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The map label for this gene is pilR [H]

Identifier: 28199795

GI number: 28199795

Start: 2261620

End: 2263032

Strand: Reverse

Name: pilR [H]

Synonym: PD1928

Alternate gene names: 28199795

Gene position: 2263032-2261620 (Counterclockwise)

Preceding gene: 28199796

Following gene: 28199792

Centisome position: 89.81

GC content: 52.51

Gene sequence:

>1413_bases
ATGAATGAATCCCGCAGTGCTCTGATTGTCGATGACGAGCGCGACATCCGTGAACTTTTGGTCCTTACGCTAGGACGTAT
GGGGCTGCGTATCAGAACGGCAGCTAATCTGGCACAAGCATACGCGTTACTTGAGTCTGACTCGTTCAATCTTTGTATCA
CCGACATGCGTTTACCTGACGGCAGTGGCATTGAGCTGGTGAAAAAGATTGTGCGTCATTATCCACGCACCCCGGTGGCC
ATGATCACTGCTTTTGGCAGTATCGAACTGGCGGTAGAGGCGCTCAAGGAAGGTGCTTTTGACTTTGTCAGCAAACCTGT
TGATCTGAACGTCCTGCGAGGCATGGTCCGTCATGCTTTGGAATTGAACAATCGTGAACGCTCTGTTGTGTCAAGTCAGC
CACAAAGCCCCAGCCAACGTCTCCTTGGTGAATCATCACCGATGGCTCAGCTTCGGGCCACCATCGCTAAAGTGGCGCGT
AGTCAAGCCCCGGTTTATATCCTTGGTGAATCAGGAGTTGGTAAAGAACTGGTTGCACGTACGATCCACGAACAGAGTGT
GCGTGCTGCTGGGGCGTTCGTGCCGGTCAACTGTGGTGCGATCCCTGTGGATCTTATGGAAAGCGAATTTTTCGGTCACA
AGAAAGGGAGTTTCACCGGTGCGCATACTGATAAGCCAGGTTTATTCCAAAGTGCTGGCGGCGGTACTTTATTCCTGGAT
GAAGTTGCCGAATTACCCTTGCACATGCAAGTTAAATTGCTGCGTGCGATTCAGGAGAAATCGATACGGCCAATCGGTGC
AACCGTTGAGGTACCAGTAGATGTGCGTATTTTATCGGCGACTCACCAAGATCTGAGTGACCTGATTGCCAATGGCCGCT
TCCGTCACGATTTGTACTACCGCATTAATGTAATCGAGATACGTGTTCCACCGCTACGTGAGCGTGCTGGCGATTTGCCG
CATCTTGCTGCAGCCATTGTGGCTCGCTTGGCACACACGCATGGGCGGGCGGTCCCGTTGCTTTCACCCTCCGCCTTGGA
GGCGTTAGAACATTATTCGTTCCCGGGTAATGTGCGTGAACTGGAGAACATTCTGGAGCGTGCTCTAGCACTGACTGAAG
ATGATCATATCGGTGCCGCGGATCTGCACCTGCCCCAGGGCAGTTTGCATGGACAGGGATTGCCGCTCCCGTTAGTGCCC
ACAGTTCTCCCGATTGTGCCTGCGGACGCATCAGCATCGGTGGAGGGTGAGAATACGCCGGCTGCCTTGCCCTCGTACAT
GGAGCAGCTGGAACGTGCTGCCATTCAAAAGGCGTTGGAAGAGAATCGCTGGAATAAAACAAAGACAGCCGCAAAACTTG
GCATCACTTTCCGTGCATTACGTTACAAACTAAAGAAGCTGGGTATGGAGTGA

Upstream 100 bases:

>100_bases
ACCCTATGGGCGGTTAATATTGGCATCTTCGATGCGTTGCCGTGCGTCTGAAGACGATGGGAATTGTCCATCTTCAGTCT
GCTGCGTTATCTTGGCATTC

Downstream 100 bases:

>100_bases
TGAGAGGAAGCAGGCAACATAGCCTGCCTTGCTTCTTCTCAGCATGGTGTCATGACGTGCTTTGATTCAGTCATTAACAG
GTGTTGTTGATGCCTAATCT

Product: two-component system, regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MNESRSALIVDDERDIRELLVLTLGRMGLRIRTAANLAQAYALLESDSFNLCITDMRLPDGSGIELVKKIVRHYPRTPVA
MITAFGSIELAVEALKEGAFDFVSKPVDLNVLRGMVRHALELNNRERSVVSSQPQSPSQRLLGESSPMAQLRATIAKVAR
SQAPVYILGESGVGKELVARTIHEQSVRAAGAFVPVNCGAIPVDLMESEFFGHKKGSFTGAHTDKPGLFQSAGGGTLFLD
EVAELPLHMQVKLLRAIQEKSIRPIGATVEVPVDVRILSATHQDLSDLIANGRFRHDLYYRINVIEIRVPPLRERAGDLP
HLAAAIVARLAHTHGRAVPLLSPSALEALEHYSFPGNVRELENILERALALTEDDHIGAADLHLPQGSLHGQGLPLPLVP
TVLPIVPADASASVEGENTPAALPSYMEQLERAAIQKALEENRWNKTKTAAKLGITFRALRYKLKKLGME

Sequences:

>Translated_470_residues
MNESRSALIVDDERDIRELLVLTLGRMGLRIRTAANLAQAYALLESDSFNLCITDMRLPDGSGIELVKKIVRHYPRTPVA
MITAFGSIELAVEALKEGAFDFVSKPVDLNVLRGMVRHALELNNRERSVVSSQPQSPSQRLLGESSPMAQLRATIAKVAR
SQAPVYILGESGVGKELVARTIHEQSVRAAGAFVPVNCGAIPVDLMESEFFGHKKGSFTGAHTDKPGLFQSAGGGTLFLD
EVAELPLHMQVKLLRAIQEKSIRPIGATVEVPVDVRILSATHQDLSDLIANGRFRHDLYYRINVIEIRVPPLRERAGDLP
HLAAAIVARLAHTHGRAVPLLSPSALEALEHYSFPGNVRELENILERALALTEDDHIGAADLHLPQGSLHGQGLPLPLVP
TVLPIVPADASASVEGENTPAALPSYMEQLERAAIQKALEENRWNKTKTAAKLGITFRALRYKLKKLGME
>Mature_470_residues
MNESRSALIVDDERDIRELLVLTLGRMGLRIRTAANLAQAYALLESDSFNLCITDMRLPDGSGIELVKKIVRHYPRTPVA
MITAFGSIELAVEALKEGAFDFVSKPVDLNVLRGMVRHALELNNRERSVVSSQPQSPSQRLLGESSPMAQLRATIAKVAR
SQAPVYILGESGVGKELVARTIHEQSVRAAGAFVPVNCGAIPVDLMESEFFGHKKGSFTGAHTDKPGLFQSAGGGTLFLD
EVAELPLHMQVKLLRAIQEKSIRPIGATVEVPVDVRILSATHQDLSDLIANGRFRHDLYYRINVIEIRVPPLRERAGDLP
HLAAAIVARLAHTHGRAVPLLSPSALEALEHYSFPGNVRELENILERALALTEDDHIGAADLHLPQGSLHGQGLPLPLVP
TVLPIVPADASASVEGENTPAALPSYMEQLERAAIQKALEENRWNKTKTAAKLGITFRALRYKLKKLGME

Specific function: Member of the two-component regulatory system pilS/pilR that regulates the expression of type 4 fimbriae. When activated by pilS it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=468, Percent_Identity=39.957264957265, Blast_Score=328, Evalue=5e-91,
Organism=Escherichia coli, GI1790437, Length=463, Percent_Identity=39.524838012959, Blast_Score=307, Evalue=7e-85,
Organism=Escherichia coli, GI1790299, Length=471, Percent_Identity=36.3057324840764, Blast_Score=291, Evalue=5e-80,
Organism=Escherichia coli, GI1788905, Length=460, Percent_Identity=37.6086956521739, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI1789233, Length=339, Percent_Identity=40.4129793510324, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI87082117, Length=244, Percent_Identity=45.0819672131148, Blast_Score=225, Evalue=6e-60,
Organism=Escherichia coli, GI1789087, Length=333, Percent_Identity=38.4384384384384, Blast_Score=222, Evalue=5e-59,
Organism=Escherichia coli, GI87082152, Length=331, Percent_Identity=41.0876132930514, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1786524, Length=393, Percent_Identity=36.3867684478371, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI87081872, Length=330, Percent_Identity=35.1515151515151, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1787583, Length=360, Percent_Identity=33.3333333333333, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1789828, Length=217, Percent_Identity=41.4746543778802, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI87081858, Length=227, Percent_Identity=35.2422907488987, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1789809, Length=139, Percent_Identity=30.9352517985612, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51273; Mature: 51273

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNESRSALIVDDERDIRELLVLTLGRMGLRIRTAANLAQAYALLESDSFNLCITDMRLPD
CCCCCCEEEEECHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHCCCCCCEEEEEEECCC
GSGIELVKKIVRHYPRTPVAMITAFGSIELAVEALKEGAFDFVSKPVDLNVLRGMVRHAL
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHHH
ELNNRERSVVSSQPQSPSQRLLGESSPMAQLRATIAKVARSQAPVYILGESGVGKELVAR
HHCCCHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHH
TIHEQSVRAAGAFVPVNCGAIPVDLMESEFFGHKKGSFTGAHTDKPGLFQSAGGGTLFLD
HHHHHHHHHCCCEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCEEEHH
EVAELPLHMQVKLLRAIQEKSIRPIGATVEVPVDVRILSATHQDLSDLIANGRFRHDLYY
HHHCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCEEEEHHHHHHHHHHHHCCCEEEEEEE
RINVIEIRVPPLRERAGDLPHLAAAIVARLAHTHGRAVPLLSPSALEALEHYSFPGNVRE
EEEEEEEECCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCHHH
LENILERALALTEDDHIGAADLHLPQGSLHGQGLPLPLVPTVLPIVPADASASVEGENTP
HHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCC
AALPSYMEQLERAAIQKALEENRWNKTKTAAKLGITFRALRYKLKKLGME
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNESRSALIVDDERDIRELLVLTLGRMGLRIRTAANLAQAYALLESDSFNLCITDMRLPD
CCCCCCEEEEECHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHCCCCCCEEEEEEECCC
GSGIELVKKIVRHYPRTPVAMITAFGSIELAVEALKEGAFDFVSKPVDLNVLRGMVRHAL
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHHH
ELNNRERSVVSSQPQSPSQRLLGESSPMAQLRATIAKVARSQAPVYILGESGVGKELVAR
HHCCCHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHH
TIHEQSVRAAGAFVPVNCGAIPVDLMESEFFGHKKGSFTGAHTDKPGLFQSAGGGTLFLD
HHHHHHHHHCCCEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCEEEHH
EVAELPLHMQVKLLRAIQEKSIRPIGATVEVPVDVRILSATHQDLSDLIANGRFRHDLYY
HHHCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCEEEEHHHHHHHHHHHHCCCEEEEEEE
RINVIEIRVPPLRERAGDLPHLAAAIVARLAHTHGRAVPLLSPSALEALEHYSFPGNVRE
EEEEEEEECCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCHHH
LENILERALALTEDDHIGAADLHLPQGSLHGQGLPLPLVPTVLPIVPADASASVEGENTP
HHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCC
AALPSYMEQLERAAIQKALEENRWNKTKTAAKLGITFRALRYKLKKLGME
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1317379; 8097014; 7911557; 10984043 [H]