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The map label for this gene is hisS

Identifier: 28572935

GI number: 28572935

Start: 900245

End: 901525

Strand: Reverse

Name: hisS

Synonym: TW794

Alternate gene names: 28572935

Gene position: 901525-900245 (Counterclockwise)

Preceding gene: 28572936

Following gene: 28572934

Centisome position: 97.36

GC content: 45.36

Gene sequence:

>1281_bases
TTGAAGATTGTTCCTCCGCGAGGCATGCAGGACTTTCTCCCGCATGAGAAAGAACATCGCGATCGTATTACTGAGGTCAT
ATATAAAAGCTACATATCGCACGGCTTCAACCCAATCGAGACACCCTCTCTCGAAAATATTGAACGTTTGGCCTGCGGTG
TCGGTCAGGAAAATGAAAAACTAACCTACAAGATCATTCGGCGTGGTCTTACCGGCGCACAAACTGTGCAGCACCCGGAT
GAGCTTATTGATTTAGGGTTGCGATTTGATTTGACTATTCCTCTTGTTAGGTTCTGGAATACAAACCGGGCGCGTCTTCC
CAAGATATTTCGCTCGCTTCAGATTGGCCATGTCTGGCGCGCTGAAAAGCCACAGAAGGGCCGCCGCCGGCAATTTATTC
AGTGCGATATAGACATAATTGGGCAGCCAGAGATATTGGCCGAGATTGAGTTACTTGTGGCAACCCTTTCTACACTCGAA
CAGCTTGGCATACGAACACCGAAACTTCATATAAATGACAGAAGAATTCTTTTTTCTATGCTCAATAACCTTGGCGTTCC
GCACAGTTGTCATGTATATGTTTCTATTGTTCTTGACAAATTGCGTAAAATTGGCCTCGATCTTGTCAAACAAGAGCTTT
GTGAATTCCCTGCACTTGTTGCGTATCTGGCTTCTTCTGTCAATTCAAATACCGGTAGTACGGATCTTTGTTTTGATGTA
TCAAGTACAAAGGATATTACACATATCAGACGTACGATTCAGTCAGCCCTCCCGCACGGTTGTAAATTTGACTGTGAGGA
TTTGTGCAGAATAATTGCTTCGGTCAACGAGTTTACACAAACTGGTGTATTTTTTGACCCGCTCCTTGTACGAGGCATGG
GATATTACACGGGTCCTATTTTTGAGATATTGCATGATGATTATTCCATTGCTGGAGGTGGAAGATACGATGGCCTGGTC
GAACGCCTCGGTGGGTTACCAACTCCGGCATGCGGGTTTTCAATAGGTTTTGAGCGTGTACTGGGCCTGATCAAGGAATC
AGTGTCTTTGGATCCGAAAAAAATGATTCTTTTGTATGACCCCAAAGTTGATCCGAACCTGGTTGTATCCGTCAAATTGG
AGTTCATTTCGAAAGGTTTTATTGTCCGTCCAGAGCTTGCATCCCGCAGTCGACGTAATCAAATCGAGCTTGCCAAACGA
GAGGGCTTTGGTGCGTTTCTCTACCTTGATCCCCTATCGCCACCCGATGGCCTTTTAGCAAAAGTAAAGCCGATCTTGTG
A

Upstream 100 bases:

>100_bases
TTGGTTCGCTTTTGTCTGCCATATTGGCAATTATTCTCATGAGGTGTTATAAAGGCCGTAAATCTAAGTCATTGCCAGGT
AGAAAGGGCCGGGTCAGCCA

Downstream 100 bases:

>100_bases
TTATGATTTTGTGCAATCGCACGGCGCTGGGGGCTATAGATGACACAAAGGGTATGCACGACGCGCCTCATTCGGGAGGT
TCATGTCCACTTGCGCTGCT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MKIVPPRGMQDFLPHEKEHRDRITEVIYKSYISHGFNPIETPSLENIERLACGVGQENEKLTYKIIRRGLTGAQTVQHPD
ELIDLGLRFDLTIPLVRFWNTNRARLPKIFRSLQIGHVWRAEKPQKGRRRQFIQCDIDIIGQPEILAEIELLVATLSTLE
QLGIRTPKLHINDRRILFSMLNNLGVPHSCHVYVSIVLDKLRKIGLDLVKQELCEFPALVAYLASSVNSNTGSTDLCFDV
SSTKDITHIRRTIQSALPHGCKFDCEDLCRIIASVNEFTQTGVFFDPLLVRGMGYYTGPIFEILHDDYSIAGGGRYDGLV
ERLGGLPTPACGFSIGFERVLGLIKESVSLDPKKMILLYDPKVDPNLVVSVKLEFISKGFIVRPELASRSRRNQIELAKR
EGFGAFLYLDPLSPPDGLLAKVKPIL

Sequences:

