Click here to switch to the map view.

The map label for this gene is pilS [H]

Identifier: 28199796

GI number: 28199796

Start: 2263218

End: 2264828

Strand: Reverse

Name: pilS [H]

Synonym: PD1929

Alternate gene names: 28199796

Gene position: 2264828-2263218 (Counterclockwise)

Preceding gene: 28199800

Following gene: 28199795

Centisome position: 89.88

GC content: 51.15

Gene sequence:

>1611_bases
GTGTTGTTCAGCGCTTCGCTGATTCATCGGATCGAATTTGCCCCGCAGCGTGAACTTTATTTTCTCGCGTTGTATCGGAT
CTTGGAGGCTGGATTGATCGCGGCCCTGGTGTTCAGTCCGGTGTCGCTATTGGTGGATCCTGAGAGTGATCCACATTTGG
GCACGGTTGTCGCTGTCGGTTATTTGCTGTTGGCTACCATTTTGTTGGTCTGGGGTCGTAATGAGCGTTGGTTCACGCCA
TTAGTCTTTGGAAGTACTGTCGCCGACATTTTGGCTGCCACTCTTGCTATTCACGCTCTGCCTAAAGCCAGTGCCGGCAT
TGCGATGATGTTGTTGTTTAACGTTGCTGCCGCCGCCATCTTATTGCGGTTGCGTTTCAGCATGATCCTGGCTTGGATTG
CTGGGGTTGCGATGCTTGTGGAATACATCTGGACTGTACTCAGTGAGGGTGACTCCTCACGTTCACTGGCCGAGCTCCTT
ATGTTTTTCACCAGCTACACTGCAGTGGCGTATATGTGTCATCAGGCCGCGCAGCGGGCTAGATATAGCCAAGCTCTGGC
TGAAAAGCGCCGTGCCCAGATAGTCAACTTGGCAGAGATCAATGAGCTGATTATCCGACGTATGCGGACGGGTGTGTTGG
TGGTGGATGCTGCTAATCGAATCGGTTTGAGTAACGAAACTGCAGCTGCGTTGCTGGGATACGACAGACAATTGGTCGAG
AGTGATTACGATCTCGCTACGGTTGCTCCGGAGTTGATAATGCGTCTGAAGTGTTGGCGTATTGGTCGACACTACGAAGA
TACTCCGTTGCGATTAGCGCCTGATCAACCTGAGGTTCAGCCTCGCTTTGTGAGTTTGCTTGCCGACAGTGGATTGACAT
TAATTTTCCTTGATGATGTCAGTGTTGTTTCACGCCGTGCCGAATCGTTGACTTTGTTGGCGCTTGGACGTTTTTCAGCC
AGTTTGGCGCACGAGATTCGTAATCCTTTGGCTGCAATTAAACATGCATCGCAGCTGTTGGAAGAGTCCTCCAATCTTGA
CAAAGGCGATCAAAGGCTGTTGGACATTATTCTAAAGCAGTGCCAGCGGACCAATGGGATTGTGGAGAGCGTGCTTGGTT
TGGCAAGACGCGAACGTGCTAACCCGGAAAATTTGGATTTGGTCGGTTTTGTGTCGCGATTTGTCGATGAATACCGCCAG
ACATTGTCAAGCGAGTATGACAATCTGGAGATACAAACGTTGCACAGTACCGTGCAGGCACTGGTGGATCCACGCCACTT
GTACCAGATCCTCGCTGTCCTTACCCACAACGCATTGCGTTATGGCCGCTTTCCGGATACTCCGGCACAGGTGCGTATTG
AGGTGGTATATACCGGAGGCAGCGTGGGGATCAATGTCCTTGATCAGGGGCCTGGTATACCCGATGCGGCTGTTTTGCAG
CTTTTTCAGCCTTTTTTCACTACTTCCGACCATGGTACGGGGCTTGGTCTATATATCGCTCGTGAGCTGTGCAATGCGAA
TCAATCCAATCTTGAGTACGTGGCCATTCCAGGGGGAGGGGCTTGTTTCCGGATTACTTTGCCGGGTCCGTATGCATTGA
TTCCGAATTGA

Upstream 100 bases:

>100_bases
TTCTTGTAATTTTTGCGGGGCTAGTTGGAGTTAATCGGGCATAGTGTCGCCGGTCGTGTCTATACTCGGCTATCTCTCTT
CTGCCTTAGGACACCGTGAC

Downstream 100 bases:

>100_bases
GTGCCCCGGTATTTCATTCACATTCTTTCGCATTTAGAGAGAGCGTGGCCGTTGCGGCGTGTTAGCGTCGCTATTGCAGA
TTGCAACCCTATGGGCGGTT

Product: two-component system, sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MLFSASLIHRIEFAPQRELYFLALYRILEAGLIAALVFSPVSLLVDPESDPHLGTVVAVGYLLLATILLVWGRNERWFTP
LVFGSTVADILAATLAIHALPKASAGIAMMLLFNVAAAAILLRLRFSMILAWIAGVAMLVEYIWTVLSEGDSSRSLAELL
MFFTSYTAVAYMCHQAAQRARYSQALAEKRRAQIVNLAEINELIIRRMRTGVLVVDAANRIGLSNETAAALLGYDRQLVE
SDYDLATVAPELIMRLKCWRIGRHYEDTPLRLAPDQPEVQPRFVSLLADSGLTLIFLDDVSVVSRRAESLTLLALGRFSA
SLAHEIRNPLAAIKHASQLLEESSNLDKGDQRLLDIILKQCQRTNGIVESVLGLARRERANPENLDLVGFVSRFVDEYRQ
TLSSEYDNLEIQTLHSTVQALVDPRHLYQILAVLTHNALRYGRFPDTPAQVRIEVVYTGGSVGINVLDQGPGIPDAAVLQ
LFQPFFTTSDHGTGLGLYIARELCNANQSNLEYVAIPGGGACFRITLPGPYALIPN

Sequences:

>Translated_536_residues
MLFSASLIHRIEFAPQRELYFLALYRILEAGLIAALVFSPVSLLVDPESDPHLGTVVAVGYLLLATILLVWGRNERWFTP
LVFGSTVADILAATLAIHALPKASAGIAMMLLFNVAAAAILLRLRFSMILAWIAGVAMLVEYIWTVLSEGDSSRSLAELL
MFFTSYTAVAYMCHQAAQRARYSQALAEKRRAQIVNLAEINELIIRRMRTGVLVVDAANRIGLSNETAAALLGYDRQLVE
SDYDLATVAPELIMRLKCWRIGRHYEDTPLRLAPDQPEVQPRFVSLLADSGLTLIFLDDVSVVSRRAESLTLLALGRFSA
SLAHEIRNPLAAIKHASQLLEESSNLDKGDQRLLDIILKQCQRTNGIVESVLGLARRERANPENLDLVGFVSRFVDEYRQ
TLSSEYDNLEIQTLHSTVQALVDPRHLYQILAVLTHNALRYGRFPDTPAQVRIEVVYTGGSVGINVLDQGPGIPDAAVLQ
LFQPFFTTSDHGTGLGLYIARELCNANQSNLEYVAIPGGGACFRITLPGPYALIPN
>Mature_536_residues
MLFSASLIHRIEFAPQRELYFLALYRILEAGLIAALVFSPVSLLVDPESDPHLGTVVAVGYLLLATILLVWGRNERWFTP
LVFGSTVADILAATLAIHALPKASAGIAMMLLFNVAAAAILLRLRFSMILAWIAGVAMLVEYIWTVLSEGDSSRSLAELL
MFFTSYTAVAYMCHQAAQRARYSQALAEKRRAQIVNLAEINELIIRRMRTGVLVVDAANRIGLSNETAAALLGYDRQLVE
SDYDLATVAPELIMRLKCWRIGRHYEDTPLRLAPDQPEVQPRFVSLLADSGLTLIFLDDVSVVSRRAESLTLLALGRFSA
SLAHEIRNPLAAIKHASQLLEESSNLDKGDQRLLDIILKQCQRTNGIVESVLGLARRERANPENLDLVGFVSRFVDEYRQ
TLSSEYDNLEIQTLHSTVQALVDPRHLYQILAVLTHNALRYGRFPDTPAQVRIEVVYTGGSVGINVLDQGPGIPDAAVLQ
LFQPFFTTSDHGTGLGLYIARELCNANQSNLEYVAIPGGGACFRITLPGPYALIPN

Specific function: Member of the two-component regulatory system pilS/pilR involved in the regulation of the expression of type 4 fimbriae. PilS may function as a membrane-associated protein kinase that phosphorylates pilR in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=368, Percent_Identity=26.3586956521739, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1790436, Length=229, Percent_Identity=29.2576419213974, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI87082128, Length=235, Percent_Identity=26.8085106382979, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788713, Length=234, Percent_Identity=28.2051282051282, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI145693157, Length=282, Percent_Identity=27.3049645390071, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1786600, Length=223, Percent_Identity=25.5605381165919, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI1789808, Length=235, Percent_Identity=26.8085106382979, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790300, Length=225, Percent_Identity=29.7777777777778, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786912, Length=218, Percent_Identity=26.605504587156, Blast_Score=63, Evalue=5e-11,
Organism=Escherichia coli, GI1790861, Length=192, Percent_Identity=29.6875, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 59136; Mature: 59136

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFSASLIHRIEFAPQRELYFLALYRILEAGLIAALVFSPVSLLVDPESDPHLGTVVAVG
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCCCCHHHHHHHH
YLLLATILLVWGRNERWFTPLVFGSTVADILAATLAIHALPKASAGIAMMLLFNVAAAAI
HHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
LLRLRFSMILAWIAGVAMLVEYIWTVLSEGDSSRSLAELLMFFTSYTAVAYMCHQAAQRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYSQALAEKRRAQIVNLAEINELIIRRMRTGVLVVDAANRIGLSNETAAALLGYDRQLVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHCCHHHHH
SDYDLATVAPELIMRLKCWRIGRHYEDTPLRLAPDQPEVQPRFVSLLADSGLTLIFLDDV
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCHHHHHHHHCCCCEEEEECCH
SVVSRRAESLTLLALGRFSASLAHEIRNPLAAIKHASQLLEESSNLDKGDQRLLDIILKQ
HHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
CQRTNGIVESVLGLARRERANPENLDLVGFVSRFVDEYRQTLSSEYDNLEIQTLHSTVQA
HHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LVDPRHLYQILAVLTHNALRYGRFPDTPAQVRIEVVYTGGSVGINVLDQGPGIPDAAVLQ
HHCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCCCHHHHHH
LFQPFFTTSDHGTGLGLYIARELCNANQSNLEYVAIPGGGACFRITLPGPYALIPN
HHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEECCCCEEECCC
>Mature Secondary Structure
MLFSASLIHRIEFAPQRELYFLALYRILEAGLIAALVFSPVSLLVDPESDPHLGTVVAVG
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCCCCHHHHHHHH
YLLLATILLVWGRNERWFTPLVFGSTVADILAATLAIHALPKASAGIAMMLLFNVAAAAI
HHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
LLRLRFSMILAWIAGVAMLVEYIWTVLSEGDSSRSLAELLMFFTSYTAVAYMCHQAAQRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYSQALAEKRRAQIVNLAEINELIIRRMRTGVLVVDAANRIGLSNETAAALLGYDRQLVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHCCHHHHH
SDYDLATVAPELIMRLKCWRIGRHYEDTPLRLAPDQPEVQPRFVSLLADSGLTLIFLDDV
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCHHHHHHHHCCCCEEEEECCH
SVVSRRAESLTLLALGRFSASLAHEIRNPLAAIKHASQLLEESSNLDKGDQRLLDIILKQ
HHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
CQRTNGIVESVLGLARRERANPENLDLVGFVSRFVDEYRQTLSSEYDNLEIQTLHSTVQA
HHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LVDPRHLYQILAVLTHNALRYGRFPDTPAQVRIEVVYTGGSVGINVLDQGPGIPDAAVLQ
HHCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCCCHHHHHH
LFQPFFTTSDHGTGLGLYIARELCNANQSNLEYVAIPGGGACFRITLPGPYALIPN
HHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEECCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8097014; 10984043 [H]