Click here to switch to the map view.

The map label for this gene is tmk

Identifier: 28572855

GI number: 28572855

Start: 807832

End: 808431

Strand: Reverse

Name: tmk

Synonym: TW714

Alternate gene names: 28572855

Gene position: 808431-807832 (Counterclockwise)

Preceding gene: 28572856

Following gene: 28572854

Centisome position: 87.31

GC content: 44.5

Gene sequence:

>600_bases
TTGTTTGTAACCTTTGAGGGTATTGATGGAAGCGGGAAGACGGCCCAGCTGACCGCGGCTGGAGAATTTCTAAAAGCGTC
AGGAAAACAGGTTGTCTTAACGAGGGAGCCGGGGACTATTTCTTGTATAAGGGATTATGTCCTTGCTTCTGATATGGATA
TCCGCACAGAGGCTTTACTATATTCGGCGGATAGAGCAGAGAATATAGCAAAAAATGTTATTCCGGCACTAAATGCCGGC
AAGGTGGTCTTGCAGGACAGATATCTGGACTCATTTCTGGCTTACCAGCTCGCAGATAATAAGCTAGTTGAGACGGACAT
TATGCGTCTTTTTGAGTTTTCCAGTCAGGGTCTTAGGCCCGATCTTACCTTATTGTTTGACCTTACTCCGGCTTGTGCGC
AAGAAAGGTTAGAGAATCGGGATCGAATCGAGAGAAAACCGATTGATTTTCATTCTAAGGTCCGTAATATATACCTCAAA
CTATCGGCAGATAATACGGATCGTATCAGGGTTATTGATGCGAGCCAATCCTTTTCTAAGATCCACCAACAGGTTATCTA
TCACATAGAAAGAAAGTTGGGCGGAACGCACTCAGCTTAA

Upstream 100 bases:

>100_bases
GTGATCTTGATCAAGAAACTGCAGAAAAGATCCATGTGTAGGATTTTACATATCGGATACACCGTCAGTTATTCTCGTAT
GGCCTCAAAGAGGGTTTTGC

Downstream 100 bases:

>100_bases
GTTCTAATCACAGGTGTCTATCTAAAACAGAGCAGTGCCAATTTGGATGTACTGAAATTGATATGCACTAGCTTTGTAAC
TTGCGCGGAATTTGATGTTC

Product: nucleotide kinase

Products: NA

Alternate protein names: dTMP kinase

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MFVTFEGIDGSGKTAQLTAAGEFLKASGKQVVLTREPGTISCIRDYVLASDMDIRTEALLYSADRAENIAKNVIPALNAG
KVVLQDRYLDSFLAYQLADNKLVETDIMRLFEFSSQGLRPDLTLLFDLTPACAQERLENRDRIERKPIDFHSKVRNIYLK
LSADNTDRIRVIDASQSFSKIHQQVIYHIERKLGGTHSA

Sequences:

>Translated_199_residues
MFVTFEGIDGSGKTAQLTAAGEFLKASGKQVVLTREPGTISCIRDYVLASDMDIRTEALLYSADRAENIAKNVIPALNAG
KVVLQDRYLDSFLAYQLADNKLVETDIMRLFEFSSQGLRPDLTLLFDLTPACAQERLENRDRIERKPIDFHSKVRNIYLK
LSADNTDRIRVIDASQSFSKIHQQVIYHIERKLGGTHSA
>Mature_199_residues
MFVTFEGIDGSGKTAQLTAAGEFLKASGKQVVLTREPGTISCIRDYVLASDMDIRTEALLYSADRAENIAKNVIPALNAG
KVVLQDRYLDSFLAYQLADNKLVETDIMRLFEFSSQGLRPDLTLLFDLTPACAQERLENRDRIERKPIDFHSKVRNIYLK
LSADNTDRIRVIDASQSFSKIHQQVIYHIERKLGGTHSA

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family

Homologues:

Organism=Escherichia coli, GI1787340, Length=193, Percent_Identity=32.1243523316062, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KTHY_TROW8 (Q83HB8)

Other databases:

- EMBL:   BX251412
- RefSeq:   NP_789635.1
- ProteinModelPortal:   Q83HB8
- STRING:   Q83HB8
- GeneID:   1064914
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW714
- eggNOG:   COG0125
- HOGENOM:   HBG626009
- OMA:   LFITLEG
- ProtClustDB:   CLSK230234
- BioCyc:   TWHI218496:TW0680-MONOMER
- HAMAP:   MF_00165
- InterPro:   IPR018094
- PANTHER:   PTHR10344
- TIGRFAMs:   TIGR00041

Pfam domain/function: PF02223 Thymidylate_kin

EC number: =2.7.4.9

Molecular weight: Translated: 22381; Mature: 22381

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVTFEGIDGSGKTAQLTAAGEFLKASGKQVVLTREPGTISCIRDYVLASDMDIRTEALL
CEEEEECCCCCCCEEEEEHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCHHHHHE
YSADRAENIAKNVIPALNAGKVVLQDRYLDSFLAYQLADNKLVETDIMRLFEFSSQGLRP
ECCHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC
DLTLLFDLTPACAQERLENRDRIERKPIDFHSKVRNIYLKLSADNTDRIRVIDASQSFSK
CEEEEEECCHHHHHHHHHHHHHHCCCCCCHHHHHEEEEEEEECCCCCEEEEEECCHHHHH
IHQQVIYHIERKLGGTHSA
HHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFVTFEGIDGSGKTAQLTAAGEFLKASGKQVVLTREPGTISCIRDYVLASDMDIRTEALL
CEEEEECCCCCCCEEEEEHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCHHHHHE
YSADRAENIAKNVIPALNAGKVVLQDRYLDSFLAYQLADNKLVETDIMRLFEFSSQGLRP
ECCHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC
DLTLLFDLTPACAQERLENRDRIERKPIDFHSKVRNIYLKLSADNTDRIRVIDASQSFSK
CEEEEEECCHHHHHHHHHHHHHHCCCCCCHHHHHEEEEEEEECCCCCEEEEEECCHHHHH
IHQQVIYHIERKLGGTHSA
HHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12606174