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The map label for this gene is menE [H]

Identifier: 28572286

GI number: 28572286

Start: 129025

End: 130161

Strand: Direct

Name: menE [H]

Synonym: TW121

Alternate gene names: 28572286

Gene position: 129025-130161 (Clockwise)

Preceding gene: 28572285

Following gene: 28572287

Centisome position: 13.93

GC content: 46.7

Gene sequence:

>1137_bases
ATGACCAAGGCTCTCATGCGGGGGCAAATTACACTTGACTCTGTAAAAAGAGTCCTTGAGGACGAGCGATTTGCTATTTG
TGCGTCAGAAGAGCCTCTCTCATGCGGGGGCAAAATAATGGTCGACGATGCGGTTTGTCTCGTAATAAATACATCTGGAT
CAACCGCCTCGCCCAAGAGAGTTGCGTTTTCATCATCATCTCTTATGAACTCTGCTCGTGCGTGCTGCTCCGTTCTTGGC
TATGGACAGTGGGTTTTGTGCTTACCAAAGCAATATACTGCCGGGGTGCGTGTGCTTGTACGATCCATTCTGTCTGGCAC
GAAACCAATAGATATTTCTGAGGATAGATTTTCCCCTGAAAATTTCATACATGCATGCCGGAGGTTATCTTGCTCGAATA
CCTTTACTGCACTTGTCCCAACCCAGCTCAGTGCCCTTGTTAGGGTGGCAGAGGATGACAAAAACGTTGCAAAAGTTTTG
AGTGGCTTTACGGGCGTTATTGTCGGAGGGCAGCATATTGCGCGATCACTGCTTGAAAGAAGCAGGTTGCTCGGGATCAA
TTTATTTACTTCATACGGGTTGACCGAAACTTTTGGTGGATGCGTTTACAATGGCCTTCCTCTTCCTGGAGTGCAGATAC
AGATTATTGACGGAAGGGCCGCAATCGCCTCCCCCAGTCTGGCGCTTGGGTATCTAAAAGAAAACGGGCAAATCGATGAA
ACAGGATTTTTTTTTGACCGAGGTGAGCGGTGGTTTCACACAAATGATCTTGCCGTTATGAATCGGGGAAAATTGATAAT
CCACGGCCGGATCGACAGAGTTATAAACAGTGGAGGGATGAAGCTTGACCTAAACGCTATCGAAACAGCTCTTAGATCTC
TTGACTGCGTTGAGGATGCTGTTTTGCTGGGTGTTAAGTCGAAAAAATGGGGTCAGCACTTCTGTGCGTTTATCGATATT
GGTGATGTCACAAAGGCCGATAGGACTGATTGCCTGCAAAAAATAAACACCCTCCTTTCAACCTTTGGCCGCGCGTGCCG
ATCTGGACAGATAAAGCTTATTCATCCAATACCCCGCACTTTTAGCGGTAAACCAGATCGAATTGCCCTACAAAGAGGAT
TACAGAACAATATTTGA

Upstream 100 bases:

>100_bases
ACACGCCTTGCGTATGCAACAGATGAGTCAAAAGAAGGGCGTGACGCATTCCTCGAAAAACGTCGGCCAGACTGGTCAAG
CTTTCCCTGGAGCTTCTAGT

Downstream 100 bases:

>100_bases
GCAGAACAATATTTGCAAGGCCCAAATACCCGATCATCTCAGTGTGGGATGTTAGGCCATATCCATAATGCAACTACTGC
TAGTACTTTTTATGGGGAAG

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MTKALMRGQITLDSVKRVLEDERFAICASEEPLSCGGKIMVDDAVCLVINTSGSTASPKRVAFSSSSLMNSARACCSVLG
YGQWVLCLPKQYTAGVRVLVRSILSGTKPIDISEDRFSPENFIHACRRLSCSNTFTALVPTQLSALVRVAEDDKNVAKVL
SGFTGVIVGGQHIARSLLERSRLLGINLFTSYGLTETFGGCVYNGLPLPGVQIQIIDGRAAIASPSLALGYLKENGQIDE
TGFFFDRGERWFHTNDLAVMNRGKLIIHGRIDRVINSGGMKLDLNAIETALRSLDCVEDAVLLGVKSKKWGQHFCAFIDI
GDVTKADRTDCLQKINTLLSTFGRACRSGQIKLIHPIPRTFSGKPDRIALQRGLQNNI

Sequences:

>Translated_378_residues
MTKALMRGQITLDSVKRVLEDERFAICASEEPLSCGGKIMVDDAVCLVINTSGSTASPKRVAFSSSSLMNSARACCSVLG
YGQWVLCLPKQYTAGVRVLVRSILSGTKPIDISEDRFSPENFIHACRRLSCSNTFTALVPTQLSALVRVAEDDKNVAKVL
SGFTGVIVGGQHIARSLLERSRLLGINLFTSYGLTETFGGCVYNGLPLPGVQIQIIDGRAAIASPSLALGYLKENGQIDE
TGFFFDRGERWFHTNDLAVMNRGKLIIHGRIDRVINSGGMKLDLNAIETALRSLDCVEDAVLLGVKSKKWGQHFCAFIDI
GDVTKADRTDCLQKINTLLSTFGRACRSGQIKLIHPIPRTFSGKPDRIALQRGLQNNI
>Mature_377_residues
TKALMRGQITLDSVKRVLEDERFAICASEEPLSCGGKIMVDDAVCLVINTSGSTASPKRVAFSSSSLMNSARACCSVLGY
GQWVLCLPKQYTAGVRVLVRSILSGTKPIDISEDRFSPENFIHACRRLSCSNTFTALVPTQLSALVRVAEDDKNVAKVLS
GFTGVIVGGQHIARSLLERSRLLGINLFTSYGLTETFGGCVYNGLPLPGVQIQIIDGRAAIASPSLALGYLKENGQIDET
GFFFDRGERWFHTNDLAVMNRGKLIIHGRIDRVINSGGMKLDLNAIETALRSLDCVEDAVLLGVKSKKWGQHFCAFIDIG
DVTKADRTDCLQKINTLLSTFGRACRSGQIKLIHPIPRTFSGKPDRIALQRGLQNNI

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA). May be involved in the biosynthesis of menaquinone [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788595, Length=314, Percent_Identity=27.3885350318471, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI145693145, Length=373, Percent_Identity=24.3967828418231, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17560308, Length=376, Percent_Identity=23.4042553191489, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 41170; Mature: 41039

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKALMRGQITLDSVKRVLEDERFAICASEEPLSCGGKIMVDDAVCLVINTSGSTASPKR
CCCCHHCCCEEHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECEEEEEEECCCCCCCCCE
VAFSSSSLMNSARACCSVLGYGQWVLCLPKQYTAGVRVLVRSILSGTKPIDISEDRFSPE
EEECHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
NFIHACRRLSCSNTFTALVPTQLSALVRVAEDDKNVAKVLSGFTGVIVGGQHIARSLLER
HHHHHHHHCCCCCCEEEEHHHHHHHHHHHHCCCHHHHHHHCCCCEEEECHHHHHHHHHHH
SRLLGINLFTSYGLTETFGGCVYNGLPLPGVQIQIIDGRAAIASPSLALGYLKENGQIDE
HHHEEEEHHHHCCCHHHHCCHHCCCCCCCCEEEEEECCCHHHCCCCCEEEEECCCCCCCC
TGFFFDRGERWFHTNDLAVMNRGKLIIHGRIDRVINSGGMKLDLNAIETALRSLDCVEDA
CCCEEECCCCEEECCCEEEEECCEEEEEECHHHHHCCCCCEEEHHHHHHHHHHHHHHHHH
VLLGVKSKKWGQHFCAFIDIGDVTKADRTDCLQKINTLLSTFGRACRSGQIKLIHPIPRT
HHCCCCCCCHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
FSGKPDRIALQRGLQNNI
CCCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
TKALMRGQITLDSVKRVLEDERFAICASEEPLSCGGKIMVDDAVCLVINTSGSTASPKR
CCCHHCCCEEHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECEEEEEEECCCCCCCCCE
VAFSSSSLMNSARACCSVLGYGQWVLCLPKQYTAGVRVLVRSILSGTKPIDISEDRFSPE
EEECHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
NFIHACRRLSCSNTFTALVPTQLSALVRVAEDDKNVAKVLSGFTGVIVGGQHIARSLLER
HHHHHHHHCCCCCCEEEEHHHHHHHHHHHHCCCHHHHHHHCCCCEEEECHHHHHHHHHHH
SRLLGINLFTSYGLTETFGGCVYNGLPLPGVQIQIIDGRAAIASPSLALGYLKENGQIDE
HHHEEEEHHHHCCCHHHHCCHHCCCCCCCCEEEEEECCCHHHCCCCCEEEEECCCCCCCC
TGFFFDRGERWFHTNDLAVMNRGKLIIHGRIDRVINSGGMKLDLNAIETALRSLDCVEDA
CCCEEECCCCEEECCCEEEEECCEEEEEECHHHHHCCCCCEEEHHHHHHHHHHHHHHHHH
VLLGVKSKKWGQHFCAFIDIGDVTKADRTDCLQKINTLLSTFGRACRSGQIKLIHPIPRT
HHCCCCCCCHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
FSGKPDRIALQRGLQNNI
CCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]