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The map label for this gene is pdhC [H]

Identifier: 28572938

GI number: 28572938

Start: 903614

End: 904936

Strand: Reverse

Name: pdhC [H]

Synonym: TW797

Alternate gene names: 28572938

Gene position: 904936-903614 (Counterclockwise)

Preceding gene: 28572939

Following gene: 28572937

Centisome position: 97.73

GC content: 49.66

Gene sequence:

>1323_bases
ATGCCAGAAGTTACTTTCCTTTTAACCGATCCGGGCGAAGGCTTGGTCGAGGCCGAAATTGTCTCCTTGAGGGTTACCGA
GGGTGATGCGGTTGATGTGAATCAGATTGTTGTTGAGGTGGAAACAGCAAAGTCTCTTGTTGAGCTCCCGTCACCCTTCA
AGGGTGTAGTTAGGAAACTGCTTGTCGCTGTGGGTGAACTTGTCAAGGTGGGCTCGCCGATAATGCTGATCGACACAGAC
GAAACCCCGCCTGCCGGGCTACCGAAGACCGGAAAGATGCCTGTTTCTGATGTGCGTGATGGCGGGCATGATACTGCCAA
AAACACTCATCCCCGCGATACAGGCTCATCTAAGGATATTCACGATGCATTCGGGAAAGAGGATGGTCGTGAGATTGATT
TCGGGCGGAATGTGCTTGTTGGGTATGGCCCGTGTGAGGATAATGCTAGGGTGCGAGGTGATGATATTACCGGGAGACCT
TCACTTGATGAAACCCTAGAAAGGCCAGTCGCTAAGCCTTCGGTCAGGAAATTTGCAAAGGAGCTTGGTGTCGATCTTTA
CGGTGTAAAACCGACCGGTATTGGTGGCACCATAACACGACGAGATGTTCTTAATGCTACTTCCACTCAGGAGGAAGCCA
CCACTCGGGTTCCTGTGAAAGGCCTGCGCCGACAGACAGCGCAGAATGTTACGCTCAGCGCATTTAGCGTTCCGCATGTA
AGTGTATTCGTAGATGTTGATGTGACCCGCACAATTGAACTGGTTGATCGCCTAAAAAGAGATCCGCTGTATGAACAGGT
TAGAATCTCCCCGCTTCTTATCTTGTCTCGTGCCGTTACCTGGGCTGTCAAGCGGAGTCCAATTGTCAACTCAACCTGGA
GCGAACAGGAAATTCTTCTTCATAAGAGTGTCAATTTGGGAATTGCAGTTGCAACAGACAGAGGTCTTGTTGTTCCCAAT
ATAAAAAATGCGCAGTGCCTCTCAATGCTGGACCTTGCAAAGGCTATAGAGAGTTTGGTTGATGATGCAAGGTCATCACG
CATTCGGCCCGAGGATACCCTGAATGGCACAATAACAATTACCAATATTGGGGTCTTTGGGGTGGATTCGGGTACTCCAA
TTTTGAATGTTGGCGAATCGTCCATTGTTTTTATTGGAGCCATAAAGCCCAGACCTTGGGTGGTTAATGGCGAGATTTCC
GTTCGGCGCGTTGCAACTATCGGGGGCAGTTTTGACCATCGTGTAATGGATGGTGACACCGCAAGTCGTTTTCTCGTGAA
TGTTGCCTCGATTCTTGAGGAGCCGGCACTTCTGGTCGAATAG

Upstream 100 bases:

>100_bases
TTCCTCCGGCAAAACTTGAGAAGGTCTATTTACCGGATGTTGATAGAGTCTTAACAGCAATTAAGGGTGTTATGTCCCAT
TAGGATGAGAAGAACCTAAT

Downstream 100 bases:

>100_bases
CGGTGCGCCTGGCGACAAATTTCATAACAACAGTGCATCCAAAGCTGCGGTATCCTCGAAAATTGTGTTCAGACGCAAAA
CGGGTATCGTGCGCTAAATT

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MPEVTFLLTDPGEGLVEAEIVSLRVTEGDAVDVNQIVVEVETAKSLVELPSPFKGVVRKLLVAVGELVKVGSPIMLIDTD
ETPPAGLPKTGKMPVSDVRDGGHDTAKNTHPRDTGSSKDIHDAFGKEDGREIDFGRNVLVGYGPCEDNARVRGDDITGRP
SLDETLERPVAKPSVRKFAKELGVDLYGVKPTGIGGTITRRDVLNATSTQEEATTRVPVKGLRRQTAQNVTLSAFSVPHV
SVFVDVDVTRTIELVDRLKRDPLYEQVRISPLLILSRAVTWAVKRSPIVNSTWSEQEILLHKSVNLGIAVATDRGLVVPN
IKNAQCLSMLDLAKAIESLVDDARSSRIRPEDTLNGTITITNIGVFGVDSGTPILNVGESSIVFIGAIKPRPWVVNGEIS
VRRVATIGGSFDHRVMDGDTASRFLVNVASILEEPALLVE

Sequences:

>Translated_440_residues
MPEVTFLLTDPGEGLVEAEIVSLRVTEGDAVDVNQIVVEVETAKSLVELPSPFKGVVRKLLVAVGELVKVGSPIMLIDTD
ETPPAGLPKTGKMPVSDVRDGGHDTAKNTHPRDTGSSKDIHDAFGKEDGREIDFGRNVLVGYGPCEDNARVRGDDITGRP
SLDETLERPVAKPSVRKFAKELGVDLYGVKPTGIGGTITRRDVLNATSTQEEATTRVPVKGLRRQTAQNVTLSAFSVPHV
SVFVDVDVTRTIELVDRLKRDPLYEQVRISPLLILSRAVTWAVKRSPIVNSTWSEQEILLHKSVNLGIAVATDRGLVVPN
IKNAQCLSMLDLAKAIESLVDDARSSRIRPEDTLNGTITITNIGVFGVDSGTPILNVGESSIVFIGAIKPRPWVVNGEIS
VRRVATIGGSFDHRVMDGDTASRFLVNVASILEEPALLVE
>Mature_439_residues
PEVTFLLTDPGEGLVEAEIVSLRVTEGDAVDVNQIVVEVETAKSLVELPSPFKGVVRKLLVAVGELVKVGSPIMLIDTDE
TPPAGLPKTGKMPVSDVRDGGHDTAKNTHPRDTGSSKDIHDAFGKEDGREIDFGRNVLVGYGPCEDNARVRGDDITGRPS
LDETLERPVAKPSVRKFAKELGVDLYGVKPTGIGGTITRRDVLNATSTQEEATTRVPVKGLRRQTAQNVTLSAFSVPHVS
VFVDVDVTRTIELVDRLKRDPLYEQVRISPLLILSRAVTWAVKRSPIVNSTWSEQEILLHKSVNLGIAVATDRGLVVPNI
KNAQCLSMLDLAKAIESLVDDARSSRIRPEDTLNGTITITNIGVFGVDSGTPILNVGESSIVFIGAIKPRPWVVNGEISV
RRVATIGGSFDHRVMDGDTASRFLVNVASILEEPALLVE

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=474, Percent_Identity=26.3713080168776, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI19923748, Length=221, Percent_Identity=30.316742081448, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI203098816, Length=230, Percent_Identity=30, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI203098753, Length=229, Percent_Identity=30.1310043668122, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI31711992, Length=294, Percent_Identity=27.5510204081633, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI260898739, Length=154, Percent_Identity=31.1688311688312, Blast_Score=90, Evalue=5e-18,
Organism=Escherichia coli, GI1786946, Length=289, Percent_Identity=30.4498269896194, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1786305, Length=305, Percent_Identity=27.8688524590164, Blast_Score=111, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17537937, Length=445, Percent_Identity=25.6179775280899, Blast_Score=134, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI25146366, Length=430, Percent_Identity=26.9767441860465, Blast_Score=130, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI17538894, Length=241, Percent_Identity=28.6307053941909, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17560088, Length=445, Percent_Identity=24.7191011235955, Blast_Score=106, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6320352, Length=426, Percent_Identity=25.8215962441315, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6324258, Length=463, Percent_Identity=23.5421166306695, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24645909, Length=242, Percent_Identity=32.6446280991736, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI18859875, Length=302, Percent_Identity=28.476821192053, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24582497, Length=292, Percent_Identity=28.0821917808219, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI20129315, Length=292, Percent_Identity=28.0821917808219, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 47491; Mature: 47360

