Click here to switch to the map view.

The map label for this gene is mexA [H]

Identifier: 27366155

GI number: 27366155

Start: 2944169

End: 2945200

Strand: Reverse

Name: mexA [H]

Synonym: VV1_2876

Alternate gene names: 27366155

Gene position: 2945200-2944169 (Counterclockwise)

Preceding gene: 27366156

Following gene: 326424092

Centisome position: 89.74

GC content: 48.26

Gene sequence:

>1032_bases
ATGAGAAAAGCATTGGTTTTTCTTGCTGTCGCCACAGCATTGGGAGGATGTGGTCGAGAGCTTCCTCCTATTCCCGAGCC
TGATTCGCGGCCTGCCAAGTTATTCACGGTGTCGGTGGGTGACGCCAAATTTGAACGAACTTTTCCTGCTGTTTCTGAAG
CGGGTGATAAGGCAGTTTTAACATTTCGTGTCCCTGGACAATTGCAGACCATTGATGTTACTGCAGGTCAAAGAGTGACA
AAAGGGCAGGTTTTAGCCACCTTGAACCCAGATGAGTACGCGTTGCTGGCAAAACAAGCGAGAGCGAATTTTAAACTGGC
GGATGTGCAATATGAGCGCTACAAAAAGCTTCGCGCCGATAAGGTGGTCTCTGAGCAAGACTTCGATCAAGCACAAGCCA
ACCACAACTCTGCGCGCGCTACGTTAGAGCAAGCTGAGGCCAACCTTCGTTACACTAAGCTGATTGCACCTTATGATGGC
ACCATTTCTCTTATTCCAGCTGAAAATCACGAATACGTTGCGGCCAAACAAGGCGTAATGAATATCCAAACCAATCAATT
GATGAAGGTGATTTTCCAATTGCCAGATCATCTATTGGGCCGTTTCTCTCAGGGTGTTGAGCCGAACGCGGTGATGCGTT
TTGACGCATTCCCAGGGAGCGAATTCCCCCTGCGCTTCCAAGAAATCGATACCGAAGCGGATACCAAAACCGGCAGTTAT
AAAGTCACTATGATCATGGAGCGTCCTGCCGATCTTGGGGTGCTCCCCGGTATGGCTGGTAGTGTCCACGTGAGTGCCAA
ATCTCAATCTGCCACACAAATCCCTACCTCTGCCATCTTTAAGCAAGATGAGAAAAAGTATGTTTGGCGAGTGAATGATC
AGAGCATTGTTGAGCTCGCGGAAGTGGAGCTTAACGACAAGCGCGAAGTGGTTAATGGTCTCAATGATGGTGACCAAATC
ATTATTTCTGGTGTCAGTGGTATCGAGTCAGGGATAAAAGTTCGTGAATGGGTTAAAGAGCGAGGGCTATAA

Upstream 100 bases:

>100_bases
CTCATTTTATTAAATTTCAGATATTTACAAAGCTGTTAACTGGAACAAAATCATAGTTTCATCTAAGGTATAGAGAACAT
TTTCTAACAGGATGATAACC

Downstream 100 bases:

>100_bases
CGTGAATAAATGGATTGGTTTAACCGCGTTGTCCGCCGCTTTACTGTTAGCCGGATGTGGGCAACAAAATCAGCCAGCCG
CTATGGCTGACCCAAATGTC

Product: membrane-fusion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MRKALVFLAVATALGGCGRELPPIPEPDSRPAKLFTVSVGDAKFERTFPAVSEAGDKAVLTFRVPGQLQTIDVTAGQRVT
KGQVLATLNPDEYALLAKQARANFKLADVQYERYKKLRADKVVSEQDFDQAQANHNSARATLEQAEANLRYTKLIAPYDG
TISLIPAENHEYVAAKQGVMNIQTNQLMKVIFQLPDHLLGRFSQGVEPNAVMRFDAFPGSEFPLRFQEIDTEADTKTGSY
KVTMIMERPADLGVLPGMAGSVHVSAKSQSATQIPTSAIFKQDEKKYVWRVNDQSIVELAEVELNDKREVVNGLNDGDQI
IISGVSGIESGIKVREWVKERGL

Sequences:

>Translated_343_residues
MRKALVFLAVATALGGCGRELPPIPEPDSRPAKLFTVSVGDAKFERTFPAVSEAGDKAVLTFRVPGQLQTIDVTAGQRVT
KGQVLATLNPDEYALLAKQARANFKLADVQYERYKKLRADKVVSEQDFDQAQANHNSARATLEQAEANLRYTKLIAPYDG
TISLIPAENHEYVAAKQGVMNIQTNQLMKVIFQLPDHLLGRFSQGVEPNAVMRFDAFPGSEFPLRFQEIDTEADTKTGSY
KVTMIMERPADLGVLPGMAGSVHVSAKSQSATQIPTSAIFKQDEKKYVWRVNDQSIVELAEVELNDKREVVNGLNDGDQI
IISGVSGIESGIKVREWVKERGL
>Mature_343_residues
MRKALVFLAVATALGGCGRELPPIPEPDSRPAKLFTVSVGDAKFERTFPAVSEAGDKAVLTFRVPGQLQTIDVTAGQRVT
KGQVLATLNPDEYALLAKQARANFKLADVQYERYKKLRADKVVSEQDFDQAQANHNSARATLEQAEANLRYTKLIAPYDG
TISLIPAENHEYVAAKQGVMNIQTNQLMKVIFQLPDHLLGRFSQGVEPNAVMRFDAFPGSEFPLRFQEIDTEADTKTGSY
KVTMIMERPADLGVLPGMAGSVHVSAKSQSATQIPTSAIFKQDEKKYVWRVNDQSIVELAEVELNDKREVVNGLNDGDQI
IISGVSGIESGIKVREWVKERGL

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87082036, Length=306, Percent_Identity=23.8562091503268, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1789665, Length=372, Percent_Identity=26.6129032258064, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87081796, Length=150, Percent_Identity=34, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 37763; Mature: 37763

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKALVFLAVATALGGCGRELPPIPEPDSRPAKLFTVSVGDAKFERTFPAVSEAGDKAVL
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHCCCHHHCCCCEEEE
TFRVPGQLQTIDVTAGQRVTKGQVLATLNPDEYALLAKQARANFKLADVQYERYKKLRAD
EEECCCCEEEEEECCCCEECCCCEEEEECCCHHHHHHHHHHCCEEEECCCHHHHHHHHHH
KVVSEQDFDQAQANHNSARATLEQAEANLRYTKLIAPYDGTISLIPAENHEYVAAKQGVM
HHCCCCCHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCCEEEECCCCE
NIQTNQLMKVIFQLPDHLLGRFSQGVEPNAVMRFDAFPGSEFPLRFQEIDTEADTKTGSY
ECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEHEECCCCCCCCCCCE
KVTMIMERPADLGVLPGMAGSVHVSAKSQSATQIPTSAIFKQDEKKYVWRVNDQSIVELA
EEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHCCCCCEEEEEECCHHHHHHH
EVELNDKREVVNGLNDGDQIIISGVSGIESGIKVREWVKERGL
HCCCCCHHHHHCCCCCCCEEEEEECHHHHHCHHHHHHHHHCCC
>Mature Secondary Structure
MRKALVFLAVATALGGCGRELPPIPEPDSRPAKLFTVSVGDAKFERTFPAVSEAGDKAVL
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHCCCHHHCCCCEEEE
TFRVPGQLQTIDVTAGQRVTKGQVLATLNPDEYALLAKQARANFKLADVQYERYKKLRAD
EEECCCCEEEEEECCCCEECCCCEEEEECCCHHHHHHHHHHCCEEEECCCHHHHHHHHHH
KVVSEQDFDQAQANHNSARATLEQAEANLRYTKLIAPYDGTISLIPAENHEYVAAKQGVM
HHCCCCCHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCCEEEECCCCE
NIQTNQLMKVIFQLPDHLLGRFSQGVEPNAVMRFDAFPGSEFPLRFQEIDTEADTKTGSY
ECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEHEECCCCCCCCCCCE
KVTMIMERPADLGVLPGMAGSVHVSAKSQSATQIPTSAIFKQDEKKYVWRVNDQSIVELA
EEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHCCCCCEEEEEECCHHHHHHH
EVELNDKREVVNGLNDGDQIIISGVSGIESGIKVREWVKERGL
HCCCCCHHHHHCCCCCCCEEEEEECHHHHHCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]