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The map label for this gene is malE [H]

Identifier: 28377115

GI number: 28377115

Start: 151222

End: 152475

Strand: Direct

Name: malE [H]

Synonym: lp_0175

Alternate gene names: 28377115

Gene position: 151222-152475 (Clockwise)

Preceding gene: 28377114

Following gene: 28377116

Centisome position: 4.57

GC content: 42.11

Gene sequence:

>1254_bases
ATGAGTACTTGGAAAAAGTTGGGGATGGGTGCCCTAGCCACTGGTTTAGCACTCACGCTCGTAGGTTGTGGTAATAGTAA
ATCTTCATCTAGTAGTTCAAAGATGGACAAGACGTTAAAGGTTTCAGCGGATCCTTCATATAAGTCATACATGAAATCGG
TGATTCCAAAGTTTGAAAAGAAGTACAACGTTAAGGTTAAGGTTTCATACAAGGCCATGCTTGATGAAGACGATGCTTTG
AAGCTGGATGGTCCTTCCGGCAAGGGTCCTGATGTTTTAATGGCACCATACGACCGGGTTGGTCAAATGGCGTCACAAGG
TCAACTATCAACAGTCAAGTTGACCAGTGGTCGTTACAACGCAACGGGGAAGAAATCTGTTACTTATAAGAATAAGGTTT
ATGCAGCGCCTGTAACGATCGAATCTGACGTTCTGTACTACAACAAGAAGCTAATCAAGACTGCACCTAAGAACTTCAAG
GAACTTGAAAAGTTAGCTTCTGACAAGAAGTATGCTTACGCTAATGATAAGACTAAGTCAGTTGCCTTCTTGACTCAATG
GACAAACTTCTACAATGCCTTTGGTGTCATCAAAGGTTATGGTGGTTACGTCTTTGCTGATGGCAATACGAACGCCAAGA
AGATGGGCTTGAACAATTCTGGCGCCGTTGAAGGTTTAACTTACATGACGAAGTGGTTTACGAAGTACTGGCCAAGTGGG
ATGCAAAATGTCACTTCTAACGAAAACTTTGTGACCTCACAATTTACGTCTAATAAGACTGCTGCTGTTATTGATGGCCC
TTGGATGGCTGCAACTTACAAGAAGTCTAATATCGATTATGGTGTTGCTGTTTTACCTAAGTTGAATAACGGCAAAGATT
ATCAAGCGTTTGCCGGTGGTAAAGCTTGGGCAATCTCAAACTACTCTAAGCATAAGACTGCTGCACAAGCTTGGTTAGAC
TTCATCTCTAACAAGACTAACCAAAACAAGCTTTACAAGTCTGCTAGTGAAATTCCAGCAAACACAGCTGCTCGTAAGGA
AGCTGTAAGTACTGGCGATGACATGGCTACGGCCGTTTCAAAGCAATACGACAAGGATGTTCCTTTGATCAACTTACCAC
AAATGTCAGAAGTTTGGACACCAGCACAAACGATGGTTACCAATGATGCTAGCGGCAAGATGACACCAAAGCAAGCTGCT
AATAAAGCGCAAAAGACGATCTTAACTAATATCTCACAAAAGTATGATAAGTAA

Upstream 100 bases:

>100_bases
TGTTAACGTTAACATTACGAAAACGCTTGCGAAAGCGTTTGCACAGTTGCATAATAAATTATGGTAAATGAATTAAGATA
CTTCGCAAGGAGGAAGCACT

Downstream 100 bases:

>100_bases
TTAAAATTGTAATTTAAGGGGGCTGAAACAAAATTCGTTTTGTCTCAGCCTTTTTAAAAGGAAAAATGCACGATATTTAG
AATTTATTTAGGAAAGGGGT

Product: maltose/maltodextrin ABC transporter, substrate binding protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MSTWKKLGMGALATGLALTLVGCGNSKSSSSSSKMDKTLKVSADPSYKSYMKSVIPKFEKKYNVKVKVSYKAMLDEDDAL
KLDGPSGKGPDVLMAPYDRVGQMASQGQLSTVKLTSGRYNATGKKSVTYKNKVYAAPVTIESDVLYYNKKLIKTAPKNFK
ELEKLASDKKYAYANDKTKSVAFLTQWTNFYNAFGVIKGYGGYVFADGNTNAKKMGLNNSGAVEGLTYMTKWFTKYWPSG
MQNVTSNENFVTSQFTSNKTAAVIDGPWMAATYKKSNIDYGVAVLPKLNNGKDYQAFAGGKAWAISNYSKHKTAAQAWLD
FISNKTNQNKLYKSASEIPANTAARKEAVSTGDDMATAVSKQYDKDVPLINLPQMSEVWTPAQTMVTNDASGKMTPKQAA
NKAQKTILTNISQKYDK

Sequences:

