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The map label for this gene is gspE [C]

Identifier: 28572731

GI number: 28572731

Start: 647813

End: 649147

Strand: Reverse

Name: gspE [C]

Synonym: TW583

Alternate gene names: 28572731

Gene position: 649147-647813 (Counterclockwise)

Preceding gene: 28572735

Following gene: 28572730

Centisome position: 70.11

GC content: 44.34

Gene sequence:

>1335_bases
GTGTTCTGGGCAACTGTCCACATTTTTATACGGACCTCGCATAAGTTACACAACCCAAGCCACCCTTGCCTGTTGAAAAG
TCAATTTCCTCCGCTTTTAAAGGTGCATTATATGAGTATGTCTGATGCGCTTGGGATTATAACGAAGAGGGTCAGGGACC
GTCTTCGTACCTCTGACCACGACCTTTCGTTGCAGCCCGAACTTACCGATCAATATGTCACTCGTGAACTTGAAGAGTAT
GTTAGCAGGCAACCAATTGATGAAACAGAAGACAGTATTAAAAGGAAAATAGTTGCAAACCTAACAGGATTTGGCCCACT
GCAGGACATCATTGATGATCCAGATGTTGAGGAATTCTGGATTAACTCGCCTGAAGAGGTATTTCTTGCCAAAAATGGTA
AATCCTTTGCAGTCGATATAAAGTTAACAGATTCTCAATTGAGGGATATTCTCGAAAGAATGCTGCAGGGAACCGGGCGG
CGCATTGATATGGCGAATCCTTTTGTTGATGCATCTTTACCCGATGGTAGTCGGCTGCATGCCGTTATACCAGATATTAC
GGCACGTCACATATCTATTAATGTTCGCAAATTTATGCGCCAGATTCGGGGTCTTGATCAGCTTGTTAGGACACGCAGTC
TGACTGCTGAATCCTCCAGATTTTTGCAATACTGCGTCAAGTCTCGTGCGAATTTTTTAGTCTCTGGCGCTACACAGGCT
GGCAAAACAACAATGCTCAATTCTCTTTTATCATGCTGCTCTGAGGATGAAAGGGTTGTAACAGTTGAGGAAACATTTGA
ACTTGATATTCCGCTGCGTGACGTGGTTAGCCTACAGTGTCGACAGGCAACAATTGAAGGATCCGGAGAAATAAATCTCA
GGCGTTTGGTCAAAGAAACTCTTAGAATGCGTCCTGATCGAATTGTTGTAGGTGAAGTGCGCGAGGCAGAAAGTCTTGAT
TTGTTAATTGCTCTCAATTCAGGCATTCCCGGTGCAGCGACTATACACGCTAACAGCGCACGTGATGCCCTCAGAAAAAT
GACAATCCTGCCTCTTTTGAGTGGTAAAAATATCGACTCAGCTTTTATAGTCCCAACGGTTGCTAATTGTATTGACATTG
TTGTGCATTGCGAGCTTGGCAAAGATGGGCATCGCAGGGTTTCTGAAATATTGGCACTCAGTGGTCGGGTGAATGAAGGC
ATTATCGAGGCAAGTCCTGTATTTACCACAACAAATAATCAATTGCAGTTCACAGGCATCTACCCAGAACAATGGAGGGG
GTTCATGAATGAACAGGGCTCCACTGTATTTAAGAACTTGGAGACTGATGCTTAG

Upstream 100 bases:

>100_bases
ACTGCGCATCCAGCCTGCACTAACCATGCCTGAGGTACTAACCATGCCTGAAGTATGGTGCTGCACACATTTCATTTACG
CGGTGTTCTGAGGTCACGCG

Downstream 100 bases:

>100_bases
CGTGGCGCTTTCTTTGTTACTATCAGCAGGTAATCTATTTCTCCTTGCCAAATCAAAACCGTCAAGGCGGTACAGCAGAT
TCGATAAGTTTCATTCACGT

Product: TadA-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MFWATVHIFIRTSHKLHNPSHPCLLKSQFPPLLKVHYMSMSDALGIITKRVRDRLRTSDHDLSLQPELTDQYVTRELEEY
VSRQPIDETEDSIKRKIVANLTGFGPLQDIIDDPDVEEFWINSPEEVFLAKNGKSFAVDIKLTDSQLRDILERMLQGTGR
RIDMANPFVDASLPDGSRLHAVIPDITARHISINVRKFMRQIRGLDQLVRTRSLTAESSRFLQYCVKSRANFLVSGATQA
GKTTMLNSLLSCCSEDERVVTVEETFELDIPLRDVVSLQCRQATIEGSGEINLRRLVKETLRMRPDRIVVGEVREAESLD
LLIALNSGIPGAATIHANSARDALRKMTILPLLSGKNIDSAFIVPTVANCIDIVVHCELGKDGHRRVSEILALSGRVNEG
IIEASPVFTTTNNQLQFTGIYPEQWRGFMNEQGSTVFKNLETDA

Sequences:

>Translated_444_residues
MFWATVHIFIRTSHKLHNPSHPCLLKSQFPPLLKVHYMSMSDALGIITKRVRDRLRTSDHDLSLQPELTDQYVTRELEEY
VSRQPIDETEDSIKRKIVANLTGFGPLQDIIDDPDVEEFWINSPEEVFLAKNGKSFAVDIKLTDSQLRDILERMLQGTGR
RIDMANPFVDASLPDGSRLHAVIPDITARHISINVRKFMRQIRGLDQLVRTRSLTAESSRFLQYCVKSRANFLVSGATQA
GKTTMLNSLLSCCSEDERVVTVEETFELDIPLRDVVSLQCRQATIEGSGEINLRRLVKETLRMRPDRIVVGEVREAESLD
LLIALNSGIPGAATIHANSARDALRKMTILPLLSGKNIDSAFIVPTVANCIDIVVHCELGKDGHRRVSEILALSGRVNEG
IIEASPVFTTTNNQLQFTGIYPEQWRGFMNEQGSTVFKNLETDA
>Mature_444_residues
MFWATVHIFIRTSHKLHNPSHPCLLKSQFPPLLKVHYMSMSDALGIITKRVRDRLRTSDHDLSLQPELTDQYVTRELEEY
VSRQPIDETEDSIKRKIVANLTGFGPLQDIIDDPDVEEFWINSPEEVFLAKNGKSFAVDIKLTDSQLRDILERMLQGTGR
RIDMANPFVDASLPDGSRLHAVIPDITARHISINVRKFMRQIRGLDQLVRTRSLTAESSRFLQYCVKSRANFLVSGATQA
GKTTMLNSLLSCCSEDERVVTVEETFELDIPLRDVVSLQCRQATIEGSGEINLRRLVKETLRMRPDRIVVGEVREAESLD
LLIALNSGIPGAATIHANSARDALRKMTILPLLSGKNIDSAFIVPTVANCIDIVVHCELGKDGHRRVSEILALSGRVNEG
IIEASPVFTTTNNQLQFTGIYPEQWRGFMNEQGSTVFKNLETDA

Specific function: Involved In A General Secretion Pathway (Gsp) For The Export Of Proteins (By Similarity). [C]

COG id: COG4962

COG function: function code U; Flp pilus assembly protein, ATPase CpaF

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022399
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 49919; Mature: 49919

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFWATVHIFIRTSHKLHNPSHPCLLKSQFPPLLKVHYMSMSDALGIITKRVRDRLRTSDH
CEEEEEEEEEEECCCCCCCCCCEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCC
DLSLQPELTDQYVTRELEEYVSRQPIDETEDSIKRKIVANLTGFGPLQDIIDDPDVEEFW
CCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHH
INSPEEVFLAKNGKSFAVDIKLTDSQLRDILERMLQGTGRRIDMANPFVDASLPDGSRLH
CCCCCEEEEEECCCEEEEEEEECHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCEE
AVIPDITARHISINVRKFMRQIRGLDQLVRTRSLTAESSRFLQYCVKSRANFLVSGATQA
EECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECCCCC
GKTTMLNSLLSCCSEDERVVTVEETFELDIPLRDVVSLQCRQATIEGSGEINLRRLVKET
CHHHHHHHHHHHCCCCCEEEEEEECEEECCCHHHHHHHHHHHEEECCCCCCHHHHHHHHH
LRMRPDRIVVGEVREAESLDLLIALNSGIPGAATIHANSARDALRKMTILPLLSGKNIDS
HHCCCCEEEEECCCCCCCCEEEEEECCCCCCEEEEECCHHHHHHHHHHHEEEECCCCCCC
AFIVPTVANCIDIVVHCELGKDGHRRVSEILALSGRVNEGIIEASPVFTTTNNQLQFTGI
EEEHHHHHHHEEEEEEEECCCCHHHHHHHHHHHCCCCCCCEEECCCEEEECCCEEEEEEC
YPEQWRGFMNEQGSTVFKNLETDA
CHHHHHHHHCCCCCHHHHHCCCCC
>Mature Secondary Structure
MFWATVHIFIRTSHKLHNPSHPCLLKSQFPPLLKVHYMSMSDALGIITKRVRDRLRTSDH
CEEEEEEEEEEECCCCCCCCCCEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCC
DLSLQPELTDQYVTRELEEYVSRQPIDETEDSIKRKIVANLTGFGPLQDIIDDPDVEEFW
CCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHH
INSPEEVFLAKNGKSFAVDIKLTDSQLRDILERMLQGTGRRIDMANPFVDASLPDGSRLH
CCCCCEEEEEECCCEEEEEEEECHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCEE
AVIPDITARHISINVRKFMRQIRGLDQLVRTRSLTAESSRFLQYCVKSRANFLVSGATQA
EECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECCCCC
GKTTMLNSLLSCCSEDERVVTVEETFELDIPLRDVVSLQCRQATIEGSGEINLRRLVKET
CHHHHHHHHHHHCCCCCEEEEEEECEEECCCHHHHHHHHHHHEEECCCCCCHHHHHHHHH
LRMRPDRIVVGEVREAESLDLLIALNSGIPGAATIHANSARDALRKMTILPLLSGKNIDS
HHCCCCEEEEECCCCCCCCEEEEEECCCCCCEEEEECCHHHHHHHHHHHEEEECCCCCCC
AFIVPTVANCIDIVVHCELGKDGHRRVSEILALSGRVNEGIIEASPVFTTTNNQLQFTGI
EEEHHHHHHHEEEEEEEECCCCHHHHHHHHHHHCCCCCCCEEECCCEEEECCCEEEEEEC
YPEQWRGFMNEQGSTVFKNLETDA
CHHHHHHHHCCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036; 9634230 [H]