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The map label for this gene is gltX

Identifier: 28572711

GI number: 28572711

Start: 600620

End: 602062

Strand: Reverse

Name: gltX

Synonym: TW563

Alternate gene names: 28572711

Gene position: 602062-600620 (Counterclockwise)

Preceding gene: 28572712

Following gene: 28572710

Centisome position: 65.02

GC content: 44.63

Gene sequence:

>1443_bases
GTGCGTGACCCGCGTGTGCGGGTTAGATTTTGCCCGTCTCCAACTGGTGCACCCCACCTGGGCCTTGTAAGAACTGCGCT
TTTTAACTGGGTGTTTGCACGAAAACATGGTGGGGGTTTCATTTTTCGTATAGAGGATACGGATGCGACGAGAAATAGAG
AGGAGAGTTGTTCGCAGCTGATTGATACCCTGAAGTGGCTGGGTCTTGATTGGGACGAGGGTCCAGATAAGGGCGGTCAG
TTTGGTCCATATTATCAGTCACAGAGGGGTGATATATATCAGGAAGTGCTCGATAAGCTCCTTGCGGCAGATCTTGCGTA
TGAAAGTTTTTCGACCAAAGAAGAAATAGAGAAGCGTAATCTAGAGGCCGGCCGTCCGATTCAATTGGGATATGACAATT
ACGACAGGACTCTGTCAGAACAAACCAAGGCCGCAATGCGCGAGGCCGGTCGTACGCCAATAATAAGACTGCGCATGGAT
GATGAGAATATTGCGTTTGAGGATCTTGTAAAGGGTGAAGTGGTATTCACAGATCCCATTCCGGATTTTGCACTTACAAG
AGCTTCTGGTGAGCCACTCTACACACTTGTAAACCCCGTTGACGATGCATTTATGAAGATCACTCATGTCCTGCGGGGCG
AGGATTTGTTGTCATCAACTCCAAGACAAATTGCCCTGTATAAGGCACTTATAACAATAGGTATTACAGATTACGTTCCG
TTTTTTGGACATTTGCCAATTGTTATGGGTGAGGGTAACAGAAAATTATCTAAACGAAATCCTGAGTCAGATTTTTATTT
CTATAAAGCACGTGGATTTATTCGTGAAGGTCTTTTGAATTATCTGTCGTTACTGGGCTGGTCAATTAGTAATAGCAGGG
ATACATTTTCTTTATCTGAGATGATACATGCTTTTGACGTGCGGGACGTAAGGGGTAATCCGGCGCGTTTTGATTATAAG
AAGTGCCTTGCAATCAATGCCTATCACCTGCGTGAGCTTAATGTGGAAGATTTTTTTCTTCGACTTGTTCCATTTGTTGA
AGAGATGCTCGGTATTCCACTTTCTTTTGAGCAGAAAAATAGCCTTCGGGCAATATGTCCATTTGTGCAGGGGAGGGTTC
AGTTGTTGACCGAAGCGGCAGAGATGGTTAGATTCCTATTGGTTGATAATATCTCTGTTGATTTTGCCGTAACGGATAAA
GAAATTGATGTGCTGCGCCACTGTCTAGCGCTGCTGAATTTGCTTGATACGTGGGAAAGTGCACAAATTGCCTCTTGTAT
AAAACAGGCAATTGAACAGTTTGATCTACAGCCGAAACGCATATTCTCGATTTTAAGGCTTGCAATTACCGGTCGCAGAG
TCTCTCCGCCGCTTTTTGAGTCGATGCAGATTCTGGGCAGATCGGCAAGCCTTAACCGTGTTGAAACTTTTGTAACAAAC
TAA

Upstream 100 bases:

>100_bases
TGGTAATCGTCCGGATAAATACAACTGGATGGTCAATCTTGAGGATACTTAGGTGAGTTTTTGGTCTGTAAGAAACACTT
GAACAGACGGGGGTGTTTTT

Downstream 100 bases:

