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The map label for this gene is fmt

Identifier: 27364479

GI number: 27364479

Start: 1050223

End: 1051170

Strand: Reverse

Name: fmt

Synonym: VV1_1047

Alternate gene names: 27364479

Gene position: 1051170-1050223 (Counterclockwise)

Preceding gene: 27364480

Following gene: 27364478

Centisome position: 32.03

GC content: 51.69

Gene sequence:

>948_bases
TTGAGCAAGCCTTTACGTATTGTATTTGCAGGTACTCCGGACTTCGCCGCCCAACATTTGGCGGCGTTGTTGTCTTCGGA
GCATGAAGTCATTGCAGTTTACACCCAACCTGATCGCCCAGCCGGTCGCGGGAAAAAACTCACAGCAAGCCCTGTCAAAA
CCATTGCTTTGGAACACAACATTCCGGTTTACCAACCAGAAAACTTTAAGTCTGACGAAGCCAAGCAAGCCCTCGCGGAT
CTCAATGCTGACATCATGGTCGTGGTCGCTTATGGCTTGTTATTACCACAAGCGGTGTTAGATACACCGAAATTGGGTTG
TATCAATGTCCACGGCTCGATTCTGCCTCGCTGGCGTGGCGCAGCACCGATTCAGCGCTCTATTTGGGCGGGTGATGCTG
AAACGGGCGTGACCATCATGCAAATGGACATCGGCTTAGATACCGGCGACATGCTGAAAATTGCCACGCTGCCGATCGAC
GCAAGCGATACCAGCGCCACCATGTACGATAAATTGGCCAAGCTTGGCCCTGTGGTACTGGTTGAGTGTTTGGCAGATAT
CGCCGCTGGCACAGCCATCGCAATTAAGCAAGATGACGAGCGAGCAAACTACGCCAAGAAACTCAGCAAGGAAGAAGCAC
GCATCAATTGGCAAGACGATGCAGAGCACATCGAACGTTGTGTTCGTGCTTTCAATCCATGGCCAATGAGCCATTTCGAA
GTGGCAGAAAACAGCATTAAAGTGTGGCAAAGCCGCGTCGAAGCCAGCAGTCATGACGCACCCGCGGGGACCATCCTCAA
AGCGGATAAAAGCGGCATTTATGTCGCCACTGGCCATGGCTGCCTAGTATTGGAGCAGATCCAAATTCCAGGCAAAAAAG
CGATGCCAGTACAAGATGTTCTCAACGCTCGCGCCGCTTGGTTTGAAGTTGGCAGTGTACTGAGTTAA

Upstream 100 bases:

>100_bases
CCGCTAAAGCGCAAGCGTATCCAAGACAAGCTAGCCAAAATCAAACGCTTTAACGAGAAACAGCGCTAAATTGCGCTGAT
ACCATAAGAAGGAAGGTACC

Downstream 100 bases:

>100_bases
CCTGCCAAGCAATCGGGCATTTTTGCCCAGTTTAAGAACTTTACGAGGGCAGAGACGCCCTCTTTAACTTTAAGGTACTC
ATCATGAATGTTCGCGCTGC

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MSKPLRIVFAGTPDFAAQHLAALLSSEHEVIAVYTQPDRPAGRGKKLTASPVKTIALEHNIPVYQPENFKSDEAKQALAD
LNADIMVVVAYGLLLPQAVLDTPKLGCINVHGSILPRWRGAAPIQRSIWAGDAETGVTIMQMDIGLDTGDMLKIATLPID
ASDTSATMYDKLAKLGPVVLVECLADIAAGTAIAIKQDDERANYAKKLSKEEARINWQDDAEHIERCVRAFNPWPMSHFE
VAENSIKVWQSRVEASSHDAPAGTILKADKSGIYVATGHGCLVLEQIQIPGKKAMPVQDVLNARAAWFEVGSVLS

Sequences:

>Translated_315_residues
MSKPLRIVFAGTPDFAAQHLAALLSSEHEVIAVYTQPDRPAGRGKKLTASPVKTIALEHNIPVYQPENFKSDEAKQALAD
LNADIMVVVAYGLLLPQAVLDTPKLGCINVHGSILPRWRGAAPIQRSIWAGDAETGVTIMQMDIGLDTGDMLKIATLPID
ASDTSATMYDKLAKLGPVVLVECLADIAAGTAIAIKQDDERANYAKKLSKEEARINWQDDAEHIERCVRAFNPWPMSHFE
VAENSIKVWQSRVEASSHDAPAGTILKADKSGIYVATGHGCLVLEQIQIPGKKAMPVQDVLNARAAWFEVGSVLS
>Mature_314_residues
SKPLRIVFAGTPDFAAQHLAALLSSEHEVIAVYTQPDRPAGRGKKLTASPVKTIALEHNIPVYQPENFKSDEAKQALADL
NADIMVVVAYGLLLPQAVLDTPKLGCINVHGSILPRWRGAAPIQRSIWAGDAETGVTIMQMDIGLDTGDMLKIATLPIDA
SDTSATMYDKLAKLGPVVLVECLADIAAGTAIAIKQDDERANYAKKLSKEEARINWQDDAEHIERCVRAFNPWPMSHFEV
AENSIKVWQSRVEASSHDAPAGTILKADKSGIYVATGHGCLVLEQIQIPGKKAMPVQDVLNARAAWFEVGSVLS

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI164663775, Length=255, Percent_Identity=34.5098039215686, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI21614513, Length=261, Percent_Identity=29.8850574712644, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI238814322, Length=283, Percent_Identity=28.6219081272085, Blast_Score=105, Evalue=5e-23,
Organism=Escherichia coli, GI1789683, Length=314, Percent_Identity=62.4203821656051, Blast_Score=405, Evalue=1e-114,
Organism=Escherichia coli, GI1788589, Length=277, Percent_Identity=29.2418772563177, Blast_Score=125, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI133930964, Length=317, Percent_Identity=24.9211356466877, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI45550868, Length=305, Percent_Identity=28.5245901639344, Blast_Score=107, Evalue=8e-24,
Organism=Drosophila melanogaster, GI28571984, Length=225, Percent_Identity=30.2222222222222, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585660, Length=243, Percent_Identity=32.0987654320988, Blast_Score=94, Evalue=9e-20,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_VIBVU (Q8DDE4)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_760007.1
- ProteinModelPortal:   Q8DDE4
- SMR:   Q8DDE4
- GeneID:   1177980
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_1047
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- ProtClustDB:   PRK00005
- BioCyc:   VVUL216895:VV1_1047-MONOMER
- BRENDA:   2.1.2.9
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 34079; Mature: 33948

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPLRIVFAGTPDFAAQHLAALLSSEHEVIAVYTQPDRPAGRGKKLTASPVKTIALEHN
CCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEECCCCEEEEEECC
IPVYQPENFKSDEAKQALADLNADIMVVVAYGLLLPQAVLDTPKLGCINVHGSILPRWRG
CCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCC
AAPIQRSIWAGDAETGVTIMQMDIGLDTGDMLKIATLPIDASDTSATMYDKLAKLGPVVL
CCCHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHH
VECLADIAAGTAIAIKQDDERANYAKKLSKEEARINWQDDAEHIERCVRAFNPWPMSHFE
HHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHH
VAENSIKVWQSRVEASSHDAPAGTILKADKSGIYVATGHGCLVLEQIQIPGKKAMPVQDV
HHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEECCCEEEEEEECCCCCCCCCHHHH
LNARAAWFEVGSVLS
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKPLRIVFAGTPDFAAQHLAALLSSEHEVIAVYTQPDRPAGRGKKLTASPVKTIALEHN
CCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEECCCCEEEEEECC
IPVYQPENFKSDEAKQALADLNADIMVVVAYGLLLPQAVLDTPKLGCINVHGSILPRWRG
CCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCC
AAPIQRSIWAGDAETGVTIMQMDIGLDTGDMLKIATLPIDASDTSATMYDKLAKLGPVVL
CCCHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHH
VECLADIAAGTAIAIKQDDERANYAKKLSKEEARINWQDDAEHIERCVRAFNPWPMSHFE
HHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHH
VAENSIKVWQSRVEASSHDAPAGTILKADKSGIYVATGHGCLVLEQIQIPGKKAMPVQDV
HHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEECCCEEEEEEECCCCCCCCCHHHH
LNARAAWFEVGSVLS
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA