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The map label for this gene is proB

Identifier: 28376988

GI number: 28376988

Start: 16629

End: 17435

Strand: Direct

Name: proB

Synonym: lp_0016

Alternate gene names: 28376988

Gene position: 16629-17435 (Clockwise)

Preceding gene: 28376987

Following gene: 28376989

Centisome position: 0.5

GC content: 43.62

Gene sequence:

>807_bases
GTGGCAGTAGTGAAAGTGATACGAAAACTAAAGGCTAAACGAGTCGTGATTAAAGTCGGGACAAGCACGCTAGTTTATCC
AGATGGATCGATCAACTTACGGGCAATCGATCAATTAGCCTTTGTCATTAGCGCAATGCGACACCGTGGGCGTGAAGTTG
TCTTGGTTTCGTCAGGGGCTATTGGTGTTGGCTTGAATTATATGGGACTCAAGCAACGTCCAAAAGCAATTCCTGAACAA
CAGGCGATTGCGGCGATTGGGCAAACTGAATTGATTTCGATTTACAAAGAACGTTTCGCCATTTATGAACAAAAAATTGG
CCAGTTGTTATTAACACGTGATATCTTTGTTTATCCTAAGAGTCACCATAATGTGTTGAATACATTTGAGGCGCTATTAA
AACAAAACGTGGTCCCAATTGTTAATGAAAATGATACGGTCGCGGTCGATGAATTGGATCATGAAACAAAGTTCGGTGAT
AACGATCAACTATCCGCAATCGTGGCCACGAATATCGGGGCGGATTTGTTAATCATGCTATCCGACATCGATGGCTTTTA
TGATGGTAATCCCAATGCGGATGCCCGAGCCCAGTTATTAGGGCACATTGAGAAGGTTGATCAACATACTTATAAGCTAG
CTGGCGGTAGTGGCAGTCGGTTTGGTACTGGTGGGATGGTCACTAAGCTAAAAGCGGCAGAACGGATGATCGATGCGAAT
GGTCAAATGATTTTAGCCAATGGTGCGGATCCCACAATTATTTTTCAAATTTTAGCAGGCGAACCAGTCGGAACGCTATT
CGGTTAA

Upstream 100 bases:

>100_bases
TATCGGTGTCGTTCACTTTAATTTACGCAAGGCCGTTAATTATATGAAGACGGACGTTCAGACCGATGTTTCACGTGAAA
CAAAATAATTAGAGAAGTTG

Downstream 100 bases:

>100_bases
GTTGGGGAGGTTATCAAAATGGTAGATTTAGAACAGTTGGGTCAGCAGGCTCAACAAGCTAGCTATACGCTGGGGTTACT
TTCAACGGCGCAAAAAAATC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MAVVKVIRKLKAKRVVIKVGTSTLVYPDGSINLRAIDQLAFVISAMRHRGREVVLVSSGAIGVGLNYMGLKQRPKAIPEQ
QAIAAIGQTELISIYKERFAIYEQKIGQLLLTRDIFVYPKSHHNVLNTFEALLKQNVVPIVNENDTVAVDELDHETKFGD
NDQLSAIVATNIGADLLIMLSDIDGFYDGNPNADARAQLLGHIEKVDQHTYKLAGGSGSRFGTGGMVTKLKAAERMIDAN
GQMILANGADPTIIFQILAGEPVGTLFG

Sequences:

>Translated_268_residues
MAVVKVIRKLKAKRVVIKVGTSTLVYPDGSINLRAIDQLAFVISAMRHRGREVVLVSSGAIGVGLNYMGLKQRPKAIPEQ
QAIAAIGQTELISIYKERFAIYEQKIGQLLLTRDIFVYPKSHHNVLNTFEALLKQNVVPIVNENDTVAVDELDHETKFGD
NDQLSAIVATNIGADLLIMLSDIDGFYDGNPNADARAQLLGHIEKVDQHTYKLAGGSGSRFGTGGMVTKLKAAERMIDAN
GQMILANGADPTIIFQILAGEPVGTLFG
>Mature_267_residues
AVVKVIRKLKAKRVVIKVGTSTLVYPDGSINLRAIDQLAFVISAMRHRGREVVLVSSGAIGVGLNYMGLKQRPKAIPEQQ
AIAAIGQTELISIYKERFAIYEQKIGQLLLTRDIFVYPKSHHNVLNTFEALLKQNVVPIVNENDTVAVDELDHETKFGDN
DQLSAIVATNIGADLLIMLSDIDGFYDGNPNADARAQLLGHIEKVDQHTYKLAGGSGSRFGTGGMVTKLKAAERMIDANG
QMILANGADPTIIFQILAGEPVGTLFG

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate 5-kinase family

Homologues:

Organism=Homo sapiens, GI62912457, Length=286, Percent_Identity=32.8671328671329, Blast_Score=128, Evalue=4e-30,
Organism=Homo sapiens, GI21361368, Length=288, Percent_Identity=32.6388888888889, Blast_Score=126, Evalue=2e-29,
Organism=Escherichia coli, GI1786437, Length=256, Percent_Identity=38.28125, Blast_Score=174, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI17569967, Length=270, Percent_Identity=31.4814814814815, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17569969, Length=270, Percent_Identity=31.4814814814815, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321822, Length=258, Percent_Identity=39.1472868217054, Blast_Score=162, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6320506, Length=251, Percent_Identity=41.8326693227092, Blast_Score=160, Evalue=2e-40,
Organism=Drosophila melanogaster, GI21357643, Length=284, Percent_Identity=34.1549295774648, Blast_Score=139, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_LACPL (Q890J5)

Other databases:

- EMBL:   AL935252
- RefSeq:   NP_783880.1
- ProteinModelPortal:   Q890J5
- SMR:   Q890J5
- GeneID:   1061284
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0016
- NMPDR:   fig|220668.1.peg.14
- HOGENOM:   HBG507643
- OMA:   LINTIDP
- ProtClustDB:   PRK12314
- BioCyc:   LPLA220668:LP_0016-MONOMER
- BRENDA:   2.7.2.11
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; SSF53633 Aa_kinase

EC number: =2.7.2.11

Molecular weight: Translated: 29066; Mature: 28935

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVVKVIRKLKAKRVVIKVGTSTLVYPDGSINLRAIDQLAFVISAMRHRGREVVLVSSGA
CHHHHHHHHHHHCEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCC
IGVGLNYMGLKQRPKAIPEQQAIAAIGQTELISIYKERFAIYEQKIGQLLLTRDIFVYPK
EEECCCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEC
SHHNVLNTFEALLKQNVVPIVNENDTVAVDELDHETKFGDNDQLSAIVATNIGADLLIML
CCCHHHHHHHHHHHCCCCEEECCCCEEEEECCCCCCCCCCCCCEEEEEEECCCCEEEEEE
SDIDGFYDGNPNADARAQLLGHIEKVDQHTYKLAGGSGSRFGTGGMVTKLKAAERMIDAN
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHHCCC
GQMILANGADPTIIFQILAGEPVGTLFG
CCEEEECCCCCEEEEEEECCCCCHHCCC
>Mature Secondary Structure 
AVVKVIRKLKAKRVVIKVGTSTLVYPDGSINLRAIDQLAFVISAMRHRGREVVLVSSGA
HHHHHHHHHHHCEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCC
IGVGLNYMGLKQRPKAIPEQQAIAAIGQTELISIYKERFAIYEQKIGQLLLTRDIFVYPK
EEECCCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEC
SHHNVLNTFEALLKQNVVPIVNENDTVAVDELDHETKFGDNDQLSAIVATNIGADLLIML
CCCHHHHHHHHHHHCCCCEEECCCCEEEEECCCCCCCCCCCCCEEEEEEECCCCEEEEEE
SDIDGFYDGNPNADARAQLLGHIEKVDQHTYKLAGGSGSRFGTGGMVTKLKAAERMIDAN
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHHCCC
GQMILANGADPTIIFQILAGEPVGTLFG
CCEEEECCCCCEEEEEEECCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566