>Translated_417_residues
MSTWKKLGMGALATGLALTLVGCGNSKSSSSSSKMDKTLKVSADPSYKSYMKSVIPKFEKKYNVKVKVSYKAMLDEDDAL
KLDGPSGKGPDVLMAPYDRVGQMASQGQLSTVKLTSGRYNATGKKSVTYKNKVYAAPVTIESDVLYYNKKLIKTAPKNFK
ELEKLASDKKYAYANDKTKSVAFLTQWTNFYNAFGVIKGYGGYVFADGNTNAKKMGLNNSGAVEGLTYMTKWFTKYWPSG
MQNVTSNENFVTSQFTSNKTAAVIDGPWMAATYKKSNIDYGVAVLPKLNNGKDYQAFAGGKAWAISNYSKHKTAAQAWLD
FISNKTNQNKLYKSASEIPANTAARKEAVSTGDDMATAVSKQYDKDVPLINLPQMSEVWTPAQTMVTNDASGKMTPKQAA
NKAQKTILTNISQKYDK
>Mature_416_residues
STWKKLGMGALATGLALTLVGCGNSKSSSSSSKMDKTLKVSADPSYKSYMKSVIPKFEKKYNVKVKVSYKAMLDEDDALK
LDGPSGKGPDVLMAPYDRVGQMASQGQLSTVKLTSGRYNATGKKSVTYKNKVYAAPVTIESDVLYYNKKLIKTAPKNFKE
LEKLASDKKYAYANDKTKSVAFLTQWTNFYNAFGVIKGYGGYVFADGNTNAKKMGLNNSGAVEGLTYMTKWFTKYWPSGM
QNVTSNENFVTSQFTSNKTAAVIDGPWMAATYKKSNIDYGVAVLPKLNNGKDYQAFAGGKAWAISNYSKHKTAAQAWLDF
ISNKTNQNKLYKSASEIPANTAARKEAVSTGDDMATAVSKQYDKDVPLINLPQMSEVWTPAQTMVTNDASGKMTPKQAAN
KAQKTILTNISQKYDK

Specific function: Part of the ABC transporter complex involved in maltodextrin import. Binds maltodextrin. Can also bind maltose with low affinity, but is not involved in its uptake [H]

COG id: COG2182

COG function: function code G; Maltose-binding periplasmic proteins/domains

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790466, Length=391, Percent_Identity=27.8772378516624, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 45668; Mature: 45537

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTWKKLGMGALATGLALTLVGCGNSKSSSSSSKMDKTLKVSADPSYKSYMKSVIPKFEK
CCCHHHHCCHHHHHCEEEEEEECCCCCCCCCHHHCCCEEEEECCCCHHHHHHHHHHHHHH
KYNVKVKVSYKAMLDEDDALKLDGPSGKGPDVLMAPYDRVGQMASQGQLSTVKLTSGRYN
CCCEEEEEEEEEECCCCCCEEECCCCCCCCCEEECCHHHHHHHHCCCCCEEEEEECCCCC
ATGKKSVTYKNKVYAAPVTIESDVLYYNKKLIKTAPKNFKELEKLASDKKYAYANDKTKS
CCCCCCEEECCEEEECCEEEECCCHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCCCCE
VAFLTQWTNFYNAFGVIKGYGGYVFADGNTNAKKMGLNNSGAVEGLTYMTKWFTKYWPSG
EEEHHHHHHHHHHHHHEECCCCEEEECCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCH
MQNVTSNENFVTSQFTSNKTAAVIDGPWMAATYKKSNIDYGVAVLPKLNNGKDYQAFAGG
HHHCCCCCCEEEEECCCCCEEEEEECCEEEEEEECCCCCCCEEEEEECCCCCCCEEECCC
KAWAISNYSKHKTAAQAWLDFISNKTNQNKLYKSASEIPANTAARKEAVSTGDDMATAVS
CEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHH
KQYDKDVPLINLPQMSEVWTPAQTMVTNDASGKMTPKQAANKAQKTILTNISQKYDK
HHHCCCCCEEECCCHHHHCCCHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STWKKLGMGALATGLALTLVGCGNSKSSSSSSKMDKTLKVSADPSYKSYMKSVIPKFEK
CCHHHHCCHHHHHCEEEEEEECCCCCCCCCHHHCCCEEEEECCCCHHHHHHHHHHHHHH
KYNVKVKVSYKAMLDEDDALKLDGPSGKGPDVLMAPYDRVGQMASQGQLSTVKLTSGRYN
CCCEEEEEEEEEECCCCCCEEECCCCCCCCCEEECCHHHHHHHHCCCCCEEEEEECCCCC
ATGKKSVTYKNKVYAAPVTIESDVLYYNKKLIKTAPKNFKELEKLASDKKYAYANDKTKS
CCCCCCEEECCEEEECCEEEECCCHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCCCCE
VAFLTQWTNFYNAFGVIKGYGGYVFADGNTNAKKMGLNNSGAVEGLTYMTKWFTKYWPSG
EEEHHHHHHHHHHHHHEECCCCEEEECCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCH
MQNVTSNENFVTSQFTSNKTAAVIDGPWMAATYKKSNIDYGVAVLPKLNNGKDYQAFAGG
HHHCCCCCCEEEEECCCCCEEEEEECCEEEEEEECCCCCCCEEEEEECCCCCCCEEECCC
KAWAISNYSKHKTAAQAWLDFISNKTNQNKLYKSASEIPANTAARKEAVSTGDDMATAVS
CEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHH
KQYDKDVPLINLPQMSEVWTPAQTMVTNDASGKMTPKQAANKAQKTILTNISQKYDK
HHHCCCCCEEECCCHHHHCCCHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]