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The map label for this gene is recF

Identifier: 28376978

GI number: 28376978

Start: 3444

End: 4568

Strand: Direct

Name: recF

Synonym: lp_0005

Alternate gene names: 28376978

Gene position: 3444-4568 (Clockwise)

Preceding gene: 28376977

Following gene: 28376979

Centisome position: 0.1

GC content: 45.24

Gene sequence:

>1125_bases
ATGTACTTAGAAAACTTAGTCTTGCATGATTTTCGGAACTACGCGGATTTGACCATTAATTTTAGTCAGGGCGTTAATGT
TCTATTAGGTGAAAATGCGCAGGGAAAGACGAACCTGTTGGAAGCCATTTATGTGTTGGCGTTAACGCGTAGTCACCGCA
CCGCTAACGATAAGGAATTAATTCGGTGGCAAACCACCACGGCAACGTTGCAAGGCCGGTTACATAAAAGTACCGGTGCT
GTCCCCTTGGAACTTGAATTGGGGCGGCGGGGCAAGCGAGCGAAAGTCAATCATCTTGAGCAAGCCAAATTATCCCAGTA
CGTCGGAAATTTGAACGTGATCGTGTTTGCACCTGAAGATTTATCCATCGTTAAAGGGGCACCCGCGGTTCGCCGTCGGT
TTATGGATATGGAATTTGGTCAGATGAGCCCTAAATACCTCTATAACCTAAGTCAGTATCGCACGATTTTAAAACAACGT
AATCAATACTTACGGCAATTAAATCGGCAGCAGGCCAAGGATAAGGTTTATTTGGGTGTCTTGTCGGATCAATTGGCTGC
ATTTGGTGCGGAAATCATCCATAAACGACTACAACTGTTGCAGCAACTTGAGAAATGGGCGCAAGCTGTTCACAGCGAGA
TTACGCAAGAACAGGAACAATTAACGTTTCACTATGTCACTCAAGTGCCAACTGCCGACCAGACGTCGGTCGATCACATT
TATCAAACGCTACAGGCCTTATATCAGCAACAACAAGCCAAAGAAATTTTTCAAGGGACGACTTTATTAGGTCCTCATCG
CGATGATTTACAATTTGGTGTTAACGGCAAAAATGTGCAGACTTTTGGCTCCCAAGGCCAACAGCGTACAACTGCTTTGT
CCGTTAAGCTTGCTGAAATTGATCTGATGAAAGCTGAGACGGGCGAGTACCCGGTCTTATTATTAGACGATGTCTTATCA
GAATTAGACGCTGCACGGCAGACTCATCTGCTGACAGCGATCCAAGATAAAGTGCAGACCTTTTTAACCACGCCAAGTCT
TGACGGGGTGGCCCGCAAATTAATTAATGCGCCAAAAGTATTTGAAGTTAGCCATGGCACTTTACATGAGGAGGAACCGC
ATTGA

Upstream 100 bases:

>100_bases
AGATGATCGGCGGGGTCGGAAACTTTATCCAGGCGATACGATTGAAGTTGAAGACAACGGGTCATTTTTTATTCGCTCAA
ATCAAGAAACGACTGATTAG

Downstream 100 bases:

>100_bases
CCGAAAACGAAAAAGAAACCAAACTTGAACAGGCTCGCGAATATGATGCTAGTCAAATTCAAGTTTTGGAAGGTTTAGAA
GCTGTCCGGAAACGTCCCGG

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MYLENLVLHDFRNYADLTINFSQGVNVLLGENAQGKTNLLEAIYVLALTRSHRTANDKELIRWQTTTATLQGRLHKSTGA
VPLELELGRRGKRAKVNHLEQAKLSQYVGNLNVIVFAPEDLSIVKGAPAVRRRFMDMEFGQMSPKYLYNLSQYRTILKQR
NQYLRQLNRQQAKDKVYLGVLSDQLAAFGAEIIHKRLQLLQQLEKWAQAVHSEITQEQEQLTFHYVTQVPTADQTSVDHI
YQTLQALYQQQQAKEIFQGTTLLGPHRDDLQFGVNGKNVQTFGSQGQQRTTALSVKLAEIDLMKAETGEYPVLLLDDVLS
ELDAARQTHLLTAIQDKVQTFLTTPSLDGVARKLINAPKVFEVSHGTLHEEEPH

Sequences:

>Translated_374_residues
MYLENLVLHDFRNYADLTINFSQGVNVLLGENAQGKTNLLEAIYVLALTRSHRTANDKELIRWQTTTATLQGRLHKSTGA
VPLELELGRRGKRAKVNHLEQAKLSQYVGNLNVIVFAPEDLSIVKGAPAVRRRFMDMEFGQMSPKYLYNLSQYRTILKQR
NQYLRQLNRQQAKDKVYLGVLSDQLAAFGAEIIHKRLQLLQQLEKWAQAVHSEITQEQEQLTFHYVTQVPTADQTSVDHI
YQTLQALYQQQQAKEIFQGTTLLGPHRDDLQFGVNGKNVQTFGSQGQQRTTALSVKLAEIDLMKAETGEYPVLLLDDVLS
ELDAARQTHLLTAIQDKVQTFLTTPSLDGVARKLINAPKVFEVSHGTLHEEEPH
>Mature_374_residues
MYLENLVLHDFRNYADLTINFSQGVNVLLGENAQGKTNLLEAIYVLALTRSHRTANDKELIRWQTTTATLQGRLHKSTGA
VPLELELGRRGKRAKVNHLEQAKLSQYVGNLNVIVFAPEDLSIVKGAPAVRRRFMDMEFGQMSPKYLYNLSQYRTILKQR
NQYLRQLNRQQAKDKVYLGVLSDQLAAFGAEIIHKRLQLLQQLEKWAQAVHSEITQEQEQLTFHYVTQVPTADQTSVDHI
YQTLQALYQQQQAKEIFQGTTLLGPHRDDLQFGVNGKNVQTFGSQGQQRTTALSVKLAEIDLMKAETGEYPVLLLDDVLS
ELDAARQTHLLTAIQDKVQTFLTTPSLDGVARKLINAPKVFEVSHGTLHEEEPH

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=371, Percent_Identity=25.8760107816712, Blast_Score=121, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_LACPL (Q890K5)

Other databases:

- EMBL:   AL935252
- RefSeq:   NP_783870.1
- ProteinModelPortal:   Q890K5
- SMR:   Q890K5
- GeneID:   1061119
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0005
- NMPDR:   fig|220668.1.peg.4
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   PRK00064
- BioCyc:   LPLA220668:LP_0005-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 42439; Mature: 42439

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLENLVLHDFRNYADLTINFSQGVNVLLGENAQGKTNLLEAIYVLALTRSHRTANDKEL
CCHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCHHH
IRWQTTTATLQGRLHKSTGAVPLELELGRRGKRAKVNHLEQAKLSQYVGNLNVIVFAPED
EEEEEHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCC
LSIVKGAPAVRRRFMDMEFGQMSPKYLYNLSQYRTILKQRNQYLRQLNRQQAKDKVYLGV
CHHHCCCHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEH
LSDQLAAFGAEIIHKRLQLLQQLEKWAQAVHSEITQEQEQLTFHYVTQVPTADQTSVDHI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHH
YQTLQALYQQQQAKEIFQGTTLLGPHRDDLQFGVNGKNVQTFGSQGQQRTTALSVKLAEI
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCHHHCCCCCCHHHHHEEEEHHH
DLMKAETGEYPVLLLDDVLSELDAARQTHLLTAIQDKVQTFLTTPSLDGVARKLINAPKV
EEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCE
FEVSHGTLHEEEPH
EEECCCCCCCCCCC
>Mature Secondary Structure
MYLENLVLHDFRNYADLTINFSQGVNVLLGENAQGKTNLLEAIYVLALTRSHRTANDKEL
CCHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCHHH
IRWQTTTATLQGRLHKSTGAVPLELELGRRGKRAKVNHLEQAKLSQYVGNLNVIVFAPED
EEEEEHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCC
LSIVKGAPAVRRRFMDMEFGQMSPKYLYNLSQYRTILKQRNQYLRQLNRQQAKDKVYLGV
CHHHCCCHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEH
LSDQLAAFGAEIIHKRLQLLQQLEKWAQAVHSEITQEQEQLTFHYVTQVPTADQTSVDHI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHH
YQTLQALYQQQQAKEIFQGTTLLGPHRDDLQFGVNGKNVQTFGSQGQQRTTALSVKLAEI
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCHHHCCCCCCHHHHHEEEEHHH
DLMKAETGEYPVLLLDDVLSELDAARQTHLLTAIQDKVQTFLTTPSLDGVARKLINAPKV
EEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCE
FEVSHGTLHEEEPH
EEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566