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The map label for this gene is gatA

Identifier: 62184918

GI number: 62184918

Start: 332223

End: 333698

Strand: Direct

Name: gatA

Synonym: CAB286

Alternate gene names: 62184918

Gene position: 332223-333698 (Clockwise)

Preceding gene: 62184917

Following gene: 62184919

Centisome position: 29.03

GC content: 43.29

Gene sequence:

>1476_bases
ATGTATCAGAAGAGTGCCTTAGAGTTAAGAAATGCCGTAGTGAGTGGAGAGTCTTCAGCTACAGCAATAGCAAAGTATTT
TTATAATAGAATAAAAACAGAAGACAATCAGATAGGAGCTTTTCTTTCTCTTTGTGAAGAAAGAGCTTATGAGAAAGCAG
CTATCATAGATGCGAAAGTGGCGCGAGGAGAACCTTTGGGGAAACTCGCAGGTGTCCCCATCGGGATAAAAGATAATATT
CATATTCGGGGTTTGCGCACCACTTGTGCTTCTAAAATGTTAGAAAATTATATAGCGCCTTTTGATGCTACAGTCGTCGA
ACGGATAGAAGCTGAAGATGGGGTCATTTTAGGCAAACTCAATATGGATGAGTTTGCTATGGGATCGACAACGCAGTATT
CTGCTTTCCATCCTACGAAAAATCCTTGGGGTTTATCCTGTGTGCCAGGAGGATCTTCAGGGGGATCCGCCGCCGCAGTT
TCTGCAAGATTTTGTCCTATAGCGTTAGGTTCGGATACCGGTGGATCTATACGTCAGCCAGCAGCATTTTGTGGAGTTGT
GGGGTTTAAGCCCTCCTATGGAGCCGTCTCCCGTTACGGTTTAGTCGCTTTTGGGTCTTCATTAGATCAGATAGGCCCTT
TAACAACAGTTGTCGAAGATGTCGCCTTAGCTATGGATGTATTCGCAGGTAAGGATGATAGAGATGCAACTTCTCAGAAG
TTTTTTACAGGATCTTTCCAAGAGGCCTTGTCTTTAGACGTTCCGAGTTTGATCGGCGTGCCTATGGGATTTTTAGACGG
TTTACGTGATGATGTTAAAGAGAATTTCTTTGCCTCTTTAAGTATTTTGGAACGTCAGGGTAGCCGCATTGTTGAAGTGG
ATCTTAACATCTTAGATCACGCTGTCTCTGTTTACTACATTGTCGCTTCTGCAGAAGCCGCAACAAATCTTGCAAGATTT
GATGGTATTCGTTACGGCTATCGTTCTCCAGAAGCGCATAGTATAGAAGATATTTATACGATCTCCCGCGTACAAGGCTT
CGGTAAGGAAGTCATGCGTAGGATTCTTTTAGGTAACTATGTGTTATCCACTGAGCGCCAAAATGTCTATTATAAGAAAG
GCTCCGCAATTCGAGCAAAAATCATTCAAGCTTTTCAAAAAGCTTATGAAAAGTGTGATGTGATTGCGATGCCTGTATGC
TCATGCCCAGCATTCGCCGATGGCGAAATCCTTGATCCTACCTCTCTATATCTCCAGGATATCTATACCGTGGCTATGAA
TTTAGCCTACCTCCCAGCTATCGCCGTTCCTTCAGGGTTTTCTCGAGAAGGGCTGCCTCTAGGATTCCAGGTGATTGGAC
AAAAGGGTAAAGATCAACAGGTGTGCCAGGTAGGCTATAGCTTCCAAGAACATTCAGGAATTAAGAATTTATACCCTAAA
GGATGTAACAAACTTGTTGATGGAGAGGTGAAATAA

Upstream 100 bases:

>100_bases
TTTCTCTCGTGAGGAGTTTCTAACTAATGTCCCTGAATCCTTAGGGGGATTAGTAAAAGTACCCACAGTCATTAAGTAGA
GATCACAGTTTTAGGAAGAT

Downstream 100 bases:

>100_bases
TGAGCGACGTTTATGCTGATTGGGAATCCGTCATAGGTCTTGAAGTCCACGTAGAATTAAACACAAAATCTAAATTGTTC
AGTTGTGCACGCAACCGTTT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MYQKSALELRNAVVSGESSATAIAKYFYNRIKTEDNQIGAFLSLCEERAYEKAAIIDAKVARGEPLGKLAGVPIGIKDNI
HIRGLRTTCASKMLENYIAPFDATVVERIEAEDGVILGKLNMDEFAMGSTTQYSAFHPTKNPWGLSCVPGGSSGGSAAAV
SARFCPIALGSDTGGSIRQPAAFCGVVGFKPSYGAVSRYGLVAFGSSLDQIGPLTTVVEDVALAMDVFAGKDDRDATSQK
FFTGSFQEALSLDVPSLIGVPMGFLDGLRDDVKENFFASLSILERQGSRIVEVDLNILDHAVSVYYIVASAEAATNLARF
DGIRYGYRSPEAHSIEDIYTISRVQGFGKEVMRRILLGNYVLSTERQNVYYKKGSAIRAKIIQAFQKAYEKCDVIAMPVC
SCPAFADGEILDPTSLYLQDIYTVAMNLAYLPAIAVPSGFSREGLPLGFQVIGQKGKDQQVCQVGYSFQEHSGIKNLYPK
GCNKLVDGEVK

Sequences:

>Translated_491_residues
MYQKSALELRNAVVSGESSATAIAKYFYNRIKTEDNQIGAFLSLCEERAYEKAAIIDAKVARGEPLGKLAGVPIGIKDNI
HIRGLRTTCASKMLENYIAPFDATVVERIEAEDGVILGKLNMDEFAMGSTTQYSAFHPTKNPWGLSCVPGGSSGGSAAAV
SARFCPIALGSDTGGSIRQPAAFCGVVGFKPSYGAVSRYGLVAFGSSLDQIGPLTTVVEDVALAMDVFAGKDDRDATSQK
FFTGSFQEALSLDVPSLIGVPMGFLDGLRDDVKENFFASLSILERQGSRIVEVDLNILDHAVSVYYIVASAEAATNLARF
DGIRYGYRSPEAHSIEDIYTISRVQGFGKEVMRRILLGNYVLSTERQNVYYKKGSAIRAKIIQAFQKAYEKCDVIAMPVC
SCPAFADGEILDPTSLYLQDIYTVAMNLAYLPAIAVPSGFSREGLPLGFQVIGQKGKDQQVCQVGYSFQEHSGIKNLYPK
GCNKLVDGEVK
>Mature_491_residues
MYQKSALELRNAVVSGESSATAIAKYFYNRIKTEDNQIGAFLSLCEERAYEKAAIIDAKVARGEPLGKLAGVPIGIKDNI
HIRGLRTTCASKMLENYIAPFDATVVERIEAEDGVILGKLNMDEFAMGSTTQYSAFHPTKNPWGLSCVPGGSSGGSAAAV
SARFCPIALGSDTGGSIRQPAAFCGVVGFKPSYGAVSRYGLVAFGSSLDQIGPLTTVVEDVALAMDVFAGKDDRDATSQK
FFTGSFQEALSLDVPSLIGVPMGFLDGLRDDVKENFFASLSILERQGSRIVEVDLNILDHAVSVYYIVASAEAATNLARF
DGIRYGYRSPEAHSIEDIYTISRVQGFGKEVMRRILLGNYVLSTERQNVYYKKGSAIRAKIIQAFQKAYEKCDVIAMPVC
SCPAFADGEILDPTSLYLQDIYTVAMNLAYLPAIAVPSGFSREGLPLGFQVIGQKGKDQQVCQVGYSFQEHSGIKNLYPK
GCNKLVDGEVK

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=498, Percent_Identity=35.3413654618474, Blast_Score=295, Evalue=7e-80,
Organism=Homo sapiens, GI195972892, Length=237, Percent_Identity=29.1139240506329, Blast_Score=102, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17543272, Length=409, Percent_Identity=36.6748166259169, Blast_Score=252, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI17537465, Length=499, Percent_Identity=24.4488977955912, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17556264, Length=235, Percent_Identity=29.3617021276596, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71990152, Length=457, Percent_Identity=24.507658643326, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=189, Percent_Identity=30.1587301587302, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=189, Percent_Identity=30.1587301587302, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17538252, Length=188, Percent_Identity=28.1914893617021, Blast_Score=66, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=424, Percent_Identity=35.377358490566, Blast_Score=212, Evalue=9e-56,
Organism=Saccharomyces cerevisiae, GI6319685, Length=419, Percent_Identity=30.0715990453461, Blast_Score=126, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24648113, Length=462, Percent_Identity=36.3636363636364, Blast_Score=268, Evalue=5e-72,
Organism=Drosophila melanogaster, GI24644968, Length=498, Percent_Identity=24.4979919678715, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24648435, Length=500, Percent_Identity=24.4, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24648437, Length=500, Percent_Identity=24.4, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI45550774, Length=500, Percent_Identity=24.4, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24648441, Length=435, Percent_Identity=25.0574712643678, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24648439, Length=435, Percent_Identity=25.0574712643678, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI161078093, Length=503, Percent_Identity=25.844930417495, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24652985, Length=496, Percent_Identity=24.7983870967742, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19922090, Length=496, Percent_Identity=24.7983870967742, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24652981, Length=496, Percent_Identity=24.7983870967742, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24652983, Length=496, Percent_Identity=24.7983870967742, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21356731, Length=235, Percent_Identity=29.7872340425532, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_CHLAB (Q5L6I9)

