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The map label for this gene is gyrB [H]

Identifier: 28376979

GI number: 28376979

Start: 4565

End: 6511

Strand: Direct

Name: gyrB [H]

Synonym: lp_0006

Alternate gene names: 28376979

Gene position: 4565-6511 (Clockwise)

Preceding gene: 28376978

Following gene: 28376980

Centisome position: 0.14

GC content: 45.92

Gene sequence:

>1947_bases
TTGACCGAAAACGAAAAAGAAACCAAACTTGAACAGGCTCGCGAATATGATGCTAGTCAAATTCAAGTTTTGGAAGGTTT
AGAAGCTGTCCGGAAACGTCCCGGAATGTATATCGGTACGACGAGTAGCCAAGGACTCCACCACTTAGTTTGGGAAATTG
TTGATAACGGAATTGATGAAGCCCTAGCAGGGTTTGCAAACGTGATCCACGTGACCGTTGAAAAGGATAATAGTATTACG
GTTACTGATAACGGTCGTGGGATTCCTGTTGATATTCAAGAAAAAACGGGTAAACCAGCGCTTGAAACGGTTTATACGAT
TTTACATGCCGGTGGTAAATTCGGTGGTGGCGGTTATAAAGTCTCTGGTGGTCTTCACGGGGTCGGGGCTTCCGTTGTTA
ACGCCTTATCATCGACCCTGGACGTCAAAGTAACTCGGAATGGTCATGTTTATTACATGGACTTTGAACGTGGAAAGGTT
AAAACACCGATGAAGATCATTGGTGATGCGCCTGATGATGCTCATGGTACGGCGGTTCATTTTGTTCCGGATCCTGATAT
TTTCCGGGAAACGACCACCTATGACATTAATATTTTGACGACTCGGATTCGCGAACTCGCTTTCTTAAATAAGGGATTAC
GGATCACGATTCGGGATAATCGCCCTGATGAACCCACTGAAGATGACTTTCTCTACGAAGGTGGGATTCGTCACTATGTT
GAATATCTAGATAAGAATAAGACCGTTCTATTTCCAGAACCGATTTATGTGGAAGGGGAACAGAATGGGATTACGGTAGA
AGTTGCGTTGCAATATACTGACGACTACCACAGTAATTTGATGACCTTCACCAACAATATTCATACGTACGAAGGTGGGA
CGCATGAAGAAGGTTTCAAACGTGCTTTGACGCGGGTCATCAACGACTATGCTCGTAAGAATAACTTGTTGAAGGAAAGC
GATGCGAACCTATCTGGCGAAGATGTTCGTGAAGGGATGACGGCTGTTGTCAGCGTCAAGCATCCGGATCCTCAATTTGA
AGGTCAAACGAAGACGAAACTTGGTAATTCCGATGCTCGGGCAGCAGTTGACCGGTTATTCTCAGAACACTTTAGCAAGT
ACTTGATGGAAAACCCAAGTGTTGCCCGTAAAGTGGTCGACAAAGGGTTACTTGCATCCAAGGCCCGGGTCGCTGCTAAG
CGTGCGCGGGAAGTTACGCGAAAAAAGAGTGGCTTGGAAATTAGTAATTTACCAGGTAAGTTGGCGGACAACAGTAGTAA
AGACCCTGAAATTAGTGAGTTGTTCATTGTCGAAGGGGATTCTGCCGGTGGGTCGGCTAAGCAGGGACGTTCCCGGTTGA
CGCAAGCGATTTTACCGATTCGGGGTAAAATTTTGAACGTTGAAAAGGCCAGCATTGATAAGATTTTAGCCAATGAAGAA
ATCCGGTCATTGTTTACGGCAATGGGAACTGGCTTCGGTGGTGACTTCGACTTGAGCAAAGCCAATTATCACAAATTAAT
TATCATGACTGATGCCGATGTCGATGGGGCACATATTCGGACCTTGTTATTGACGTTGTTTTACCGGTACATGCGGCCAT
TAGTTGACGCTGGCTTTGTGTACATCGCTCAACCACCGTTGTACCAAGTCCGGCAAGGTAAATTTGTGAAGTATATTGAT
TCAGATGAAGAATTGAGTGAGGTCATGGGGCAATTGGCACCATCACCGAAACCAGTTGTTCAACGGTACAAGGGGCTTGG
TGAAATGGACGCGGAACAATTATGGGAAACGACCATGGATCCAGACAAGCGGCGCCTATTACGGGTTCGTGATGAAGACG
CTGCGGATGCCGATGGGGTCTTCTCGATGTTGATGGGGGACCAAGTCAAACCACGGCGAGAATTTATTGAAGATAATGCC
AAGTTCGTCCAGGATCTGGATGTTTAG

Upstream 100 bases:

>100_bases
TTTTAACCACGCCAAGTCTTGACGGGGTGGCCCGCAAATTAATTAATGCGCCAAAAGTATTTGAAGTTAGCCATGGCACT
TTACATGAGGAGGAACCGCA

Downstream 100 bases:

