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The map label for this gene is map [H]

Identifier: 28572660

GI number: 28572660

Start: 549738

End: 550607

Strand: Direct

Name: map [H]

Synonym: TW511

Alternate gene names: 28572660

Gene position: 549738-550607 (Clockwise)

Preceding gene: 28572659

Following gene: 28572664

Centisome position: 59.37

GC content: 44.94

Gene sequence:

>870_bases
ATGACCCCAAAGAATGCATATGGCCATCTTGTGCCGGGAGATATTTCCGCCGCAAGATGGGTTGATCCCAAAATTGCGCG
GCCCAATTATGTTGGAAAAAAAAGAGTCCCAAGGTATACAGGTTCGGATATTTATAACAAAGATGAAATTGAACGAGTGA
GGGCTGCCGGGTCAATAGCAAGCCAAACATTGGATTATCTCGCAGAGCATATACGGGCAGGAATTACAACAGACGAACTC
GATAGACTTGCGCATGAGTTTATAACCTCAAAAGGCGCGTACCCATCACCATTGGGCTATCAAGGCTTTCCAAAGTCTAT
CTGCACTTCAATAAACGAAGTGATTTGCCACGGAATACCGGATAACACTGTTTTGGAAGATTGCGACATTGTGAATGTAG
ATGTAACAGCATTTTTTGAGGGAATGCACGGTGATACAAACAGGACATTTATCATCGGAAATGCTCCTGAAGCAACCAAA
AATCTCGTGAAAAATACAGAAGAAGCCCTGCATGTGGCAATAAAGGCGGTTGCACCCGGGAGAAGGGTCAATGTAATAGG
GCTTACAATAGAGCGGCTTGCAAAGCGCTTTAACTACGGTGTGGTAAGAGAATTCACAGGCCACGGTGTTGGAAGGGCAT
TTCATACTGGACTTGTTATACCCCACTATGATGCAACACCGTATTATGATCGCATTCTTAAGCCCGGCATGATATTTACC
ATAGAGCCCATGCTGAACCTCGGTACAAGGCATTGGAATATGTGGGAAGATGGGTGGACTGCAGTTACAAAAGATCTTTT
ACCTTCCGCACAGTTCGAGCACACAATTGTTGTTACGCAAAATGGGGCGGAAATACTCACCCTGTCATAG

Upstream 100 bases:

>100_bases
GATACGCAAGTATTGATAATACTGTGGCATCCTGCGTAAAACTATGGCGAAAAGATGTTCTAGAGGGCAATTTCCCTCAG
AAAGACCATATTCCAGCATA

Downstream 100 bases:

>100_bases
GCACCAAAACACTATATAACTTAGAACTAACTAGAAAAATATATGTGAATATACATTACGCCATAGGCGGACACCATCCT
GCAGTATTGAATGCTAGGGC

Product: methionine aminopeptidase

Products: NA

Alternate protein names: MAP; Peptidase M [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MTPKNAYGHLVPGDISAARWVDPKIARPNYVGKKRVPRYTGSDIYNKDEIERVRAAGSIASQTLDYLAEHIRAGITTDEL
DRLAHEFITSKGAYPSPLGYQGFPKSICTSINEVICHGIPDNTVLEDCDIVNVDVTAFFEGMHGDTNRTFIIGNAPEATK
NLVKNTEEALHVAIKAVAPGRRVNVIGLTIERLAKRFNYGVVREFTGHGVGRAFHTGLVIPHYDATPYYDRILKPGMIFT
IEPMLNLGTRHWNMWEDGWTAVTKDLLPSAQFEHTIVVTQNGAEILTLS

Sequences:

>Translated_289_residues
MTPKNAYGHLVPGDISAARWVDPKIARPNYVGKKRVPRYTGSDIYNKDEIERVRAAGSIASQTLDYLAEHIRAGITTDEL
DRLAHEFITSKGAYPSPLGYQGFPKSICTSINEVICHGIPDNTVLEDCDIVNVDVTAFFEGMHGDTNRTFIIGNAPEATK
NLVKNTEEALHVAIKAVAPGRRVNVIGLTIERLAKRFNYGVVREFTGHGVGRAFHTGLVIPHYDATPYYDRILKPGMIFT
IEPMLNLGTRHWNMWEDGWTAVTKDLLPSAQFEHTIVVTQNGAEILTLS
>Mature_288_residues
TPKNAYGHLVPGDISAARWVDPKIARPNYVGKKRVPRYTGSDIYNKDEIERVRAAGSIASQTLDYLAEHIRAGITTDELD
RLAHEFITSKGAYPSPLGYQGFPKSICTSINEVICHGIPDNTVLEDCDIVNVDVTAFFEGMHGDTNRTFIIGNAPEATKN
LVKNTEEALHVAIKAVAPGRRVNVIGLTIERLAKRFNYGVVREFTGHGVGRAFHTGLVIPHYDATPYYDRILKPGMIFTI
EPMLNLGTRHWNMWEDGWTAVTKDLLPSAQFEHTIVVTQNGAEILTLS

Specific function: Removes the amino-terminal methionine from nascent proteins [H]

COG id: COG0024

COG function: function code J; Methionine aminopeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24A family [H]

Homologues:

Organism=Homo sapiens, GI164420681, Length=244, Percent_Identity=45.9016393442623, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI40385867, Length=279, Percent_Identity=41.9354838709677, Blast_Score=225, Evalue=3e-59,
Organism=Escherichia coli, GI1786364, Length=247, Percent_Identity=46.1538461538462, Blast_Score=237, Evalue=6e-64,
Organism=Escherichia coli, GI1788728, Length=250, Percent_Identity=24, Blast_Score=69, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71996291, Length=289, Percent_Identity=44.2906574394464, Blast_Score=233, Evalue=9e-62,
Organism=Saccharomyces cerevisiae, GI6323273, Length=282, Percent_Identity=45.7446808510638, Blast_Score=253, Evalue=2e-68,
Organism=Drosophila melanogaster, GI21355531, Length=287, Percent_Identity=45.2961672473868, Blast_Score=248, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24583427, Length=280, Percent_Identity=44.2857142857143, Blast_Score=227, Evalue=7e-60,

Paralogues:

None

Copy number: 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR002467 [H]

Pfam domain/function: PF00557 Peptidase_M24 [H]

EC number: =3.4.11.18 [H]

Molecular weight: Translated: 32059; Mature: 31928

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00680 MAP_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPKNAYGHLVPGDISAARWVDPKIARPNYVGKKRVPRYTGSDIYNKDEIERVRAAGSIA
CCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
SQTLDYLAEHIRAGITTDELDRLAHEFITSKGAYPSPLGYQGFPKSICTSINEVICHGIP
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
DNTVLEDCDIVNVDVTAFFEGMHGDTNRTFIIGNAPEATKNLVKNTEEALHVAIKAVAPG
CCCEECCCCEEEEEHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCC
RRVNVIGLTIERLAKRFNYGVVREFTGHGVGRAFHTGLVIPHYDATPYYDRILKPGMIFT
CEEEEEEHHHHHHHHHCCCCCEEHHCCCCCCHHHHCCEEEECCCCCHHHHHHCCCCEEEE
IEPMLNLGTRHWNMWEDGWTAVTKDLLPSAQFEHTIVVTQNGAEILTLS
ECHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEC
>Mature Secondary Structure 
TPKNAYGHLVPGDISAARWVDPKIARPNYVGKKRVPRYTGSDIYNKDEIERVRAAGSIA
CCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
SQTLDYLAEHIRAGITTDELDRLAHEFITSKGAYPSPLGYQGFPKSICTSINEVICHGIP
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
DNTVLEDCDIVNVDVTAFFEGMHGDTNRTFIIGNAPEATKNLVKNTEEALHVAIKAVAPG
CCCEECCCCEEEEEHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCC
RRVNVIGLTIERLAKRFNYGVVREFTGHGVGRAFHTGLVIPHYDATPYYDRILKPGMIFT
CEEEEEEHHHHHHHHHCCCCCEEHHCCCCCCHHHHCCEEEECCCCCHHHHHHCCCCEEEE
IEPMLNLGTRHWNMWEDGWTAVTKDLLPSAQFEHTIVVTQNGAEILTLS
ECHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]