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The map label for this gene is miaB

Identifier: 28572773

GI number: 28572773

Start: 713825

End: 715237

Strand: Reverse

Name: miaB

Synonym: TW627

Alternate gene names: 28572773

Gene position: 715237-713825 (Counterclockwise)

Preceding gene: 28572774

Following gene: 28572772

Centisome position: 77.24

GC content: 48.27

Gene sequence:

>1413_bases
TTGACCTATACCGTTAGAACGTATGGATGTCAAATGAACGTCCATGACTCAGAGCGTATTGCTGGGGTCCTGGAGGATGA
GGGTTATGTAAAATCCGGTAGTGACGATGCGGATGTTGTTGTTCTTAATACCTGTGCGGTCAGAGAGAATGCTGATAACA
GATTTTATGGCAATTTGGGGCAGCTTCTTCAAAAAAAGAATAACGGTCGGATCAGGCAGATAGCAGTTGGTGGGTGTTTG
GCCCAGAAAGATCGGCATAAGATTTTTGAAAAAGCCCCGTGGGTTGATGTTGTATTCGGAACCCACAATTTGGGCTCTCT
TCCTGCCCTGTTGCGCAGGTCGCGACACAATAAAACTGCAGAAATAGAAATAAAGGATTTTCTTGAGACTTTCCCATCCT
CTTTACCGGTAAGACGTGAATCCAATTATTCAGCATGGGTGTCTATCTCGGTTGGATGCAACAACACGTGCACTTTTTGT
ATTGTTCCAAGCTTGCGCGGCAAAGAGCGCGACAGGCGCCCGGGTGACATCCTAGCGGAAATAAGTGCTCTAGTCTCTGA
AGGTGTTCTCGAGGTTACCCTGCTTGGACAGAATGTCAATACATATGGAGTTGAATTTGGTGATAGGAGCGCTTTTGCAT
CCCTTCTCAGGAAAGCTGGAGCTATAGAGGGTCTTGAGCGACTCAAGTTTACAAGCCCGCATCCAGCTGCTTTTACGAGT
GACGTTATAGATGCGATGCACGATACCCAGGCTGTTCTGCCGCAATTGCATATGCCTCTTCAATCCGGTTCGGATCGCAT
TTTACGCGCGATGCGCCGTTCGTATAGAGCAGGCAAGTTCTTGAAAATAATCTCTGAGGCCCGCAACAAGATACCCAATA
TCGCAATTACAACAGATATCATTGTTGGCTTCCCGGGCGAAACAGAAGAGGATTTTCAGGACACCCTCAACCTTGTAGCC
GAGGTACGCTTCGCATCGGCGTTTACCTTTCAGTACTCTCCTCGCCCAGGAACCCCTGCTGCATCTATGCCTAACCAAAT
ACCAGGTGATATTGTCCAAGAACGGTATGACCGTTTACTGGACTTGCAAAATAGAATAGCCCTTGAGGAGAATCGGAAGC
TGATTGGAAAGGAGGTGGAGCTTCTTGTTACTGTTGGTGGCAGGAAAGATTCTATGCTGGATAATCGGTATACAGGTAGA
ACACCCTGTGGTAGGCTCGTCCACTTTTCGTGCCTACACCAACTGCGCCCGGGCGATTTTGCAACCGTAAAAATTATTTA
CGCCGCGCCGTACCACCTGATCGGGGATAATGCGCTTACGGTACGCAGAACACCAGCGGGCGATGTCTGGATTCAGAAGA
ACGACACGCGATCAGCGCAGCTTGTGTCGTTGGGTATGCCAAGAATTCGATAA

Upstream 100 bases:

>100_bases
CTGATACTGTTTCGTCTAAGATAAGTGATGCTACACATAATCAAAAGCCCGCGGGTAATGGAAAAAGTGTTAAGAGAAAG
GGTTAGTTATCCCAAAACCG

Downstream 100 bases:

>100_bases
CATAGATTATTCGATACCGTGAATTCGGTATTACCAGCGCTATATGGATTTGTTTCCTGGTTTTCTATTTCATAAGTGTT
AGCTGTAGCTTTTGTAGGCG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MTYTVRTYGCQMNVHDSERIAGVLEDEGYVKSGSDDADVVVLNTCAVRENADNRFYGNLGQLLQKKNNGRIRQIAVGGCL
AQKDRHKIFEKAPWVDVVFGTHNLGSLPALLRRSRHNKTAEIEIKDFLETFPSSLPVRRESNYSAWVSISVGCNNTCTFC
IVPSLRGKERDRRPGDILAEISALVSEGVLEVTLLGQNVNTYGVEFGDRSAFASLLRKAGAIEGLERLKFTSPHPAAFTS
DVIDAMHDTQAVLPQLHMPLQSGSDRILRAMRRSYRAGKFLKIISEARNKIPNIAITTDIIVGFPGETEEDFQDTLNLVA
EVRFASAFTFQYSPRPGTPAASMPNQIPGDIVQERYDRLLDLQNRIALEENRKLIGKEVELLVTVGGRKDSMLDNRYTGR
TPCGRLVHFSCLHQLRPGDFATVKIIYAAPYHLIGDNALTVRRTPAGDVWIQKNDTRSAQLVSLGMPRIR

Sequences:

>Translated_470_residues
MTYTVRTYGCQMNVHDSERIAGVLEDEGYVKSGSDDADVVVLNTCAVRENADNRFYGNLGQLLQKKNNGRIRQIAVGGCL
AQKDRHKIFEKAPWVDVVFGTHNLGSLPALLRRSRHNKTAEIEIKDFLETFPSSLPVRRESNYSAWVSISVGCNNTCTFC
IVPSLRGKERDRRPGDILAEISALVSEGVLEVTLLGQNVNTYGVEFGDRSAFASLLRKAGAIEGLERLKFTSPHPAAFTS
DVIDAMHDTQAVLPQLHMPLQSGSDRILRAMRRSYRAGKFLKIISEARNKIPNIAITTDIIVGFPGETEEDFQDTLNLVA
EVRFASAFTFQYSPRPGTPAASMPNQIPGDIVQERYDRLLDLQNRIALEENRKLIGKEVELLVTVGGRKDSMLDNRYTGR
TPCGRLVHFSCLHQLRPGDFATVKIIYAAPYHLIGDNALTVRRTPAGDVWIQKNDTRSAQLVSLGMPRIR
>Mature_469_residues
TYTVRTYGCQMNVHDSERIAGVLEDEGYVKSGSDDADVVVLNTCAVRENADNRFYGNLGQLLQKKNNGRIRQIAVGGCLA
QKDRHKIFEKAPWVDVVFGTHNLGSLPALLRRSRHNKTAEIEIKDFLETFPSSLPVRRESNYSAWVSISVGCNNTCTFCI
VPSLRGKERDRRPGDILAEISALVSEGVLEVTLLGQNVNTYGVEFGDRSAFASLLRKAGAIEGLERLKFTSPHPAAFTSD
VIDAMHDTQAVLPQLHMPLQSGSDRILRAMRRSYRAGKFLKIISEARNKIPNIAITTDIIVGFPGETEEDFQDTLNLVAE
VRFASAFTFQYSPRPGTPAASMPNQIPGDIVQERYDRLLDLQNRIALEENRKLIGKEVELLVTVGGRKDSMLDNRYTGRT
PCGRLVHFSCLHQLRPGDFATVKIIYAAPYHLIGDNALTVRRTPAGDVWIQKNDTRSAQLVSLGMPRIR

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=472, Percent_Identity=33.2627118644068, Blast_Score=248, Evalue=1e-65,
Organism=Homo sapiens, GI28872784, Length=472, Percent_Identity=33.2627118644068, Blast_Score=248, Evalue=1e-65,
Organism=Homo sapiens, GI93277076, Length=389, Percent_Identity=30.0771208226221, Blast_Score=173, Evalue=4e-43,
Organism=Escherichia coli, GI1786882, Length=445, Percent_Identity=40.6741573033708, Blast_Score=314, Evalue=7e-87,
Organism=Escherichia coli, GI1787057, Length=445, Percent_Identity=33.2584269662921, Blast_Score=221, Evalue=8e-59,
Organism=Caenorhabditis elegans, GI17553146, Length=468, Percent_Identity=32.6923076923077, Blast_Score=234, Evalue=5e-62,
Organism=Caenorhabditis elegans, GI71996771, Length=214, Percent_Identity=34.5794392523364, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI21356207, Length=428, Percent_Identity=37.6168224299065, Blast_Score=275, Evalue=7e-74,
Organism=Drosophila melanogaster, GI19922432, Length=374, Percent_Identity=31.283422459893, Blast_Score=178, Evalue=7e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_TROW8 (Q83HG3)

Other databases:

- EMBL:   BX251412
- RefSeq:   NP_789553.1
- ProteinModelPortal:   Q83HG3
- SMR:   Q83HG3
- STRING:   Q83HG3
- GeneID:   1064832
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW627
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- PhylomeDB:   Q83HG3
- ProtClustDB:   PRK14326
- BioCyc:   TWHI218496:TW0598-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 52329; Mature: 52198

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYTVRTYGCQMNVHDSERIAGVLEDEGYVKSGSDDADVVVLNTCAVRENADNRFYGNLG
CEEEEEEEEEEECCCCHHHHHEEECCCCEEECCCCCCCEEEEEEEEECCCCCCCCHHHHH
QLLQKKNNGRIRQIAVGGCLAQKDRHKIFEKAPWVDVVFGTHNLGSLPALLRRSRHNKTA
HHHHHCCCCCEEEEEECCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCEE
EIEIKDFLETFPSSLPVRRESNYSAWVSISVGCNNTCTFCIVPSLRGKERDRRPGDILAE
EEEHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCCCCCHHHHHH
ISALVSEGVLEVTLLGQNVNTYGVEFGDRSAFASLLRKAGAIEGLERLKFTSPHPAAFTS
HHHHHHCCCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
DVIDAMHDTQAVLPQLHMPLQSGSDRILRAMRRSYRAGKFLKIISEARNKIPNIAITTDI
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEE
IVGFPGETEEDFQDTLNLVAEVRFASAFTFQYSPRPGTPAASMPNQIPGDIVQERYDRLL
EEECCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHH
DLQNRIALEENRKLIGKEVELLVTVGGRKDSMLDNRYTGRTPCGRLVHFSCLHQLRPGDF
HHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCE
ATVKIIYAAPYHLIGDNALTVRRTPAGDVWIQKNDTRSAQLVSLGMPRIR
EEEEEEEECCEEEECCCEEEEEECCCCCEEEEECCCCCEEEEECCCCCCC
>Mature Secondary Structure 
TYTVRTYGCQMNVHDSERIAGVLEDEGYVKSGSDDADVVVLNTCAVRENADNRFYGNLG
EEEEEEEEEEECCCCHHHHHEEECCCCEEECCCCCCCEEEEEEEEECCCCCCCCHHHHH
QLLQKKNNGRIRQIAVGGCLAQKDRHKIFEKAPWVDVVFGTHNLGSLPALLRRSRHNKTA
HHHHHCCCCCEEEEEECCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCEE
EIEIKDFLETFPSSLPVRRESNYSAWVSISVGCNNTCTFCIVPSLRGKERDRRPGDILAE
EEEHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCCCCCHHHHHH
ISALVSEGVLEVTLLGQNVNTYGVEFGDRSAFASLLRKAGAIEGLERLKFTSPHPAAFTS
HHHHHHCCCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
DVIDAMHDTQAVLPQLHMPLQSGSDRILRAMRRSYRAGKFLKIISEARNKIPNIAITTDI
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEE
IVGFPGETEEDFQDTLNLVAEVRFASAFTFQYSPRPGTPAASMPNQIPGDIVQERYDRLL
EEECCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHH
DLQNRIALEENRKLIGKEVELLVTVGGRKDSMLDNRYTGRTPCGRLVHFSCLHQLRPGDF
HHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCE
ATVKIIYAAPYHLIGDNALTVRRTPAGDVWIQKNDTRSAQLVSLGMPRIR
EEEEEEEECCEEEECCCEEEEEECCCCCEEEEECCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12606174