACGAGCGTTTCCAGAAGGGTTCTTATGGGGTGGCGCAGTGGCTGCACAT
CAACTCGAAGGTGGTTATAA

Product: cellobiose PTS, EIIC

Products: protein histidine; sugar phosphate

Alternate protein names: EIIC-Chb; PTS system N,N'-diacetylchitobiose-specific EIIC component [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MNGFNVFMEKHIVPFAVKLNEQRHVAAIRDAFMYTFPITMAASLVILINNLVFSKTGFIAQILFLPKFFPHLENAQKLLT
SVTNGTMNILSIFIAYLVARNLAKYFKADDMLVGMTGIACFMILYSPSIIKDGVTYLPTTYLGAQGLFVAMIVGGLVGEF
LPRLTRIKKMQIKMPEMVPPAVARSFNGLIPIVIMIMLFSMLNYGLSLISPQGINDIIYKSIQTPLTHIGVNLFGVIIIA
IVQNLLWLVGIHGPNTLNALRSIIFTEADLKNQTFINAHGTAWGVPNRATWSVLNDVFANMGGSGMTLGLIIAIFLVSRR
KDYREIAKLALVPGLFNINEPLMFGLPIVLNPIMAIPFVLTPVINILVGYTVTVIFNWIPTPAFGLTWTTPGPLMPFLGT
GGNWLGLIIGFVCLGISVLTYMPFVLAANKMMAADSQSKQDTDTTTETVKA

Sequences:

>Translated_451_residues
MNGFNVFMEKHIVPFAVKLNEQRHVAAIRDAFMYTFPITMAASLVILINNLVFSKTGFIAQILFLPKFFPHLENAQKLLT
SVTNGTMNILSIFIAYLVARNLAKYFKADDMLVGMTGIACFMILYSPSIIKDGVTYLPTTYLGAQGLFVAMIVGGLVGEF
LPRLTRIKKMQIKMPEMVPPAVARSFNGLIPIVIMIMLFSMLNYGLSLISPQGINDIIYKSIQTPLTHIGVNLFGVIIIA
IVQNLLWLVGIHGPNTLNALRSIIFTEADLKNQTFINAHGTAWGVPNRATWSVLNDVFANMGGSGMTLGLIIAIFLVSRR
KDYREIAKLALVPGLFNINEPLMFGLPIVLNPIMAIPFVLTPVINILVGYTVTVIFNWIPTPAFGLTWTTPGPLMPFLGT
GGNWLGLIIGFVCLGISVLTYMPFVLAANKMMAADSQSKQDTDTTTETVKA
>Mature_451_residues
MNGFNVFMEKHIVPFAVKLNEQRHVAAIRDAFMYTFPITMAASLVILINNLVFSKTGFIAQILFLPKFFPHLENAQKLLT
SVTNGTMNILSIFIAYLVARNLAKYFKADDMLVGMTGIACFMILYSPSIIKDGVTYLPTTYLGAQGLFVAMIVGGLVGEF
LPRLTRIKKMQIKMPEMVPPAVARSFNGLIPIVIMIMLFSMLNYGLSLISPQGINDIIYKSIQTPLTHIGVNLFGVIIIA
IVQNLLWLVGIHGPNTLNALRSIIFTEADLKNQTFINAHGTAWGVPNRATWSVLNDVFANMGGSGMTLGLIIAIFLVSRR
KDYREIAKLALVPGLFNINEPLMFGLPIVLNPIMAIPFVLTPVINILVGYTVTVIFNWIPTPAFGLTWTTPGPLMPFLGT
GGNWLGLIIGFVCLGISVLTYMPFVLAANKMMAADSQSKQDTDTTTETVKA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=454, Percent_Identity=35.2422907488987, Blast_Score=229, Evalue=2e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003352
- InterPro:   IPR004796
- InterPro:   IPR004501 [H]

Pfam domain/function: PF02378 PTS_EIIC [H]

EC number: 2.7.1.69

Molecular weight: Translated: 49515; Mature: 49515

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGFNVFMEKHIVPFAVKLNEQRHVAAIRDAFMYTFPITMAASLVILINNLVFSKTGFIA
CCCHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QILFLPKFFPHLENAQKLLTSVTNGTMNILSIFIAYLVARNLAKYFKADDMLVGMTGIAC
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHH
FMILYSPSIIKDGVTYLPTTYLGAQGLFVAMIVGGLVGEFLPRLTRIKKMQIKMPEMVPP
HHHHHCCHHHHCCCHHCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
AVARSFNGLIPIVIMIMLFSMLNYGLSLISPQGINDIIYKSIQTPLTHIGVNLFGVIIIA
HHHHHCCCHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHH
IVQNLLWLVGIHGPNTLNALRSIIFTEADLKNQTFINAHGTAWGVPNRATWSVLNDVFAN
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHC
MGGSGMTLGLIIAIFLVSRRKDYREIAKLALVPGLFNINEPLMFGLPIVLNPIMAIPFVL
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHH
TPVINILVGYTVTVIFNWIPTPAFGLTWTTPGPLMPFLGTGGNWLGLIIGFVCLGISVLT
HHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
YMPFVLAANKMMAADSQSKQDTDTTTETVKA
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHCC
>Mature Secondary Structure
MNGFNVFMEKHIVPFAVKLNEQRHVAAIRDAFMYTFPITMAASLVILINNLVFSKTGFIA
CCCHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QILFLPKFFPHLENAQKLLTSVTNGTMNILSIFIAYLVARNLAKYFKADDMLVGMTGIAC
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHH
FMILYSPSIIKDGVTYLPTTYLGAQGLFVAMIVGGLVGEFLPRLTRIKKMQIKMPEMVPP
HHHHHCCHHHHCCCHHCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
AVARSFNGLIPIVIMIMLFSMLNYGLSLISPQGINDIIYKSIQTPLTHIGVNLFGVIIIA
HHHHHCCCHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHH
IVQNLLWLVGIHGPNTLNALRSIIFTEADLKNQTFINAHGTAWGVPNRATWSVLNDVFAN
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHC
MGGSGMTLGLIIAIFLVSRRKDYREIAKLALVPGLFNINEPLMFGLPIVLNPIMAIPFVL
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHH
TPVINILVGYTVTVIFNWIPTPAFGLTWTTPGPLMPFLGTGGNWLGLIIGFVCLGISVLT
HHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
YMPFVLAANKMMAADSQSKQDTDTTTETVKA
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2179047; 9097039; 9278503; 2092358; 9405618; 10913117 [H]