>Translated_426_residues
MKIVPPRGMQDFLPHEKEHRDRITEVIYKSYISHGFNPIETPSLENIERLACGVGQENEKLTYKIIRRGLTGAQTVQHPD
ELIDLGLRFDLTIPLVRFWNTNRARLPKIFRSLQIGHVWRAEKPQKGRRRQFIQCDIDIIGQPEILAEIELLVATLSTLE
QLGIRTPKLHINDRRILFSMLNNLGVPHSCHVYVSIVLDKLRKIGLDLVKQELCEFPALVAYLASSVNSNTGSTDLCFDV
SSTKDITHIRRTIQSALPHGCKFDCEDLCRIIASVNEFTQTGVFFDPLLVRGMGYYTGPIFEILHDDYSIAGGGRYDGLV
ERLGGLPTPACGFSIGFERVLGLIKESVSLDPKKMILLYDPKVDPNLVVSVKLEFISKGFIVRPELASRSRRNQIELAKR
EGFGAFLYLDPLSPPDGLLAKVKPIL
>Mature_426_residues
MKIVPPRGMQDFLPHEKEHRDRITEVIYKSYISHGFNPIETPSLENIERLACGVGQENEKLTYKIIRRGLTGAQTVQHPD
ELIDLGLRFDLTIPLVRFWNTNRARLPKIFRSLQIGHVWRAEKPQKGRRRQFIQCDIDIIGQPEILAEIELLVATLSTLE
QLGIRTPKLHINDRRILFSMLNNLGVPHSCHVYVSIVLDKLRKIGLDLVKQELCEFPALVAYLASSVNSNTGSTDLCFDV
SSTKDITHIRRTIQSALPHGCKFDCEDLCRIIASVNEFTQTGVFFDPLLVRGMGYYTGPIFEILHDDYSIAGGGRYDGLV
ERLGGLPTPACGFSIGFERVLGLIKESVSLDPKKMILLYDPKVDPNLVVSVKLEFISKGFIVRPELASRSRRNQIELAKR
EGFGAFLYLDPLSPPDGLLAKVKPIL

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI15029520, Length=373, Percent_Identity=28.1501340482574, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI6996014, Length=362, Percent_Identity=30.1104972375691, Blast_Score=125, Evalue=9e-29,
Organism=Escherichia coli, GI1788861, Length=375, Percent_Identity=28, Blast_Score=88, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI71993693, Length=363, Percent_Identity=28.6501377410468, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71993686, Length=363, Percent_Identity=28.6501377410468, Blast_Score=126, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6325290, Length=384, Percent_Identity=28.3854166666667, Blast_Score=116, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24643061, Length=374, Percent_Identity=27.807486631016, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24643059, Length=393, Percent_Identity=26.7175572519084, Blast_Score=105, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_TROW8 (Q83H72)

Other databases:

- EMBL:   BX251412
- RefSeq:   NP_789715.1
- ProteinModelPortal:   Q83H72
- SMR:   Q83H72
- STRING:   Q83H72
- GeneID:   1064994
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW794
- eggNOG:   COG0124
- HOGENOM:   HBG616575
- OMA:   ALDIARC
- PhylomeDB:   Q83H72
- ProtClustDB:   PRK00037
- BioCyc:   TWHI218496:TW0760-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR015807
- InterPro:   IPR004516
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF00587 tRNA-synt_2b

EC number: =6.1.1.21

Molecular weight: Translated: 48040; Mature: 48040

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVPPRGMQDFLPHEKEHRDRITEVIYKSYISHGFNPIETPSLENIERLACGVGQENEK
CCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCH
LTYKIIRRGLTGAQTVQHPDELIDLGLRFDLTIPLVRFWNTNRARLPKIFRSLQIGHVWR
HHHHHHHCCCCCCHHHCCHHHHHHHCCCEEEEEHHHHHCCCCHHHHHHHHHHHHHCCCCC
AEKPQKGRRRQFIQCDIDIIGQPEILAEIELLVATLSTLEQLGIRTPKLHINDRRILFSM
CCCCCCCHHCCEEEECCEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHH
LNNLGVPHSCHVYVSIVLDKLRKIGLDLVKQELCEFPALVAYLASSVNSNTGSTDLCFDV
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEC
SSTKDITHIRRTIQSALPHGCKFDCEDLCRIIASVNEFTQTGVFFDPLLVRGMGYYTGPI
CCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHH
FEILHDDYSIAGGGRYDGLVERLGGLPTPACGFSIGFERVLGLIKESVSLDPKKMILLYD
HHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEC
PKVDPNLVVSVKLEFISKGFIVRPELASRSRRNQIELAKREGFGAFLYLDPLSPPDGLLA
CCCCCCEEEEEEEEHHHCCCEECCHHHHHHHCCHHHHHHHCCCEEEEEECCCCCCCCHHH
KVKPIL
HHCCCC
>Mature Secondary Structure
MKIVPPRGMQDFLPHEKEHRDRITEVIYKSYISHGFNPIETPSLENIERLACGVGQENEK
CCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCH
LTYKIIRRGLTGAQTVQHPDELIDLGLRFDLTIPLVRFWNTNRARLPKIFRSLQIGHVWR
HHHHHHHCCCCCCHHHCCHHHHHHHCCCEEEEEHHHHHCCCCHHHHHHHHHHHHHCCCCC
AEKPQKGRRRQFIQCDIDIIGQPEILAEIELLVATLSTLEQLGIRTPKLHINDRRILFSM
CCCCCCCHHCCEEEECCEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHH
LNNLGVPHSCHVYVSIVLDKLRKIGLDLVKQELCEFPALVAYLASSVNSNTGSTDLCFDV
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEC
SSTKDITHIRRTIQSALPHGCKFDCEDLCRIIASVNEFTQTGVFFDPLLVRGMGYYTGPI
CCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHH
FEILHDDYSIAGGGRYDGLVERLGGLPTPACGFSIGFERVLGLIKESVSLDPKKMILLYD
HHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEC
PKVDPNLVVSVKLEFISKGFIVRPELASRSRRNQIELAKREGFGAFLYLDPLSPPDGLLA
CCCCCCEEEEEEEEHHHCCCEECCHHHHHHHCCHHHHHHHCCCEEEEEECCCCCCCCHHH
KVKPIL
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12606174