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The map label for this gene is citS [H]

Identifier: 27365978

GI number: 27365978

Start: 2733774

End: 2735393

Strand: Reverse

Name: citS [H]

Synonym: VV1_2686

Alternate gene names: 27365978

Gene position: 2735393-2733774 (Counterclockwise)

Preceding gene: 27365985

Following gene: 27365977

Centisome position: 83.35

GC content: 49.01

Gene sequence:

>1620_bases
ATGACGTGGCAAAGCATCAGTTTTCGTAAAAGAATGCTGGTCATTATGACGCTCTCCGGATTAATTGAGTTGCTTATCCT
CGTAGCAGCGGGCTTTGCCTACCTCAAATACTCGCAAGAGCAAGAAATGGGGCAGAAGGCACTCGGTGTCGCGCGATTTC
TTGCTACCTCTACGACGGTTATCGAGTTGATCGAAAATCGTGACGATGTCACCTATCAGCAAAAATTTCGAGAACTAACC
CAAGCATTGGGCGCAGCCTTCATTGTGGTGGGTGATCGTGAAGGTATTCGGTTGATCCACCCCGTTGATGAACGCCTTGG
TTTGCCGATGCGTGGTGGTGATAACGCCCGAGCATTGGAAGAAGGCGAAGCGTATATCTCGACAGCAAGAGGTTCACTGG
GCTATTCGGTGCGTGGCAAAGCAGCGGTTTTTAACTCGGCGGGTGAAGTGATCGGTGTGGTTTCTGTGGGTTATCTGCTG
GATCGATTACAAGACAGAATAGAGCCATTTGCGTGGTTTTTAATTGCTATGACGTTACTGGTGGTTGCTGCGAATGCCTT
GGTTTCCAATTACGCTTCTCGTAAATTTCAGCGAGCGATTTTGGGGTTTGAACCGGAAGAAATTGGTCGGTTATATGGTG
AGCTTGATGTGACCATGAGCACCATTAAAGAAGGGGTGTTAAGTATTGATTCGAGTGGCGTGCTGCGTTCCATCAACCGC
AGTGCAGCGCAGATTCTCGGGTTAGATCCGGCCCAAGTGCTGAACAAACCGCTCTCTGATGCGTTGCCCGCCAGTGATCT
GTACCGTGTATTAGAAACGGGACAAGAAGATCACGATATTGAACTTTACCTCAACCAAAAGAGGTTGATTGCCAACCGAA
GCCCGGTTTACGTAGAGGGAAAGATTGTCGGCGCGGTGTCCAGTTTTCGTTTGCGTGATGAAATTTCTGAGCTCACTGAA
CAGCTTTCTCAAACCAAAGAGTATGCCGATTTATTACGCTCACAAACGCATGAACATCGCAACAAACTTAATACCATCAG
TGGCCTAGTTCAGATGGGCGAGCTTGATGCGGTTCAACAGCTTATCGGGCAGGAGACGGCGCACTATCAGGGGTTGATCG
AGTTTCTACGCGATACCATCAAAGATCCGTTGGTCGCGGGCATGCTGCTAGGAAAAACGGAACGCGCGCGTGAGCTTGGC
TTAGAGTTAGTGGTTGAAGAAGGGGCTCGTTTAGAGCCGCTAAGTGCTTGGCTGAACCCAGAAGACATCACGACAATACT
CGGTAACCTGATTGATAACGCTTTTGACGCGACCATGAGCGCGATCGCGCAAGAAGGCAATTTTGCCCGTTCACGCAGAA
CCATTGAAGTTTCCATCAGTGACTACGGAACGGAAGTGATTCTTGAAGTGCAGGATCAAGGTTGTGGTTTGCCCAAACAA
TTTTCCACCGAGCAGCTGCTTGAGAAAGGGATCTCGAGCAAAGCGACCAGCACTCGGGGTGTTGGGCTCTACTTAGTCAA
TCAATTAGCCGCACGTTATTGCGGCTCGATAGAGATGGCTGAGAATGACAAATTTGGCACAAGAATGACGGTCTATTTAC
CCAAGGAAGAACAAAGATGA

Upstream 100 bases:

>100_bases
TTGCGATTCACACTTCCGGAGGGTGGTAAGAGCTTGCATGTTAAAACGGTGTTAAGTTAACTGAGTTTTCGATGTTTTTA
AGTGGTGCAAAGAGGTAAGC

Downstream 100 bases:

>100_bases
ATGCCGATACCCGAGTGATGATAATTGAAGATGATATGGCGATTGCTCAGCTTCATCATAAGTATTTGCAGCAAATGGAT
GGTTTCGAGGTGATTGGCAT

Product: sensor kinase citA, dpiB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MTWQSISFRKRMLVIMTLSGLIELLILVAAGFAYLKYSQEQEMGQKALGVARFLATSTTVIELIENRDDVTYQQKFRELT
QALGAAFIVVGDREGIRLIHPVDERLGLPMRGGDNARALEEGEAYISTARGSLGYSVRGKAAVFNSAGEVIGVVSVGYLL
DRLQDRIEPFAWFLIAMTLLVVAANALVSNYASRKFQRAILGFEPEEIGRLYGELDVTMSTIKEGVLSIDSSGVLRSINR
SAAQILGLDPAQVLNKPLSDALPASDLYRVLETGQEDHDIELYLNQKRLIANRSPVYVEGKIVGAVSSFRLRDEISELTE
QLSQTKEYADLLRSQTHEHRNKLNTISGLVQMGELDAVQQLIGQETAHYQGLIEFLRDTIKDPLVAGMLLGKTERARELG
LELVVEEGARLEPLSAWLNPEDITTILGNLIDNAFDATMSAIAQEGNFARSRRTIEVSISDYGTEVILEVQDQGCGLPKQ
FSTEQLLEKGISSKATSTRGVGLYLVNQLAARYCGSIEMAENDKFGTRMTVYLPKEEQR

Sequences:

>Translated_539_residues
MTWQSISFRKRMLVIMTLSGLIELLILVAAGFAYLKYSQEQEMGQKALGVARFLATSTTVIELIENRDDVTYQQKFRELT
QALGAAFIVVGDREGIRLIHPVDERLGLPMRGGDNARALEEGEAYISTARGSLGYSVRGKAAVFNSAGEVIGVVSVGYLL
DRLQDRIEPFAWFLIAMTLLVVAANALVSNYASRKFQRAILGFEPEEIGRLYGELDVTMSTIKEGVLSIDSSGVLRSINR
SAAQILGLDPAQVLNKPLSDALPASDLYRVLETGQEDHDIELYLNQKRLIANRSPVYVEGKIVGAVSSFRLRDEISELTE
QLSQTKEYADLLRSQTHEHRNKLNTISGLVQMGELDAVQQLIGQETAHYQGLIEFLRDTIKDPLVAGMLLGKTERARELG
LELVVEEGARLEPLSAWLNPEDITTILGNLIDNAFDATMSAIAQEGNFARSRRTIEVSISDYGTEVILEVQDQGCGLPKQ
FSTEQLLEKGISSKATSTRGVGLYLVNQLAARYCGSIEMAENDKFGTRMTVYLPKEEQR
>Mature_538_residues
TWQSISFRKRMLVIMTLSGLIELLILVAAGFAYLKYSQEQEMGQKALGVARFLATSTTVIELIENRDDVTYQQKFRELTQ
ALGAAFIVVGDREGIRLIHPVDERLGLPMRGGDNARALEEGEAYISTARGSLGYSVRGKAAVFNSAGEVIGVVSVGYLLD
RLQDRIEPFAWFLIAMTLLVVAANALVSNYASRKFQRAILGFEPEEIGRLYGELDVTMSTIKEGVLSIDSSGVLRSINRS
AAQILGLDPAQVLNKPLSDALPASDLYRVLETGQEDHDIELYLNQKRLIANRSPVYVEGKIVGAVSSFRLRDEISELTEQ
LSQTKEYADLLRSQTHEHRNKLNTISGLVQMGELDAVQQLIGQETAHYQGLIEFLRDTIKDPLVAGMLLGKTERARELGL
ELVVEEGARLEPLSAWLNPEDITTILGNLIDNAFDATMSAIAQEGNFARSRRTIEVSISDYGTEVILEVQDQGCGLPKQF
STEQLLEKGISSKATSTRGVGLYLVNQLAARYCGSIEMAENDKFGTRMTVYLPKEEQR

Specific function: Member of the two-component regulatory system CitT/CitS. Functions probably as a membrane-associated protein kinase that phosphorylates CitT in response to environmental citrate or Mg(2+)-citrate complex [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786837, Length=541, Percent_Identity=33.641404805915, Blast_Score=323, Evalue=2e-89,
Organism=Escherichia coli, GI1790567, Length=546, Percent_Identity=29.8534798534799, Blast_Score=205, Evalue=5e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 59606; Mature: 59475

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTWQSISFRKRMLVIMTLSGLIELLILVAAGFAYLKYSQEQEMGQKALGVARFLATSTTV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
IELIENRDDVTYQQKFRELTQALGAAFIVVGDREGIRLIHPVDERLGLPMRGGDNARALE
HHHHHCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCEEECCCHHHCCCCCCCCCCCCHHH
EGEAYISTARGSLGYSVRGKAAVFNSAGEVIGVVSVGYLLDRLQDRIEPFAWFLIAMTLL
HCHHHHHHHCCCCCCCCCCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVAANALVSNYASRKFQRAILGFEPEEIGRLYGELDVTMSTIKEGVLSIDSSGVLRSINR
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
SAAQILGLDPAQVLNKPLSDALPASDLYRVLETGQEDHDIELYLNQKRLIANRSPVYVEG
HHHHHHCCCHHHHHCCCHHHCCCHHHHHHHHHCCCCCCCEEEEECCHHHHCCCCCEEEEE
KIVGAVSSFRLRDEISELTEQLSQTKEYADLLRSQTHEHRNKLNTISGLVQMGELDAVQQ
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIGQETAHYQGLIEFLRDTIKDPLVAGMLLGKTERARELGLELVVEEGARLEPLSAWLNP
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCHHHEECCCCCCCHHHHCCCH
EDITTILGNLIDNAFDATMSAIAQEGNFARSRRTIEVSISDYGTEVILEVQDQGCGLPKQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEEEEECCCCCEEEEEECCCCCCCCCC
FSTEQLLEKGISSKATSTRGVGLYLVNQLAARYCGSIEMAENDKFGTRMTVYLPKEEQR
CCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCEEEEEECCCCCC
>Mature Secondary Structure 
TWQSISFRKRMLVIMTLSGLIELLILVAAGFAYLKYSQEQEMGQKALGVARFLATSTTV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
IELIENRDDVTYQQKFRELTQALGAAFIVVGDREGIRLIHPVDERLGLPMRGGDNARALE
HHHHHCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCEEECCCHHHCCCCCCCCCCCCHHH
EGEAYISTARGSLGYSVRGKAAVFNSAGEVIGVVSVGYLLDRLQDRIEPFAWFLIAMTLL
HCHHHHHHHCCCCCCCCCCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVAANALVSNYASRKFQRAILGFEPEEIGRLYGELDVTMSTIKEGVLSIDSSGVLRSINR
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
SAAQILGLDPAQVLNKPLSDALPASDLYRVLETGQEDHDIELYLNQKRLIANRSPVYVEG
HHHHHHCCCHHHHHCCCHHHCCCHHHHHHHHHCCCCCCCEEEEECCHHHHCCCCCEEEEE
KIVGAVSSFRLRDEISELTEQLSQTKEYADLLRSQTHEHRNKLNTISGLVQMGELDAVQQ
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIGQETAHYQGLIEFLRDTIKDPLVAGMLLGKTERARELGLELVVEEGARLEPLSAWLNP
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCHHHEECCCCCCCHHHHCCCH
EDITTILGNLIDNAFDATMSAIAQEGNFARSRRTIEVSISDYGTEVILEVQDQGCGLPKQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEEEEECCCCCEEEEEECCCCCCCCCC
FSTEQLLEKGISSKATSTRGVGLYLVNQLAARYCGSIEMAENDKFGTRMTVYLPKEEQR
CCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10484179; 11058132 [H]