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The map label for this gene is hisI [H]

Identifier: 27366191

GI number: 27366191

Start: 2979908

End: 2980543

Strand: Reverse

Name: hisI [H]

Synonym: VV1_2913

Alternate gene names: 27366191

Gene position: 2980543-2979908 (Counterclockwise)

Preceding gene: 27366192

Following gene: 161501802

Centisome position: 90.82

GC content: 49.53

Gene sequence:

>636_bases
ATGAGTTTTAAAACCGCCGAAGTCAGCTCGCTCGCTGAGCGAATCAACTGGGAGAAGGTGGACGGTCTGGTACCTGCCAT
CGTACAAGATTTCCAATCGAGCCAAGTGCTGATGATGGGTTACATGAACCAAGAAGCCTTGGTGAAAACGGGTGAAACTG
GCCAAGTAACGTTTTTCTCTCGTACCAAAGAACGTCTTTGGACCAAAGGGGAAACGTCAGGTAACGTACTGCAATTGGTG
AACATGTCGTTGGATTGTGATAACGACACACTGTTAGTGAAAGTCAACCCAATTGGGCCGACGTGTCATACGGGCACGAC
CACTTGCTGGGATGGTGATCCACAGGAAGAATCACAAATGGTGTGGCTTCATCAACTTGAGCAGCTACTGGCTGCCCGCA
AGAGTGCCGGTCCAGATTCCTCTTATACTGCCAGTCTGTATGCTCGTGGTACCAAGCGTATTTCGCAGAAAGTGGGCGAA
GAAGGCGTTGAAGTCGCGCTTGCGGCGACGTCGGGCGATAAGGCGGAGTTAGTGTGTGAATCGGCAGACTTGATTTACCA
CTTGCTGGTATTGCTGCAAGACCAAGGCCTATCGATGAATGACGTTATCAATAAGCTTAAAGAGCGTCATAAGTAA

Upstream 100 bases:

>100_bases
ATGTGGACGGTGCGCTGGCAGCATCGGTGTTTCACAAACAAGTCATCAATATTGGTGAATTAAAGCAGTATTTAAAGCAA
CAAGGCATTGAGGTACGACT

Downstream 100 bases:

>100_bases
GATAGAGACAAGGTCAAGGTTGATAGGGATTAGGGCCCTAGTAGAGAGTGCGCAGGGACTCTGCGCACTTCGTTTTTTAG
AAAGAGTTATGGAATTCAGG

Product: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein

Products: NA

Alternate protein names: Phosphoribosyl-AMP cyclohydrolase; PRA-CH; Phosphoribosyl-ATP pyrophosphatase; PRA-PH [H]

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MSFKTAEVSSLAERINWEKVDGLVPAIVQDFQSSQVLMMGYMNQEALVKTGETGQVTFFSRTKERLWTKGETSGNVLQLV
NMSLDCDNDTLLVKVNPIGPTCHTGTTTCWDGDPQEESQMVWLHQLEQLLAARKSAGPDSSYTASLYARGTKRISQKVGE
EGVEVALAATSGDKAELVCESADLIYHLLVLLQDQGLSMNDVINKLKERHK

Sequences:

>Translated_211_residues
MSFKTAEVSSLAERINWEKVDGLVPAIVQDFQSSQVLMMGYMNQEALVKTGETGQVTFFSRTKERLWTKGETSGNVLQLV
NMSLDCDNDTLLVKVNPIGPTCHTGTTTCWDGDPQEESQMVWLHQLEQLLAARKSAGPDSSYTASLYARGTKRISQKVGE
EGVEVALAATSGDKAELVCESADLIYHLLVLLQDQGLSMNDVINKLKERHK
>Mature_210_residues
SFKTAEVSSLAERINWEKVDGLVPAIVQDFQSSQVLMMGYMNQEALVKTGETGQVTFFSRTKERLWTKGETSGNVLQLVN
MSLDCDNDTLLVKVNPIGPTCHTGTTTCWDGDPQEESQMVWLHQLEQLLAARKSAGPDSSYTASLYARGTKRISQKVGEE
GVEVALAATSGDKAELVCESADLIYHLLVLLQDQGLSMNDVINKLKERHK

Specific function: Histidine biosynthesis; second step. Histidine biosynthesis; third step. [C]

COG id: COG0139

COG function: function code E; Phosphoribosyl-AMP cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PRA-PH family [H]

Homologues:

Organism=Escherichia coli, GI2367128, Length=197, Percent_Identity=66.497461928934, Blast_Score=268, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6319819, Length=198, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023019
- InterPro:   IPR008179
- InterPro:   IPR021130
- InterPro:   IPR002496
- ProDom:   PD002610 [H]

Pfam domain/function: PF01502 PRA-CH; PF01503 PRA-PH [H]

EC number: =3.5.4.19; =3.6.1.31 [H]

Molecular weight: Translated: 23283; Mature: 23151

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFKTAEVSSLAERINWEKVDGLVPAIVQDFQSSQVLMMGYMNQEALVKTGETGQVTFFS
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHEECCCCCEEEEEE
RTKERLWTKGETSGNVLQLVNMSLDCDNDTLLVKVNPIGPTCHTGTTTCWDGDPQEESQM
HHHHHHHCCCCCCCCEEEEEECEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHH
VWLHQLEQLLAARKSAGPDSSYTASLYARGTKRISQKVGEEGVEVALAATSGDKAELVCE
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEC
SADLIYHLLVLLQDQGLSMNDVINKLKERHK
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SFKTAEVSSLAERINWEKVDGLVPAIVQDFQSSQVLMMGYMNQEALVKTGETGQVTFFS
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHEECCCCCEEEEEE
RTKERLWTKGETSGNVLQLVNMSLDCDNDTLLVKVNPIGPTCHTGTTTCWDGDPQEESQM
HHHHHHHCCCCCCCCEEEEEECEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHH
VWLHQLEQLLAARKSAGPDSSYTASLYARGTKRISQKVGEEGVEVALAATSGDKAELVCE
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEC
SADLIYHLLVLLQDQGLSMNDVINKLKERHK
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]