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The map label for this gene is hisF1 [H]

Identifier: 27366192

GI number: 27366192

Start: 2980540

End: 2981313

Strand: Reverse

Name: hisF1 [H]

Synonym: VV1_2914

Alternate gene names: 27366192

Gene position: 2981313-2980540 (Counterclockwise)

Preceding gene: 27366193

Following gene: 27366191

Centisome position: 90.84

GC content: 47.16

Gene sequence:

>774_bases
ATGCTGGCAAAGCGAATAATTCCATGTCTTGATGTTCGTGATGGACAAGTGGTGAAGGGCGTTCAGTTTCGTAACCATGA
AATCATTGGTGATATCGTTCCTCTTGCTAAACGCTATGCGGAAGAAGGCGCTGACGAGCTTGTATTCTATGACATTACCG
CTTCAAGTGATGGTCGTGTCGTCGATAAAAGTTGGGTTTCTCGCGTTGCAGAAGTGATTGATATTCCGTTCTGTGTCGCT
GGTGGCATTAAGTCAGCAGAAGACGCAGCGCGCATTCTTGAGTTTGGCGCAGACAAAGTCTCTATCAACTCGCCAGCGCT
GGCTAACCCGCAGCTGATTACTGACCTTGCCGATAAGTTTGGTGTGCAATGTATCGTCGTTGGTATCGATTCATACTACG
ACAAAGAGACCGGTAAGTATCAGGTTTATCAGTTTACTGGTGACGAAGAACGCACCAAAGCGACCCAATGGGAAACACGT
GATTGGGTGCAAGAAGTACAAAAACGCGGCGCTGGTGAAATTGTATTGAACATGATGAACCAAGACGGCGTACGTAACGG
TTACGATATCGAACAACTGAATATGGTACGCGAAGTGTGTAAGGTGCCATTGATTGCCTCTGGTGGTGCCGGTGCCATGG
AGCACTTCGCGGAAGCGTACCAAAAAGCCAATGTGGACGGTGCGCTGGCAGCATCGGTGTTTCACAAACAAGTCATCAAT
ATTGGTGAATTAAAGCAGTATTTAAAGCAACAAGGCATTGAGGTACGACTATGA

Upstream 100 bases:

>100_bases
CATTAACGGATATCGAAGCACTAAAAGGCAGCGGTGTGGCTGGCGTGATTGTTGGTCGTGCACTGTTAGATGGCAAGTTC
ACAGCAGAGGAGGCATTTGC

Downstream 100 bases:

>100_bases
GTTTTAAAACCGCCGAAGTCAGCTCGCTCGCTGAGCGAATCAACTGGGAGAAGGTGGACGGTCTGGTACCTGCCATCGTA
CAAGATTTCCAATCGAGCCA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF1; ImGP synthase subunit hisF1; IGPS subunit hisF1 [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGADELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVA
GGIKSAEDAARILEFGADKVSINSPALANPQLITDLADKFGVQCIVVGIDSYYDKETGKYQVYQFTGDEERTKATQWETR
DWVQEVQKRGAGEIVLNMMNQDGVRNGYDIEQLNMVREVCKVPLIASGGAGAMEHFAEAYQKANVDGALAASVFHKQVIN
IGELKQYLKQQGIEVRL

Sequences:

>Translated_257_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGADELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVA
GGIKSAEDAARILEFGADKVSINSPALANPQLITDLADKFGVQCIVVGIDSYYDKETGKYQVYQFTGDEERTKATQWETR
DWVQEVQKRGAGEIVLNMMNQDGVRNGYDIEQLNMVREVCKVPLIASGGAGAMEHFAEAYQKANVDGALAASVFHKQVIN
IGELKQYLKQQGIEVRL
>Mature_257_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGADELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVA
GGIKSAEDAARILEFGADKVSINSPALANPQLITDLADKFGVQCIVVGIDSYYDKETGKYQVYQFTGDEERTKATQWETR
DWVQEVQKRGAGEIVLNMMNQDGVRNGYDIEQLNMVREVCKVPLIASGGAGAMEHFAEAYQKANVDGALAASVFHKQVIN
IGELKQYLKQQGIEVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=86.3813229571984, Blast_Score=465, Evalue=1e-132,
Organism=Escherichia coli, GI87082028, Length=219, Percent_Identity=26.9406392694064, Blast_Score=72, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319725, Length=317, Percent_Identity=31.5457413249211, Blast_Score=139, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 28431; Mature: 28431

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGADELVFYDITASSDGRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCE
VDKSWVSRVAEVIDIPFCVAGGIKSAEDAARILEFGADKVSINSPALANPQLITDLADKF
ECHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHH
GVQCIVVGIDSYYDKETGKYQVYQFTGDEERTKATQWETRDWVQEVQKRGAGEIVLNMMN
CCEEEEEECCHHCCCCCCCEEEEEECCCHHHHHHHCCHHHHHHHHHHHCCCCCEEHHHHH
QDGVRNGYDIEQLNMVREVCKVPLIASGGAGAMEHFAEAYQKANVDGALAASVFHKQVIN
CCCCCCCCCHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
IGELKQYLKQQGIEVRL
HHHHHHHHHHCCCEECC
>Mature Secondary Structure
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGADELVFYDITASSDGRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCE
VDKSWVSRVAEVIDIPFCVAGGIKSAEDAARILEFGADKVSINSPALANPQLITDLADKF
ECHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHH
GVQCIVVGIDSYYDKETGKYQVYQFTGDEERTKATQWETRDWVQEVQKRGAGEIVLNMMN
CCEEEEEECCHHCCCCCCCEEEEEECCCHHHHHHHCCHHHHHHHHHHHCCCCCEEHHHHH
QDGVRNGYDIEQLNMVREVCKVPLIASGGAGAMEHFAEAYQKANVDGALAASVFHKQVIN
CCCCCCCCCHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
IGELKQYLKQQGIEVRL
HHHHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA