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The map label for this gene is birA [H]

Identifier: 28572201

GI number: 28572201

Start: 25738

End: 26484

Strand: Direct

Name: birA [H]

Synonym: TW026

Alternate gene names: 28572201

Gene position: 25738-26484 (Clockwise)

Preceding gene: 28572199

Following gene: 28572203

Centisome position: 2.78

GC content: 44.18

Gene sequence:

>747_bases
ATGTTCCCTTGTGTATTTCTAGACACTGTTGGATCAACAAACCTTTACCTTCTTGAATTGCTAAAAGCCAAGGATCTCGA
TGACTTTTTTGCGGTTTTTACCACGAATCAAACCGCAGGACGTGGTCGCATGGCAAGGTCATGGGAATGCAAGAAAGGGA
TTGCGTTGTCTGTATTTCTGAAGCACAATTTGCCAGGAGCCTATATTAACTGGCTTCCACTTGTTATTGGACTTGCCGTT
GTAAAAGCGGTTAATAAAATGTTAGAAGATGCAACATTGCCGCACAGGGCCGACATAAAATGGCCAAATGATGTTCTGAT
TAATGGAAAAAAACTGTCTGGAATACTAATCGAGACACCTTCTGATGGTAATGGATTAATCGTTGGTATTGGGCTGAATG
TGGCTGTTGCACCTATTGCAACTTCTATTTGCCTAAGGCAAGTAGGACTTCAAGCAAGCCCCAAAGAGGTGGTTTCCGCG
CTTCTGGCCGAAATGCATACAATCTATCAACAGCTCATTGCCCTTGCCGCCAAAGACATATCATTTAAAGATTCAGAATG
CTTTGGTGAAATTATGCGGTTGTGCTCAACGATCAACAAGACTGTTAGGGTGACGGGCACCAAGACATTTGTAGGTGTTG
CGCAAGGGCTTGATTCGCTTGGCAGACTTGTTATTCGTCACAGCGGAGACACTTCGATTGTTTCTGCTGGGGATATCGAG
CACCTGCGCGCTATTGACAGTTTGTAA

Upstream 100 bases:

>100_bases
CTCTAGCCCCCTACTGGATTCGTCAAAATTATCGGTCATCAGGGTACTCTATCCTCGATTCATTTTTTTGTTGGCATTTG
TTTTGTTCTAATATTCTTTC

Downstream 100 bases:

>100_bases
AATTACCAACCCTATCTTCGTCTTTATTTATACATGCCTCATGCGTTTCATCTCATTTACGTTTGGAAAAAACCCAGAAA
TAGTACCCGATGAATCGCAG

Product: protein ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MFPCVFLDTVGSTNLYLLELLKAKDLDDFFAVFTTNQTAGRGRMARSWECKKGIALSVFLKHNLPGAYINWLPLVIGLAV
VKAVNKMLEDATLPHRADIKWPNDVLINGKKLSGILIETPSDGNGLIVGIGLNVAVAPIATSICLRQVGLQASPKEVVSA
LLAEMHTIYQQLIALAAKDISFKDSECFGEIMRLCSTINKTVRVTGTKTFVGVAQGLDSLGRLVIRHSGDTSIVSAGDIE
HLRAIDSL

Sequences:

>Translated_248_residues
MFPCVFLDTVGSTNLYLLELLKAKDLDDFFAVFTTNQTAGRGRMARSWECKKGIALSVFLKHNLPGAYINWLPLVIGLAV
VKAVNKMLEDATLPHRADIKWPNDVLINGKKLSGILIETPSDGNGLIVGIGLNVAVAPIATSICLRQVGLQASPKEVVSA
LLAEMHTIYQQLIALAAKDISFKDSECFGEIMRLCSTINKTVRVTGTKTFVGVAQGLDSLGRLVIRHSGDTSIVSAGDIE
HLRAIDSL
>Mature_248_residues
MFPCVFLDTVGSTNLYLLELLKAKDLDDFFAVFTTNQTAGRGRMARSWECKKGIALSVFLKHNLPGAYINWLPLVIGLAV
VKAVNKMLEDATLPHRADIKWPNDVLINGKKLSGILIETPSDGNGLIVGIGLNVAVAPIATSICLRQVGLQASPKEVVSA
LLAEMHTIYQQLIALAAKDISFKDSECFGEIMRLCSTINKTVRVTGTKTFVGVAQGLDSLGRLVIRHSGDTSIVSAGDIE
HLRAIDSL

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=248, Percent_Identity=27.8225806451613, Blast_Score=80, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 26774; Mature: 26774

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPCVFLDTVGSTNLYLLELLKAKDLDDFFAVFTTNQTAGRGRMARSWECKKGIALSVFL
CCCEEEEECCCCCHHHHHHHHHHCCCCHHHEEEECCCCCCCCCCCCCCCCCCCCEEEEEE
KHNLPGAYINWLPLVIGLAVVKAVNKMLEDATLPHRADIKWPNDVLINGKKLSGILIETP
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCEECEEEEECC
SDGNGLIVGIGLNVAVAPIATSICLRQVGLQASPKEVVSALLAEMHTIYQQLIALAAKDI
CCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
SFKDSECFGEIMRLCSTINKTVRVTGTKTFVGVAQGLDSLGRLVIRHSGDTSIVSAGDIE
CCCCHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHEEEEECCCCCEEECCCHH
HLRAIDSL
HHHHHHCC
>Mature Secondary Structure
MFPCVFLDTVGSTNLYLLELLKAKDLDDFFAVFTTNQTAGRGRMARSWECKKGIALSVFL
CCCEEEEECCCCCHHHHHHHHHHCCCCHHHEEEECCCCCCCCCCCCCCCCCCCCEEEEEE
KHNLPGAYINWLPLVIGLAVVKAVNKMLEDATLPHRADIKWPNDVLINGKKLSGILIETP
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCEECEEEEECC
SDGNGLIVGIGLNVAVAPIATSICLRQVGLQASPKEVVSALLAEMHTIYQQLIALAAKDI
CCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
SFKDSECFGEIMRLCSTINKTVRVTGTKTFVGVAQGLDSLGRLVIRHSGDTSIVSAGDIE
CCCCHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHEEEEECCCCCEEECCCHH
HLRAIDSL
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]