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The map label for this gene is panB [H]

Identifier: 28572659

GI number: 28572659

Start: 548929

End: 549738

Strand: Direct

Name: panB [H]

Synonym: TW510

Alternate gene names: 28572659

Gene position: 548929-549738 (Clockwise)

Preceding gene: 28572658

Following gene: 28572660

Centisome position: 59.28

GC content: 44.69

Gene sequence:

>810_bases
ATGTCACGCAAACCTTCTCCAACCAGACGAACAAGGATACATCGCTTTCATAAAGGTTCCTGCGGCAGGAAGCTGGTGGG
TCTTACATGTTATGACTTTTCAACAGCCAGGGTCCTGAGTGATTGCGAGCTCGACTTTCTTCTAGTTGGTGATTCAGCAA
GCGGTGTGATTTATGGATATGAAAACACAGGCAGTGTCTGTCTTGACGAAATTATTTACCTTGCCGCAGGCGTTGTAAGA
GGTGCACCAAATAGCTTTATTATTGTTGACCTTCCCTTTGGAACATACGAAAAATCTGATGAATTAGCTGTCGAAACAGC
AATAGAGGTTATAAAAAGAACTGGGGCCTCGGCTGTAAAGCTTGAAGGTGGTGCTCGAATGGCATGTAGGATAAGCGCGA
TTGTAAGAGCTGGTGTGCCGGTTATGGGACATATTGGGTTTACCCCGCAGACCATTAACGCTCTTGGGGGGTACAAAATA
CAGGGACGTGATAACGCCGATTTAATTTACCTGGATGCACAGGCTGTTGAGCAGGCAGGGGCTTTTGCGGTTGTTATGGA
AATGGTAACTGAAGATCTTGCGAAAACCATAACCTCAGAAATCAAAATAGCAACAATTGGCGTCGGCGCGGGGAGATACA
CAGATGGACAGCTATTAGTCATAAATGATTTAATTGGCCTGAGTGAAAAGAAAATAACATTCGCGCCACGATACGCAAGT
ATTGATAATACTGTGGCATCCTGCGTAAAACTATGGCGAAAAGATGTTCTAGAGGGCAATTTCCCTCAGAAAGACCATAT
TCCAGCATAA

Upstream 100 bases:

>100_bases
TTAGCGCATGGATTGATTACAAGAGGAAGAATGAGCTTAACCGGATTGCGCAGTATCCTCATCCACTTGAATTTGGGATG
TATTATGGGGTGTGATCTGT

Downstream 100 bases:

>100_bases
TGACCCCAAAGAATGCATATGGCCATCTTGTGCCGGGAGATATTTCCGCCGCAAGATGGGTTGATCCCAAAATTGCGCGG
CCCAATTATGTTGGAAAAAA

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT [H]

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MSRKPSPTRRTRIHRFHKGSCGRKLVGLTCYDFSTARVLSDCELDFLLVGDSASGVIYGYENTGSVCLDEIIYLAAGVVR
GAPNSFIIVDLPFGTYEKSDELAVETAIEVIKRTGASAVKLEGGARMACRISAIVRAGVPVMGHIGFTPQTINALGGYKI
QGRDNADLIYLDAQAVEQAGAFAVVMEMVTEDLAKTITSEIKIATIGVGAGRYTDGQLLVINDLIGLSEKKITFAPRYAS
IDNTVASCVKLWRKDVLEGNFPQKDHIPA

Sequences:

>Translated_269_residues
MSRKPSPTRRTRIHRFHKGSCGRKLVGLTCYDFSTARVLSDCELDFLLVGDSASGVIYGYENTGSVCLDEIIYLAAGVVR
GAPNSFIIVDLPFGTYEKSDELAVETAIEVIKRTGASAVKLEGGARMACRISAIVRAGVPVMGHIGFTPQTINALGGYKI
QGRDNADLIYLDAQAVEQAGAFAVVMEMVTEDLAKTITSEIKIATIGVGAGRYTDGQLLVINDLIGLSEKKITFAPRYAS
IDNTVASCVKLWRKDVLEGNFPQKDHIPA
>Mature_268_residues
SRKPSPTRRTRIHRFHKGSCGRKLVGLTCYDFSTARVLSDCELDFLLVGDSASGVIYGYENTGSVCLDEIIYLAAGVVRG
APNSFIIVDLPFGTYEKSDELAVETAIEVIKRTGASAVKLEGGARMACRISAIVRAGVPVMGHIGFTPQTINALGGYKIQ
GRDNADLIYLDAQAVEQAGAFAVVMEMVTEDLAKTITSEIKIATIGVGAGRYTDGQLLVINDLIGLSEKKITFAPRYASI
DNTVASCVKLWRKDVLEGNFPQKDHIPA

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate [H]

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family [H]

Homologues:

Organism=Escherichia coli, GI1786326, Length=263, Percent_Identity=37.6425855513308, Blast_Score=176, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6319653, Length=253, Percent_Identity=34.3873517786561, Blast_Score=152, Evalue=5e-38,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003700
- InterPro:   IPR015813 [H]

Pfam domain/function: PF02548 Pantoate_transf [H]

EC number: =2.1.2.11 [H]

Molecular weight: Translated: 28979; Mature: 28848

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKPSPTRRTRIHRFHKGSCGRKLVGLTCYDFSTARVLSDCELDFLLVGDSASGVIYGY
CCCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHCCCEEEEEECCCCCEEEEEE
ENTGSVCLDEIIYLAAGVVRGAPNSFIIVDLPFGTYEKSDELAVETAIEVIKRTGASAVK
CCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEE
LEGGARMACRISAIVRAGVPVMGHIGFTPQTINALGGYKIQGRDNADLIYLDAQAVEQAG
ECCCCEEEHHHHHHHHCCCCEEEECCCCHHHHHHCCCEEEECCCCCCEEEEEHHHHHHCC
AFAVVMEMVTEDLAKTITSEIKIATIGVGAGRYTDGQLLVINDLIGLSEKKITFAPRYAS
HHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCEEEEEECCCCCCCCEEEECCCCCC
IDNTVASCVKLWRKDVLEGNFPQKDHIPA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SRKPSPTRRTRIHRFHKGSCGRKLVGLTCYDFSTARVLSDCELDFLLVGDSASGVIYGY
CCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHCCCEEEEEECCCCCEEEEEE
ENTGSVCLDEIIYLAAGVVRGAPNSFIIVDLPFGTYEKSDELAVETAIEVIKRTGASAVK
CCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEE
LEGGARMACRISAIVRAGVPVMGHIGFTPQTINALGGYKIQGRDNADLIYLDAQAVEQAG
ECCCCEEEHHHHHHHHCCCCEEEECCCCHHHHHHCCCEEEECCCCCCEEEEEHHHHHHCC
AFAVVMEMVTEDLAKTITSEIKIATIGVGAGRYTDGQLLVINDLIGLSEKKITFAPRYAS
HHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCEEEEEECCCCCCCCEEEECCCCCC
IDNTVASCVKLWRKDVLEGNFPQKDHIPA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12902375 [H]