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Identifier: 27364480

GI number: 27364480

Start: 1051202

End: 1051714

Strand: Reverse

Name: def

Synonym: VV1_1048

Alternate gene names: 27364480

Gene position: 1051714-1051202 (Counterclockwise)

Preceding gene: 27364485

Following gene: 27364479

Centisome position: 32.05

GC content: 46.39

Gene sequence:

>513_bases
ATGTCTGTATTACAAGTATTAACATTCCCGGATGATCGTCTTCGTACAGTAGCCAAACCGGTTGAAAAGGTGACACCAGA
AATCCAAAAAATCGTCGATGACATGATTGAAACCATGTATGACGAAGAAGGTATTGGCCTTGCCGCAACGCAAGTCGACA
TCCATCAACGTATTGTGGTGATTGATATTTCTGAGAGCCGTAACGAGCCGATGGTGTTAATCAATCCTGAAATTTTAGAA
AAGCGTGGCGAAGATGGCATTGAAGAGGGCTGTCTATCGGTCCCAGGTGCCCGCGCACTCGTGCCACGCGCCGCTGAAGT
CACGGTTAAAGCGTTAGATCGTGATGGTCATGAATTTACCCTCGAAGCCGATGATTTGCTGGCTATCTGTATTCAACACG
AATTGGATCACCTGCAAGGTAAGCTGTTTGTCGACTACCTATCACCGCTAAAGCGCAAGCGTATCCAAGACAAGCTAGCC
AAAATCAAACGCTTTAACGAGAAACAGCGCTAA

Upstream 100 bases:

>100_bases
GAAATTGCTGCTAGGATGCTGTCATTTAGGCTATAAAATGTCTAGAATTGAGCCAACAAGGTTTGTGCTGTTTCGGCACA
GTTCAACATTTCGAGTGCAT

Downstream 100 bases:

>100_bases
ATTGCGCTGATACCATAAGAAGGAAGGTACCTTGAGCAAGCCTTTACGTATTGTATTTGCAGGTACTCCGGACTTCGCCG
CCCAACATTTGGCGGCGTTG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2

Number of amino acids: Translated: 170; Mature: 169

Protein sequence:

>170_residues
MSVLQVLTFPDDRLRTVAKPVEKVTPEIQKIVDDMIETMYDEEGIGLAATQVDIHQRIVVIDISESRNEPMVLINPEILE
KRGEDGIEEGCLSVPGARALVPRAAEVTVKALDRDGHEFTLEADDLLAICIQHELDHLQGKLFVDYLSPLKRKRIQDKLA
KIKRFNEKQR

Sequences:

>Translated_170_residues
MSVLQVLTFPDDRLRTVAKPVEKVTPEIQKIVDDMIETMYDEEGIGLAATQVDIHQRIVVIDISESRNEPMVLINPEILE
KRGEDGIEEGCLSVPGARALVPRAAEVTVKALDRDGHEFTLEADDLLAICIQHELDHLQGKLFVDYLSPLKRKRIQDKLA
KIKRFNEKQR
>Mature_169_residues
SVLQVLTFPDDRLRTVAKPVEKVTPEIQKIVDDMIETMYDEEGIGLAATQVDIHQRIVVIDISESRNEPMVLINPEILEK
RGEDGIEEGCLSVPGARALVPRAAEVTVKALDRDGHEFTLEADDLLAICIQHELDHLQGKLFVDYLSPLKRKRIQDKLAK
IKRFNEKQR

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=156, Percent_Identity=33.3333333333333, Blast_Score=85, Evalue=4e-17,
Organism=Escherichia coli, GI1789682, Length=168, Percent_Identity=72.6190476190476, Blast_Score=249, Evalue=6e-68,
Organism=Drosophila melanogaster, GI24645728, Length=138, Percent_Identity=30.4347826086957, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF2_VIBVY (Q7MGK6)

Other databases:

- EMBL:   BA000037
- RefSeq:   NP_936018.1
- ProteinModelPortal:   Q7MGK6
- SMR:   Q7MGK6
- STRING:   Q7MGK6
- GeneID:   2626101
- GenomeReviews:   BA000037_GR
- KEGG:   vvy:VV3225
- eggNOG:   COG0242
- HOGENOM:   HBG665227
- OMA:   RQLVDDM
- ProtClustDB:   PRK00150
- BioCyc:   VVUL196600:VV3225-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 19294; Mature: 19162

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: ACT_SITE 166-166

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLQVLTFPDDRLRTVAKPVEKVTPEIQKIVDDMIETMYDEEGIGLAATQVDIHQRIVV
CCCEEEEECCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHCEEEE
IDISESRNEPMVLINPEILEKRGEDGIEEGCLSVPGARALVPRAAEVTVKALDRDGHEFT
EEECCCCCCCEEEECHHHHHHCCCCCHHHHHCCCCCCHHHCCCHHHHHHHHHCCCCCEEE
LEADDLLAICIQHELDHLQGKLFVDYLSPLKRKRIQDKLAKIKRFNEKQR
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SVLQVLTFPDDRLRTVAKPVEKVTPEIQKIVDDMIETMYDEEGIGLAATQVDIHQRIVV
CCEEEEECCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHCEEEE
IDISESRNEPMVLINPEILEKRGEDGIEEGCLSVPGARALVPRAAEVTVKALDRDGHEFT
EEECCCCCCCEEEECHHHHHHCCCCCHHHHHCCCCCCHHHCCCHHHHHHHHHCCCCCEEE
LEADDLLAICIQHELDHLQGKLFVDYLSPLKRKRIQDKLAKIKRFNEKQR
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA