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The map label for this gene is prfB [H]

Identifier: 28572722

GI number: 28572722

Start: 627148

End: 628278

Strand: Reverse

Name: prfB [H]

Synonym: TW574

Alternate gene names: 28572722

Gene position: 628278-627148 (Counterclockwise)

Preceding gene: 28572724

Following gene: 28572721

Centisome position: 67.85

GC content: 46.86

Gene sequence:

>1131_bases
ATGAATATTACCGAACTTTTTGATTCCCTGAAGGAAATCGAGAAGACCTTCAGTGAAATAACAAGTGTTGTTTCGCCAGT
TTTGCTCTCAAAGAGAATAGCAGAACTAACCGCTCGCGTGTCGGATCCAGATCTTTGGACCACACCCGACAGGGCACAAC
AGATCGCGCGTGAGCTCAGCATTACAAAAGCCCAACTTGATCGCCTAACAAATCTTGAAGCCCAACTTGATGAAATGCGT
GTTCTGGTTACTCTATTAGAGAATGAGCATGACGCTGGCCTGCTTGATGAGGGCAGCAGAAAGGCGAAATTACTCCTTGA
GGAGGCCAACCACCTGTTGATTCAAAACATGCTAAATAATGAGTACGATTGCTATCCTGCAGTGCTTACAATCCGGGCAG
GAGCGGGCGGTATTGATGCCGCCGATTTCGCACAGATGCTCTTGCGAATGTATATACGGTGGGCCCAAAAACACTCTTCC
GAAGCGATTGTAATGGACATAAGCTATGCCGAAGAGGCCGGCATTCGTTCGGCAACCCTGCGGATTGATCTACCATTTGC
TTTTGGTAAATTACATCAAGAGTCAGGAACTCATAGGCTGGTTCGGATTAGCCCGTTTAATGCAGCCGGAAAAAGACATA
CAAGCTTTGCTGCAGTTGAGGTAATACCTCTCCTAGAGGAGGTATCTTCTATCGAAATAACTAATGATGAAATCAGAATA
GATGTCTTTAGATCATCAGGACCCGGAGGACAGTCTGTTAACACAACAGACTCAGCAGTCAGAATAACCCATCTCCCAAC
AGGAATTGTAGTCTCTTGCCAAAACGAGAAAAGCCAGATACAAAACCGAGAATCAGCAATGCGGATCCTGCGAGCGAGGC
TGCTCCTTAACAAGAGACAGGAAGAGGCTGCCATTCGTAAGGAGCTTGCCGGAAACATAGTTGCAAGCTGGGGTGAGCAG
GTGCGCAATTATGTTCTCGCGCCTTATCAGCTTGTAAAGGATCTTCGCAGTCTGCACGAAAGTTCCATGCCGGACAAAGT
GTTTGACGGTGACATTGATGGCTTCATTGATGCCGGAATAAAATGGCACGCGCAAAAACACATACTAAACCAAAATGGCA
CGCGCGAATAG

Upstream 100 bases:

>100_bases
AGGATAGAACCGATGGGATAAAAACCACTATTATTGTTGGGAGTAGCTGTTTTATGTTACTTACCTTTCGTTTCTCACTG
TTTTTGCAAAATAGCGCAGT

Downstream 100 bases:

>100_bases
AACATTCGGAAACCGGCGTGACAGACAAACATGACTCGGGCATAAACAACCACAAAGCACACAGGGCAGCAGACAACACA
GTGCCAGACAGTACAACACA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MNITELFDSLKEIEKTFSEITSVVSPVLLSKRIAELTARVSDPDLWTTPDRAQQIARELSITKAQLDRLTNLEAQLDEMR
VLVTLLENEHDAGLLDEGSRKAKLLLEEANHLLIQNMLNNEYDCYPAVLTIRAGAGGIDAADFAQMLLRMYIRWAQKHSS
EAIVMDISYAEEAGIRSATLRIDLPFAFGKLHQESGTHRLVRISPFNAAGKRHTSFAAVEVIPLLEEVSSIEITNDEIRI
DVFRSSGPGGQSVNTTDSAVRITHLPTGIVVSCQNEKSQIQNRESAMRILRARLLLNKRQEEAAIRKELAGNIVASWGEQ
VRNYVLAPYQLVKDLRSLHESSMPDKVFDGDIDGFIDAGIKWHAQKHILNQNGTRE

Sequences:

>Translated_376_residues
MNITELFDSLKEIEKTFSEITSVVSPVLLSKRIAELTARVSDPDLWTTPDRAQQIARELSITKAQLDRLTNLEAQLDEMR
VLVTLLENEHDAGLLDEGSRKAKLLLEEANHLLIQNMLNNEYDCYPAVLTIRAGAGGIDAADFAQMLLRMYIRWAQKHSS
EAIVMDISYAEEAGIRSATLRIDLPFAFGKLHQESGTHRLVRISPFNAAGKRHTSFAAVEVIPLLEEVSSIEITNDEIRI
DVFRSSGPGGQSVNTTDSAVRITHLPTGIVVSCQNEKSQIQNRESAMRILRARLLLNKRQEEAAIRKELAGNIVASWGEQ
VRNYVLAPYQLVKDLRSLHESSMPDKVFDGDIDGFIDAGIKWHAQKHILNQNGTRE
>Mature_376_residues
MNITELFDSLKEIEKTFSEITSVVSPVLLSKRIAELTARVSDPDLWTTPDRAQQIARELSITKAQLDRLTNLEAQLDEMR
VLVTLLENEHDAGLLDEGSRKAKLLLEEANHLLIQNMLNNEYDCYPAVLTIRAGAGGIDAADFAQMLLRMYIRWAQKHSS
EAIVMDISYAEEAGIRSATLRIDLPFAFGKLHQESGTHRLVRISPFNAAGKRHTSFAAVEVIPLLEEVSSIEITNDEIRI
DVFRSSGPGGQSVNTTDSAVRITHLPTGIVVSCQNEKSQIQNRESAMRILRARLLLNKRQEEAAIRKELAGNIVASWGEQ
VRNYVLAPYQLVKDLRSLHESSMPDKVFDGDIDGFIDAGIKWHAQKHILNQNGTRE

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=227, Percent_Identity=36.5638766519824, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI34577120, Length=229, Percent_Identity=36.2445414847162, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI166795305, Length=156, Percent_Identity=35.8974358974359, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI2367172, Length=332, Percent_Identity=40.3614457831325, Blast_Score=259, Evalue=3e-70,
Organism=Escherichia coli, GI1787462, Length=241, Percent_Identity=40.2489626556017, Blast_Score=163, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17542784, Length=190, Percent_Identity=35.2631578947368, Blast_Score=95, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6321295, Length=177, Percent_Identity=45.1977401129944, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI19921226, Length=261, Percent_Identity=34.8659003831418, Blast_Score=140, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 42089; Mature: 42089

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNITELFDSLKEIEKTFSEITSVVSPVLLSKRIAELTARVSDPDLWTTPDRAQQIARELS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
ITKAQLDRLTNLEAQLDEMRVLVTLLENEHDAGLLDEGSRKAKLLLEEANHLLIQNMLNN
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
EYDCYPAVLTIRAGAGGIDAADFAQMLLRMYIRWAQKHSSEAIVMDISYAEEAGIRSATL
CCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHCCCCEEEE
RIDLPFAFGKLHQESGTHRLVRISPFNAAGKRHTSFAAVEVIPLLEEVSSIEITNDEIRI
EEECCHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCEEEECCEEEE
DVFRSSGPGGQSVNTTDSAVRITHLPTGIVVSCQNEKSQIQNRESAMRILRARLLLNKRQ
EEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCHH
EEAAIRKELAGNIVASWGEQVRNYVLAPYQLVKDLRSLHESSMPDKVFDGDIDGFIDAGI
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCC
KWHAQKHILNQNGTRE
HHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNITELFDSLKEIEKTFSEITSVVSPVLLSKRIAELTARVSDPDLWTTPDRAQQIARELS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
ITKAQLDRLTNLEAQLDEMRVLVTLLENEHDAGLLDEGSRKAKLLLEEANHLLIQNMLNN
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
EYDCYPAVLTIRAGAGGIDAADFAQMLLRMYIRWAQKHSSEAIVMDISYAEEAGIRSATL
CCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHCCCCEEEE
RIDLPFAFGKLHQESGTHRLVRISPFNAAGKRHTSFAAVEVIPLLEEVSSIEITNDEIRI
EEECCHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCEEEECCEEEE
DVFRSSGPGGQSVNTTDSAVRITHLPTGIVVSCQNEKSQIQNRESAMRILRARLLLNKRQ
EEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCHH
EEAAIRKELAGNIVASWGEQVRNYVLAPYQLVKDLRSLHESSMPDKVFDGDIDGFIDAGI
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCC
KWHAQKHILNQNGTRE
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA