Click here to switch to the map view.

The map label for this gene is pfkA [H]

Identifier: 28198149

GI number: 28198149

Start: 282692

End: 283975

Strand: Reverse

Name: pfkA [H]

Synonym: PD0221

Alternate gene names: 28198149

Gene position: 283975-282692 (Counterclockwise)

Preceding gene: 28198152

Following gene: 28198148

Centisome position: 11.27

GC content: 55.45

Gene sequence:

>1284_bases
ATGTCCTCGCACTACAAGAGCTCGTCCCACATGGCAAAAGAGACGTTGTTATACGCCCAATCCGGCGGCGTTACCGCTGT
TATCAACGCAACCGCCGCCGCGGTCATCACCGAAGCACGGTCACACCGCATTAAAGTCCTCGCCGCACGCAATGGCATCC
TCGGTGCACTGCGTGAAGACCTGATCGATACATCCAAAGAATCAATCACGGCAATCCGTGCCTTGACCCATACCCCAGGA
GGCGCATTTGGCTCGTGCCGTTTCAAGCTCAAATCGCTTGAAGCCGATCACGAAAAATACGAACGGTTACTTGAAGTATT
CAAAGCACATAACGTGCGCTGGTTCCTCTACAACGGTGGTAATGACTCCGCCGATACAGCGCTCAAAGTCTCACAGTTAG
CCAAAACATTTGACTATCCACTGCACTGTATCGGCGTCCCTAAAACCATCGACAACGATTTGGCGATCACCGACGCCAGC
CCCGGCTTCGGTTCAGCCGCAAAGTACACTGCTGTCTCCATATGCGAAGCCGCGCGGGATGTCGCAGCGATGGCCGAGAC
CTCAACTAAGGTTTTCATTTACGAAGCAATGGGCCGCCATGCCGGTTGGCTCGCTGCGGCTGCTGGCTTGGCCGGCCAAG
GGCAGGAAGAAGCCCCCCACATCATCCTGCTCCCTGAACGCACCTACGACGAAACAGCATTTCTGGCGAAAGTCCAACAG
GTAGTCGACCGAGTCGGTTGGTGCGTAGTAGTCGCCAGCGAAGGTATCCGCAGCAATGATGGGCGCTTCGTTGCCGATGC
TGGCGGCACCACTGATGCCTTTGGTCACACGCAACTTGGCGGCGTCGCCGCTTACCTCGCCAACCGAGTGAAACAGGCAC
TCGGACTCAAAGTGCACTGGACGCTGCCAGATTACCTACAACGCTCCGCAAGGCACATCGCCTCCAAAACAGACTGGGAA
CAAGCACAGGCTGTGGGTAAAGCTGCAGTGCACTACGCACTGGAAGGCATGAATGCAGTCATGCCTGTCATCGTACGCAC
CTCTGACACCCCATACCGTTGGAAAATCGAGCCGGCAGCACTCACCAAGATTGCCAATCACGAAAAAACCTTACCAGCAA
GCTTCTTACGCCGTGACGGCTTCGGCATCAGCGAACGTGCACGCACCTACTTTGAACCATTGATTCGTGGCGAAGCACCC
ATGCCCTACGAACGCAACGGATTACCGCGACACGTCCATCTGAAAAATCAAAGCGTACAGAAACGGCTACCTGCCTGGGA
ATAA

Upstream 100 bases:

>100_bases
ATAGAAAAAAAACAACGGCAAGCGGGGTTACACTTCGACCCTCTGCCTACACCGCACCATGATGCCGCGCAGCTTAACGC
ATCCAAGCGATGTCCCGGCA

Downstream 100 bases:

>100_bases
CACCCCTCACTGGTCACCTACAGTCTGCTTACTGACTTGAAACGTCATTGAAGGCCCAATCACCGAAAATTCCGCTTGCT
TCCAGCAAGCGGTGATAGCG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: 6-phosphofructokinase, pyrophosphate dependent; PPi-dependent phosphofructokinase; PPi-PFK; Pyrophosphate-dependent 6-phosphofructose-1-kinase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSSHYKSSSHMAKETLLYAQSGGVTAVINATAAAVITEARSHRIKVLAARNGILGALREDLIDTSKESITAIRALTHTPG
GAFGSCRFKLKSLEADHEKYERLLEVFKAHNVRWFLYNGGNDSADTALKVSQLAKTFDYPLHCIGVPKTIDNDLAITDAS
PGFGSAAKYTAVSICEAARDVAAMAETSTKVFIYEAMGRHAGWLAAAAGLAGQGQEEAPHIILLPERTYDETAFLAKVQQ
VVDRVGWCVVVASEGIRSNDGRFVADAGGTTDAFGHTQLGGVAAYLANRVKQALGLKVHWTLPDYLQRSARHIASKTDWE
QAQAVGKAAVHYALEGMNAVMPVIVRTSDTPYRWKIEPAALTKIANHEKTLPASFLRRDGFGISERARTYFEPLIRGEAP
MPYERNGLPRHVHLKNQSVQKRLPAWE

Sequences:

>Translated_427_residues
MSSHYKSSSHMAKETLLYAQSGGVTAVINATAAAVITEARSHRIKVLAARNGILGALREDLIDTSKESITAIRALTHTPG
GAFGSCRFKLKSLEADHEKYERLLEVFKAHNVRWFLYNGGNDSADTALKVSQLAKTFDYPLHCIGVPKTIDNDLAITDAS
PGFGSAAKYTAVSICEAARDVAAMAETSTKVFIYEAMGRHAGWLAAAAGLAGQGQEEAPHIILLPERTYDETAFLAKVQQ
VVDRVGWCVVVASEGIRSNDGRFVADAGGTTDAFGHTQLGGVAAYLANRVKQALGLKVHWTLPDYLQRSARHIASKTDWE
QAQAVGKAAVHYALEGMNAVMPVIVRTSDTPYRWKIEPAALTKIANHEKTLPASFLRRDGFGISERARTYFEPLIRGEAP
MPYERNGLPRHVHLKNQSVQKRLPAWE
>Mature_426_residues
SSHYKSSSHMAKETLLYAQSGGVTAVINATAAAVITEARSHRIKVLAARNGILGALREDLIDTSKESITAIRALTHTPGG
AFGSCRFKLKSLEADHEKYERLLEVFKAHNVRWFLYNGGNDSADTALKVSQLAKTFDYPLHCIGVPKTIDNDLAITDASP
GFGSAAKYTAVSICEAARDVAAMAETSTKVFIYEAMGRHAGWLAAAAGLAGQGQEEAPHIILLPERTYDETAFLAKVQQV
VDRVGWCVVVASEGIRSNDGRFVADAGGTTDAFGHTQLGGVAAYLANRVKQALGLKVHWTLPDYLQRSARHIASKTDWEQ
AQAVGKAAVHYALEGMNAVMPVIVRTSDTPYRWKIEPAALTKIANHEKTLPASFLRRDGFGISERARTYFEPLIRGEAPM
PYERNGLPRHVHLKNQSVQKRLPAWE

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Escherichia coli, GI1790350, Length=221, Percent_Identity=25.3393665158371, Blast_Score=69, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6321679, Length=214, Percent_Identity=30.3738317757009, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.90 [H]

Molecular weight: Translated: 46564; Mature: 46433

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSHYKSSSHMAKETLLYAQSGGVTAVINATAAAVITEARSHRIKVLAARNGILGALRED
CCCCCCCHHHHHHHHEEEECCCCEEEEEEHHHHHHHHHHCCCEEEEEEECCCHHHHHHHH
LIDTSKESITAIRALTHTPGGAFGSCRFKLKSLEADHEKYERLLEVFKAHNVRWFLYNGG
HHHCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEECC
NDSADTALKVSQLAKTFDYPLHCIGVPKTIDNDLAITDASPGFGSAAKYTAVSICEAARD
CCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHH
VAAMAETSTKVFIYEAMGRHAGWLAAAAGLAGQGQEEAPHIILLPERTYDETAFLAKVQQ
HHHHHHCCCEEEEEECCCCCCHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHH
VVDRVGWCVVVASEGIRSNDGRFVADAGGTTDAFGHTQLGGVAAYLANRVKQALGLKVHW
HHHHCCEEEEEECCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEE
TLPDYLQRSARHIASKTDWEQAQAVGKAAVHYALEGMNAVMPVIVRTSDTPYRWKIEPAA
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEECHHH
LTKIANHEKTLPASFLRRDGFGISERARTYFEPLIRGEAPMPYERNGLPRHVHLKNQSVQ
HHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHH
KRLPAWE
HCCCCCC
>Mature Secondary Structure 
SSHYKSSSHMAKETLLYAQSGGVTAVINATAAAVITEARSHRIKVLAARNGILGALRED
CCCCCCHHHHHHHHEEEECCCCEEEEEEHHHHHHHHHHCCCEEEEEEECCCHHHHHHHH
LIDTSKESITAIRALTHTPGGAFGSCRFKLKSLEADHEKYERLLEVFKAHNVRWFLYNGG
HHHCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEECC
NDSADTALKVSQLAKTFDYPLHCIGVPKTIDNDLAITDASPGFGSAAKYTAVSICEAARD
CCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHH
VAAMAETSTKVFIYEAMGRHAGWLAAAAGLAGQGQEEAPHIILLPERTYDETAFLAKVQQ
HHHHHHCCCEEEEEECCCCCCHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHH
VVDRVGWCVVVASEGIRSNDGRFVADAGGTTDAFGHTQLGGVAAYLANRVKQALGLKVHW
HHHHCCEEEEEECCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEE
TLPDYLQRSARHIASKTDWEQAQAVGKAAVHYALEGMNAVMPVIVRTSDTPYRWKIEPAA
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEECHHH
LTKIANHEKTLPASFLRRDGFGISERARTYFEPLIRGEAPMPYERNGLPRHVHLKNQSVQ
HHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHH
KRLPAWE
HCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10913106 [H]