>100_bases
TGTGTCAGTTTAGTCATTTTGACCTAAGAACTGAGGCGCAAGCTGTTAATCAGACTTAGGGTCGTAATGGCACGACGGTT
GTCTTAATGGCGACTTTTAC

Product: DNA gyrase, B subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 648; Mature: 647

Protein sequence:

>648_residues
MTENEKETKLEQAREYDASQIQVLEGLEAVRKRPGMYIGTTSSQGLHHLVWEIVDNGIDEALAGFANVIHVTVEKDNSIT
VTDNGRGIPVDIQEKTGKPALETVYTILHAGGKFGGGGYKVSGGLHGVGASVVNALSSTLDVKVTRNGHVYYMDFERGKV
KTPMKIIGDAPDDAHGTAVHFVPDPDIFRETTTYDINILTTRIRELAFLNKGLRITIRDNRPDEPTEDDFLYEGGIRHYV
EYLDKNKTVLFPEPIYVEGEQNGITVEVALQYTDDYHSNLMTFTNNIHTYEGGTHEEGFKRALTRVINDYARKNNLLKES
DANLSGEDVREGMTAVVSVKHPDPQFEGQTKTKLGNSDARAAVDRLFSEHFSKYLMENPSVARKVVDKGLLASKARVAAK
RAREVTRKKSGLEISNLPGKLADNSSKDPEISELFIVEGDSAGGSAKQGRSRLTQAILPIRGKILNVEKASIDKILANEE
IRSLFTAMGTGFGGDFDLSKANYHKLIIMTDADVDGAHIRTLLLTLFYRYMRPLVDAGFVYIAQPPLYQVRQGKFVKYID
SDEELSEVMGQLAPSPKPVVQRYKGLGEMDAEQLWETTMDPDKRRLLRVRDEDAADADGVFSMLMGDQVKPRREFIEDNA
KFVQDLDV

Sequences:

>Translated_648_residues
MTENEKETKLEQAREYDASQIQVLEGLEAVRKRPGMYIGTTSSQGLHHLVWEIVDNGIDEALAGFANVIHVTVEKDNSIT
VTDNGRGIPVDIQEKTGKPALETVYTILHAGGKFGGGGYKVSGGLHGVGASVVNALSSTLDVKVTRNGHVYYMDFERGKV
KTPMKIIGDAPDDAHGTAVHFVPDPDIFRETTTYDINILTTRIRELAFLNKGLRITIRDNRPDEPTEDDFLYEGGIRHYV
EYLDKNKTVLFPEPIYVEGEQNGITVEVALQYTDDYHSNLMTFTNNIHTYEGGTHEEGFKRALTRVINDYARKNNLLKES
DANLSGEDVREGMTAVVSVKHPDPQFEGQTKTKLGNSDARAAVDRLFSEHFSKYLMENPSVARKVVDKGLLASKARVAAK
RAREVTRKKSGLEISNLPGKLADNSSKDPEISELFIVEGDSAGGSAKQGRSRLTQAILPIRGKILNVEKASIDKILANEE
IRSLFTAMGTGFGGDFDLSKANYHKLIIMTDADVDGAHIRTLLLTLFYRYMRPLVDAGFVYIAQPPLYQVRQGKFVKYID
SDEELSEVMGQLAPSPKPVVQRYKGLGEMDAEQLWETTMDPDKRRLLRVRDEDAADADGVFSMLMGDQVKPRREFIEDNA
KFVQDLDV
>Mature_647_residues
TENEKETKLEQAREYDASQIQVLEGLEAVRKRPGMYIGTTSSQGLHHLVWEIVDNGIDEALAGFANVIHVTVEKDNSITV
TDNGRGIPVDIQEKTGKPALETVYTILHAGGKFGGGGYKVSGGLHGVGASVVNALSSTLDVKVTRNGHVYYMDFERGKVK
TPMKIIGDAPDDAHGTAVHFVPDPDIFRETTTYDINILTTRIRELAFLNKGLRITIRDNRPDEPTEDDFLYEGGIRHYVE
YLDKNKTVLFPEPIYVEGEQNGITVEVALQYTDDYHSNLMTFTNNIHTYEGGTHEEGFKRALTRVINDYARKNNLLKESD
ANLSGEDVREGMTAVVSVKHPDPQFEGQTKTKLGNSDARAAVDRLFSEHFSKYLMENPSVARKVVDKGLLASKARVAAKR
AREVTRKKSGLEISNLPGKLADNSSKDPEISELFIVEGDSAGGSAKQGRSRLTQAILPIRGKILNVEKASIDKILANEEI
RSLFTAMGTGFGGDFDLSKANYHKLIIMTDADVDGAHIRTLLLTLFYRYMRPLVDAGFVYIAQPPLYQVRQGKFVKYIDS
DEELSEVMGQLAPSPKPVVQRYKGLGEMDAEQLWETTMDPDKRRLLRVRDEDAADADGVFSMLMGDQVKPRREFIEDNAK
FVQDLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=651, Percent_Identity=25.0384024577573, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI19913406, Length=666, Percent_Identity=24.6246246246246, Blast_Score=127, Evalue=3e-29,
Organism=Escherichia coli, GI48994957, Length=560, Percent_Identity=52.8571428571429, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI1789408, Length=636, Percent_Identity=40.5660377358491, Blast_Score=432, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17535065, Length=615, Percent_Identity=25.0406504065041, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI212645845, Length=583, Percent_Identity=23.6706689536878, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI212645657, Length=230, Percent_Identity=25.6521739130435, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=598, Percent_Identity=24.7491638795987, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI17136538, Length=664, Percent_Identity=24.2469879518072, Blast_Score=130, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 72126; Mature: 71995

