Click here to switch to the map view.

The map label for this gene is nhaA

Identifier: 28572936

GI number: 28572936

Start: 901522

End: 902679

Strand: Reverse

Name: nhaA

Synonym: TW795

Alternate gene names: 28572936

Gene position: 902679-901522 (Counterclockwise)

Preceding gene: 28572937

Following gene: 28572935

Centisome position: 97.49

GC content: 45.34

Gene sequence:

>1158_bases
ATGAGCATTATTAGATCTGAGCGATATTCGGCAATTTTTCTTCTTTGCTCGGCAGCGCTAGCAATTATATTTGCTAATGT
TCTTGACCCGGACACATGGCATGCCGTGCACAGCGCTGTAAGCGAATACCACATCTTTGGACTGATAACCCCCCATGACA
TAGTTGCAGATTTCCTTCTTGCAGTTTTCTTCTTTGCTGTTGCAATTGAGCTAAAACACGAGCTTGTAAAGGGCGAGCTG
AGTAGCTTTTCCAAAGCAATAATCCCTGGGGTGTGTGCAGCAGGTGGGATACTCGTTCCAATATCTATTTATTTGTCCGT
TGCAAGTGTTTTGCCAAACGGATGGCCTGTTCCGACGGCCACAGATGTTGCATTTTCTCTTGGCATTTTGGCTATTTTTG
GCTCTTCACTTCCTTCTAAGGTGCGAATATTCTTATTGGCTCTTGCTGTCTTGGATGATCTGGCCGGAATAGTGATAATT
GCCACGGCATTTAGCGTCTCGATAAGTTACTGGTGGATTATTGTTGCGTGCATTACAGTTGGGCTTTTTGGGTTTTGCAG
CTATAGGCTAGCCAGATGCAAGACCTCAAAGACATTTCTGATCATCCCTGCAATGTTGCTTTGTGCGCTCGCGGCATGGG
TTTCTGTTTACCAGTCCGGTATTCATGCGACTATCGCTGGCGTTATGCTTGGCATCATGCTCAATCGTAAACAGGGTGCG
GCTATTGAACACGCTCTTGAGCCGTATATAAACGGGATTATTCTGCCCGCGTTTGCTTTTCTTGCTGCAATGGTTCGGGT
ACCACATTTACCTCTGGATGAGATAAGTCCGGCGCTATGGGGAATTCTGCTTGGTCTGTTATTTGGCAAACTCCTCGGAA
TAAGCGTTTTTGGCATAATAGCCCTGAAGTTCTTCAGAAAAAAGAGTATTTCATTCTTCAACTTGCTTGTGGTAAGTGCT
CTTGGCGGAATTGGATTTACGGTGTCTTTGCTCATGAACGAGCTAGCTTTTTTGGGAACCCCTGTTCATGAACAGGGGGT
TATTGCCGTTTTGATTGGTTCGCTTTTGTCTGCCATATTGGCAATTATTCTCATGAGGTGTTATAAAGGCCGTAAATCTA
AGTCATTGCCAGGTAGAAAGGGCCGGGTCAGCCATTGA

Upstream 100 bases:

>100_bases
GTTCGCCCGTATGTGCCTCTAGGTACCAGGGACAGCGCGGCCCAACCGCGAGTCTTTCGCATCAGCATTTTTATCGCGCA
CGTTTTACAGAAATAGGATT

Downstream 100 bases:

>100_bases
AGATTGTTCCTCCGCGAGGCATGCAGGACTTTCTCCCGCATGAGAAAGAACATCGCGATCGTATTACTGAGGTCATATAT
AAAAGCTACATATCGCACGG

Product: sodium/proton antiporter

Products: Na (I) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: Sodium/proton antiporter nhaA

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MSIIRSERYSAIFLLCSAALAIIFANVLDPDTWHAVHSAVSEYHIFGLITPHDIVADFLLAVFFFAVAIELKHELVKGEL
SSFSKAIIPGVCAAGGILVPISIYLSVASVLPNGWPVPTATDVAFSLGILAIFGSSLPSKVRIFLLALAVLDDLAGIVII
ATAFSVSISYWWIIVACITVGLFGFCSYRLARCKTSKTFLIIPAMLLCALAAWVSVYQSGIHATIAGVMLGIMLNRKQGA
AIEHALEPYINGIILPAFAFLAAMVRVPHLPLDEISPALWGILLGLLFGKLLGISVFGIIALKFFRKKSISFFNLLVVSA
LGGIGFTVSLLMNELAFLGTPVHEQGVIAVLIGSLLSAILAIILMRCYKGRKSKSLPGRKGRVSH

Sequences:

>Translated_385_residues
MSIIRSERYSAIFLLCSAALAIIFANVLDPDTWHAVHSAVSEYHIFGLITPHDIVADFLLAVFFFAVAIELKHELVKGEL
SSFSKAIIPGVCAAGGILVPISIYLSVASVLPNGWPVPTATDVAFSLGILAIFGSSLPSKVRIFLLALAVLDDLAGIVII
ATAFSVSISYWWIIVACITVGLFGFCSYRLARCKTSKTFLIIPAMLLCALAAWVSVYQSGIHATIAGVMLGIMLNRKQGA
AIEHALEPYINGIILPAFAFLAAMVRVPHLPLDEISPALWGILLGLLFGKLLGISVFGIIALKFFRKKSISFFNLLVVSA
LGGIGFTVSLLMNELAFLGTPVHEQGVIAVLIGSLLSAILAIILMRCYKGRKSKSLPGRKGRVSH
>Mature_384_residues
SIIRSERYSAIFLLCSAALAIIFANVLDPDTWHAVHSAVSEYHIFGLITPHDIVADFLLAVFFFAVAIELKHELVKGELS
SFSKAIIPGVCAAGGILVPISIYLSVASVLPNGWPVPTATDVAFSLGILAIFGSSLPSKVRIFLLALAVLDDLAGIVIIA
TAFSVSISYWWIIVACITVGLFGFCSYRLARCKTSKTFLIIPAMLLCALAAWVSVYQSGIHATIAGVMLGIMLNRKQGAA
IEHALEPYINGIILPAFAFLAAMVRVPHLPLDEISPALWGILLGLLFGKLLGISVFGIIALKFFRKKSISFFNLLVVSAL
GGIGFTVSLLMNELAFLGTPVHEQGVIAVLIGSLLSAILAIILMRCYKGRKSKSLPGRKGRVSH

Specific function: Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons

COG id: COG3004

COG function: function code P; Na+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaA Na(+)/H(+) (TC 2.A.33) antiporter family

Homologues:

Organism=Escherichia coli, GI1786201, Length=327, Percent_Identity=36.697247706422, Blast_Score=172, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NHAA_TROW8 (Q83N58)

Other databases:

- EMBL:   BX251412
- RefSeq:   NP_789716.1
- HSSP:   P13738
- ProteinModelPortal:   Q83N58
- SMR:   Q83N58
- STRING:   Q83N58
- GeneID:   1064995
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW795
- eggNOG:   COG3004
- HOGENOM:   HBG665619
- OMA:   SERYSAI
- PhylomeDB:   Q83N58
- ProtClustDB:   CLSK230290
- BioCyc:   TWHI218496:TW0761-MONOMER
- HAMAP:   MF_01844
- InterPro:   IPR023171
- InterPro:   IPR004670
- TIGRFAMs:   TIGR00773

Pfam domain/function: PF06965 Na_H_antiport_1

EC number: NA

Molecular weight: Translated: 41235; Mature: 41104

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xc75751c)-; HASH(0xd662490)-; HASH(0xd66b580)-; HASH(0xc2d09ec)-; HASH(0xd6852dc)-; HASH(0xd658418)-; HASH(0xc2d0848)-; HASH(0xd658388)-; HASH(0xd6812a8)-; HASH(0xd66261c)-; HASH(0xce161ac)-;

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIIRSERYSAIFLLCSAALAIIFANVLDPDTWHAVHSAVSEYHIFGLITPHDIVADFLL
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEECCHHHHHHHHHH
AVFFFAVAIELKHELVKGELSSFSKAIIPGVCAAGGILVPISIYLSVASVLPNGWPVPTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCH
TDVAFSLGILAIFGSSLPSKVRIFLLALAVLDDLAGIVIIATAFSVSISYWWIIVACITV
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLFGFCSYRLARCKTSKTFLIIPAMLLCALAAWVSVYQSGIHATIAGVMLGIMLNRKQGA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
AIEHALEPYINGIILPAFAFLAAMVRVPHLPLDEISPALWGILLGLLFGKLLGISVFGII
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALKFFRKKSISFFNLLVVSALGGIGFTVSLLMNELAFLGTPVHEQGVIAVLIGSLLSAIL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
AIILMRCYKGRKSKSLPGRKGRVSH
HHHHHHHHCCCCCCCCCCCCCCCCH
>Mature Secondary Structure 
SIIRSERYSAIFLLCSAALAIIFANVLDPDTWHAVHSAVSEYHIFGLITPHDIVADFLL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEECCHHHHHHHHHH
AVFFFAVAIELKHELVKGELSSFSKAIIPGVCAAGGILVPISIYLSVASVLPNGWPVPTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCH
TDVAFSLGILAIFGSSLPSKVRIFLLALAVLDDLAGIVIIATAFSVSISYWWIIVACITV
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLFGFCSYRLARCKTSKTFLIIPAMLLCALAAWVSVYQSGIHATIAGVMLGIMLNRKQGA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
AIEHALEPYINGIILPAFAFLAAMVRVPHLPLDEISPALWGILLGLLFGKLLGISVFGII
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALKFFRKKSISFFNLLVVSALGGIGFTVSLLMNELAFLGTPVHEQGVIAVLIGSLLSAIL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
AIILMRCYKGRKSKSLPGRKGRVSH
HHHHHHHHCCCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + 2 Proton [Periplasm] = Na (I) [Periplasm] + 2 Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12606174