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The map label for this gene is malF [H]

Identifier: 28377116

GI number: 28377116

Start: 152585

End: 153874

Strand: Direct

Name: malF [H]

Synonym: lp_0176

Alternate gene names: 28377116

Gene position: 152585-153874 (Clockwise)

Preceding gene: 28377115

Following gene: 28377117

Centisome position: 4.61

GC content: 41.71

Gene sequence:

>1290_bases
ATGCAAGTCAAGGATGTAAAGAAAGCGCAACGCCTGTCAATTATTCCCGGGCTCGGTCAGCTGTATAACGGTCAGACCAT
AAAAGGTGTTGGCTTCATTATTATTCTATTGGCATTTATTGCTGAGTTCATCTTCGGGGGCTTTAGCGCATTTGCCAATT
TAGTCACATTGGGTTCAGTCTCAATGGAAGACAACTCTTTGTTCATGATGATCTATGGGACGTTGCAGATTCTATTGACA
ATTGTGTTTATCGTATTTTATATTGCAAACCTTTACGATGCGAAACACGTTGCACAGTTAATTAATAATGGTAAAAAGGT
CTCCCATACGTTTAAAGAGTTAGGTGCGAGTTTAGTAGATGGTGGCTTTGCTTATTTATTAACCACACCGGCCTATATTT
TAATGGTTTTTACGATTGTGTTCCCAGTATTGGTTACACTACTGATGGCTTTTACGAACTATGATTTTGCGCATATTCCA
CCAGCTATGTTATTGGATTGGATTGGGTTCAAAAACTTCACATCAATGTTCTTCTTGAGTAGTTACCGGGCAACCTTCAA
CGCTGTTTTTGGTTGGACCGTTATTTGGACCGTATGTGCAACCACGTTACAAATTATTATTGGGATTGCCACCGCAGTAA
TTGCTAATCAAAACTTCATCAAAGGTAAGCGCTTCTTCGGAGTGATTTTCTTGCTTCCTTGGGCGATTCCAGCGTTTGTA
TCAATTATGAGTTTTTCAAACATTTTTAATGATTCAGCTGGTGCGATCAATGTGCAGGTCATCGGCTTATGGAATACGTT
AGTTCCATTTGCTCACATTGCGCCGATCCACTGGATGACGGATACGTTCTGGACTAAAGTTGCTATTATTATGATTCAAG
GTTGGTTAGGATTCCCGTACATTTACGTTATGATTACTGGTATACTGCAAGCGATTCCAGAAGATCTCTACGAAGCCGCA
ACGCTAGATGGTGCGACAGTAATGCAAAAGTTCAACAAGATTACGTTGCCAACGATTTTTGCTATTGCAGCGCCAATCTT
TGTGACGCAGTATACGTTTAACTTCAATAACTTCTCGATGATCTATCTGTTTAACCAAGGTGGCCCTGGTGATGTCGGTG
CTAACGCCGGTGGGACGGATATCCTGATCTCATGGATCTACAAGTTAACAACGGGGACGTCACCACAGTATTCATTGGCC
GCGGCCGTTACGTTGATTATTTCCGTCTTAGTTATCGGGATCTCATTGATTGTCTTCAAGAAGACCCACGCGTTTGAAAT
GGGGGACTAG

Upstream 100 bases:

>100_bases
TAATTTAAGGGGGCTGAAACAAAATTCGTTTTGTCTCAGCCTTTTTAAAAGGAAAAATGCACGATATTTAGAATTTATTT
AGGAAAGGGGTGCGGGCGAC

Downstream 100 bases:

>100_bases
TGTTATGGATAATGCACAAGCAGTGGCACCTAAGAATGCCAATTCAAAGCAAAATAGCGCGCGTCGGCACCGTTTCTGGA
AAGAATTCTTTACGTATCTG

Product: maltose/maltodextrin ABC transporter, permease protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MQVKDVKKAQRLSIIPGLGQLYNGQTIKGVGFIIILLAFIAEFIFGGFSAFANLVTLGSVSMEDNSLFMMIYGTLQILLT
IVFIVFYIANLYDAKHVAQLINNGKKVSHTFKELGASLVDGGFAYLLTTPAYILMVFTIVFPVLVTLLMAFTNYDFAHIP
PAMLLDWIGFKNFTSMFFLSSYRATFNAVFGWTVIWTVCATTLQIIIGIATAVIANQNFIKGKRFFGVIFLLPWAIPAFV
SIMSFSNIFNDSAGAINVQVIGLWNTLVPFAHIAPIHWMTDTFWTKVAIIMIQGWLGFPYIYVMITGILQAIPEDLYEAA
TLDGATVMQKFNKITLPTIFAIAAPIFVTQYTFNFNNFSMIYLFNQGGPGDVGANAGGTDILISWIYKLTTGTSPQYSLA
AAVTLIISVLVIGISLIVFKKTHAFEMGD

Sequences:

>Translated_429_residues
MQVKDVKKAQRLSIIPGLGQLYNGQTIKGVGFIIILLAFIAEFIFGGFSAFANLVTLGSVSMEDNSLFMMIYGTLQILLT
IVFIVFYIANLYDAKHVAQLINNGKKVSHTFKELGASLVDGGFAYLLTTPAYILMVFTIVFPVLVTLLMAFTNYDFAHIP
PAMLLDWIGFKNFTSMFFLSSYRATFNAVFGWTVIWTVCATTLQIIIGIATAVIANQNFIKGKRFFGVIFLLPWAIPAFV
SIMSFSNIFNDSAGAINVQVIGLWNTLVPFAHIAPIHWMTDTFWTKVAIIMIQGWLGFPYIYVMITGILQAIPEDLYEAA
TLDGATVMQKFNKITLPTIFAIAAPIFVTQYTFNFNNFSMIYLFNQGGPGDVGANAGGTDILISWIYKLTTGTSPQYSLA
AAVTLIISVLVIGISLIVFKKTHAFEMGD
>Mature_429_residues
MQVKDVKKAQRLSIIPGLGQLYNGQTIKGVGFIIILLAFIAEFIFGGFSAFANLVTLGSVSMEDNSLFMMIYGTLQILLT
IVFIVFYIANLYDAKHVAQLINNGKKVSHTFKELGASLVDGGFAYLLTTPAYILMVFTIVFPVLVTLLMAFTNYDFAHIP
PAMLLDWIGFKNFTSMFFLSSYRATFNAVFGWTVIWTVCATTLQIIIGIATAVIANQNFIKGKRFFGVIFLLPWAIPAFV
SIMSFSNIFNDSAGAINVQVIGLWNTLVPFAHIAPIHWMTDTFWTKVAIIMIQGWLGFPYIYVMITGILQAIPEDLYEAA
TLDGATVMQKFNKITLPTIFAIAAPIFVTQYTFNFNNFSMIYLFNQGGPGDVGANAGGTDILISWIYKLTTGTSPQYSLA
AAVTLIISVLVIGISLIVFKKTHAFEMGD

Specific function: Part of the ABC transporter complex involved in maltodextrin import. Probably responsible for the translocation of the substrate across the membrane (Probable) [H]

COG id: COG1175

COG function: function code G; ABC-type sugar transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1790465, Length=259, Percent_Identity=35.1351351351351, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1787570, Length=233, Percent_Identity=24.8927038626609, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 47331; Mature: 47331

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCC
>Mature Secondary Structure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CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]