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENEKETKLEQAREYDASQIQVLEGLEAVRKRPGMYIGTTSSQGLHHLVWEIVDNGIDE
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCHHH
ALAGFANVIHVTVEKDNSITVTDNGRGIPVDIQEKTGKPALETVYTILHAGGKFGGGGYK
HHHHHHEEEEEEEECCCEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEE
VSGGLHGVGASVVNALSSTLDVKVTRNGHVYYMDFERGKVKTPMKIIGDAPDDAHGTAVH
ECCCCCHHHHHHHHHHHHCEEEEEECCCEEEEEECCCCCCCCCHHHCCCCCCCCCCCEEE
FVPDPDIFRETTTYDINILTTRIRELAFLNKGLRITIRDNRPDEPTEDDFLYEGGIRHYV
ECCCCHHHHCCCEEEEHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEECCCHHHHH
EYLDKNKTVLFPEPIYVEGEQNGITVEVALQYTDDYHSNLMTFTNNIHTYEGGTHEEGFK
HHHCCCCEEECCCCEEEECCCCCEEEEEEEEECCCHHCCCEEEECCEEECCCCCCHHHHH
RALTRVINDYARKNNLLKESDANLSGEDVREGMTAVVSVKHPDPQFEGQTKTKLGNSDAR
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCHHHCCCCCHHH
AAVDRLFSEHFSKYLMENPSVARKVVDKGLLASKARVAAKRAREVTRKKSGLEISNLPGK
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCC
LADNSSKDPEISELFIVEGDSAGGSAKQGRSRLTQAILPIRGKILNVEKASIDKILANEE
CCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHH
IRSLFTAMGTGFGGDFDLSKANYHKLIIMTDADVDGAHIRTLLLTLFYRYMRPLVDAGFV
HHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEE
YIAQPPLYQVRQGKFVKYIDSDEELSEVMGQLAPSPKPVVQRYKGLGEMDAEQLWETTMD
EEECCCHHHHCCCCEEEECCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHCCC
PDKRRLLRVRDEDAADADGVFSMLMGDQVKPRREFIEDNAKFVQDLDV
CCHHHHEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TENEKETKLEQAREYDASQIQVLEGLEAVRKRPGMYIGTTSSQGLHHLVWEIVDNGIDE
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCHHH
ALAGFANVIHVTVEKDNSITVTDNGRGIPVDIQEKTGKPALETVYTILHAGGKFGGGGYK
HHHHHHEEEEEEEECCCEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEE
VSGGLHGVGASVVNALSSTLDVKVTRNGHVYYMDFERGKVKTPMKIIGDAPDDAHGTAVH
ECCCCCHHHHHHHHHHHHCEEEEEECCCEEEEEECCCCCCCCCHHHCCCCCCCCCCCEEE
FVPDPDIFRETTTYDINILTTRIRELAFLNKGLRITIRDNRPDEPTEDDFLYEGGIRHYV
ECCCCHHHHCCCEEEEHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEECCCHHHHH
EYLDKNKTVLFPEPIYVEGEQNGITVEVALQYTDDYHSNLMTFTNNIHTYEGGTHEEGFK
HHHCCCCEEECCCCEEEECCCCCEEEEEEEEECCCHHCCCEEEECCEEECCCCCCHHHHH
RALTRVINDYARKNNLLKESDANLSGEDVREGMTAVVSVKHPDPQFEGQTKTKLGNSDAR
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCHHHCCCCCHHH
AAVDRLFSEHFSKYLMENPSVARKVVDKGLLASKARVAAKRAREVTRKKSGLEISNLPGK
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCC
LADNSSKDPEISELFIVEGDSAGGSAKQGRSRLTQAILPIRGKILNVEKASIDKILANEE
CCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHH
IRSLFTAMGTGFGGDFDLSKANYHKLIIMTDADVDGAHIRTLLLTLFYRYMRPLVDAGFV
HHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEE
YIAQPPLYQVRQGKFVKYIDSDEELSEVMGQLAPSPKPVVQRYKGLGEMDAEQLWETTMD
EEECCCHHHHCCCCEEEECCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHCCC
PDKRRLLRVRDEDAADADGVFSMLMGDQVKPRREFIEDNAKFVQDLDV
CCHHHHEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377 [H]