Other databases:

- EMBL:   CR848038
- RefSeq:   YP_219703.1
- ProteinModelPortal:   Q5L6I9
- SMR:   Q5L6I9
- GeneID:   3338052
- GenomeReviews:   CR848038_GR
- KEGG:   cab:CAB286
- HOGENOM:   HBG481888
- OMA:   TESSCYG
- ProtClustDB:   PRK00012
- BioCyc:   CABO218497:CAB286-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 53064; Mature: 53064

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 77-77 ACT_SITE 152-152 ACT_SITE 176-176

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQKSALELRNAVVSGESSATAIAKYFYNRIKTEDNQIGAFLSLCEERAYEKAAIIDAKV
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ARGEPLGKLAGVPIGIKDNIHIRGLRTTCASKMLENYIAPFDATVVERIEAEDGVILGKL
HCCCCCHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEC
NMDEFAMGSTTQYSAFHPTKNPWGLSCVPGGSSGGSAAAVSARFCPIALGSDTGGSIRQP
CCHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEECCCEEEECCCCCCCCCCC
AAFCGVVGFKPSYGAVSRYGLVAFGSSLDQIGPLTTVVEDVALAMDVFAGKDDRDATSQK
HHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHCHHHHHHHHHHHHHHHHCCCCCCCCHHHH
FFTGSFQEALSLDVPSLIGVPMGFLDGLRDDVKENFFASLSILERQGSRIVEVDLNILDH
HCCCCHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHH
AVSVYYIVASAEAATNLARFDGIRYGYRSPEAHSIEDIYTISRVQGFGKEVMRRILLGNY
HHEEEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCH
VLSTERQNVYYKKGSAIRAKIIQAFQKAYEKCDVIAMPVCSCPAFADGEILDPTSLYLQD
HEECCCCCEEEECCCHHHHHHHHHHHHHHHHCCEEEECHHCCCCCCCCCCCCHHHHHHHH
IYTVAMNLAYLPAIAVPSGFSREGLPLGFQVIGQKGKDQQVCQVGYSFQEHSGIKNLYPK
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHCCCHHHHCCCHHCCHH
GCNKLVDGEVK
HCCCCCCCCCC
>Mature Secondary Structure
MYQKSALELRNAVVSGESSATAIAKYFYNRIKTEDNQIGAFLSLCEERAYEKAAIIDAKV
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ARGEPLGKLAGVPIGIKDNIHIRGLRTTCASKMLENYIAPFDATVVERIEAEDGVILGKL
HCCCCCHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEC
NMDEFAMGSTTQYSAFHPTKNPWGLSCVPGGSSGGSAAAVSARFCPIALGSDTGGSIRQP
CCHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEECCCEEEECCCCCCCCCCC
AAFCGVVGFKPSYGAVSRYGLVAFGSSLDQIGPLTTVVEDVALAMDVFAGKDDRDATSQK
HHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHCHHHHHHHHHHHHHHHHCCCCCCCCHHHH
FFTGSFQEALSLDVPSLIGVPMGFLDGLRDDVKENFFASLSILERQGSRIVEVDLNILDH
HCCCCHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHH
AVSVYYIVASAEAATNLARFDGIRYGYRSPEAHSIEDIYTISRVQGFGKEVMRRILLGNY
HHEEEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCH
VLSTERQNVYYKKGSAIRAKIIQAFQKAYEKCDVIAMPVCSCPAFADGEILDPTSLYLQD
HEECCCCCEEEECCCHHHHHHHHHHHHHHHHCCEEEECHHCCCCCCCCCCCCHHHHHHHH
IYTVAMNLAYLPAIAVPSGFSREGLPLGFQVIGQKGKDQQVCQVGYSFQEHSGIKNLYPK
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHCCCHHHHCCCHHCCHH
GCNKLVDGEVK
HCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA