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The map label for this gene is dnaX [C]

Identifier: 62185124

GI number: 62185124

Start: 574467

End: 575267

Strand: Direct

Name: dnaX [C]

Synonym: CAB496

Alternate gene names: 62185124

Gene position: 574467-575267 (Clockwise)

Preceding gene: 62185123

Following gene: 62185126

Centisome position: 50.2

GC content: 37.33

Gene sequence:

>801_bases
TTGCACGGAAGCTCGTTATCGATTCTTTCTCAATATGCTTACACCTTAGCCTCTCATATTCTGCTAAGAGATACCCCTGA
AGCACAGTATAAGATTTCCCAAAAAATACATCCTGATATTCAAGAGTTTCTGCCTTCTGGAAAGGGACGATTGCATTCTA
TAGAGATTCCTAGGGACATTAAAAAACAGATCGCCATACTTCCCTATGAAGGCCACTATAAGATTTATATTATTCATGAA
GTAGATCGTATGACTTTACCTGCGATCTCCGTATTTTTAAAAGTCCTCGAAGAGGCTCCATCTCACAATGTAATTCTACT
GACGTCTGCAAAACTTCAACGTATACCAGCAACGATTCTTTCACGGAGTTTATCCATACATATTCAAGGACAAGACAAGA
CTCTCCCTAATGAAGAAGAAATCGCCTATCTACTCAAGTATGCCTCAGGAGAAATGAGTATTACAGAAGTAGGAAAAATA
GTTAAAGGTAGTGTAGATACAGATAAACAAGTGCTCAGAGATAAAGCTAAATACCTTTTAGAGGTTCTTTTAACATTATT
TAGAGATAGATTTATACTTTCTTTAAATGTAAGTGCGAGTGCTATGACTTATCCGCAATACGCTAAAGGTATTCTTAATC
TGCCTGTACTTCCTCTTGAAAAGGTACTTGTGATTATAGAAAAAGCCTATCAAGCTTTGGATAATTCTTCATCAGCGACA
AGCTGTATGGAATGGGTAGCCTTACAACTTGCTTCACTGAATCATCGCTCTTCGATATTAACTGAACGTACTTGTCCATA
G

Upstream 100 bases:

>100_bases
CAGAATTAGCCTTATGCAAGTCGAAGGAAAGATAACGAATAAACCTTGGGAAGCTTTACTTGATAACATTAATCAAGGTA
AGGTAAGCCATGCTATTTTA

Downstream 100 bases:

>100_bases
GGGAAGGGTTTCCTGCATTTTTTGTAAAACAAGAGCAGGAATTTCATGCCCGCCATTAAAAGAAATAAACTCTCCATGTA
AACGTGAAGATAAGAGCTTG

Product: DNA polymerase III subunit delta'

Products: diphosphate; DNAn+1

Alternate protein names: DNA Polymerase III Gamma/Tau Subunit; DNA Polymerase III Gamma And Tau Subunits; DNA-Directed DNA Polymerase Subunit Delta; DNA Polymerase III Delta Subunit HolB; DNA Polymerase III Delta Prime Subunit; DNA Polymerase III Gamma/Tau Subunits-Like Protein; DNA Polymerase III Subunit Gamma/Tau; DNA Polymerase III Gamma-Tau; DNA Polymerase III Delta Subunit

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MHGSSLSILSQYAYTLASHILLRDTPEAQYKISQKIHPDIQEFLPSGKGRLHSIEIPRDIKKQIAILPYEGHYKIYIIHE
VDRMTLPAISVFLKVLEEAPSHNVILLTSAKLQRIPATILSRSLSIHIQGQDKTLPNEEEIAYLLKYASGEMSITEVGKI
VKGSVDTDKQVLRDKAKYLLEVLLTLFRDRFILSLNVSASAMTYPQYAKGILNLPVLPLEKVLVIIEKAYQALDNSSSAT
SCMEWVALQLASLNHRSSILTERTCP

Sequences:

>Translated_266_residues
MHGSSLSILSQYAYTLASHILLRDTPEAQYKISQKIHPDIQEFLPSGKGRLHSIEIPRDIKKQIAILPYEGHYKIYIIHE
VDRMTLPAISVFLKVLEEAPSHNVILLTSAKLQRIPATILSRSLSIHIQGQDKTLPNEEEIAYLLKYASGEMSITEVGKI
VKGSVDTDKQVLRDKAKYLLEVLLTLFRDRFILSLNVSASAMTYPQYAKGILNLPVLPLEKVLVIIEKAYQALDNSSSAT
SCMEWVALQLASLNHRSSILTERTCP
>Mature_266_residues
MHGSSLSILSQYAYTLASHILLRDTPEAQYKISQKIHPDIQEFLPSGKGRLHSIEIPRDIKKQIAILPYEGHYKIYIIHE
VDRMTLPAISVFLKVLEEAPSHNVILLTSAKLQRIPATILSRSLSIHIQGQDKTLPNEEEIAYLLKYASGEMSITEVGKI
VKGSVDTDKQVLRDKAKYLLEVLLTLFRDRFILSLNVSASAMTYPQYAKGILNLPVLPLEKVLVIIEKAYQALDNSSSAT
SCMEWVALQLASLNHRSSILTERTCP

Specific function: DNA Polymerase III Is A Complex, Multichain Enzyme Responsible For Most Of The Replicative Synthesis In Bacteria. This DNA Polymerase Also Exhibits 3' To 5' Exonuclease Activity. The Tau Chain Serves As A Scaffold To Help In The Dimerizaton Of The Core Co

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=174, Percent_Identity=28.1609195402299, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.7

Molecular weight: Translated: 29863; Mature: 29863

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGSSLSILSQYAYTLASHILLRDTPEAQYKISQKIHPDIQEFLPSGKGRLHSIEIPRDI
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHCCCCCCCEEEEECCHHH
KKQIAILPYEGHYKIYIIHEVDRMTLPAISVFLKVLEEAPSHNVILLTSAKLQRIPATIL
HHCEEEEEECCCEEEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHH
SRSLSIHIQGQDKTLPNEEEIAYLLKYASGEMSITEVGKIVKGSVDTDKQVLRDKAKYLL
CCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHH
EVLLTLFRDRFILSLNVSASAMTYPQYAKGILNLPVLPLEKVLVIIEKAYQALDNSSSAT
HHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHH
SCMEWVALQLASLNHRSSILTERTCP
HHHHHHHHHHHHCCHHHHHHHCCCCC
>Mature Secondary Structure
MHGSSLSILSQYAYTLASHILLRDTPEAQYKISQKIHPDIQEFLPSGKGRLHSIEIPRDI
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHCCCCCCCEEEEECCHHH
KKQIAILPYEGHYKIYIIHEVDRMTLPAISVFLKVLEEAPSHNVILLTSAKLQRIPATIL
HHCEEEEEECCCEEEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHH
SRSLSIHIQGQDKTLPNEEEIAYLLKYASGEMSITEVGKIVKGSVDTDKQVLRDKAKYLL
CCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHH
EVLLTLFRDRFILSLNVSASAMTYPQYAKGILNLPVLPLEKVLVIIEKAYQALDNSSSAT
HHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHH
SCMEWVALQLASLNHRSSILTERTCP
HHHHHHHHHHHHCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: deoxynucleoside triphosphate; DNAn

Specific reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA