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The map label for this gene is gatB

Identifier: 62184919

GI number: 62184919

Start: 333698

End: 335161

Strand: Direct

Name: gatB

Synonym: CAB287

Alternate gene names: 62184919

Gene position: 333698-335161 (Clockwise)

Preceding gene: 62184918

Following gene: 62184924

Centisome position: 29.16

GC content: 42.28

Gene sequence:

>1464_bases
ATGAGCGACGTTTATGCTGATTGGGAATCCGTCATAGGTCTTGAAGTCCACGTAGAATTAAACACAAAATCTAAATTGTT
CAGTTGTGCACGCAACCGTTTTGGAGACGAACCTAATACAAACATCTCTCCTGTATGCACCGGCATGCCGGGGTCACTGC
CAGTACTGAATAAAGAAGCAGTGAGAAAGGCTGTTTTATTTGGTTGTGCTGTTGAAGGCGAAGTAGCTTTGCTCAGCCGT
TTTGATAGAAAGTCCTATTTTTATCCCGATAGCCCAAGGAATTTTCAAATTACCCAATTCGAACATCCTATTGTGCGAGG
AGGACATATAAAAGCTATCGTTCACGGTGAGGAACGTCATTTTGAACTGGCTCAAGCGCATATCGAAGATGATGCCGGTA
TGCTAAAACATTTCGGAGAATTTGCTGGAGTAGATTATAACCGCGCTGGTGTACCTTTAATAGAGATTGTGTCTAAGCCG
TGCATGTTTTGTGCTGATGATGCTGTTGCTTATGCCACAGCTTTGGTATCCTTATTAGACTACATAGGCATTTCTGACTG
TAATATGGAAGAAGGCTCGGTACGCTTTGATGTAAACATATCCGTACGTCCTAAAGGTAGCGAAGAACTACGCAATAAAG
TAGAAATTAAAAATATGAACTCCTTTGCTTTTATGGCCCAAGCTCTAGAAGCCGAGCGTTGTCGTCAGATCGATGCATAT
TTAGACAATCCAAATGCAGACCCCAAAACTGTTATTCCAGGAGCGACATACCGTTGGGATCCTGAAAAGAAAAAAACAGT
GTTGATGCGTCTTAAGGAACGAGCTGAAGATTACAAGTATTTCATAGAGCCTGATCTCCCAGTATTGCAATTAACAGAAG
CATATATTGATGAAATTCGTCATACGCTTCCCGAGCTCCCTTTCAACAAATACCAAAGGTATTTGCACGAATATGCTCTT
GCCGAAGACATCGCTGCCATTTTAATTAGCGATAAGCATAGTGCGCACTTCTTTGAATTAGCCGCTCAGGAATGTAAAAA
CTACAGAGCCCTTTCTAATTGGTTAACTGTTGAGTTTGCCGGACGTTGTAAACTCAAGGGTAAGAATCTCGCTTTCTCAG
GTATCCTGCCCAGTAGTGTAGCTCAGCTTGTGAATTTTATTGATCAAGGCGTGATTACCGGAAAGATCGCTAAGGATATC
GCAGACATGATGATGGAATCTCCTGAAAAGAGTCCTGAGACTATCCTCAAAGAAAATCCTGAAATGTTGCCCATGACAGA
TGAAAGTGCGTTGGTGGCGATCATTTCCGAGGTGATTACCGCAAATCCGCAGTCTGTCGTAGACTACAAAAGTGGTAAGA
CCAAGGCGTTAGGATTTTTAGTTGGGCAAATTATGAAACGTACCCAGGGCAAGGCCCCTCCAAATAGGGTAAATGAACTT
TTGCTTGTGGAATTAAGTAAATAA

Upstream 100 bases:

>100_bases
AACAGGTGTGCCAGGTAGGCTATAGCTTCCAAGAACATTCAGGAATTAAGAATTTATACCCTAAAGGATGTAACAAACTT
GTTGATGGAGAGGTGAAATA

Downstream 100 bases:

>100_bases
TAATTGAAAAATCTTATCGTTTGTCTTATGCTATTGCGTATCTAAGGGAACGGAGGCTCTTTTGACGTTTTTAACGTCAA
AAGAGCTCTTTTATTTTGCC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MSDVYADWESVIGLEVHVELNTKSKLFSCARNRFGDEPNTNISPVCTGMPGSLPVLNKEAVRKAVLFGCAVEGEVALLSR
FDRKSYFYPDSPRNFQITQFEHPIVRGGHIKAIVHGEERHFELAQAHIEDDAGMLKHFGEFAGVDYNRAGVPLIEIVSKP
CMFCADDAVAYATALVSLLDYIGISDCNMEEGSVRFDVNISVRPKGSEELRNKVEIKNMNSFAFMAQALEAERCRQIDAY
LDNPNADPKTVIPGATYRWDPEKKKTVLMRLKERAEDYKYFIEPDLPVLQLTEAYIDEIRHTLPELPFNKYQRYLHEYAL
AEDIAAILISDKHSAHFFELAAQECKNYRALSNWLTVEFAGRCKLKGKNLAFSGILPSSVAQLVNFIDQGVITGKIAKDI
ADMMMESPEKSPETILKENPEMLPMTDESALVAIISEVITANPQSVVDYKSGKTKALGFLVGQIMKRTQGKAPPNRVNEL
LLVELSK

Sequences:

>Translated_487_residues
MSDVYADWESVIGLEVHVELNTKSKLFSCARNRFGDEPNTNISPVCTGMPGSLPVLNKEAVRKAVLFGCAVEGEVALLSR
FDRKSYFYPDSPRNFQITQFEHPIVRGGHIKAIVHGEERHFELAQAHIEDDAGMLKHFGEFAGVDYNRAGVPLIEIVSKP
CMFCADDAVAYATALVSLLDYIGISDCNMEEGSVRFDVNISVRPKGSEELRNKVEIKNMNSFAFMAQALEAERCRQIDAY
LDNPNADPKTVIPGATYRWDPEKKKTVLMRLKERAEDYKYFIEPDLPVLQLTEAYIDEIRHTLPELPFNKYQRYLHEYAL
AEDIAAILISDKHSAHFFELAAQECKNYRALSNWLTVEFAGRCKLKGKNLAFSGILPSSVAQLVNFIDQGVITGKIAKDI
ADMMMESPEKSPETILKENPEMLPMTDESALVAIISEVITANPQSVVDYKSGKTKALGFLVGQIMKRTQGKAPPNRVNEL
LLVELSK
>Mature_486_residues
SDVYADWESVIGLEVHVELNTKSKLFSCARNRFGDEPNTNISPVCTGMPGSLPVLNKEAVRKAVLFGCAVEGEVALLSRF
DRKSYFYPDSPRNFQITQFEHPIVRGGHIKAIVHGEERHFELAQAHIEDDAGMLKHFGEFAGVDYNRAGVPLIEIVSKPC
MFCADDAVAYATALVSLLDYIGISDCNMEEGSVRFDVNISVRPKGSEELRNKVEIKNMNSFAFMAQALEAERCRQIDAYL
DNPNADPKTVIPGATYRWDPEKKKTVLMRLKERAEDYKYFIEPDLPVLQLTEAYIDEIRHTLPELPFNKYQRYLHEYALA
EDIAAILISDKHSAHFFELAAQECKNYRALSNWLTVEFAGRCKLKGKNLAFSGILPSSVAQLVNFIDQGVITGKIAKDIA
DMMMESPEKSPETILKENPEMLPMTDESALVAIISEVITANPQSVVDYKSGKTKALGFLVGQIMKRTQGKAPPNRVNELL
LVELSK

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=502, Percent_Identity=33.4661354581673, Blast_Score=246, Evalue=3e-65,
Organism=Caenorhabditis elegans, GI115534094, Length=306, Percent_Identity=36.2745098039216, Blast_Score=167, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6319391, Length=509, Percent_Identity=29.4695481335953, Blast_Score=186, Evalue=6e-48,
Organism=Drosophila melanogaster, GI45550808, Length=486, Percent_Identity=29.2181069958848, Blast_Score=205, Evalue=7e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_CHLAB (Q5L6I8)

Other databases:

- EMBL:   CR848038
- RefSeq:   YP_219704.1
- ProteinModelPortal:   Q5L6I8
- SMR:   Q5L6I8
- GeneID:   3338053
- GenomeReviews:   CR848038_GR
- KEGG:   cab:CAB287
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   CABO218497:CAB287-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 54565; Mature: 54434

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVYADWESVIGLEVHVELNTKSKLFSCARNRFGDEPNTNISPVCTGMPGSLPVLNKEA
CCCCHHCHHHEEEEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCHHH
VRKAVLFGCAVEGEVALLSRFDRKSYFYPDSPRNFQITQFEHPIVRGGHIKAIVHGEERH
HHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEEECCHHH
FELAQAHIEDDAGMLKHFGEFAGVDYNRAGVPLIEIVSKPCMFCADDAVAYATALVSLLD
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCHHCCHHHHHHHHHHHHHHH
YIGISDCNMEEGSVRFDVNISVRPKGSEELRNKVEIKNMNSFAFMAQALEAERCRQIDAY
HCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
LDNPNADPKTVIPGATYRWDPEKKKTVLMRLKERAEDYKYFIEPDLPVLQLTEAYIDEIR
CCCCCCCCCEECCCCCEEECCHHHHHHHHHHHHHHHHCEEEECCCCCHHHHHHHHHHHHH
HTLPELPFNKYQRYLHEYALAEDIAAILISDKHSAHFFELAAQECKNYRALSNWLTVEFA
HHCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHEEEEEEC
GRCKLKGKNLAFSGILPSSVAQLVNFIDQGVITGKIAKDIADMMMESPEKSPETILKENP
CEEEECCCCEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHCCC
EMLPMTDESALVAIISEVITANPQSVVDYKSGKTKALGFLVGQIMKRTQGKAPPNRVNEL
CCCCCCCCHHHHHHHHHHHHCCCHHHEECCCCCHHHHHHHHHHHHHHHCCCCCHHHCCEE
LLVELSK
EEEEECC
>Mature Secondary Structure 
SDVYADWESVIGLEVHVELNTKSKLFSCARNRFGDEPNTNISPVCTGMPGSLPVLNKEA
CCCHHCHHHEEEEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCHHH
VRKAVLFGCAVEGEVALLSRFDRKSYFYPDSPRNFQITQFEHPIVRGGHIKAIVHGEERH
HHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEEECCHHH
FELAQAHIEDDAGMLKHFGEFAGVDYNRAGVPLIEIVSKPCMFCADDAVAYATALVSLLD
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCHHCCHHHHHHHHHHHHHHH
YIGISDCNMEEGSVRFDVNISVRPKGSEELRNKVEIKNMNSFAFMAQALEAERCRQIDAY
HCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
LDNPNADPKTVIPGATYRWDPEKKKTVLMRLKERAEDYKYFIEPDLPVLQLTEAYIDEIR
CCCCCCCCCEECCCCCEEECCHHHHHHHHHHHHHHHHCEEEECCCCCHHHHHHHHHHHHH
HTLPELPFNKYQRYLHEYALAEDIAAILISDKHSAHFFELAAQECKNYRALSNWLTVEFA
HHCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHEEEEEEC
GRCKLKGKNLAFSGILPSSVAQLVNFIDQGVITGKIAKDIADMMMESPEKSPETILKENP
CEEEECCCCEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHCCC
EMLPMTDESALVAIISEVITANPQSVVDYKSGKTKALGFLVGQIMKRTQGKAPPNRVNEL
CCCCCCCCHHHHHHHHHHHHCCCHHHEECCCCCHHHHHHHHHHHHHHHCCCCCHHHCCEE
LLVELSK
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA