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The map label for this gene is folC [H]

Identifier: 28198759

GI number: 28198759

Start: 1061399

End: 1062724

Strand: Reverse

Name: folC [H]

Synonym: PD0854

Alternate gene names: 28198759

Gene position: 1062724-1061399 (Counterclockwise)

Preceding gene: 28198761

Following gene: 28198758

Centisome position: 42.17

GC content: 51.73

Gene sequence:

>1326_bases
TTGTCTTACCTATGGCTGTTAGTCACCATGACTGCACAAAAAACTCTTTCCGAATGGCTTGCATATATCCAACAACAACA
CCCGCAGTCAATCTCTATGGGCTTGGAGCGCGTGAGCGCTGTGGCCAAGTCAATGGGGTTGGTCCATCCGGCGCGGCACT
GCATTGTAGTCGGAGGGACTAATGGCAAAGGGTCGACAGTAGCGTTCATTGAGGCGATAGCGCGCGCGGCAGGTTGGAAA
GTCGGCACCTACACCTCACCCCACCTACTGCGGTACAACGAGCGAGTACGCATTGATGGCAGAGAGGTTGATGATGCACT
CCTGGTCGAAGCTTTCTCCAGGGTTGAAGTAGCACGCAAACAAACTGAACTGACTTTTTTTGAGTTTGGCACTTTGGCTG
CTTTCTGGTTATTTTCGCGCGCCGTGCTTGATTTGGCGATACTGGAAGTGGGACTAGGTGGGCGCCTGGATGCAGTCAAT
TTAGTCGATGCCGACGTGGCTGTAATTACCACTGTGGACATCGACCATACCGAATGGCTAGGTAGCGATCGCGAGGCCAT
CGGTAAAGAAAAAGCTGGCATTATTCGAAGTTGGAAGCCAGTGGTGCTGGGTGAGCTTGACCCGCCATCAAGCGTTCTGC
GCCATGCCTACTTACTTGGTGCCAATGCGATCCGCGCTGGTAGCGATTTTTTCTACGAAAAAATCAATGAAAAGCACTGG
CGCTGGTGGGATGTTTCACTATGCATGGAGTTACCCTTGCCTGCACTCAGTGCACCAATACAGCTAGCCAATGCAGCCTC
TGCAATTGCTGCGTTGCGTGCGCTGCCACGAACTCTACCATTTGAAGCTTGGAGCGACGGTATAGCAAAAGCAACGATTG
CTGGACGGTTACAAGCATTCCAGATAGGAGCTATTCAAGTCTTGCTTGATGTCGGTCACAACCCGCAAGCTGCACAGGTA
CTCGCTTCTGCGCTGCGTACTCAACCGATACAAGGTAACGTTACCCATGCAGTCTATGCTGCGCTGGTCGATAAGGATGT
GCTAGGAGTCGCTACTGCTCTGGCTGAGATAGTGACACATTGGCACTTGGCTGGGCTGGATTGCTTACGTGGGCAATCGG
CTGAATGTTTGGCTGGACGACTTGCAGAAATATCAGTGGTACCTTCCAGCCTTTCCGGAAGCGTTACCGAAGCATTACAG
CAAACGTTACAAACTGCGGCTCCAGGTGACCGGGTACTTGTATTCGGCTCGTTCCATACCGTAGCTGAGGCTTTGCTACT
GCTTGGTTCATTTGACAAGAGTGAAGCATTGCCAGTAGACGTATAA

Upstream 100 bases:

>100_bases
AGGAAATCGTGATTCGTGACGCATAAAAAAGGGGCCAAAAAATAATTTCCATTAAAGAATTCTACCTTTTTACTAAAAGG
TATATAACAATCAGCTGCGT

Downstream 100 bases:

>100_bases
TCTATAACGCTTTAATTTTAGTGTGGTGCTTTCCAACAGTGGACATTGCTTTGAAACAGCGACTGATCGGTGCCGTCATT
CTAGTGGCGCTCGCCGTAAT

Product: folylpolyglutamate synthase/dihydrofolate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase [H]

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MSYLWLLVTMTAQKTLSEWLAYIQQQHPQSISMGLERVSAVAKSMGLVHPARHCIVVGGTNGKGSTVAFIEAIARAAGWK
VGTYTSPHLLRYNERVRIDGREVDDALLVEAFSRVEVARKQTELTFFEFGTLAAFWLFSRAVLDLAILEVGLGGRLDAVN
LVDADVAVITTVDIDHTEWLGSDREAIGKEKAGIIRSWKPVVLGELDPPSSVLRHAYLLGANAIRAGSDFFYEKINEKHW
RWWDVSLCMELPLPALSAPIQLANAASAIAALRALPRTLPFEAWSDGIAKATIAGRLQAFQIGAIQVLLDVGHNPQAAQV
LASALRTQPIQGNVTHAVYAALVDKDVLGVATALAEIVTHWHLAGLDCLRGQSAECLAGRLAEISVVPSSLSGSVTEALQ
QTLQTAAPGDRVLVFGSFHTVAEALLLLGSFDKSEALPVDV

Sequences:

>Translated_441_residues
MSYLWLLVTMTAQKTLSEWLAYIQQQHPQSISMGLERVSAVAKSMGLVHPARHCIVVGGTNGKGSTVAFIEAIARAAGWK
VGTYTSPHLLRYNERVRIDGREVDDALLVEAFSRVEVARKQTELTFFEFGTLAAFWLFSRAVLDLAILEVGLGGRLDAVN
LVDADVAVITTVDIDHTEWLGSDREAIGKEKAGIIRSWKPVVLGELDPPSSVLRHAYLLGANAIRAGSDFFYEKINEKHW
RWWDVSLCMELPLPALSAPIQLANAASAIAALRALPRTLPFEAWSDGIAKATIAGRLQAFQIGAIQVLLDVGHNPQAAQV
LASALRTQPIQGNVTHAVYAALVDKDVLGVATALAEIVTHWHLAGLDCLRGQSAECLAGRLAEISVVPSSLSGSVTEALQ
QTLQTAAPGDRVLVFGSFHTVAEALLLLGSFDKSEALPVDV
>Mature_440_residues
SYLWLLVTMTAQKTLSEWLAYIQQQHPQSISMGLERVSAVAKSMGLVHPARHCIVVGGTNGKGSTVAFIEAIARAAGWKV
GTYTSPHLLRYNERVRIDGREVDDALLVEAFSRVEVARKQTELTFFEFGTLAAFWLFSRAVLDLAILEVGLGGRLDAVNL
VDADVAVITTVDIDHTEWLGSDREAIGKEKAGIIRSWKPVVLGELDPPSSVLRHAYLLGANAIRAGSDFFYEKINEKHWR
WWDVSLCMELPLPALSAPIQLANAASAIAALRALPRTLPFEAWSDGIAKATIAGRLQAFQIGAIQVLLDVGHNPQAAQVL
ASALRTQPIQGNVTHAVYAALVDKDVLGVATALAEIVTHWHLAGLDCLRGQSAECLAGRLAEISVVPSSLSGSVTEALQQ
TLQTAAPGDRVLVFGSFHTVAEALLLLGSFDKSEALPVDV

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=169, Percent_Identity=37.8698224852071, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI66932984, Length=169, Percent_Identity=37.8698224852071, Blast_Score=113, Evalue=4e-25,
Organism=Escherichia coli, GI1788654, Length=418, Percent_Identity=44.9760765550239, Blast_Score=312, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI17553148, Length=188, Percent_Identity=35.6382978723404, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI71984923, Length=188, Percent_Identity=35.6382978723404, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17553150, Length=188, Percent_Identity=35.6382978723404, Blast_Score=114, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6323760, Length=319, Percent_Identity=26.6457680250784, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324815, Length=175, Percent_Identity=32, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24641571, Length=285, Percent_Identity=29.1228070175439, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24581568, Length=145, Percent_Identity=37.2413793103448, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47547; Mature: 47416

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYLWLLVTMTAQKTLSEWLAYIQQQHPQSISMGLERVSAVAKSMGLVHPARHCIVVGGT
CCEEEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
NGKGSTVAFIEAIARAAGWKVGTYTSPHLLRYNERVRIDGREVDDALLVEAFSRVEVARK
CCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCEEEECCCCCHHHHHHHHHHHHHHHHH
QTELTFFEFGTLAAFWLFSRAVLDLAILEVGLGGRLDAVNLVDADVAVITTVDIDHTEWL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEEECCCHHHC
GSDREAIGKEKAGIIRSWKPVVLGELDPPSSVLRHAYLLGANAIRAGSDFFYEKINEKHW
CCCHHHHCCHHHCHHCCCCCEEEECCCCHHHHHHHHHHHCCCHHHCCCHHHHHHHCCCCC
RWWDVSLCMELPLPALSAPIQLANAASAIAALRALPRTLPFEAWSDGIAKATIAGRLQAF
EEEEHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHE
QIGAIQVLLDVGHNPQAAQVLASALRTQPIQGNVTHAVYAALVDKDVLGVATALAEIVTH
EHHHHEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
WHLAGLDCLRGQSAECLAGRLAEISVVPSSLSGSVTEALQQTLQTAAPGDRVLVFGSFHT
HHHHCHHHHCCCCHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECHHH
VAEALLLLGSFDKSEALPVDV
HHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SYLWLLVTMTAQKTLSEWLAYIQQQHPQSISMGLERVSAVAKSMGLVHPARHCIVVGGT
CEEEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
NGKGSTVAFIEAIARAAGWKVGTYTSPHLLRYNERVRIDGREVDDALLVEAFSRVEVARK
CCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCEEEECCCCCHHHHHHHHHHHHHHHHH
QTELTFFEFGTLAAFWLFSRAVLDLAILEVGLGGRLDAVNLVDADVAVITTVDIDHTEWL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEEECCCHHHC
GSDREAIGKEKAGIIRSWKPVVLGELDPPSSVLRHAYLLGANAIRAGSDFFYEKINEKHW
CCCHHHHCCHHHCHHCCCCCEEEECCCCHHHHHHHHHHHCCCHHHCCCHHHHHHHCCCCC
RWWDVSLCMELPLPALSAPIQLANAASAIAALRALPRTLPFEAWSDGIAKATIAGRLQAF
EEEEHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHE
QIGAIQVLLDVGHNPQAAQVLASALRTQPIQGNVTHAVYAALVDKDVLGVATALAEIVTH
EHHHHEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
WHLAGLDCLRGQSAECLAGRLAEISVVPSSLSGSVTEALQQTLQTAAPGDRVLVFGSFHT
HHHHCHHHHCCCCHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECHHH
VAEALLLLGSFDKSEALPVDV
HHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]