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEVTFLLTDPGEGLVEAEIVSLRVTEGDAVDVNQIVVEVETAKSLVELPSPFKGVVRKL
CCCEEEEEECCCCCCEEEEEEEEEECCCCCEEHHHEEEEEHHHHHHHHCCCCHHHHHHHH
LVAVGELVKVGSPIMLIDTDETPPAGLPKTGKMPVSDVRDGGHDTAKNTHPRDTGSSKDI
HHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCH
HDAFGKEDGREIDFGRNVLVGYGPCEDNARVRGDDITGRPSLDETLERPVAKPSVRKFAK
HHHHCCCCCCEECCCCCEEEEECCCCCCCEEECCCCCCCCCHHHHHHCCCCCHHHHHHHH
ELGVDLYGVKPTGIGGTITRRDVLNATSTQEEATTRVPVKGLRRQTAQNVTLSAFSVPHV
HHCCEEEECCCCCCCCCEEHHHHHCCCCCHHHHHCCCCHHHHHHHHHCCEEEEEECCCEE
SVFVDVDVTRTIELVDRLKRDPLYEQVRISPLLILSRAVTWAVKRSPIVNSTWSEQEILL
EEEEEECHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
HKSVNLGIAVATDRGLVVPNIKNAQCLSMLDLAKAIESLVDDARSSRIRPEDTLNGTITI
EECCCCEEEEECCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
TNIGVFGVDSGTPILNVGESSIVFIGAIKPRPWVVNGEISVRRVATIGGSFDHRVMDGDT
EEEEEEEECCCCCEEEECCCCEEEEEECCCCCEEEECCEEEEEEEEECCCCCCEEECCCH
ASRFLVNVASILEEPALLVE
HHHHHHHHHHHHCCCCEECC
>Mature Secondary Structure 
PEVTFLLTDPGEGLVEAEIVSLRVTEGDAVDVNQIVVEVETAKSLVELPSPFKGVVRKL
CCEEEEEECCCCCCEEEEEEEEEECCCCCEEHHHEEEEEHHHHHHHHCCCCHHHHHHHH
LVAVGELVKVGSPIMLIDTDETPPAGLPKTGKMPVSDVRDGGHDTAKNTHPRDTGSSKDI
HHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCH
HDAFGKEDGREIDFGRNVLVGYGPCEDNARVRGDDITGRPSLDETLERPVAKPSVRKFAK
HHHHCCCCCCEECCCCCEEEEECCCCCCCEEECCCCCCCCCHHHHHHCCCCCHHHHHHHH
ELGVDLYGVKPTGIGGTITRRDVLNATSTQEEATTRVPVKGLRRQTAQNVTLSAFSVPHV
HHCCEEEECCCCCCCCCEEHHHHHCCCCCHHHHHCCCCHHHHHHHHHCCEEEEEECCCEE
SVFVDVDVTRTIELVDRLKRDPLYEQVRISPLLILSRAVTWAVKRSPIVNSTWSEQEILL
EEEEEECHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
HKSVNLGIAVATDRGLVVPNIKNAQCLSMLDLAKAIESLVDDARSSRIRPEDTLNGTITI
EECCCCEEEEECCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
TNIGVFGVDSGTPILNVGESSIVFIGAIKPRPWVVNGEISVRRVATIGGSFDHRVMDGDT
EEEEEEEECCCCCEEEECCCCEEEEEECCCCCEEEECCEEEEEEEEECCCCCCEEECCCH
ASRFLVNVASILEEPALLVE
HHHHHHHHHHHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA