Click here to switch to the map view.

The map label for this gene is ptsN [C]

Identifier: 62184971

GI number: 62184971

Start: 393043

End: 393723

Strand: Direct

Name: ptsN [C]

Synonym: CAB340

Alternate gene names: 62184971

Gene position: 393043-393723 (Clockwise)

Preceding gene: 62184970

Following gene: 62184972

Centisome position: 34.35

GC content: 38.62

Gene sequence:

>681_bases
ATGGATTTGAAATTAGAAGAGCTAGCTTCTTTGTTGGATATTTCTGAAAATACGGTCCGTCGGTGGCTAGATGAGGGAGC
TATCCCTAGTTACAGGATGAATAATGAACATAGATTTAATCGAGAAGAAATAGAAGATTGGATCCTCAATAATCAAGCGC
TACTAGGATTAGAAAAAGAAGAAAAAACAGACAAAGATTTTCGTGACCTGTCTTTGAAGTACAGCTTATATAAAGCCATT
TATCGCGGAGGCGTGATTCGCAATGTATCGGTAAAAAATAAAGCCGAAGCTTTGCAATACGCTTCTTCATACATAGCTGA
GAAATTTAATCTCGATGCGAGTGTTCTTTTCGAAATGCTCACTTATCGAGAAAGTCTTATGTCGACAGGTATCGGAGAAG
GCATCGCCCTCCCTCATGCTAAAGACTTTTTAATCAACGCGTACTACGATGTTGTTGTTCCTATGTTCCTAACCCACAGT
ATTGACTTCGGTGCTCTTGATGGGAAGCCTGTGTGTGTTTTGTTTTTCCTTTTCGCCTCCCAGGATAAAAGTCATTTAAA
TTTAATAAATAAAATCGTCCATCTCGGTATGTCTTTGGAAGCACGCAGTTTCCTGACAAATTATCCAGAAAAAGACCAGC
TCCTTGCTTACATTAAGAATTGGGAATCACAAATTCATTAA

Upstream 100 bases:

>100_bases
ATATTGACTCTTTCGCTTAGAGATGAATCTCGACGTCAAAAGTTGCTTCAAGTGACAACTATTGAAGAAGTTATGAATGT
GTTTTTAGGAATGTAGTGTT

Downstream 100 bases:

>100_bases
CTATCAATCTCTATAAAAGATTTGCAAGTCCAAGTTGTGAAAAAAGTCCTATTGTTGTTATGGTGATCTCATAGGCTGAT
CTAGGAGATTATAGCAGCAT

Product: PTS system, IIA component

Products: protein histidine; sugar phosphate

Alternate protein names: PTS System IIA Component; PtsN PTS System Nitrogen Regulatory IIA Component; MerR Family Helix-Turn-Helix Transcriptional Regulator; Phosphotransferase System II; PTS System Fructose Subfamily IIA Subunit; PTS System IIA Protein; Nitrogen Regulatory IIA Protein

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MDLKLEELASLLDISENTVRRWLDEGAIPSYRMNNEHRFNREEIEDWILNNQALLGLEKEEKTDKDFRDLSLKYSLYKAI
YRGGVIRNVSVKNKAEALQYASSYIAEKFNLDASVLFEMLTYRESLMSTGIGEGIALPHAKDFLINAYYDVVVPMFLTHS
IDFGALDGKPVCVLFFLFASQDKSHLNLINKIVHLGMSLEARSFLTNYPEKDQLLAYIKNWESQIH

Sequences:

>Translated_226_residues
MDLKLEELASLLDISENTVRRWLDEGAIPSYRMNNEHRFNREEIEDWILNNQALLGLEKEEKTDKDFRDLSLKYSLYKAI
YRGGVIRNVSVKNKAEALQYASSYIAEKFNLDASVLFEMLTYRESLMSTGIGEGIALPHAKDFLINAYYDVVVPMFLTHS
IDFGALDGKPVCVLFFLFASQDKSHLNLINKIVHLGMSLEARSFLTNYPEKDQLLAYIKNWESQIH
>Mature_226_residues
MDLKLEELASLLDISENTVRRWLDEGAIPSYRMNNEHRFNREEIEDWILNNQALLGLEKEEKTDKDFRDLSLKYSLYKAI
YRGGVIRNVSVKNKAEALQYASSYIAEKFNLDASVLFEMLTYRESLMSTGIGEGIALPHAKDFLINAYYDVVVPMFLTHS
IDFGALDGKPVCVLFFLFASQDKSHLNLINKIVHLGMSLEARSFLTNYPEKDQLLAYIKNWESQIH

Specific function: Seems To Have A Role In Linking Carbon And Nitrogen Assimilation. [C]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789597, Length=117, Percent_Identity=35.8974358974359, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.1.69

Molecular weight: Translated: 26067; Mature: 26067

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLKLEELASLLDISENTVRRWLDEGAIPSYRMNNEHRFNREEIEDWILNNQALLGLEKE
CCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEECCCHH
EKTDKDFRDLSLKYSLYKAIYRGGVIRNVSVKNKAEALQYASSYIAEKFNLDASVLFEML
HHCCHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
TYRESLMSTGIGEGIALPHAKDFLINAYYDVVVPMFLTHSIDFGALDGKPVCVLFFLFAS
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHC
QDKSHLNLINKIVHLGMSLEARSFLTNYPEKDQLLAYIKNWESQIH
CCHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDLKLEELASLLDISENTVRRWLDEGAIPSYRMNNEHRFNREEIEDWILNNQALLGLEKE
CCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEECCCHH
EKTDKDFRDLSLKYSLYKAIYRGGVIRNVSVKNKAEALQYASSYIAEKFNLDASVLFEML
HHCCHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
TYRESLMSTGIGEGIALPHAKDFLINAYYDVVVPMFLTHSIDFGALDGKPVCVLFFLFAS
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHC
QDKSHLNLINKIVHLGMSLEARSFLTNYPEKDQLLAYIKNWESQIH
CCHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA