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The map label for this gene is pyrB [H]

Identifier: 28572724

GI number: 28572724

Start: 634418

End: 635638

Strand: Reverse

Name: pyrB [H]

Synonym: TW576

Alternate gene names: 28572724

Gene position: 635638-634418 (Counterclockwise)

Preceding gene: 28572725

Following gene: 28572722

Centisome position: 68.65

GC content: 45.54

Gene sequence:

>1221_bases
GTGACTGCTCATAATACCCCGAAGGTTCATTGTGTTATCCGCCAGGCTTTACGAGGTCAGCGCATAGGCCCCCGGCAGCT
TGCCGAGCAAATTGGTGTTGACATCAAACAACTTTGGTCATGGCTGGAACCCACATGGTCGCAAACACTTGACATCCCGC
AGTCCATTCTGCATGACCTGGAAGATGCTATTGATGACACATCCGCGTTTTACAATACTCAACACTTGTTATCCATTGAC
CAAATAAATCGCAGAAGCCTTGAGAGATTATTTTATGTAACAGATATGTTTCAGCCCCTTGCAAGGCGTCAGGTGAGGTG
CAACATTCTGAACGGCGCGGTCCTTGCCTGTCTTTTTTACGAAGCTAGTACGCGTACAAGATTTAGTTTTTCGACTGCTT
TTTCCCGGATGGGTGGGGCCGTTGTGACAGAGGCCGATATGCTTCAGTCTTCCTCGGTTGCAAAAGGGGAATCAACCTCA
GACACCAGTCGAGTTATTTCTGGGTATGCGGATATTGTAGTTGTTCGGCATTCAGAACTTGGATTTGCTGAAAGATACGC
CTCTGACAGCTTGGTTCCGGTTATAAATGCCGGAGATGGTTCGGGCGAGCACCCGACCCAGGCGATTCTGGATGTATACA
CAATAGAAAGTGAATTTCTTTACAGAGAGAAAACAATCGATGGAAGCTGTATTGCAATCATAGGTGATCTAAAACACGGC
AGAACCGTTCATTCACTGATTAAGCTTTTATCTCTTTATGAGAGATTAACATTCAAAATATTTTCTATAACAGATCTTGA
GCTTCCGAATGATTTTCATGATCTCCTGTCTGGTCGTGGTCATACCCTAGAGGTTTGTGACAGCTTGAGGAATGCTGTCA
ATGCCGCAGATGTTATTTATGCAACGCGTGTTCAAACAGAAAGGATTGCGCAGGACTTGACAGCTGTAACGGGGTGTAGG
CTGACACGGGAAGTGCTTGATGATTGTAAAAAGGATGTGATTGTATTGCATCCACTTCCACGTGATTCCCGCCCAGATGC
TCTAGACATAGGAACTGATCTTGATTCGTACAGTGGGTTTATGCCATTTGGTCAAGCGAATAATGGGGTTCCTGTTAGAA
TGGCAATCTTCTCAGAAATCCTGGGGATAACCGACAAAATAGAGTTCGCAGATTCTATACCATGGGCAAGAAGAATACCT
TACCCCCAGGCCTTACCCTAA

Upstream 100 bases:

>100_bases
ACTATCCCAGTAGTTTCAAGAACAGTTTCACCCACTCAGTTACCCTGTGGAACTACAAAAGCACCGCAAAATCCCAAGGA
AGGCTTATTAAACTGGTAAT

Downstream 100 bases:

>100_bases
ACACAAGTACAGTACCACAGTATCTATTCACATATACAAGAAATACTACACAGACATATGCAGTAACTCACTGCCAAGTA
TTCATGCTGTGACTTCGACT

Product: aspartate carbamoyltransferase

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MTAHNTPKVHCVIRQALRGQRIGPRQLAEQIGVDIKQLWSWLEPTWSQTLDIPQSILHDLEDAIDDTSAFYNTQHLLSID
QINRRSLERLFYVTDMFQPLARRQVRCNILNGAVLACLFYEASTRTRFSFSTAFSRMGGAVVTEADMLQSSSVAKGESTS
DTSRVISGYADIVVVRHSELGFAERYASDSLVPVINAGDGSGEHPTQAILDVYTIESEFLYREKTIDGSCIAIIGDLKHG
RTVHSLIKLLSLYERLTFKIFSITDLELPNDFHDLLSGRGHTLEVCDSLRNAVNAADVIYATRVQTERIAQDLTAVTGCR
LTREVLDDCKKDVIVLHPLPRDSRPDALDIGTDLDSYSGFMPFGQANNGVPVRMAIFSEILGITDKIEFADSIPWARRIP
YPQALP

Sequences:

>Translated_406_residues
MTAHNTPKVHCVIRQALRGQRIGPRQLAEQIGVDIKQLWSWLEPTWSQTLDIPQSILHDLEDAIDDTSAFYNTQHLLSID
QINRRSLERLFYVTDMFQPLARRQVRCNILNGAVLACLFYEASTRTRFSFSTAFSRMGGAVVTEADMLQSSSVAKGESTS
DTSRVISGYADIVVVRHSELGFAERYASDSLVPVINAGDGSGEHPTQAILDVYTIESEFLYREKTIDGSCIAIIGDLKHG
RTVHSLIKLLSLYERLTFKIFSITDLELPNDFHDLLSGRGHTLEVCDSLRNAVNAADVIYATRVQTERIAQDLTAVTGCR
LTREVLDDCKKDVIVLHPLPRDSRPDALDIGTDLDSYSGFMPFGQANNGVPVRMAIFSEILGITDKIEFADSIPWARRIP
YPQALP
>Mature_405_residues
TAHNTPKVHCVIRQALRGQRIGPRQLAEQIGVDIKQLWSWLEPTWSQTLDIPQSILHDLEDAIDDTSAFYNTQHLLSIDQ
INRRSLERLFYVTDMFQPLARRQVRCNILNGAVLACLFYEASTRTRFSFSTAFSRMGGAVVTEADMLQSSSVAKGESTSD
TSRVISGYADIVVVRHSELGFAERYASDSLVPVINAGDGSGEHPTQAILDVYTIESEFLYREKTIDGSCIAIIGDLKHGR
TVHSLIKLLSLYERLTFKIFSITDLELPNDFHDLLSGRGHTLEVCDSLRNAVNAADVIYATRVQTERIAQDLTAVTGCRL
TREVLDDCKKDVIVLHPLPRDSRPDALDIGTDLDSYSGFMPFGQANNGVPVRMAIFSEILGITDKIEFADSIPWARRIPY
PQALP

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=312, Percent_Identity=40.3846153846154, Blast_Score=226, Evalue=3e-59,
Organism=Escherichia coli, GI2367364, Length=313, Percent_Identity=35.1437699680511, Blast_Score=178, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI193204318, Length=329, Percent_Identity=40.7294832826748, Blast_Score=204, Evalue=7e-53,
Organism=Saccharomyces cerevisiae, GI6322331, Length=317, Percent_Identity=35.0157728706625, Blast_Score=177, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24642586, Length=313, Percent_Identity=38.9776357827476, Blast_Score=206, Evalue=2e-53,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 45304; Mature: 45173

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAHNTPKVHCVIRQALRGQRIGPRQLAEQIGVDIKQLWSWLEPTWSQTLDIPQSILHDL
CCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHCHHHHHHHCCHHHHHHHH
EDAIDDTSAFYNTQHLLSIDQINRRSLERLFYVTDMFQPLARRQVRCNILNGAVLACLFY
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EASTRTRFSFSTAFSRMGGAVVTEADMLQSSSVAKGESTSDTSRVISGYADIVVVRHSEL
HCCCCCEEHHHHHHHHHCCCCEEHHHHHHCCCCCCCCCCCHHHHHHHCCEEEEEEEECCC
GFAERYASDSLVPVINAGDGSGEHPTQAILDVYTIESEFLYREKTIDGSCIAIIGDLKHG
CHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEECCCCC
RTVHSLIKLLSLYERLTFKIFSITDLELPNDFHDLLSGRGHTLEVCDSLRNAVNAADVIY
HHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
ATRVQTERIAQDLTAVTGCRLTREVLDDCKKDVIVLHPLPRDSRPDALDIGTDLDSYSGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEECCCCCHHCCCC
MPFGQANNGVPVRMAIFSEILGITDKIEFADSIPWARRIPYPQALP
CCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHCCCHHHCCCCCCCCC
>Mature Secondary Structure 
TAHNTPKVHCVIRQALRGQRIGPRQLAEQIGVDIKQLWSWLEPTWSQTLDIPQSILHDL
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHCHHHHHHHCCHHHHHHHH
EDAIDDTSAFYNTQHLLSIDQINRRSLERLFYVTDMFQPLARRQVRCNILNGAVLACLFY
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EASTRTRFSFSTAFSRMGGAVVTEADMLQSSSVAKGESTSDTSRVISGYADIVVVRHSEL
HCCCCCEEHHHHHHHHHCCCCEEHHHHHHCCCCCCCCCCCHHHHHHHCCEEEEEEEECCC
GFAERYASDSLVPVINAGDGSGEHPTQAILDVYTIESEFLYREKTIDGSCIAIIGDLKHG
CHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEECCCCC
RTVHSLIKLLSLYERLTFKIFSITDLELPNDFHDLLSGRGHTLEVCDSLRNAVNAADVIY
HHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
ATRVQTERIAQDLTAVTGCRLTREVLDDCKKDVIVLHPLPRDSRPDALDIGTDLDSYSGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEECCCCCHHCCCC
MPFGQANNGVPVRMAIFSEILGITDKIEFADSIPWARRIPYPQALP
CCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA