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The map label for this gene is recA

Identifier: 28572774

GI number: 28572774

Start: 715252

End: 716565

Strand: Reverse

Name: recA

Synonym: TW628

Alternate gene names: 28572774

Gene position: 716565-715252 (Counterclockwise)

Preceding gene: 28572775

Following gene: 28572773

Centisome position: 77.39

GC content: 48.86

Gene sequence:

>1314_bases
GTGTCATCTAACGATCAGATAAATCGCACGCGCGCGCTAGAGCTTGCTTTAGCAAATATAGATCGCCAATATGGCAAAGG
CGCTGTTATGCGCATGAACGATGCCCAGAAAGCGTATATAGACGTTATACCAACTGGTTCCATTGCACTTGATTATGCAC
TTGGGGTTGGAGGTCTGCCTCGCGGCCGGATTGTTGAGATATACGGTCCTGAATCAAGCGGTAAAACCACTTTGACTCTA
CACGCAATAGCAAGTGTCCAGCGCGCTGGTGGCATTGCTGCGTTCATAGATGCCGAGCATGCACTTGATCCGGAGTATGC
AAAAAAACTTGGGGTTGATATTGATGCGCTTCTTGTCAGCCAACCTGATAGCGGCGAGCAGGCATTGGAGATATCGGATA
TGTTAATTCGCTCAACAGCAATTGACCTGATTGTCATAGATTCCGTTGCTGCGCTTGTCCCGCGAGCTGAACTTGAGGGT
GATATGGGGGATATGCATGTTGGCCTCCAGGCACGGCTCATGTCTCAGGCTCTGAGAAAGATAACATCCGGGCTGAATCA
GACCGGAACGACCGCTATATTTATCAATCAACTTCGTGAAAAGGTTGGTGTTTTTTTTGGCAGTCCAGAAACAACTCCCG
GCGGCCGTGCTCTAAAGTTTTATGCTTCAGTTAGGCTTGATATTCGTCGAGTCGAAACCATCAAGCAGGGCACAGAGGCT
GTTGGAAACCGTACGCGCGTTAAGGTGGTGAAAAACAAAGTTGCACCGCCCTTCAAACAGGCCGAGTTCGACGTTCTGTA
CGGGGTTGGTATTTCCCGAGAAAGCGGCTTGATCGATTTTGCAGTTGATAGGGGAATCATTAAAAAATCAGGATCGTGGT
ACGTCTATGGCGATGATCAGTTGGGGCAGGGTAAGGAGAATGCTCGTCGGTTTCTCTTAGAGAATAAGAGCGTCGCGGAT
GAGATCGAGAATAAAATAATCAGCCTTTTAGGTTTCAGGTCGAACGAGTTCGGTGATTTGCCAGATCATGATGCCGCTGT
GCGCACTAGTCCTGACACAAATTCCCGTAAAGTGAGTGGAACTGGCGCCGTACACACAACATCCGGTAGCCCGTCCGCCG
GCAAAGGTACTGCCTCCGGTGCTGTAAACAATTCACGCGACTCTACTGGTGGCGATACACCGGCTGGCCAGGGCCCTTTA
AACTTATCTGTAAATCGTGACGTATCGACTGATACTGTTTCGTCTAAGATAAGTGATGCTACACATAATCAAAAGCCCGC
GGGTAATGGAAAAAGTGTTAAGAGAAAGGGTTAG

Upstream 100 bases:

>100_bases
TGTGGACGTTAACTTATGCACAAGATGGTGTTTTTGCTAGTTTTTTACCCGGTATTCATGTATTTTTCTTGTGTACGGAA
ACGGGAGGTTTGGAATAACG

Downstream 100 bases:

>100_bases
TTATCCCAAAACCGTTGACCTATACCGTTAGAACGTATGGATGTCAAATGAACGTCCATGACTCAGAGCGTATTGCTGGG
GTCCTGGAGGATGAGGGTTA

Product: recombinase A

Products: NA

Alternate protein names: Recombinase A

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MSSNDQINRTRALELALANIDRQYGKGAVMRMNDAQKAYIDVIPTGSIALDYALGVGGLPRGRIVEIYGPESSGKTTLTL
HAIASVQRAGGIAAFIDAEHALDPEYAKKLGVDIDALLVSQPDSGEQALEISDMLIRSTAIDLIVIDSVAALVPRAELEG
DMGDMHVGLQARLMSQALRKITSGLNQTGTTAIFINQLREKVGVFFGSPETTPGGRALKFYASVRLDIRRVETIKQGTEA
VGNRTRVKVVKNKVAPPFKQAEFDVLYGVGISRESGLIDFAVDRGIIKKSGSWYVYGDDQLGQGKENARRFLLENKSVAD
EIENKIISLLGFRSNEFGDLPDHDAAVRTSPDTNSRKVSGTGAVHTTSGSPSAGKGTASGAVNNSRDSTGGDTPAGQGPL
NLSVNRDVSTDTVSSKISDATHNQKPAGNGKSVKRKG

Sequences:

>Translated_437_residues
MSSNDQINRTRALELALANIDRQYGKGAVMRMNDAQKAYIDVIPTGSIALDYALGVGGLPRGRIVEIYGPESSGKTTLTL
HAIASVQRAGGIAAFIDAEHALDPEYAKKLGVDIDALLVSQPDSGEQALEISDMLIRSTAIDLIVIDSVAALVPRAELEG
DMGDMHVGLQARLMSQALRKITSGLNQTGTTAIFINQLREKVGVFFGSPETTPGGRALKFYASVRLDIRRVETIKQGTEA
VGNRTRVKVVKNKVAPPFKQAEFDVLYGVGISRESGLIDFAVDRGIIKKSGSWYVYGDDQLGQGKENARRFLLENKSVAD
EIENKIISLLGFRSNEFGDLPDHDAAVRTSPDTNSRKVSGTGAVHTTSGSPSAGKGTASGAVNNSRDSTGGDTPAGQGPL
NLSVNRDVSTDTVSSKISDATHNQKPAGNGKSVKRKG
>Mature_436_residues
SSNDQINRTRALELALANIDRQYGKGAVMRMNDAQKAYIDVIPTGSIALDYALGVGGLPRGRIVEIYGPESSGKTTLTLH
AIASVQRAGGIAAFIDAEHALDPEYAKKLGVDIDALLVSQPDSGEQALEISDMLIRSTAIDLIVIDSVAALVPRAELEGD
MGDMHVGLQARLMSQALRKITSGLNQTGTTAIFINQLREKVGVFFGSPETTPGGRALKFYASVRLDIRRVETIKQGTEAV
GNRTRVKVVKNKVAPPFKQAEFDVLYGVGISRESGLIDFAVDRGIIKKSGSWYVYGDDQLGQGKENARRFLLENKSVADE
IENKIISLLGFRSNEFGDLPDHDAAVRTSPDTNSRKVSGTGAVHTTSGSPSAGKGTASGAVNNSRDSTGGDTPAGQGPLN
LSVNRDVSTDTVSSKISDATHNQKPAGNGKSVKRKG

Specific function: Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family

Homologues:

Organism=Escherichia coli, GI1789051, Length=347, Percent_Identity=58.5014409221902, Blast_Score=415, Evalue=1e-117,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RECA_TROW8 (P65982)

Other databases:

- EMBL:   BX251412
- RefSeq:   NP_789554.1
- ProteinModelPortal:   P65982
- SMR:   P65982
- STRING:   P65982
- GeneID:   1064833
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW628
- eggNOG:   COG0468
- HOGENOM:   HBG339889
- OMA:   GRDNTIT
- PhylomeDB:   P65982
- ProtClustDB:   PRK09354
- BioCyc:   TWHI218496:TW0599-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00268
- InterPro:   IPR003593
- InterPro:   IPR013765
- InterPro:   IPR020584
- InterPro:   IPR020588
- InterPro:   IPR020587
- PANTHER:   PTHR22942:SF1
- PRINTS:   PR00142
- SMART:   SM00382
- TIGRFAMs:   TIGR02012

Pfam domain/function: PF00154 RecA

EC number: NA

Molecular weight: Translated: 46451; Mature: 46320

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS00321 RECA_1; PS50162 RECA_2; PS50163 RECA_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNDQINRTRALELALANIDRQYGKGAVMRMNDAQKAYIDVIPTGSIALDYALGVGGLP
CCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEECCCCEEEEEECCCCCCC
RGRIVEIYGPESSGKTTLTLHAIASVQRAGGIAAFIDAEHALDPEYAKKLGVDIDALLVS
CCCEEEEECCCCCCCEEEEEHHHHHHHHHCCEEEEECCHHCCCHHHHHHHCCCEEEEEEE
QPDSGEQALEISDMLIRSTAIDLIVIDSVAALVPRAELEGDMGDMHVGLQARLMSQALRK
CCCCCCHHHHHHHHHHHHHHHCEEEECHHHHHCCHHHCCCCCCCEEHHHHHHHHHHHHHH
ITSGLNQTGTTAIFINQLREKVGVFFGSPETTPGGRALKFYASVRLDIRRVETIKQGTEA
HHHCCCCCCCHHHHHHHHHHHHCEEECCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHH
VGNRTRVKVVKNKVAPPFKQAEFDVLYGVGISRESGLIDFAVDRGIIKKSGSWYVYGDDQ
HCCCEEEEEEHHCCCCCHHCCCEEEEEECCCCCCCCCEEEEECCCCEECCCCEEEEECCC
LGQGKENARRFLLENKSVADEIENKIISLLGFRSNEFGDLPDHDAAVRTSPDTNSRKVSG
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCEEECC
TGAVHTTSGSPSAGKGTASGAVNNSRDSTGGDTPAGQGPLNLSVNRDVSTDTVSSKISDA
CCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHC
THNQKPAGNGKSVKRKG
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSNDQINRTRALELALANIDRQYGKGAVMRMNDAQKAYIDVIPTGSIALDYALGVGGLP
CCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEECCCCEEEEEECCCCCCC
RGRIVEIYGPESSGKTTLTLHAIASVQRAGGIAAFIDAEHALDPEYAKKLGVDIDALLVS
CCCEEEEECCCCCCCEEEEEHHHHHHHHHCCEEEEECCHHCCCHHHHHHHCCCEEEEEEE
QPDSGEQALEISDMLIRSTAIDLIVIDSVAALVPRAELEGDMGDMHVGLQARLMSQALRK
CCCCCCHHHHHHHHHHHHHHHCEEEECHHHHHCCHHHCCCCCCCEEHHHHHHHHHHHHHH
ITSGLNQTGTTAIFINQLREKVGVFFGSPETTPGGRALKFYASVRLDIRRVETIKQGTEA
HHHCCCCCCCHHHHHHHHHHHHCEEECCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHH
VGNRTRVKVVKNKVAPPFKQAEFDVLYGVGISRESGLIDFAVDRGIIKKSGSWYVYGDDQ
HCCCEEEEEEHHCCCCCHHCCCEEEEEECCCCCCCCCEEEEECCCCEECCCCEEEEECCC
LGQGKENARRFLLENKSVADEIENKIISLLGFRSNEFGDLPDHDAAVRTSPDTNSRKVSG
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCEEECC
TGAVHTTSGSPSAGKGTASGAVNNSRDSTGGDTPAGQGPLNLSVNRDVSTDTVSSKISDA
CCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHC
THNQKPAGNGKSVKRKG
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12606174