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The map label for this gene is ilvE [H]

Identifier: 28572712

GI number: 28572712

Start: 602111

End: 603196

Strand: Reverse

Name: ilvE [H]

Synonym: TW564

Alternate gene names: 28572712

Gene position: 603196-602111 (Counterclockwise)

Preceding gene: 28572713

Following gene: 28572711

Centisome position: 65.14

GC content: 47.61

Gene sequence:

>1086_bases
TTGAGTGCCAAGAATTATAAAAAAACACTGCTAGTAAACCCCATTTCGCAGGATGAATTACAAAAGAGGCTTTCTGCCCC
AGGGTTCGGGTCTGTTTTTTCTGAGCATATGGTCTCCGCGGATTGGCATCGCGATCTTGGTTGGCATGATTATCGTGTCG
AACCGTATGGTCCAATTAGTCTTGCCCCCTCGGCCATGGTATTTCATTATGGACAGGAAATTTTCGAGGGGCTGAAAATT
TTTCGCCATTCACAGGATAGTTCTGTGCGCGCATTTAGGCCACTGGAAAATGCAAAGAGGTTTCAAAAATCCTCCGAGAG
AATCGCGCTTCCCGTTTTACCGGTTGAGGATTTTCTGCAATCGCTTCGTGAGTGGGTGATGGTTGACGGCCGCTGGGTTC
CGAATGCACCGTCATGTGTTTATGCAAGACCATTTATGATAGCAACCGAGGAGGCGCTCGGTCTTCGGGCAACGTGCAGC
GCCAGATATACCCTAATTTGCTGTCCGGTTGGTGATTTGTTTGGCCAAATAAGGCCCATTAAGGTACGGGTTTCTTCTAA
GTATTCGAGGGCGTCCAGGGGTGGGACCGGGTCCGTTAAATTTGGTGGTAATTATGCGGCATCTATTTTGCCCCAGGTCG
AGGCTGGGAATAATGGGTTTGACCAGGTTCTTTTTTTGGACTCGGCATCTTATAAAAATATTGAAGAGCTGTCCGCATCG
AACATATTCTTTGTACTCTCCAGTAAGACTTTGATTACGCCGAGTCTTGATGGAACTATTCTTCCGGGTGTTACTCGTGA
CAGTATCATAAGAATAGCCGAGGATTTGGGGTTCGTTGTTGAGCAAAGACAAATCAGTCTTGACGAGGTAACACATCTAT
TTAGTCAGGACAAAGTAACGGAAATGTTTTGCTGCGGAACCGCGGCGTCTGTAACCCCCATTGGGTCCGTGTCTTTTGAT
GGAGGCACCCTGGGCTCGGGGCAGGCGGGTCCTGTTACGATGCTCCTTCGTGAATATCTGCGGGATATACAGTTTGGTAA
TCGTCCGGATAAATACAACTGGATGGTCAATCTTGAGGATACTTAG

Upstream 100 bases:

>100_bases
AGAGGTTATCGGTGTATTTGGTCTGCGCTGTATATGTTGCGCCGTGGCCTTCTTGGTTATTGCCAAATGCGGCTATTTGG
ATGTGTGAAATGGAAAAGCC

Downstream 100 bases:

>100_bases
GTGAGTTTTTGGTCTGTAAGAAACACTTGAACAGACGGGGGTGTTTTTGTGCGTGACCCGCGTGTGCGGGTTAGATTTTG
CCCGTCTCCAACTGGTGCAC

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MSAKNYKKTLLVNPISQDELQKRLSAPGFGSVFSEHMVSADWHRDLGWHDYRVEPYGPISLAPSAMVFHYGQEIFEGLKI
FRHSQDSSVRAFRPLENAKRFQKSSERIALPVLPVEDFLQSLREWVMVDGRWVPNAPSCVYARPFMIATEEALGLRATCS
ARYTLICCPVGDLFGQIRPIKVRVSSKYSRASRGGTGSVKFGGNYAASILPQVEAGNNGFDQVLFLDSASYKNIEELSAS
NIFFVLSSKTLITPSLDGTILPGVTRDSIIRIAEDLGFVVEQRQISLDEVTHLFSQDKVTEMFCCGTAASVTPIGSVSFD
GGTLGSGQAGPVTMLLREYLRDIQFGNRPDKYNWMVNLEDT

Sequences:

>Translated_361_residues
MSAKNYKKTLLVNPISQDELQKRLSAPGFGSVFSEHMVSADWHRDLGWHDYRVEPYGPISLAPSAMVFHYGQEIFEGLKI
FRHSQDSSVRAFRPLENAKRFQKSSERIALPVLPVEDFLQSLREWVMVDGRWVPNAPSCVYARPFMIATEEALGLRATCS
ARYTLICCPVGDLFGQIRPIKVRVSSKYSRASRGGTGSVKFGGNYAASILPQVEAGNNGFDQVLFLDSASYKNIEELSAS
NIFFVLSSKTLITPSLDGTILPGVTRDSIIRIAEDLGFVVEQRQISLDEVTHLFSQDKVTEMFCCGTAASVTPIGSVSFD
GGTLGSGQAGPVTMLLREYLRDIQFGNRPDKYNWMVNLEDT
>Mature_360_residues
SAKNYKKTLLVNPISQDELQKRLSAPGFGSVFSEHMVSADWHRDLGWHDYRVEPYGPISLAPSAMVFHYGQEIFEGLKIF
RHSQDSSVRAFRPLENAKRFQKSSERIALPVLPVEDFLQSLREWVMVDGRWVPNAPSCVYARPFMIATEEALGLRATCSA
RYTLICCPVGDLFGQIRPIKVRVSSKYSRASRGGTGSVKFGGNYAASILPQVEAGNNGFDQVLFLDSASYKNIEELSASN
IFFVLSSKTLITPSLDGTILPGVTRDSIIRIAEDLGFVVEQRQISLDEVTHLFSQDKVTEMFCCGTAASVTPIGSVSFDG
GTLGSGQAGPVTMLLREYLRDIQFGNRPDKYNWMVNLEDT

Specific function: Catalyzes the reversible transfers of an amino group from glutamate to the alpha-ketoacid of the respective amino acid in the final step in the biosynthesis of branchedchain amino acids. The amino acids can be ranked in the following order with respect to

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010904, Length=362, Percent_Identity=35.6353591160221, Blast_Score=221, Evalue=9e-58,
Organism=Homo sapiens, GI296010906, Length=362, Percent_Identity=35.6353591160221, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI38176287, Length=362, Percent_Identity=35.6353591160221, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI50658084, Length=352, Percent_Identity=34.0909090909091, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI296010902, Length=362, Percent_Identity=30.939226519337, Blast_Score=175, Evalue=5e-44,
Organism=Homo sapiens, GI296010900, Length=362, Percent_Identity=30.939226519337, Blast_Score=175, Evalue=6e-44,
Organism=Homo sapiens, GI258614015, Length=294, Percent_Identity=34.3537414965986, Blast_Score=167, Evalue=2e-41,
Organism=Escherichia coli, GI48994963, Length=294, Percent_Identity=29.9319727891156, Blast_Score=115, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17568601, Length=347, Percent_Identity=33.1412103746398, Blast_Score=203, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17565728, Length=343, Percent_Identity=31.4868804664723, Blast_Score=169, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6322608, Length=342, Percent_Identity=35.9649122807018, Blast_Score=212, Evalue=6e-56,
Organism=Saccharomyces cerevisiae, GI6322002, Length=348, Percent_Identity=35.9195402298851, Blast_Score=209, Evalue=5e-55,
Organism=Drosophila melanogaster, GI24641779, Length=331, Percent_Identity=36.2537764350453, Blast_Score=205, Evalue=4e-53,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 40059; Mature: 39928

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKNYKKTLLVNPISQDELQKRLSAPGFGSVFSEHMVSADWHRDLGWHDYRVEPYGPIS
CCCCCCCCEEEECCCCHHHHHHHHCCCCCHHHHHHHHHCCCHHCCCCCCCEECCCCCCCC
LAPSAMVFHYGQEIFEGLKIFRHSQDSSVRAFRPLENAKRFQKSSERIALPVLPVEDFLQ
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHCCCEEEEEEECHHHHHH
SLREWVMVDGRWVPNAPSCVYARPFMIATEEALGLRATCSARYTLICCPVGDLFGQIRPI
HHHHHHEECCEECCCCCCEEEECCEEEEEHHHCCEEEECCCCEEEEEECHHHHHCCEEEE
KVRVSSKYSRASRGGTGSVKFGGNYAASILPQVEAGNNGFDQVLFLDSASYKNIEELSAS
EEEECCCHHHHCCCCCCEEEECCCCHHHHCEEECCCCCCCCEEEEEECCCCCCHHHHCCC
NIFFVLSSKTLITPSLDGTILPGVTRDSIIRIAEDLGFVVEQRQISLDEVTHLFSQDKVT
CEEEEEECCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHH
EMFCCGTAASVTPIGSVSFDGGTLGSGQAGPVTMLLREYLRDIQFGNRPDKYNWMVNLED
HHEECCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECC
T
C
>Mature Secondary Structure 
SAKNYKKTLLVNPISQDELQKRLSAPGFGSVFSEHMVSADWHRDLGWHDYRVEPYGPIS
CCCCCCCEEEECCCCHHHHHHHHCCCCCHHHHHHHHHCCCHHCCCCCCCEECCCCCCCC
LAPSAMVFHYGQEIFEGLKIFRHSQDSSVRAFRPLENAKRFQKSSERIALPVLPVEDFLQ
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHCCCEEEEEEECHHHHHH
SLREWVMVDGRWVPNAPSCVYARPFMIATEEALGLRATCSARYTLICCPVGDLFGQIRPI
HHHHHHEECCEECCCCCCEEEECCEEEEEHHHCCEEEECCCCEEEEEECHHHHHCCEEEE
KVRVSSKYSRASRGGTGSVKFGGNYAASILPQVEAGNNGFDQVLFLDSASYKNIEELSAS
EEEECCCHHHHCCCCCCEEEECCCCHHHHCEEECCCCCCCCEEEEEECCCCCCHHHHCCC
NIFFVLSSKTLITPSLDGTILPGVTRDSIIRIAEDLGFVVEQRQISLDEVTHLFSQDKVT
CEEEEEECCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHH
EMFCCGTAASVTPIGSVSFDGGTLGSGQAGPVTMLLREYLRDIQFGNRPDKYNWMVNLED
HHEECCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECC
T
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]