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The map label for this gene is purK [H]

Identifier: 28572203

GI number: 28572203

Start: 26979

End: 28232

Strand: Direct

Name: purK [H]

Synonym: TW028

Alternate gene names: 28572203

Gene position: 26979-28232 (Clockwise)

Preceding gene: 28572201

Following gene: 28572204

Centisome position: 2.91

GC content: 48.96

Gene sequence:

>1254_bases
ATGTTATCTATTGCACGCATTGCTTCTCCTGCACAGTATAACCTTAGATTGATGTTCGAGAGATTGAGTTGTTCGGTTGG
TGTGATTGGTGGAGGTCAACTTGCCAGAATGATGATTGCCCCTGCGCAGGCTCTCGGGGTTGATTTAAAGGTCTTTGCAG
ATACCCCTGACAGTTCAGCAGCCCTTGCCGCAACGGCTTTTGGCAGTCCTGAAAATGGTGCGGCAGTCCTCGAATTTGCA
CAGACAGTTGATATTGTGACTTTTGAGCACGAGCTTGTCCCGGCTGATGTTCTGCAACTCTTGGATGAGAGGGGTATTGA
GATGCTGCCTTGCCCGCGCGCCTTGCGGTATGCTCAAGATAAATTGGCATTGCGACGCTATCTCGATGAGATTTCAGTAA
ATCAGCCTGCCTGGGCCGAGGTACGCTCGGAAGGGGATTTAGAACGTTTTATTTCTGAACATGGTGGTTGTGCTGTTGTA
AAAACCGGAGGGTATGACGGACGGGGTGTCCAGGTTGTCACGCTGAGCTCTGAGATTGATACTTGCCGCAACGGAGGTTT
TCTTGCCGAGGAAAGAATTGATTTTGTCTGCGAAGTATCTCAATTAGTTGCCCGCTCATCCATCGGCGAAATCTGCGTAT
GGCCGCTTACACAAACAATTCAGGAGAACGGCATATGTGTTCAGACGATAACGCCAGCACTTGGCCTATCGGGCGGGAAT
GCGCTAAATCAACTCACCACCTTTCACGATGGCAGAACACGCACCCCACACGCGCCGTTACACTCTGAATTGATTACTAA
ACTCCAGGATTCAGCAAAAACAATCGCCCTTGATATTGCGAGAGGTTTAAATGTTGTTGGCGTTATGGCTGTTGAAATGT
TCATTACGCAGGACGGCAAGCTCCTTGTGAACGAACTTGCAATGCGGCCACATAACAGCGGGCATTGGAGCATGGCCGCA
TCGATTACAGACCAGTTTGAACAACACCTGCGTGCCGTACTCGGCTTGCCGTTGGGTGCAACTGATATGTCTCACAACTG
CGCCATAATGGTGAATATATTCAATTCAATTTCGCCCGCTCAATATAGAGAGGTTATGACGCATTGGCCAGACGCGAAGC
TTCATATATACAAGAAACAGCCAAGGCCGGGTCGCAAAATCGGTCATGTTGTTTTTGCTGGTGAAGATTTACAACAGCTT
GCCATTGCAGCTGAAGAATGCAGGCAATTGCTGAGAGAGTCACATGCGGTGTAA

Upstream 100 bases:

>100_bases
GAAAACTAATATATTGACAAATATCCCGGCTATCCCAACAATGCCAAAGGTTGCAATTTGCCTGTATAGAGCTTTATTCA
CCCGGCAGAATGCCCTTTGT

Downstream 100 bases:

>100_bases
GCAATGCACGTCATGCGTCTCAATGCTGTATACCAATTGCAATATAAGTGACGGTGATACAAATTTGGTGGTTCAAATAT
GACTGGTAAAAACATCAACG

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit PurK

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MLSIARIASPAQYNLRLMFERLSCSVGVIGGGQLARMMIAPAQALGVDLKVFADTPDSSAALAATAFGSPENGAAVLEFA
QTVDIVTFEHELVPADVLQLLDERGIEMLPCPRALRYAQDKLALRRYLDEISVNQPAWAEVRSEGDLERFISEHGGCAVV
KTGGYDGRGVQVVTLSSEIDTCRNGGFLAEERIDFVCEVSQLVARSSIGEICVWPLTQTIQENGICVQTITPALGLSGGN
ALNQLTTFHDGRTRTPHAPLHSELITKLQDSAKTIALDIARGLNVVGVMAVEMFITQDGKLLVNELAMRPHNSGHWSMAA
SITDQFEQHLRAVLGLPLGATDMSHNCAIMVNIFNSISPAQYREVMTHWPDAKLHIYKKQPRPGRKIGHVVFAGEDLQQL
AIAAEECRQLLRESHAV

Sequences:

>Translated_417_residues
MLSIARIASPAQYNLRLMFERLSCSVGVIGGGQLARMMIAPAQALGVDLKVFADTPDSSAALAATAFGSPENGAAVLEFA
QTVDIVTFEHELVPADVLQLLDERGIEMLPCPRALRYAQDKLALRRYLDEISVNQPAWAEVRSEGDLERFISEHGGCAVV
KTGGYDGRGVQVVTLSSEIDTCRNGGFLAEERIDFVCEVSQLVARSSIGEICVWPLTQTIQENGICVQTITPALGLSGGN
ALNQLTTFHDGRTRTPHAPLHSELITKLQDSAKTIALDIARGLNVVGVMAVEMFITQDGKLLVNELAMRPHNSGHWSMAA
SITDQFEQHLRAVLGLPLGATDMSHNCAIMVNIFNSISPAQYREVMTHWPDAKLHIYKKQPRPGRKIGHVVFAGEDLQQL
AIAAEECRQLLRESHAV
>Mature_417_residues
MLSIARIASPAQYNLRLMFERLSCSVGVIGGGQLARMMIAPAQALGVDLKVFADTPDSSAALAATAFGSPENGAAVLEFA
QTVDIVTFEHELVPADVLQLLDERGIEMLPCPRALRYAQDKLALRRYLDEISVNQPAWAEVRSEGDLERFISEHGGCAVV
KTGGYDGRGVQVVTLSSEIDTCRNGGFLAEERIDFVCEVSQLVARSSIGEICVWPLTQTIQENGICVQTITPALGLSGGN
ALNQLTTFHDGRTRTPHAPLHSELITKLQDSAKTIALDIARGLNVVGVMAVEMFITQDGKLLVNELAMRPHNSGHWSMAA
SITDQFEQHLRAVLGLPLGATDMSHNCAIMVNIFNSISPAQYREVMTHWPDAKLHIYKKQPRPGRKIGHVVFAGEDLQQL
AIAAEECRQLLRESHAV

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=402, Percent_Identity=29.3532338308458, Blast_Score=121, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6324702, Length=412, Percent_Identity=29.6116504854369, Blast_Score=157, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 45384; Mature: 45384

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSIARIASPAQYNLRLMFERLSCSVGVIGGGQLARMMIAPAQALGVDLKVFADTPDSSA
CCCHHHHCCCCHHHHHHHEEHHCCCEEEECCHHHHHHHHCCHHHHCCEEEEEECCCCCCC
ALAATAFGSPENGAAVLEFAQTVDIVTFEHELVPADVLQLLDERGIEMLPCPRALRYAQD
EEEEEECCCCCCCHHHHHHHHHHEEEEEECCCCHHHHHHHHHHCCCEECCCCHHHHHHHH
KLALRRYLDEISVNQPAWAEVRSEGDLERFISEHGGCAVVKTGGYDGRGVQVVTLSSEID
HHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCCCEEEEEECCCHH
TCRNGGFLAEERIDFVCEVSQLVARSSIGEICVWPLTQTIQENGICVQTITPALGLSGGN
HHCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHCCEEEEECCHHHCCCCCC
ALNQLTTFHDGRTRTPHAPLHSELITKLQDSAKTIALDIARGLNVVGVMAVEMFITQDGK
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHEEHHHHHCCCHHHHHHHHHHHCCCCH
LLVNELAMRPHNSGHWSMAASITDQFEQHLRAVLGLPLGATDMSHNCAIMVNIFNSISPA
HHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHH
QYREVMTHWPDAKLHIYKKQPRPGRKIGHVVFAGEDLQQLAIAAEECRQLLRESHAV
HHHHHHHHCCCCEEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLSIARIASPAQYNLRLMFERLSCSVGVIGGGQLARMMIAPAQALGVDLKVFADTPDSSA
CCCHHHHCCCCHHHHHHHEEHHCCCEEEECCHHHHHHHHCCHHHHCCEEEEEECCCCCCC
ALAATAFGSPENGAAVLEFAQTVDIVTFEHELVPADVLQLLDERGIEMLPCPRALRYAQD
EEEEEECCCCCCCHHHHHHHHHHEEEEEECCCCHHHHHHHHHHCCCEECCCCHHHHHHHH
KLALRRYLDEISVNQPAWAEVRSEGDLERFISEHGGCAVVKTGGYDGRGVQVVTLSSEID
HHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCCCEEEEEECCCHH
TCRNGGFLAEERIDFVCEVSQLVARSSIGEICVWPLTQTIQENGICVQTITPALGLSGGN
HHCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHCCEEEEECCHHHCCCCCC
ALNQLTTFHDGRTRTPHAPLHSELITKLQDSAKTIALDIARGLNVVGVMAVEMFITQDGK
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHEEHHHHHCCCHHHHHHHHHHHCCCCH
LLVNELAMRPHNSGHWSMAASITDQFEQHLRAVLGLPLGATDMSHNCAIMVNIFNSISPA
HHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHH
QYREVMTHWPDAKLHIYKKQPRPGRKIGHVVFAGEDLQQLAIAAEECRQLLRESHAV
HHHHHHHHCCCCEEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]