>100_bases
GTGTGTGTCGGTGTGATTGATGTCGCAATTATTGGCGGGGGTCCTGCTGGACTTACCGCAGGTATAAGCCTTGCCCGAAT
GGGGTGGAAGGTCTGTGTTT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MRDPRVRVRFCPSPTGAPHLGLVRTALFNWVFARKHGGGFIFRIEDTDATRNREESCSQLIDTLKWLGLDWDEGPDKGGQ
FGPYYQSQRGDIYQEVLDKLLAADLAYESFSTKEEIEKRNLEAGRPIQLGYDNYDRTLSEQTKAAMREAGRTPIIRLRMD
DENIAFEDLVKGEVVFTDPIPDFALTRASGEPLYTLVNPVDDAFMKITHVLRGEDLLSSTPRQIALYKALITIGITDYVP
FFGHLPIVMGEGNRKLSKRNPESDFYFYKARGFIREGLLNYLSLLGWSISNSRDTFSLSEMIHAFDVRDVRGNPARFDYK
KCLAINAYHLRELNVEDFFLRLVPFVEEMLGIPLSFEQKNSLRAICPFVQGRVQLLTEAAEMVRFLLVDNISVDFAVTDK
EIDVLRHCLALLNLLDTWESAQIASCIKQAIEQFDLQPKRIFSILRLAITGRRVSPPLFESMQILGRSASLNRVETFVTN

Sequences:

>Translated_480_residues
MRDPRVRVRFCPSPTGAPHLGLVRTALFNWVFARKHGGGFIFRIEDTDATRNREESCSQLIDTLKWLGLDWDEGPDKGGQ
FGPYYQSQRGDIYQEVLDKLLAADLAYESFSTKEEIEKRNLEAGRPIQLGYDNYDRTLSEQTKAAMREAGRTPIIRLRMD
DENIAFEDLVKGEVVFTDPIPDFALTRASGEPLYTLVNPVDDAFMKITHVLRGEDLLSSTPRQIALYKALITIGITDYVP
FFGHLPIVMGEGNRKLSKRNPESDFYFYKARGFIREGLLNYLSLLGWSISNSRDTFSLSEMIHAFDVRDVRGNPARFDYK
KCLAINAYHLRELNVEDFFLRLVPFVEEMLGIPLSFEQKNSLRAICPFVQGRVQLLTEAAEMVRFLLVDNISVDFAVTDK
EIDVLRHCLALLNLLDTWESAQIASCIKQAIEQFDLQPKRIFSILRLAITGRRVSPPLFESMQILGRSASLNRVETFVTN
>Mature_480_residues
MRDPRVRVRFCPSPTGAPHLGLVRTALFNWVFARKHGGGFIFRIEDTDATRNREESCSQLIDTLKWLGLDWDEGPDKGGQ
FGPYYQSQRGDIYQEVLDKLLAADLAYESFSTKEEIEKRNLEAGRPIQLGYDNYDRTLSEQTKAAMREAGRTPIIRLRMD
DENIAFEDLVKGEVVFTDPIPDFALTRASGEPLYTLVNPVDDAFMKITHVLRGEDLLSSTPRQIALYKALITIGITDYVP
FFGHLPIVMGEGNRKLSKRNPESDFYFYKARGFIREGLLNYLSLLGWSISNSRDTFSLSEMIHAFDVRDVRGNPARFDYK
KCLAINAYHLRELNVEDFFLRLVPFVEEMLGIPLSFEQKNSLRAICPFVQGRVQLLTEAAEMVRFLLVDNISVDFAVTDK
EIDVLRHCLALLNLLDTWESAQIASCIKQAIEQFDLQPKRIFSILRLAITGRRVSPPLFESMQILGRSASLNRVETFVTN

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=500, Percent_Identity=32.2, Blast_Score=218, Evalue=9e-57,
Organism=Escherichia coli, GI1788743, Length=483, Percent_Identity=31.4699792960663, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI226510925, Length=306, Percent_Identity=29.7385620915033, Blast_Score=110, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71993394, Length=478, Percent_Identity=30.7531380753138, Blast_Score=205, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6324540, Length=495, Percent_Identity=29.8989898989899, Blast_Score=185, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24665321, Length=487, Percent_Identity=31.6221765913758, Blast_Score=228, Evalue=7e-60,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_TROW8 (Q83HJ1)

Other databases:

- EMBL:   BX251411
- RefSeq:   NP_789491.1
- ProteinModelPortal:   Q83HJ1
- SMR:   Q83HJ1
- STRING:   Q83HJ1
- GeneID:   1064766
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW563
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- ProtClustDB:   PRK01406
- BioCyc:   TWHI218496:TW0536-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 54801; Mature: 54801

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 258-258

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDPRVRVRFCPSPTGAPHLGLVRTALFNWVFARKHGGGFIFRIEDTDATRNREESCSQL
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHEECCCEEEEEEECCCCCCCHHHHHHHH
IDTLKWLGLDWDEGPDKGGQFGPYYQSQRGDIYQEVLDKLLAADLAYESFSTKEEIEKRN
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHC
LEAGRPIQLGYDNYDRTLSEQTKAAMREAGRTPIIRLRMDDENIAFEDLVKGEVVFTDPI
CCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHCCCEEEECCC
PDFALTRASGEPLYTLVNPVDDAFMKITHVLRGEDLLSSTPRQIALYKALITIGITDYVP
CCHHEECCCCCCEEEECCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHH
FFGHLPIVMGEGNRKLSKRNPESDFYFYKARGFIREGLLNYLSLLGWSISNSRDTFSLSE
HHCCCCEEECCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
MIHAFDVRDVRGNPARFDYKKCLAINAYHLRELNVEDFFLRLVPFVEEMLGIPLSFEQKN
HHHHHCCCCCCCCCCCCCHHHHHEECHHHHEECCHHHHHHHHHHHHHHHHCCCCCCCCCC
SLRAICPFVQGRVQLLTEAAEMVRFLLVDNISVDFAVTDKEIDVLRHCLALLNLLDTWES
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHCCCC
AQIASCIKQAIEQFDLQPKRIFSILRLAITGRRVSPPLFESMQILGRSASLNRVETFVTN
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCC
>Mature Secondary Structure
MRDPRVRVRFCPSPTGAPHLGLVRTALFNWVFARKHGGGFIFRIEDTDATRNREESCSQL
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHEECCCEEEEEEECCCCCCCHHHHHHHH
IDTLKWLGLDWDEGPDKGGQFGPYYQSQRGDIYQEVLDKLLAADLAYESFSTKEEIEKRN
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHC
LEAGRPIQLGYDNYDRTLSEQTKAAMREAGRTPIIRLRMDDENIAFEDLVKGEVVFTDPI
CCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHCCCEEEECCC
PDFALTRASGEPLYTLVNPVDDAFMKITHVLRGEDLLSSTPRQIALYKALITIGITDYVP
CCHHEECCCCCCEEEECCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHH
FFGHLPIVMGEGNRKLSKRNPESDFYFYKARGFIREGLLNYLSLLGWSISNSRDTFSLSE
HHCCCCEEECCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
MIHAFDVRDVRGNPARFDYKKCLAINAYHLRELNVEDFFLRLVPFVEEMLGIPLSFEQKN
HHHHHCCCCCCCCCCCCCHHHHHEECHHHHEECCHHHHHHHHHHHHHHHHCCCCCCCCCC
SLRAICPFVQGRVQLLTEAAEMVRFLLVDNISVDFAVTDKEIDVLRHCLALLNLLDTWES
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHCCCC
AQIASCIKQAIEQFDLQPKRIFSILRLAITGRRVSPPLFESMQILGRSASLNRVETFVTN
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12606174