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The map label for this gene is dpiA [H]

Identifier: 27365977

GI number: 27365977

Start: 2733091

End: 2733777

Strand: Reverse

Name: dpiA [H]

Synonym: VV1_2685

Alternate gene names: 27365977

Gene position: 2733777-2733091 (Counterclockwise)

Preceding gene: 27365978

Following gene: 27365976

Centisome position: 83.3

GC content: 48.62

Gene sequence:

>687_bases
ATGAATGCCGATACCCGAGTGATGATAATTGAAGATGATATGGCGATTGCTCAGCTTCATCATAAGTATTTGCAGCAAAT
GGATGGTTTCGAGGTGATTGGCATTGCGACCACACAAGCCGAAGCCGAAATGCAGTTGTCCGTGTTCGAGCCTGATTTGG
TTTTGCTCGATGTTTACCTTCCTGATGGCAGTGGGTTGGAGATCCTCAATCAAATTCGCGGCAGCAATCGTCATTGTGAC
GTTATTTTGATTACCGCAGCAAGAGATGTGGAAACGCTACAAACCGCAATGCGTGGCGGTGTGGTGGACTATTTGTTAAA
ACCCGTGATGTTTCCGCGGCTGGAAAGTGCTTTGAACAAATACCGTGCGCAACGAGTGCAGTTGGGAACCGTGTCCGACC
TTAATCAAGGCTTGGTGGATAAAATGCTGCAAGCAAGCAGTACGCAGGAAAGCGTATCCAATCGGCTACCCAAAGGTATC
GATGGGGTTACCCTTGATAAAATTCGAACCTTATTTCAAGGCGAGGTTTCCTATACTGCAGACGAAGCGGGGGAGCAAAT
CGGGGCCAGTCGCACCACGGCAAGGCGCTATCTTGAATATTTGATCAGCTCGGGTGAGCTTGCTGCCGATTTGAATTACG
GTACGGTTGGTCGCCCTGAACGAACGTATCGCAAAGTTCATCGTTAA

Upstream 100 bases:

>100_bases
TCAATCAATTAGCCGCACGTTATTGCGGCTCGATAGAGATGGCTGAGAATGACAAATTTGGCACAAGAATGACGGTCTAT
TTACCCAAGGAAGAACAAAG

Downstream 100 bases:

>100_bases
TTTGCTGGTTATGCGGCAAATTTAATATGTGAGCGGTGTTTTTGTTACTTATCGTGCAATGGATTACATTTGGGCGGATA
TTTGCTGATTATTTGCCCAC

Product: transcriptional regulator CitB

Products: NA

Alternate protein names: Destabilizer of plasmid inheritance [H]

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MNADTRVMIIEDDMAIAQLHHKYLQQMDGFEVIGIATTQAEAEMQLSVFEPDLVLLDVYLPDGSGLEILNQIRGSNRHCD
VILITAARDVETLQTAMRGGVVDYLLKPVMFPRLESALNKYRAQRVQLGTVSDLNQGLVDKMLQASSTQESVSNRLPKGI
DGVTLDKIRTLFQGEVSYTADEAGEQIGASRTTARRYLEYLISSGELAADLNYGTVGRPERTYRKVHR

Sequences:

>Translated_228_residues
MNADTRVMIIEDDMAIAQLHHKYLQQMDGFEVIGIATTQAEAEMQLSVFEPDLVLLDVYLPDGSGLEILNQIRGSNRHCD
VILITAARDVETLQTAMRGGVVDYLLKPVMFPRLESALNKYRAQRVQLGTVSDLNQGLVDKMLQASSTQESVSNRLPKGI
DGVTLDKIRTLFQGEVSYTADEAGEQIGASRTTARRYLEYLISSGELAADLNYGTVGRPERTYRKVHR
>Mature_228_residues
MNADTRVMIIEDDMAIAQLHHKYLQQMDGFEVIGIATTQAEAEMQLSVFEPDLVLLDVYLPDGSGLEILNQIRGSNRHCD
VILITAARDVETLQTAMRGGVVDYLLKPVMFPRLESALNKYRAQRVQLGTVSDLNQGLVDKMLQASSTQESVSNRLPKGI
DGVTLDKIRTLFQGEVSYTADEAGEQIGASRTTARRYLEYLISSGELAADLNYGTVGRPERTYRKVHR

Specific function: Member of the two-component regulatory system DpiA/DpiB, which is essential for expression of citrate-specific fermentation genes and genes involved in plasmid inheritance. Could be involved in response to both the presence of citrate and external redox c

COG id: COG4565

COG function: function code KT; Response regulator of citrate/malate metabolism

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786838, Length=224, Percent_Identity=39.2857142857143, Blast_Score=175, Evalue=2e-45,
Organism=Escherichia coli, GI1790566, Length=225, Percent_Identity=35.1111111111111, Blast_Score=143, Evalue=8e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 25378; Mature: 25378

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNADTRVMIIEDDMAIAQLHHKYLQQMDGFEVIGIATTQAEAEMQLSVFEPDLVLLDVYL
CCCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEECCCEEEEEEEC
PDGSGLEILNQIRGSNRHCDVILITAARDVETLQTAMRGGVVDYLLKPVMFPRLESALNK
CCCCCHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
YRAQRVQLGTVSDLNQGLVDKMLQASSTQESVSNRLPKGIDGVTLDKIRTLFQGEVSYTA
HHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCH
DEAGEQIGASRTTARRYLEYLISSGELAADLNYGTVGRPERTYRKVHR
HHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MNADTRVMIIEDDMAIAQLHHKYLQQMDGFEVIGIATTQAEAEMQLSVFEPDLVLLDVYL
CCCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEECCCEEEEEEEC
PDGSGLEILNQIRGSNRHCDVILITAARDVETLQTAMRGGVVDYLLKPVMFPRLESALNK
CCCCCHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
YRAQRVQLGTVSDLNQGLVDKMLQASSTQESVSNRLPKGIDGVTLDKIRTLFQGEVSYTA
HHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCH
DEAGEQIGASRTTARRYLEYLISSGELAADLNYGTVGRPERTYRKVHR
HHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]