Click here to switch to the map view.

The map label for this gene is topA [H]

Identifier: 28572856

GI number: 28572856

Start: 808491

End: 811016

Strand: Reverse

Name: topA [H]

Synonym: TW715

Alternate gene names: 28572856

Gene position: 811016-808491 (Counterclockwise)

Preceding gene: 28572857

Following gene: 28572855

Centisome position: 87.59

GC content: 48.81

Gene sequence:

>2526_bases
ATGAAAAAACTTGTTATAGTCGAGTCGCCAACCAAGGTATCGTCGGTTGGTCGTTACCTTGGTGACGGTTATGTTGTCAT
GTCGTCAGTAGGGCATATTCGTGATTTGCCCGCCCCTAGTGGGCTTACCCCAGATCTTAAGAGGTCCGCTCATGCCCAGT
TTGCAGTTAATATTGAAAACGATTTTGAGCCCTACTATGTCGTCCTGGAGAATAAAAAGAAGGTAGTTGAGGAGCTACAG
TCGGCCCTGAATAATTCGGACGAGCTCCTTATAGCGACAGACGGTGATCGGGAAGGTGAGGCGATAGCGTGGCATTTGGT
CCAGGTTCTGAAGCCTACTGTGCCGGTGAGGCGATTGGTATTTCACGAGATTACTAGGGAAGCCATCGCGTATGCACTAC
AACATCCGAGGCCGATTGATGAAAACCTTGTTGACGCGCAGGAGGCTCGGAGAATTCTGGACAGACTCTATGGTTACGAG
CTATCCCCGGTGCTCTGGAAAAAGGTAAGGCCCGGGTTGTCTGCCGGTAGAGTTCAGTCTCCCGCTACCCGACTTCTGGT
GGATCGTGAGCGTGAACGGATCAACTTCAAGTCGGCTGAATATCAAGCGGTAACGGCTGACTTTCAGGTGGATTCCGACG
TATTCTCAGCTTCTTTGACGCTTTTCGGTGAGAGGAAAATCGCCACCGGGCGTGACTTTGACAATTTGGCAAATCTCAAA
ACAGATGCTTTAGTACTAGATAAAAATCTAACCGATCGCATACTCGAAGAGCTCAAAAGTAAGGGTGCTTTGGATGTTGA
AGCAATCGATTCAAAGCCGTATAGCAGAAACCCTTATGCTCCGTTTATCACTTCCACCCTGCAGCAGGATGCCGCACATA
AACTGGGGTTCTCAGCTAAACGAACAATGCGCACTGCCCAACAATTGTATGAAAAAGGGTTTATCACGTACATGAGAACG
GACTCACCACGTCTGTCTCTACAAGCGATTTCCGCCGCGAGAGCTCAGGTTGAGCTTCTCTACGGAAGGGAGTTTCTACC
GGATTCCCCAAGAACCTACGGGGCAAAGGCAAAGAACGCCCAAGAAGCGCATGAGGCGATAAGGCCTACTGGCGAGGTGT
TTGAAAGGCCTGAGTCGATAGAATCTGTTTTATCGGTTGACTGCATGAGGCTTTACGACCTTATATGGAGGCGAACGATT
GCTTCCCAGATGGTATCTGCTAGGGGTGAAACTGTCACCCTGACCCTCTCTGCCAGAATTCCGGAGCGATCTATTTTTGT
CCTGTCCGGAACTGTTCTGCATGAAAAGGGTTTCTTGAAAGTGTATGAAGGGTTGCAGGATCTCAACTCTAAGCTATTAC
CAAATTTACAGGTCGGCGATCAGGTTACCCTTGTTGATGTTCGTGCGGTCGGGCACACAACCCTTCCGCCCCCACGATAC
ACCGAGGCTAGCCTGGTAAAGGTCCTAGAGGAGTTGGGGCTGGGTCGCCCATCAACATATGCGCTTATTTTGAGAACAAT
TCAGGAGCGCGGCTATGCTCATCTCAGGAACAGGATGCTTGTTCCTGATTGGCTTGCTTTCTCTGTTGTCAATCTTCTTG
AGAACTTCTTCTCTCGACTAATTGATTACGATTTTACTGCGAACCTCGAGGATCAGCTTGATGAGATCGCACTCGGCTCA
CAGAATAGATCGGACTGGCTTCGTAAGTTTTACTTTGGTGATGACAATCTTCCTGGATTGAAGGAGATAATTTCAGGCGC
AAAAGACATAGATGCACGAGCAATCAATTCGATCCCAATGGTCGGGGCGACTCTTCGGGTAGGGAGATATGGCCCGTATC
TTGAAGTTGGGGATAAGAGATTCAGCATTCCGCATGATGTATACCCCGATGCCTTGACTCCCGAAAAAATACATGAGCTG
GTTAATCAGCCAGAATCCCCGGTAAGAGAACTCGGCGCTCACCCGGAGACCGGAAAATTAGTTGTCTTAAAGTCCGGTCG
CTTTGGCCCATATATCGAGGAAGTTTCGGATGAGAAAAATGTCAGACGATCAAGTCTGTTCAAGAACATGGATCCAGAGG
AAGTTAACTTAGATGTGGCTGTTAAGCTGCTGGAAATACCCAGAGTCATTGGGGTCAACCAAGAGAACGGAGAAAAGGTT
ATTGCTTGTAACGGTCGCTTTGGGCCATATATAAAATGTGGTAAGGAGACACGTCCCCTAGACAGTGAAGATGACATTCT
CTCGGTGACGCTTGACGAGGCATTAGCCATCCTGTCCGCACCGAAAGTTAGGCGGTCACGTGTTCTTATGGAATTTCCAA
CGGATGATCCGGTCACAAGGCGCGCAGTGCAAATCAAGTCCGGTCGCTTTGGGCCATATGTAACAGATGGCGTTACAAAT
GCAACTTTGGACAATACATCGTGGGAGGATGTGACGTTTGACGAAGCTGTGTCCCTGCTCGCGGAAAAGCGTCAACGGGT
TGATGGTGATCTTGATCAAGAAACTGCAGAAAAGATCCATGTGTAG

Upstream 100 bases:

>100_bases
AATACGAGCAACCGCAAAAGCCGGCGCACCGCCTTCTTAAAGACTCTGAGTATCCTTGTCATTTGCGAATCTGCTTGAGA
GTAATATTAGATAGGTTTTT

Downstream 100 bases:

>100_bases
GATTTTACATATCGGATACACCGTCAGTTATTCTCGTATGGCCTCAAAGAGGGTTTTGCTTGTTTGTAACCTTTGAGGGT
ATTGATGGAAGCGGGAAGAC

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 841; Mature: 841

Protein sequence:

>841_residues
MKKLVIVESPTKVSSVGRYLGDGYVVMSSVGHIRDLPAPSGLTPDLKRSAHAQFAVNIENDFEPYYVVLENKKKVVEELQ
SALNNSDELLIATDGDREGEAIAWHLVQVLKPTVPVRRLVFHEITREAIAYALQHPRPIDENLVDAQEARRILDRLYGYE
LSPVLWKKVRPGLSAGRVQSPATRLLVDRERERINFKSAEYQAVTADFQVDSDVFSASLTLFGERKIATGRDFDNLANLK
TDALVLDKNLTDRILEELKSKGALDVEAIDSKPYSRNPYAPFITSTLQQDAAHKLGFSAKRTMRTAQQLYEKGFITYMRT
DSPRLSLQAISAARAQVELLYGREFLPDSPRTYGAKAKNAQEAHEAIRPTGEVFERPESIESVLSVDCMRLYDLIWRRTI
ASQMVSARGETVTLTLSARIPERSIFVLSGTVLHEKGFLKVYEGLQDLNSKLLPNLQVGDQVTLVDVRAVGHTTLPPPRY
TEASLVKVLEELGLGRPSTYALILRTIQERGYAHLRNRMLVPDWLAFSVVNLLENFFSRLIDYDFTANLEDQLDEIALGS
QNRSDWLRKFYFGDDNLPGLKEIISGAKDIDARAINSIPMVGATLRVGRYGPYLEVGDKRFSIPHDVYPDALTPEKIHEL
VNQPESPVRELGAHPETGKLVVLKSGRFGPYIEEVSDEKNVRRSSLFKNMDPEEVNLDVAVKLLEIPRVIGVNQENGEKV
IACNGRFGPYIKCGKETRPLDSEDDILSVTLDEALAILSAPKVRRSRVLMEFPTDDPVTRRAVQIKSGRFGPYVTDGVTN
ATLDNTSWEDVTFDEAVSLLAEKRQRVDGDLDQETAEKIHV

Sequences:

>Translated_841_residues
MKKLVIVESPTKVSSVGRYLGDGYVVMSSVGHIRDLPAPSGLTPDLKRSAHAQFAVNIENDFEPYYVVLENKKKVVEELQ
SALNNSDELLIATDGDREGEAIAWHLVQVLKPTVPVRRLVFHEITREAIAYALQHPRPIDENLVDAQEARRILDRLYGYE
LSPVLWKKVRPGLSAGRVQSPATRLLVDRERERINFKSAEYQAVTADFQVDSDVFSASLTLFGERKIATGRDFDNLANLK
TDALVLDKNLTDRILEELKSKGALDVEAIDSKPYSRNPYAPFITSTLQQDAAHKLGFSAKRTMRTAQQLYEKGFITYMRT
DSPRLSLQAISAARAQVELLYGREFLPDSPRTYGAKAKNAQEAHEAIRPTGEVFERPESIESVLSVDCMRLYDLIWRRTI
ASQMVSARGETVTLTLSARIPERSIFVLSGTVLHEKGFLKVYEGLQDLNSKLLPNLQVGDQVTLVDVRAVGHTTLPPPRY
TEASLVKVLEELGLGRPSTYALILRTIQERGYAHLRNRMLVPDWLAFSVVNLLENFFSRLIDYDFTANLEDQLDEIALGS
QNRSDWLRKFYFGDDNLPGLKEIISGAKDIDARAINSIPMVGATLRVGRYGPYLEVGDKRFSIPHDVYPDALTPEKIHEL
VNQPESPVRELGAHPETGKLVVLKSGRFGPYIEEVSDEKNVRRSSLFKNMDPEEVNLDVAVKLLEIPRVIGVNQENGEKV
IACNGRFGPYIKCGKETRPLDSEDDILSVTLDEALAILSAPKVRRSRVLMEFPTDDPVTRRAVQIKSGRFGPYVTDGVTN
ATLDNTSWEDVTFDEAVSLLAEKRQRVDGDLDQETAEKIHV
>Mature_841_residues
MKKLVIVESPTKVSSVGRYLGDGYVVMSSVGHIRDLPAPSGLTPDLKRSAHAQFAVNIENDFEPYYVVLENKKKVVEELQ
SALNNSDELLIATDGDREGEAIAWHLVQVLKPTVPVRRLVFHEITREAIAYALQHPRPIDENLVDAQEARRILDRLYGYE
LSPVLWKKVRPGLSAGRVQSPATRLLVDRERERINFKSAEYQAVTADFQVDSDVFSASLTLFGERKIATGRDFDNLANLK
TDALVLDKNLTDRILEELKSKGALDVEAIDSKPYSRNPYAPFITSTLQQDAAHKLGFSAKRTMRTAQQLYEKGFITYMRT
DSPRLSLQAISAARAQVELLYGREFLPDSPRTYGAKAKNAQEAHEAIRPTGEVFERPESIESVLSVDCMRLYDLIWRRTI
ASQMVSARGETVTLTLSARIPERSIFVLSGTVLHEKGFLKVYEGLQDLNSKLLPNLQVGDQVTLVDVRAVGHTTLPPPRY
TEASLVKVLEELGLGRPSTYALILRTIQERGYAHLRNRMLVPDWLAFSVVNLLENFFSRLIDYDFTANLEDQLDEIALGS
QNRSDWLRKFYFGDDNLPGLKEIISGAKDIDARAINSIPMVGATLRVGRYGPYLEVGDKRFSIPHDVYPDALTPEKIHEL
VNQPESPVRELGAHPETGKLVVLKSGRFGPYIEEVSDEKNVRRSSLFKNMDPEEVNLDVAVKLLEIPRVIGVNQENGEKV
IACNGRFGPYIKCGKETRPLDSEDDILSVTLDEALAILSAPKVRRSRVLMEFPTDDPVTRRAVQIKSGRFGPYVTDGVTN
ATLDNTSWEDVTFDEAVSLLAEKRQRVDGDLDQETAEKIHV

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=540, Percent_Identity=26.1111111111111, Blast_Score=118, Evalue=2e-26,
Organism=Homo sapiens, GI4507635, Length=589, Percent_Identity=24.4482173174873, Blast_Score=114, Evalue=5e-25,
Organism=Escherichia coli, GI1787529, Length=587, Percent_Identity=41.7376490630324, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17555378, Length=527, Percent_Identity=26.3757115749526, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32563869, Length=598, Percent_Identity=25.752508361204, Blast_Score=115, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6323263, Length=603, Percent_Identity=25.7048092868988, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24585251, Length=518, Percent_Identity=27.2200772200772, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24640096, Length=591, Percent_Identity=24.8730964467005, Blast_Score=100, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 94340; Mature: 94340

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLVIVESPTKVSSVGRYLGDGYVVMSSVGHIRDLPAPSGLTPDLKRSAHAQFAVNIEN
CCCEEEEECCCHHHHHHHHHCCCEEEECCCCHHHCCCCCCCCCHHHHHCCCCEEEEEECC
DFEPYYVVLENKKKVVEELQSALNNSDELLIATDGDREGEAIAWHLVQVLKPTVPVRRLV
CCCCEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHH
FHEITREAIAYALQHPRPIDENLVDAQEARRILDRLYGYELSPVLWKKVRPGLSAGRVQS
HHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCC
PATRLLVDRERERINFKSAEYQAVTADFQVDSDVFSASLTLFGERKIATGRDFDNLANLK
CHHHHHHHHHHHHCCCCCCCEEEEEECCEECHHHHHHHHHEEECCCCCCCCCHHHHHCCC
TDALVLDKNLTDRILEELKSKGALDVEAIDSKPYSRNPYAPFITSTLQQDAAHKLGFSAK
CCEEEECCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHH
RTMRTAQQLYEKGFITYMRTDSPRLSLQAISAARAQVELLYGREFLPDSPRTYGAKAKNA
HHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCH
QEAHEAIRPTGEVFERPESIESVLSVDCMRLYDLIWRRTIASQMVSARGETVTLTLSARI
HHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECC
PERSIFVLSGTVLHEKGFLKVYEGLQDLNSKLLPNLQVGDQVTLVDVRAVGHTTLPPPRY
CCCEEEEEECEEEECCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCC
TEASLVKVLEELGLGRPSTYALILRTIQERGYAHLRNRMLVPDWLAFSVVNLLENFFSRL
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
IDYDFTANLEDQLDEIALGSQNRSDWLRKFYFGDDNLPGLKEIISGAKDIDARAINSIPM
HCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHHCCCCCCCHHHHCCCCC
VGATLRVGRYGPYLEVGDKRFSIPHDVYPDALTPEKIHELVNQPESPVRELGAHPETGKL
CCCEEEECCCCCCEEECCCEECCCCCCCCCCCCHHHHHHHHCCCCHHHHHHCCCCCCCEE
VVLKSGRFGPYIEEVSDEKNVRRSSLFKNMDPEEVNLDVAVKLLEIPRVIGVNQENGEKV
EEEECCCCCCHHHHHCCHHHHHHHHHHCCCCHHHCCHHHHHHHHHCCHHHCCCCCCCCEE
IACNGRFGPYIKCGKETRPLDSEDDILSVTLDEALAILSAPKVRRSRVLMEFPTDDPVTR
EEECCCCCCCEECCCCCCCCCCCCCEEEEEHHHHHHHHCCCCHHHCEEEEECCCCCCHHH
RAVQIKSGRFGPYVTDGVTNATLDNTSWEDVTFDEAVSLLAEKRQRVDGDLDQETAEKIH
HEEEEECCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCC
V
C
>Mature Secondary Structure
MKKLVIVESPTKVSSVGRYLGDGYVVMSSVGHIRDLPAPSGLTPDLKRSAHAQFAVNIEN
CCCEEEEECCCHHHHHHHHHCCCEEEECCCCHHHCCCCCCCCCHHHHHCCCCEEEEEECC
DFEPYYVVLENKKKVVEELQSALNNSDELLIATDGDREGEAIAWHLVQVLKPTVPVRRLV
CCCCEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHH
FHEITREAIAYALQHPRPIDENLVDAQEARRILDRLYGYELSPVLWKKVRPGLSAGRVQS
HHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCC
PATRLLVDRERERINFKSAEYQAVTADFQVDSDVFSASLTLFGERKIATGRDFDNLANLK
CHHHHHHHHHHHHCCCCCCCEEEEEECCEECHHHHHHHHHEEECCCCCCCCCHHHHHCCC
TDALVLDKNLTDRILEELKSKGALDVEAIDSKPYSRNPYAPFITSTLQQDAAHKLGFSAK
CCEEEECCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHH
RTMRTAQQLYEKGFITYMRTDSPRLSLQAISAARAQVELLYGREFLPDSPRTYGAKAKNA
HHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCH
QEAHEAIRPTGEVFERPESIESVLSVDCMRLYDLIWRRTIASQMVSARGETVTLTLSARI
HHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECC
PERSIFVLSGTVLHEKGFLKVYEGLQDLNSKLLPNLQVGDQVTLVDVRAVGHTTLPPPRY
CCCEEEEEECEEEECCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCC
TEASLVKVLEELGLGRPSTYALILRTIQERGYAHLRNRMLVPDWLAFSVVNLLENFFSRL
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
IDYDFTANLEDQLDEIALGSQNRSDWLRKFYFGDDNLPGLKEIISGAKDIDARAINSIPM
HCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHHCCCCCCCHHHHCCCCC
VGATLRVGRYGPYLEVGDKRFSIPHDVYPDALTPEKIHELVNQPESPVRELGAHPETGKL
CCCEEEECCCCCCEEECCCEECCCCCCCCCCCCHHHHHHHHCCCCHHHHHHCCCCCCCEE
VVLKSGRFGPYIEEVSDEKNVRRSSLFKNMDPEEVNLDVAVKLLEIPRVIGVNQENGEKV
EEEECCCCCCHHHHHCCHHHHHHHHHHCCCCHHHCCHHHHHHHHHCCHHHCCCCCCCCEE
IACNGRFGPYIKCGKETRPLDSEDDILSVTLDEALAILSAPKVRRSRVLMEFPTDDPVTR
EEECCCCCCCEECCCCCCCCCCCCCEEEEEHHHHHHHHCCCCHHHCEEEEECCCCCCHHH
RAVQIKSGRFGPYVTDGVTNATLDNTSWEDVTFDEAVSLLAEKRQRVDGDLDQETAEKIH
HEEEEECCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCC
V
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]